Kntc1 (NM_001042421) Mouse Untagged Clone

CAT#: MC225227

Kntc1 (untagged) - Mouse kinetochore associated 1 (Kntc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kntc1
• Synonyms: D330012D13; jgl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225227 representing NM_001042421
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAACAATATTGAGCTGCTAACAAGCGATGACACTGGAAGTGGATGCCTGAGTGTTGGCTCAAGAA
  AAGAAAACGTGACTGCACTGTACCAAGCCGACTTGCTTGGGAAGATCTCCTCTGAGAAGACCTCACTGAG
  TCCGAAGATCCAAGCATTCAGCTTAAGCCATGGGTTTATAATCGTTGCAGACCAGTCAGTGATATTGCTT
  GACAGTATATGCAGATCACTCCAGTTGTTTCTTATCTTCGATACTGACGTGGATGTGGTCGGCCTGTGTC
  AAGAAGGAAAGTTTCTCTTTGTGGGGGAGAGAAGCGGCAACTTCCACCTTATCTACGTCACATCGAAGCA
  AACGCTGTTCACCAAGGCTTTTGTGGAGAAAGCTTTGGACGAGAGTCAGCGAACGTACCGGAATCTCATT
  ATTGAGAAAGATGGTTCAAATGAAGGTACCTATTATATGTTGCTTCTAACGAACAATGGATTTTTTTATA
  TTACAAACCTCCAGCTTTCACAAATTGAACAGGCCATTGAGAACACGGACTTGGATTCAGCCAAGAAGTT
  ACAAGGACAATTCAAATGTAGTTTTATTTCTACTGAAAATTACCACAGTTGTCTCAGTCTTGTGGCATCT
  CAATCTGGAACATTTGCAAGTAAAACTTCTGTGATAATTGGGGGAACCGGTAGTTGTGCATTCTCAAAAT
  GGGAACCCGATTCTACCAAGAAAGAAATGTCTCTTAAGAACTTTGTGGGCACAGACATTATTAAAGGTGC
  AAAGTCATTTCAGCTGATCGACAACCTGCTTTTTGTGCTGGATACTGATAATGTGCTGAGCTTGTGGGAT
  GCTTACACTCTCACTCCTGTGTGGAACTGGCCCTCTCTTCCCGTAGAGCAGTTTGTTCTCACGACAGAAG
  CGGATTCTCCCTCATCTGTTACCTGGCAAGGAATTACAAATCTCAAATTAGTCACCCTGACAGCAACAGC
  GAAGGAGAAGATGAGAAGCCTCATCATTTACTCTCTGCCTTCGATGGAAACACTGTACTCTTTGGAAGTA
  TCTAGTGTTTCTTCTTTAGTTCAAACAGGAATCAGCACGGACACTATATATCTTCTGGAAGGAATTCACA
  AAAATGATCCTAACCTATGTGAAGACTCTGTGTCTGACTTAGTACTTAGGTACCTCACAGAGGTGTTACC
  AGAAAACAGACTGAGCCGGTTACTTCACAAGCACAGATTTGCCGAAGCCGAGAGCTTTGCCATTCAGTTC
  GGACTGGATGTTGAGCTTGTTTATAAGGTCAAATCAAATGATATGTTGGAGAAGCTGGCTTTGATTTCCT
  CGGACAAGAGTGAGCAGTCCAAATGGCAGCAACTTGTAGATGAAGCTAAAGAAAACCTATGCAAAATCCA
  GGACGATGACTTCGTGGTGAATTTCTGCCTCAAGGCCCAATGGGTAACCTATGAAACCACCCAGGAAATG
  CTCAGTTATGCCAAAACCAGGCTCATGAAGAAAGAAGACAGAGCTCTACCTGCCTCTTCTGATGCCTTCA
  TGGAGGTGCTAAAAGCTCATGCAAAGTTAACTACATTTTATGGAGCATTTGGACCAGAAAAATTCAGTGG
  TAGCTCTTGGATTGAGTTCCTGAATAACGAAGATGACCTCAGGGATGTTTTTTTGCAGCTAAGTGAAGGA
  AACTTCGCTTGTGCTCAGTATCTTTGGCTTAGACATCGGGCTGACTTTGAAAGCAAGTTTGACGTGAAAA
  TGCTGGAGAATTTGCTGAATTCAATATCTACACAATTCCCCTTGGAAAACCTTTGTTCCTGGTTTAAAAA
  TGAAGTGATTCCGTTTGTGAGGAGGATTGTGCCCGAAGGACAAAACATTCTTGCAAAATGGCTAGAACAA
  GCATCAAGGAACCTAGAGTTAACTGATAAGGCAAACTGGCCAGAAAATGGACTTCAGCTGGCAGAGGTGT
  TCTTCACTGCAGAAAAAACAGACCGGTTTGGATTCGCATCTTCTTGGCACTGGATTTCCTTGGATTATCA
  AAACACAGAGGAAGTCCGCCAGTTACGGACACTGGTGAGCAAGTTGCGGGAGCTGATCATTCTGCATAGG
  AAATACAACTGCAAGCTGGCCCTCTCGGACTTTGAGAAGGAAAATGCCACCACTGTGGTGTTCCGCATGT
  TCGACAGGGTCTCGGCCCCAGAGCTGATCCCTTCGGTCTTAGAGAAGTCTGTGAGAGTTTACATTCGCGA
  ACAGAATCTGCAAGAGGAGGAGCTGCTGTTGCTCTATATAGAGGATTTACTGAAGAGGTGTAGCTCAAAG
  TCAATGACTCTCTTCGACACAGCGTGGGAAGCAAAGGCCATGGCCGTGATAAGATGCTTGTCTGACACAG
  ATCTCATCTTTGATGCTGTGCTGAAGATAATGTACAAGGCGGTGGTTCCCTGGAGTGCAGCCGTGGAGCA
  GCTGGTGAAACAGCACCTGGAAATGGACCACCCCAAAGTCAAGTTATTGCAGGAAAGCTACAAACTGATG
  GAGATGAAGAAACTTTTGCGAGGGTATGGGATAAGAGAGGTGAATCTCCTGAACAAGGAAATAATGAGAG
  TGATCCGATACATTCTAAAGCAGGACATCCCGTCTTCTTTAGAAGATGCTCTGAAGGTGGCCCAGGGGTA
  CAGGCTGTCTGATGACGAAATCTACAGCCTGAGAATCATCGATCTGATTGACAGGGAGCAGGGGGGAGAT
  TGCCTCCTGCTACTGAAGTCTCTGCCTGCCGCTGAAGCCGAGAAGACTGCAGAAAGGGTTATCATCTGGG
  CTCGACTGGCACTGCAGGAGGAGCCTGACGGCTCTGAGGAGGACAAGGCCTGGAGAATATCTGTGGCGAA
  GACATCGGTGGATATTCTCAAGATCCTGTGTGATATTCGAAAAGACAATCTGCAGAAGAAGGATGAATCT
  GAAGAATTCCTGAAGCGATTTCAAATGGTTGCTAGTTTACAGGAGAACTTTGAGGTCTTCCTTCCATTTG
  AAGACTACAGCAACACCGCCTTGGTAGCTGGCCTTCGAGAACAGTATATTAAGGCACAGGAAGCTGCACA
  GGCTGAGCACAAACACAGGGGACCCCCCGGGCCCACCCCGGCTAGGGGGACACACCTGAGCATCAAGTCA
  AAGCTGCACCGACAGGCACTGGCCCTGCAGGTGTCTGAACAGGAGCTGGAGGCAGAGCTGACCTTGAGAG
  CCTTAAAGGACGGGAAGGTGGTGGCCGCCCTGAGCAAATGCAGAGATTTACTTAAGCATTACTGCAATGC
  CGACACTGGGAGGTTACTGTTTGTGGTGTGTCAGAAGCTCTGTCAGATGTTGGCTGACGATGTCCCAATG
  GTGGCCCCAGGAGGACTCAGCCTTCCTTCTGAGATCCATGACCTCGCATGCCACGCTGTCACCATCTGCA
  GTCCAGATTATTTGCTGGATGTTTTAGAACTAAGTAAATACACTTTAACCGCTGTGGAGCTTTGCAGACA
  ATGCCAGATGGATGACTGTGGAATGCTGATGAAGGCCGCTCTTGGAACTCACAAGGATCCCTATGAAGAG
  TGGTCCTTTAGTGACTTCTTCAGTGAAGATGGAATTGTTCTCGAGTCCCAGGTGGTGCTGCCTGTCATCT
  ATGAATTGATCTCATCAGTGATGCCCCCTGCAGAAAGCAAAAGACATCCTTTGGATTCTATAAGTTTGCC
  ATACTGCTCCACTAGTGAAGGAGAAAACCGTATTCTCCCTCTTGTAAGCTCCATCTCTGCTCTGCTCCGG
  AGCCTCCAGGAATGTAGTCAGTGGGAGCTAGCCCTGAGGTTCGTGGTGGGCTCCTTTGGTACCTGCCTGC
  AGCACTCTATGTCAAACGTCATGAGTATCAGCTTGAGTAAACAGTTACTTGGGAAGAACACATTGGCGAA
  CTCCAGGCATATCATTATGGAATTGAAAGAGAAGTCCATCACATTTATCAGAGAAAATGCTACGACACTC
  CTGCATAAAGTGTTTAACTGTCGGGTGGTGGATCTGGACCTCGCCTTGGCTTACTGCACTCTCTTACCTC
  AAAAGGATGTATTCGACAACCTCTGGAAGTTCATAGATAAAGCGTGGCAGAATTACGACAAAATTCTGGC
  ATTATCTTTGGTGGGCTCTCAGCTGGCAAATCTCTACCAGGATATTGAAACAGGACTTTGGTTCCACGAG
  CTCAGTATCGATGCCAAGTGGGGTATTCGTCTTGGTAAACTTGGTATTTCTTTTCAGCCTGCTTTTAGAC
  AAAATTTTCTCACCAAGAAAGACCTTATCAAAGCTCTTGTGAATAACATAGACATGGACACCAGCCTCAT
  TCTGGAGTATTGCAGCACATTTCAGCTGGACTCTGACGCTGCCCTTCGGCTGTTCATTGAAACTCTGCTC
  CGCAACACTAGCAGCCAGAGCCAGGGAGATGCTGCCCCGGAGTCCACAAAGCATCAGCATTCCAAACTCC
  TGGCGAAAGCCACTGAGCTGGTCCCTTTACTGAAGAACACAAAGGATCTGGTGATCAGTCTTAGTGAGAT
  CCTCTATAAGCTGGACCCATATGATTATGAAATGATTGATGTTGTCCTGAAAGTTTTAGAACAAGCTAAC
  GAAAAGATAACCAGTGTGAACATCAATCAGGCGCTGAATCTTCTGAGACACTTGAAGTCATACAGAAGGA
  TCTCCCCGCCAGTGGACCACGAATACCAGTACGCGCTGGAGCACATGATCACTCTGCCACCGGCTGCTCA
  CACTAGACTGCCCTTTCACCTAATACTGTTTGGCACAGCACAGAACTTCTGGAAAATTCTCTCCTCAGAG
  CTCAGCGAAGAGTCGCTGCCAACCCTGCTCTTAATCGCGAAGCTGATGAAGTTCTCCCTAGATACTCTCT
  ATGTGTCTACCGCCAAACACTTGTTTGAAAAAAACCTGAAGCCAAAGCTCCTGAAGTCGGCACAAGCCAG
  GTCCTCCACCCTGATGAGCAAGGAAGTGGATAAGCTCATGCAGACGCTGGAGTCGTACCTCCTGTCCATC
  GTCAACCCCGAGTGGGCTGTCGCCATTGCCATCAGCCTCACCCAGGAAGTCCCAGAAGGCCCCTTCAAGA
  TGTCTAGTTTGAAGTTCTGCCTGTATTTAGCTGAGAGATGGCTACAAAACATCCCACCTCAGGATGAGAC
  GTGTGAAAAGGCCAAAGCCTTGCAGAAGAAGCTCTGCCTCCAGGTCCGGCTCTCAGGAACGGAAGCCGTG
  CTCATAGCCCACAAGCTGAACGATCAGGAATATTTAAGAGTGATAGGGAAGCCAGCGCACCTCATCGTGA
  GCCTCTACGAACATCCCAGCATAAGCGAAAGGCTTTGTACCACATCTGGCAAAGATTATCCCGATATCCA
  TACAGCCGCTAAAGAAATAGCCGAAGTCAATGAAGTTAATTTGGAAAAAATCTGGGACATGTTGTTGGAG
  AAATGGCTGTGCCCCTCAACGGTGCCCAGTGAGAAAGCATCGGAGTTCTTTGAGCTGGAAGAGGATGAAG
  TGCTGCATAGAGTCGTGTATCTCCTCCAGGCTCGTCCTGTTGATTATTGTTCGAGAATGCTGTTTGTATT
  TGCCACTTCAGCTACAAGTACACTGGGCATGCGCCAGCTAACGTTTGCCCACAAAGCACGAGCTCTGCAG
  TGTCTCCTCTATTTGGCTGACAAGGAAACAATTGAATCTCTCTTCAAAAAGCCCATTAAAGAAATGAAAT
  CCTATCTGAAATGTATAACTTTCCTGGCATCATTTGAGACATTGAATATTCCCATCACATATGAATTATT
  CTGCAACAGTCCCAAAGAAGGCATGATTAAGGGCTTGTGGAAGAACCACAGCCACGAACCTATGGCAGTA
  CGATTGGTTGCAGAGCTTTGCTTAGAATACAAAATCTATGACCTGCAGCTTTGGAATGGGCTCTTGCAGA
  AGCTTCTGGGCTTCAACATGATTCCTTACCTGAGGAAAGTGTTATCCTGCATTTCTAGCATCCATTCGTT
  ATGGCAGGTGCCCTACTTCAGCAAAGCGTGGCAGCGCGTGATCCAGATCCCTCTGCTCTCGGCTTCCTGT
  CCTTTAAGACCCAGTCAGTTAGCAGACTGCTGTGACAGTCTCGTCGCCATCCTTGAATGTCCAGTTTCAG
  ATGACCTGGACATGATGGGAGTCGCCAAGCAGTACGTCCAGTTAGACCTTCCCGCTTTTGCTCTGACCTG
  CCTGACGCTCATGCCCCACTCTGAAAAAAGACACCAGCAGATCAAGAATTTTCTGAATTCCTGTGATGCT
  CGGATCATTTTACAGCAGATAGAAGAACATATGAACACTGGCCAGCTAGCAGGGTTTTCACATCAGATTG
  GAAGTCTGGTTCTGAATCATGTTGTAAATAAGAAGGAATTTGGGATTTTGGCAAAGACAAAGTATTTTCA
  GTTGTTGAAATGCCACGTGATAAACACCGGCAACGTCACTGAATTGGTAAACTATTTGGCAAATGACTTC
  AGTGTAGATGAAGCATCAGCGTTGATAAATGAGTATTCAAAGCATTGTGGGAAGCCTGTGCCAGCAGATG
  CAGCTCCCTGTGAGATCCTGCAGACATTTCTTGGTGGATCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042421
• Insert Size: 6624 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042421.1, NP_001035886.1
• RefSeq Size: 6961 bp
• RefSeq ORF: 6624 bp
• Locus ID: 208628
• UniProt ID: Q8C3Y4
• Cytogenetics: 5 F
• Gene Summary: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (By similarity).[UniProtKB/Swiss-Prot Function]