Pcnt (NM_008787) Mouse Untagged Clone

CAT#: MC225430

Pcnt (untagged) - Mouse pericentrin (kendrin) (Pcnt), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcnt
• Synonyms: AW476095; C86676; KEN; kendrin; m239Asp; m275Asp; Pcnt2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225430 representing NM_008787
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACGAGCAGGAGCAGCGGCGCAGGAAGGTGGAGGCTGGGAGAGCCAAGCTTGCTAACTTCCGAC
  AGAGAAAAACAAAAGGTGACTGTCCGAATTCGAAGAAAAAGACGGCGAAGAGGAAGGGTTCGGCTGTCCA
  TGCGTCTGTCCAGGAGGAGGGTTCGGTAGCCACTCCCAACAGCGAGCTCCCCCAAGGAGGGGCGGTCTTT
  GAAAGCCCATCATGCAGCAACACCCTGGAGGGGACCAGAGGGGCATCTGCAGCTCAGGAACAAGAAGACT
  GTGAACTGGATGTGACTGACCTGCAGGGACAGCAGCAGACACAGCCACCGCCACCCCAGACAGCCCACAG
  CCTGGAGCTAGAAGCGCTGCGCTTGAGCCTGAACAACATGCACACAGCGCAGCTGGAGCTGACGCAGGCC
  AACCTGCAGAAGGAAAAGGAGACAGCGTTGACGGAGCTTCGGGAAATGCTCAATGGCCGGCGGGCACAGG
  AGCTGGCCCTGCTGCAAAGTCGGCAGCAGTGCGAACTGGAGCTGCTGCGAGAGCAGCACGCCCGCGAGAA
  GGAAGAGATGGCGCTCCGCAGCGGGCAAGAAGCAGCTGAGCTGAAGGAGAAGTTACGGTCAGAAATGGAG
  AAAAACGCCCAGACGATCGAGACCCTGAAGCAAGATTGGGAATCCGAAAGAGAGTTATGTTTGGAAAACC
  TCCGCCAGGAATTGTCGTTGAAGCATCAGTCGGAGATGGAGGGTTTGCAGAGCCAGTTTCAGAAGGAATT
  GTCAGAACAGAAGGTTGAATTGGAGAAGATTTTCCAGGCCAAACATGAGGCTGAAGTTTCCCTGAAGAAC
  CTGGAGGCTCAACACCAGGCGGCCATCAAGAAGCTGCAAGAAGACCTGCAGTCAGAGCACTGCCAGTATT
  TACAAGATCTAGAGCAGAAATTCCGAGAAAAGGAAAAAGCAAAAGAGTTGGAGTTAGAGACCCTTCAGGC
  ATCCTATGAAGACCTAAAGGCACAGTCCCAAGAAGAGATCAGACTCCTTTGGTCCCAGCTTGAGTCTATG
  AAAACCAACAGAGAGGAACTGAATGGGTCATGGGATCCAGTGCTGGCCCAGGCCTCTCACCTGGAGGAGC
  TGGAGCACCTGCGGTCGGGTTTCGCTCAGCAGCAGCAGCAAGAGCGAGCCCAGCACGAGTCTGAGCTGGA
  GCACCTGAGGGTCTATTTTGAGAAGAAGTTGAAGGATGCCGAGAAGACATACCAAGAAGACCTGACTGTG
  TTCCAGCAAAGGCTGCAGGAGGCAAGGGAGGATTCTCTGGAGTCTACAGAAATCAGTTCGTCCTGTGTGC
  TTCCGGAGGAGACATCTGGGAGAGAAGGAAAGGAGCCACCAGATCCACTTGATCTTCAGCTTGGGCAGCC
  GAAGGTCCAGGAGAGTCTGGTTGAAGACTGTCAGGTGAAACTCAGTAAAGCCGAGGAGAAGATTCAGCAA
  ATGAAAGAGGAGTTCCAAAAGAAGGAAGCCGAGTGGGAGCTTTCCCGGGAGGAACTAAAGAGAGAAGCTG
  AAGAGAGATTAGCCTCCATGTTTCTGGAGCTGAGAGAAAAGGCTGAGTCTGAGAAACTGTCCATAATCAG
  CAGGTTTGAACATCGGGAGTCCAGCATGAGGCACCTGCAGGACCAGCAGGCCGCCCAGATCTTAGACCTG
  GAGAGATCTCTGATGGAGCAGCAGGGTCACCTGAGGCAGCTGGAACAAGAACTCACTCGAGATGACCTTC
  TGCCATGCAGCCAGTGTGGCCAGGAGCCCGCCATGGCCCAGGAGGAGAAGAATGGCGCGCTCCTCCGAGA
  GAAGGAAGACTGTGCTCTGCAGCTGCTGATGGCCCAGAACAGGTTTTTGGAAGAGCGGAAAGAGATCATG
  GAGAAGTTTGCCAAGGAGCAAGATGCCTTTCTTCGAGACGCCCAGGAGAAGCACAACCACGAGCTGCAGC
  TCCTTCAGCAGGGACACCAGCAGCAGCTCCTGGCTCTGAGGATGGAGCTTGAAACCAAGCACCGCTCTGA
  GCTCACAGAGCAGCTCGCCTCCTCCGAGAGCAGGCGGCAGGCGCTCCTTGAGACTCACGTGGCCGAGCTG
  CAGGTAAAACACAATGCTGAAATTAGCGCCTTGGAGAAGAGACACCTCTCCAACCTGGATGAGTTAGAGT
  CCTGTTACGTGGCCGACGTTCAGACTATTCGGGATGAGCACCAACAGGCTTTGGAGCTCTTGCGAGCGGA
  GCTGGAGGAGCAGCTACAGAAAAAGGAGTCTTGTCACAGAGAGATGTTGACGCAGGAGCTGGAAAACCTG
  AAACGCCAACATGCTGAAGAGCTTCAGTCCGTAAGAGATAGCCTGAGAATGGAAATGAGTGCCCAGCACA
  TAGAGAACGGGAAAGGGCCGGCCGCTGACTTGCAAGGAGCACACCAGCAAGACCCGGCCATGGCTCTGCA
  CAATGAAGGGCACCTGCTGGTGGAGGATGGCGATGCAGTCTTGCGTAGTGTGGACGCTGAGGGCCTGCTG
  CACCAAGCTGGACCTCAGGAGCTGGGAGACGCGCACACAGTTGAAATGCAGAAGTCACAAGCAGAGTTGG
  CCAAACCCCAGGAGCTGCAGGCATCACAGGACCAGGTTGCACAGGTGAGAGACAAGGTCTTCCTGCTGAA
  CCGGGAGTTAGAAGAGTGCCGAGCGGAGCTGGAGCAGCTACAGCAGAGGCGGGAACGGGAGAACCAGGAG
  GGCACCACGCTGATCTGCATGCTCAGGGCTGACCTGGAGCTGGCTCAGGGCGAAGGGAAAGCCCTCCGAG
  ATGCCCTGAGGAGACTTCTCGATCTGTTTGGGGACACACTGAAGGCAGCTGTGACCCTAAAGAGCCGGAT
  CAGTGAGCGTGCGGGGCTCCTCCTGGACCACGAGGATGCTGCAGATACAAGTGACGCCCGGTTAGCAGCT
  GCCGCCCTTGGCGATATGTGGTCTGACGAGGGTCTACTGGAGATAGACAGAACGTTGCCAGAAGGGGCAG
  AGACATCTTCCGTGTGTGAAATTAGCAGTCATGTGTGTGAAAGCTTCTTCATCAGCCCTGAAAACACACT
  GGACTGTGAGCAGCCAATTAGGAGAGTCTACCAGAGCCTCAGCACGGCCGTGGAGGGCCTCCTGGAGATG
  GCCCTGGACTCCTCCAAACAGCTGGAGGAAGCTCGCCAGCTCCATCGCTGTGTCGAGAGAGAGTTCCGTC
  ACAGGAACGAGGAGATGGCCCAGGCTATGCAGAAGCAGCAGGAGTTGCTGGAGCGCCTGAGGGAGGAGAG
  TGCGGCCAAGGACAGGCTGGCTCTGGAGCTGCACACGGCTAAGGGCCTCCTTGAAGGATTCAAGGTGGAG
  AAAGTGGATCTGCAGGAGGCGCTGGGCAAAAAGGAGGAGTCGGAACAGCAGCTGATCCTGGAGCTAGAGG
  ATCTGCGGAAGCAGCTGCAGCAGGCTGCCCGCGAGCTGCTCACTCTGAAGGAGGAGAAGTCGGTCTTGTG
  GAACCAGAAGGAAACTTTGACTAATGAAGCAAAAGAAAGAGAAGCTGCACTGCAGGAGGAGGTGGAATCT
  CTTACCAGAGTCCAGTGGGAGTCCAGGAAGCAGTCTGAAAAGGACCGCGCCACCCTACTCTCTCAGATGC
  GCGTCTTGGAGTCTGAGTTGGAGGACCAGCTTGTGCAGCACCGGGGCTGTGCCCAGCTGGCTGAGGAAGT
  GGCTACCCTGAAGCAGCAGCTGGCTGCCCTAGACAAGCACTTACGTAGCCAGCGTCAGTTCATGGATGAT
  CAAGCTGCCGAGCGTGAGCATGAGCGGGAAGAGTTCCAGCAGGAGATCCAGAGGCTGGAGGGGCAGCTCC
  GCCAGGCAGCCCGGCCACGGCCTCCCGGGCCCCGGGACAGCCAGTGCGTCCAGTTAGACGAGGAGGTGGA
  GTTGTTACAAGAAAAACTGAGAGAAAAATTAGATGGGTTTAATGAATTGGTCATAAAGAAAGATTTTGCC
  GATCAACAATTGCTAATCCAAGAAGAAGAAATTAAGCGCCTCGAGGAGACAAATGCCAGCATACAAAGAC
  AAATGGTCCAGCTCCAGGAAGAACTGGAAAAACAGAAGAAAAGCATGGAAGAATTAAAGGAAAAAGAGAT
  ACTAAAGCAAGAGAACATGGGTGACTTACTGCTAACAACTGTATCACGGTCCGGGCTGGATGAAGCTGGG
  TGTCCTATGCTTCCCCAAGGCAGCTCTTCTAGGGGTCCGGAAGCCCAACCAGATGTAACAGAGAGGGCAC
  TCTTACAGCACGAGAATGAGGTTGTCCACAGGAGAAACTCTGAGATTGATGAGCTGAAAAGCCTCATCGA
  GAACTTACAGGAAAACCAGAGGCAATTACAGAAGGATAAAGCAGAGGAAATCGAGCAGCTCCACGAAGTC
  ATTGAGAAGCTGCAGAGCGAGCTGAGCCTCATGGGACCCAAGGTGCACGAAGTCAGCGATCCTCAGGCTG
  GCAGCCTCCACAGTGAGCTGGCCTGCCTCCGGGGGGAAGGCCTGGGTGGACAGGCACTACGGAGTGAGCT
  GCAGGCTGCGCAGGCAGCCAAGGAGGTGTTTGGCCAGCTCCTGGCAGACCAGGCCCACGGGCACAGCCAG
  GCTCTGGAGGCCCTGCAGCAGCGTCTCCAGGACGCAGAGGAGGTTGCTGCAAGACACCTGGCTGAGCTAG
  AACACTGTGTGGCCCTCAGAGAGGCTGAGGTGGAAGCCATGGCCTCTCAGATCCAGGAGTTTGCAGCTAC
  CCTGAAGGCAAAGGAAGCAATCATCGAGCAGAGAGATTTAGAGATTGATGCCGTGAACAAGTGGAAAGTA
  AGCCATTCCCTGGAGCTGGAGGCCATCCTTTTGGCTTTGGCTCACTTCCGTCATGCTCTGGAACAGCAGA
  CCTGTGCCACCCCGGACGAGCCCCCTGAGCTCCGGCAGCTGCGAGTGCAGTGTGCTCGCCTCAGCCACCA
  GCTACAAGTGCTGTATCGGCCTTTCCTCAAGTGCCGGATGCAGTTGGACCAGCACCAGCCTCATGTGGCA
  TCTATTGGCTGTGCAAACCCTTGTGCAGATGATGAACTGGAGCAGGAGGGAGTCAGCAACAGGCTGGCCT
  TGGCTCCCCACAGCCTGGCCGCACAGGCTAAGGAAGAACTGGAAGACTGTCCCCTGGGCAAGGCCAATCT
  CATGGCCCAGGTGAGACAACTTCAGGAGGAACTGGACCACCGCGTGCATTCCGTGGCCTCCAGGGACACC
  AACTCGGAAACTTGTAAATTACAGCAGCCAAACCTGTCGGAAAATGGGCCACGTAATCATTGTTGTAACG
  GTGAAGAAAGTAAGCCATCGCCTCCCGATGATGTACTTAATATTGCCAAAACCACATGGGATGTAATTGA
  TATTATTAAAAATCAGGATTTGTTGGTCCAAGTTGAAATGCCAGATTTCCCCACTCAAGAAAAGTTAACA
  TCCCAAGGTGGACCTTTCTCTTCCCAAGCCAGTGGGCACAGCGGCTCCCTCCTCCCAGAGGAGGCGGCTG
  AGCCCCAGCAGGACCCCGTGAGGGCACTGGACCTGTCGTCCTGGAGCTCCCCTGAAGTTGTCCGGAAGGA
  CCCATCCTTGGAGCCTCAGCACAGCCTGCCCCTGACCCCGGGCGTGGGCACAGTGAGCCTGCACAGTGTG
  GACATCTCACCAGACTGGACAGACCCGCTGCTGCAGGCAGACGTGTCGGGGCTGCTTTGTTACCCGGGCA
  AGTCTGCATCAGGCCAGGCCCCACTGTGGGCAGTGGCCCCCTCGGCTGGGAAGCACCATGCGGAGAGGAC
  AGCCACGGAGAAGGACGTCGAAGACTTTATCGTAACATCTTTTGATTCTCAAGAATTGTTAACATCCCCT
  TCTCATGAGTTAGCGAGAAGAAGCGATGGGAGCAGGAAAAGTGATGGCCCAGACATTGCTATGATGTTGA
  CCCTGGGCTCAGAAGGGTCTGAGACTCCCACCACTGACCTAGTCGCTGCTGCTGCTGCTGCTGTTCCTTT
  CTCTAGAAGGTTTGTCCAGTCACCAGGAGCAATGAAGGAGAAGGAGATCCATGCAAAGCAAATGAAGGCT
  TTGCTGCAGATGGTGTTTGATGAGAGCCACCAGATACTGGCCTTGTCAGAAAGCCAGGACCCCTCTAGTG
  CACTGAACAAAGGTGAACCACGTGACCCCCTGGATGGCTTCCCGAGAGACAGTCAGGCCCTTTCGGAGGT
  GACAACTGACAAAGGAGAGAAGGAATCTTTGGAAACACACCTTACGTGGAGTGAGGAGCTTTTGCGGGCC
  ATACAAGAAGTGTTTGCCAGGGAGCAGGAGAAGGCTGAGCTGCAGCCTAGACCTTATGGTTCAAACCTAG
  GAGATTATAACTCCCTGGTCCAGAGGCTGGAGAAGGTTATCCAAGAGCAGGGAGACCCGCAGAAGGTCCA
  GGACCACCTGTGTCTGTCAGACAGGAGCAGCCTGCTAGCTGAGATTCAGGCCCTGCGTGCTCAGCTCCGC
  ATGACACACTTACAGAACCAAGAGAAGCTGCAGCAATTGTGTGCAGCCTTGACGAGCACAGAGGCCCGAG
  GGAGCCAGCGGGAGCACCAGCTACGAAGACAAGTTGAACTTTTGGCGTACAAAGTGGAACAGGAAAAGTG
  CATAGCAAACGAGTTACAGAAAACCTTGAGCAAAGAACAGGAGACGGCGAGCGACGTGCGCAAGCGCCTG
  GTGGTGGAGCAGAATGCCGTCCAGGACTTGAAGTCCGAGCTGCACGCCTGTAAGCAGGAGAACACCAGCC
  TGCTGGAGTCGCTGGACAAGGTGCAGCAGGAAGTCCTGCGGCTCAGGTATGGCCATGTGACGTGGTCCTA
  CTGTGGAACTTCTTCTGCTTCGGCTGGCAGAGCTGTGCTGGACGGGAAGGAGAAGGAGCTGAAGGTTGTG
  CTGGAGGAGCTGGAGTCTGAGCGCGGAAAGGGGCAGGCCCTCCAGGCCCAGCAGGAGGAGCAGCAACTGC
  GGTACCTGCAGAGGGAGGGCCAGAGCTCCAGGGCCCTGGAGGAGTTGAAACTATCTTTGGAGAAGCAGCT
  TGCTCAAAACAACCAACTGTGTGTGGCTTTAAAACACGAGCGTGCGGCCAAAGACAACCTGCAGAAGGAG
  CTGCAGATCGAGGCCTCGCGCTGCGAAGCGTTGCTGGCACAGGAGAAGGGCCAGCTCTCAGAGCTGCAGA
  AGAGCCTGGAGGCTGAGCGGAGCCGCTCACTCGAGCTCTCAGAGGCCCTACAGCACGAGCGCCTCCTGAC
  AGAGCAGCTGAGCCGCAACTCGCAGGAGGCCTGTGCCCGCCAAGAGACACAAGTGCAGCACGCTCTGCTG
  CGAAAGCTGAAGGCAGAGAAGACTCGTGCGTTGGAGCTGGAGGCCATGCTTGAAAAGGTGCAGAAACAGG
  CTGCCCACACCCAGCAGCAGCTGGAGGCTCAGGCTCAGGAGCGCTGCGTAGAGCTCAGGAGAGAGAAGGA
  GCGAGAGTTGGAGATACAGCGTCAGCGTGATGAGCATAAGATCGAGCAGCTTCAGCGTCTGGTGAGGGAG
  CTGAGGTGGAAAGAGGAGGTGTCCGGAGGAAACGGCCCTTGCCGGGGCTCCCCCGGCCGGGGTTCTCTGG
  AGAGGGACCAGTTCCAGGAGCAGCAACAGGAACTGGAGAAGATCCGGCAGCAGCTACTGTGTGCAGCTGG
  CCTCCTCACAAGCTTTACCAACCACACGGTAGACAGGACAATTAAAGATTGGACGTCATCAAATGAGAAG
  GCGGTGTCATCCTTAATGCGCACGCTGGAGGAGCTGAAGTCTGAGCTGAGCATGCCCACCTCCTTTCAGA
  AAAAGATGACTGCAGAACTCCAGGTCCAGCTCATGAATGAACTGCTGAGCGACAATGATGCCCTCACAAA
  AGCTGTGGGCATGGCAACCCGAGAGAAAGCTGAGCTGTGCAGGACCGTCTCCCGGCTCGAGAAGACCCTG
  AAGCACCACACGCAGAAGGGCTGCGTTCTTAATAGGCAAAGCAAGTCTTCATTGAAGCAAGATGGGACAG
  ATTTGCAGAGTTCTCTACGGCACTCAGACCCAGAGTGGCACTCGCAGACTACAAGTGGGGACACAAACAC
  CTGCAATATCAAAATGGAAAAGCTGTACCTGCACTATCTGAGAGCAGAGAGCTTTAGGAAAGCTCTGATT
  TATCAAAAGAAGTATCTCTTACTGCTGATTGGTGGATTCCAGGACTCAGAGCAGGAAACGCTCTCCATGA
  TCGCTCACTTGGGGGTCTTTCCTTCCAAAGCAGATAAGAAAATTACTATGTCACGTCCCTTCACCAAGTT
  CCGCACCGCCGTTCGAGTGGTCATCGCAGTATTGAGGTTGCGGTTTTTGGTTAAGAAGTGGCAGGAAGTG
  GATCGGAAGGGAGCCCTGGTACACCCCAAAAGCACCCGCCACGGACACCGAACATCCCAGAGACAACGTT
  CTCCATCAGGACCCAGAGCGTCTCTGCCAACCCGAGACACTTCCTCAGGGCCTACCAAGGCCTCTCGGCA
  CAGCCCCAGATCTGCGGCAGCCGGCTCACCGGGCAAGGAAAGATCAACTTCGACACCAAGTTCAAGGCTA
  GAAAGATCCCTAACTGCTTCTCAAGATCCTGAGCACTCCTTGACAGAATACATCCATCATTTAGAAATGA
  TCCAGCAAAGACTTGGGGGGTTGCCGCCAGATTCTACTCAGAAATCCTGCCACCAGAAGATTAAACAGTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008787
• Insert Size: 8751 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008787.3, NP_032813.3
• RefSeq Size: 9530 bp
• RefSeq ORF: 8751 bp
• Locus ID: 18541
• UniProt ID: P48725
• Cytogenetics: 10 C1
• Gene Summary: Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a).