Ralgapa1 (NM_001003719) Mouse Untagged Clone

CAT#: MC225162

Ralgapa1 (untagged) - Mouse Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ralgapa1
• Synonyms: 2310003F20Rik; 4930400K19Rik; AI563624; Garnl1; GRIPE; mKIAA0884; Tulip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225162 representing NM_001003719
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG
  CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGACCTTAAACAGTTTTT
  TGACCAACATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA
  CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC
  TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA
  CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG
  GATAACTGCAGCAAAGAACAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTCTCAGCACCACAATCCG
  AATATGGACCTCGGACTTTAGATAATCTTATTAATCCTCCACTCAGCCTTCAAGAAACTCAAGTAACTAT
  AGAGGAAGTCACTCCTCTTGTTCCGCCACAGTCAGGAGATAAAGGACAAGAAGACCTCACAAGCTATTTT
  CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG
  AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACATTTG
  CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGATCAGACCAAAGCCTCATTATGTGATG
  ATAAAGAAGGATGCTGAAACCAATGAAACAATATATTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTTA
  TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATTCAGGACCTAATATTCCTGGGAT
  GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTTTCCAGAGAAGAAAGCAAA
  AATGATACTGTTGATAAAGTAGACAAGTCTGCAGAGCCTGAGCAGTCTCATTCCAACACAAGCACTCTCA
  CGGAGCGAGAGCCTAGCTCTTCCAGTCTCTGCAGCATCGACGAAGAACATCTCACAGACATCGAAATTGT
  TCGCAGAGTGTTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA
  TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTGAAAGTGTATCAAGAATGGATTCAACAAGAGG
  AAAAGCCTTTGTTTATGCAAGAGCCTGAGGACACTGCGATCACTTGTTCAGATATACCCTGCAGCGAAAC
  TGTTGCAGACCATGACAGTGCAATAGAAGATGGAGAGAAAAGAGAAGAGGAAAATGGGACCAGTACTTCT
  GAACATGTTCGCAATTCTAGCTGGACAAAAAATGGTTCCTACCAAGAAGCTTTTCATGTCTGTGAAGAAG
  CCACAGAACAGAATATTCAAGCTGGCACTCAGGCCGTTTTGCAGGTGTTTATTATAAACTCATCGAACAT
  ATTTCTTCTTGAACCTGCAAATGAAATAAAGAATCTTCTGGATGAGCATACAGATATGTGTAAACGCATT
  CTTAATATTTATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTGCTCG
  TGCTGCTCAGAGTCACGGAGTCCGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA
  AAGCATGACCCTGGCGGGTCGACTTGCAGGACCACTTTTTCAGACTCTCATTGTTGCCTGGATCAAAGCA
  AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCGGTATTGTCATCATTGACCTATT
  GGGAAGAATTGGCCACTGAGTGGTCACTGACCATGGAGACACTGACTAAAGTTTTGGCTAGAAATTTATA
  TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA
  GGAGTTGGACACGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC
  CTGGCCAGGCACCTATGAGACAGAGAAGTGCAACGACCACTGGCTCCCCAGGAACTGAGAAGGCCAGGAG
  TATAGTACGGCAAAAAACTGTTGATATTGATGATGCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACAT
  TTTTCACAGAGTGAAGACACTGGAAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGAA
  GTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGAG
  CCCCAAACTGCCTCCTCTTAATAGTGAGACTGGTGGCAACAGCGCCAATGTTCCTGACCTGATGGATGAG
  TTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGGAGTAGTCCAGGCAGCC
  TTGAAATCCCGAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGGCCTGTTGATGACCCTGG
  TGTACCCTCCGAATGGACGTCTCCAGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTCG
  GATTCCTTCAGTGCCTTCCAGTGTGAGGGACGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGGA
  TCCCATCTTCTGCTGATGTGGATTTAGGTTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCCAG
  CCTAACTACGCTTCATTTAGATTCAGAAACAAGCAGCCTTAATCAGCAAGCCTTCTCTGCAGAAGTTGCA
  ACCGTTACTGGTTCAGAAAGTGCTTCCCCCGTTCATTCAGCTCTGGGATCCAGGTCGCAGACCCCCTCCC
  CATCTACACTGAGCAGAGCTCACATAGAACAGAAGGACCTACAGCTCGATGAGAAGCTCCACCACTCTGT
  TCTTCAGACGCCGGATGACCTAGAAATCAGTGAATTTCCATCTGAATGTTGTAGCGTGATGGCAGGAGGT
  ACTCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGCAGAATGCTGGGCATTTTGGGAGATG
  TCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCT
  AGCCAAGATCAGAGATAACCTTGGCATTTCAGCTGACAACCTGACTTCCCCTTCTCCACCCGTCCTGATT
  CCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGTA
  AATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCTCCAAATAGAGATTT
  TTTAACACATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATTGTCAATACGATC
  ATCAAGCACTGCTCACCTCAGTTTTTTTCACTCGGTTTGCCTGGGGCCACGATGCTTATTATGGATTTTA
  TTATAGCAGCTGGGAGAGTGGCTTCTTCGGCTTTTCTTAATGCACCAAGAGTGGAAGCACAAGTTCTTCT
  GGGATCTTTAGTTTGCTTTCCCAACTTATATTGTGAATTGCCTGCTCTCCATCCCAACATTCCTGATATT
  GCTGTGTCTCAATTTACAGATGTTAAGGAACTTATAATTAAAACTGTATTAAGCTCGGCAAGAGATGAGC
  CCTCTGGTCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATTTGGATTTGTGAAGAATTAGTCCACGA
  ATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAACA
  GTTGCCCACGTCGCCTGTAACATGCTTCACATGCTGGTTCACTATGTCCCTAGACTTCAGATTCACCAGC
  CGCAGTCTCCCCTGAAAATTATTCAGATCCTAATAGCAACTATCACCCACCTTTTGCCAAGTACAGAAGC
  TTCATCATATGAAATGGACAAGAGGTTGGTTGTCTCATTACTTCTTTGCCTTCTGGACTGGATCATGGCC
  TTACCTTTAAAGACACTGCTCCAACCAGTCCATGCTACAGGAGCAGAAAATGATAAGACCGAAAAGTCTG
  TTCTCAACTGCATTTATAAGGTATTACATGGGTGTGTCTATGGAGCGCAAAGTTTTAGCAATCCCAAGTA
  CTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGATCCGTTTATGCATTTGGAAAGTCTGAAA
  GAACCTGAACCTTTACACTCTCCAGATTCAGAGCGGTCTTCTAAACTGCAGCCTGTGACAGAAGTGAAAA
  CTCAGATGCAACAAGGATTAATCTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACTTGGG
  CCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCATGACAATCATTAC
  AGTGAAAGTACTGAACTTTCTCCTGAGCTCTTTGAAAGTCCAAACATCCAGTTCTTTGTGCTGAATAATA
  CAACCTTAGTGTCCTGTATCCAGATAAGATCGGAAGAGAGTATGCCTGGAGGAGGTCTAGCTGCTGGCCT
  TGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGCC
  ATACTGTATGGACCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTGACC
  AAGAGAAACCGGAAGAGCCTCCTACATCTAACGAATGCTTAGAAGACATCGCAGTAAAAGATGGGCTTTC
  TCTCCAGCTTAGAAGATTTAGAGAAACTGTACCAACTTGGTCTACGATACGAGAGGAAGAAGATGTTCTT
  GACGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAGTGTTTACAGAGAACGGGAATCTCCCTCAATG
  TTCCTGCTCCACAGCCTCTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGCA
  ATACACGGAAGAAAAAGAGTTTGTAGAGAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGAT
  GAGCCAACACCTCAGAAACCTCAGTCAGCTTTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATGA
  ATTCCTGGGACAAACGGAGGAGCTTTCATCTCTTGAAGAAAAATGAAAAGCTGCTCAGAGAACTTAGGAA
  CTTGGATTCAAGGCAATGCCGAGAGACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGAT
  AAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTAGCTGGCCTTGGTTGGG
  AGGTAAACCTTACAAACCACTGTGGCTTCATGGGAGGCCTTCAGAAGAACAGAAGCACTGGACTGACCAC
  TCCCTATTTTGCTACCTCGACAGTAGAAGTAATATTTCATGTATCTACACGAATGCCTTCTGATTCTGAT
  GACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTGCACATTGTTTGGTCAGAACATACTA
  GAGACTACAGGAGAGGAATTATTCCTACTGAGTTTGGTGATGTCCTCATTGTAATATACCCAATGAAAAA
  CCACATGTTCAGTATCCAGATCATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCACTTTTTGATGGTGCT
  ATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAAT
  CACTGATTCCATTGTATCAAAATTTCTATGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACCA
  TTTAGAGCCAACAACATTTGAAGACTTTGCAGCACAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCCT
  GCTGACGCAGTTGGATCCTACCCAGAGATTCCACCCAGTGACGCTGCCCCAGCAGCACAGGTAGACGGGG
  CTGACCTGGCCTCTCCCATGTCTCCTCGAACTAGCAAAAGCCGCATGTCCATGAAGCTGCGTCGGTCCTC
  TGGCTCAGCCAATAAATCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003719
• Insert Size: 6252 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003719.2, NP_001003719.1
• RefSeq Size: 8249 bp
• RefSeq ORF: 6252 bp
• Locus ID: 56784
• UniProt ID: Q6GYP7
• Cytogenetics: 12 C1
• Gene Summary: Catalytic subunit of the heterodimeric RalGAP1 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB (By similarity). May interact with the HLH region of TCF3/isoform E12.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region and an internal exon in the 3' region which results in an alternate downstream termination codon, compared to variant 4. The resulting isoform (3) lacks an internal segment but has a longer and distinct C-terminus, compared to isoform 4.