Rnf32 (NM_001289757) Mouse Untagged Clone

CAT#: MC227317

Rnf32 (untagged) - Mouse ring finger protein 32 (Rnf32), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf32
• Synonyms: 1700009J01Rik; 2700025B22Rik; 4930542N22Rik; Lmbr2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227317 representing NM_001289757
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAAGAAATAAGGATCGTTCATCTAACAAAGGTAACTTAGCAGTCACTGCAGTTGCCTTACAGGACC
  ACCTATTACATGATCTTCAACTTGGACATCTCTCGGTAGCAGATCCTTGTAAGATAAAAGCACGAAAGAA
  AGAGAAGAAGTCAAAATCTCTCAAAAGAGATGCAACAGCCATCATAGATACTGGGCTTAGAAAATCTACA
  GAGGGTCCTAACATGGAAGATCCAGAGAAGGAATATGTTCTAGACCCCACACCACCACCATTAACTTTAG
  CCCAGAAACTAGGCCTCTTGCCACCTCCCCCGTTGCCACTGTCATCAGATGAGTGGGAGAGAGTGAAGCA
  GAGATCCCTTCTACAAGGGGACTCCATTCAGCCATGCCCAATATGTAAAGAAGAATTTGAGCTCCATCCC
  CAGGTGCTGCTTTCCTGCTCACATGTATTCCATAGGGCATGCCTTCAGGCTTTTGAAAAGTTCACAAATA
  AAAAAACCTGTCCCCTCTGTAGAAAGAACCAATATCAAACAAGAGTGATACATGATGGGGCTCGCCTGTT
  CAGAGTAAAGTGTGCCACCAGGATCCAAGCCTATTGGCGAGGGTACATCGTTAGAAAGTGGTACAGAAAC
  CTGAGGAAAATAATCCCGCCTTCGGATGCCAAATTAAGGAGAAAGTTCTTTGAGGAAAAGTTCACAGAGA
  TCAGCCACCGAATCCTGTGCTCATACAACACCAACATAGATGAACTCTTCTCAGAAATTGATGTCTGCTT
  GGCGGTAAACCGTAGCATTCTTCAGCAGCTAGATGAAAGGTGTGGCCAGATAATCACCAAGGAGGACTGG
  GAGAAAATCCAAGCACAGGCTGCTCACCATGAGATCTATGAATGTTCCATCTGCCTGACTCCACTCTCCT
  TTCATGGCGATGGCCGGCAAGCAGCCATAGGAACCAGCAGCCAGCGTCCCAGAGAGACAGTCCTCCTGTC
  CTGTGCACATCTGTTCCACAATGCCTGCCTCCTGGCTTTGGAGGAGTTTTCTTTAGGAGACAATGCCCCT
  TTCCATGTCTGTCCTCTCTGTCGCTCCTGCTACCAAAAGAAAATTGTTGAATGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289757
• Insert Size: 1107 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289757.1, NP_001276686.1
• RefSeq Size: 1606 bp
• RefSeq ORF: 1107 bp
• Locus ID: 56874
• UniProt ID: Q9JIT1
• Cytogenetics: 5 14.78 cM
• Gene Summary: May play a role in sperm formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein.