Evpl (NM_025276) Mouse Untagged Clone

CAT#: MC225135

Evpl (untagged) - Mouse envoplakin (Evpl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Evpl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225135 representing NM_025276
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAAGGGGCTGAGCAAAGGCTCCCAGGGGAAGGGGTCCCCCAAGGGCTCCCCAGCCAAGGGGTCCC
  CTAAGGGCTCCCCCAACAAGCACAATCGGGCTGCAACCCAGGAACTGGCTCTGCTCATCTCCCGCATGCA
  AGCCAACGCTGACCAGGTGGAAAGAGACATTTTGGAGACGCAGAAGAAGCTGCAACAGGATCGGCAGAAT
  GGTGAGCAGAACCAGGCTTTACAACACCAGCAGGAGACTGGCCGCAACCTGAAGGAAGCGGAGGTACTCC
  TCAAAGATCTCTTCCTGGATGTAGACAAGGCGCGGCGGCTCAAGCACCCACAGGCTGAGGAGATTGAGAA
  AGACATCAAGCAGCTGCATGAGCGGGTGACCCAGGAGTGCTCGGAGTACCGTGCTCTGTATGAGAAGATG
  GTACTTCCGCCTGATGTGGGGCCCAGGGTCGACTGGGCGCGTGTGTTGGAGCAGAAACAGAATCTGGTCC
  GTGAGGGCCACTATGGGCCTGGGATGGCAGAGCTGGAACAGCAGGTGGCCGAGCACAACATCCTGCAGAG
  AGAAATCGAGGCTTACGGGCAGCAGCTGCGGACGCTGGTGGGACCGGATGCAAACACCATCAGGAACCAA
  TACCGTGAGCTGCTGAAGGCGGCCTCGTGGCGCCGACAGAGTCTGGGCAGCCTGTACACCCACCTGCAAG
  GCTGCACGAAGCAGCTGAGCGCCCTGGCCGATCAACAAGGCCGCATCCTGCAGCAGGATTGGAGTGACCT
  CATGCCGGACCCCGCTGGCGTGAGGCGAGAGTATGAGCACTTCAAGCAGCATGAGCTGCTGGCCCAGGAG
  CGGAGCATAAACCAGCTGGAAGATGACGCAGATCGCATGGTGGAGCTTGGGCACCCGGCCATTGGGCCCA
  TCCAGGTCCACCAGGAGGCCCTCAAGATGGAGTGGCAGAATTTCCTAAACCTTTGCATCTGTCAGGAGAG
  CCAGCTGCAACGGGTGGAGGACTACCGTAGGTTCCAGGAAGAGGCGGACTCAGTCAGCCAGACCCTGGCA
  AAGCTCAGCTCCAACCTGGATACCAAGTACGGCTTTGGCACTGGGGACTCCTCCGGCTCCCCCACAGAGC
  TCCTGCTACAGCTGGAGGCAGAGGAGAAGCAGCTGGCCATAGCAGAGAGGGCTGTTGGAGATCTGCAGCA
  GCGAAGCCAGGAGGTGGCCCCACTGCCACAGCGCAGGAACCCATCCAAGCAGCCCCTGCACGTGGACAGC
  ATCTGTGACTGGGACTCGGGAGAAGTTCAGCTGCTTCGGGGAGAGCGGTACACACTCAAGGATAATGCTG
  ACCCATACACGTGGCTGGTTCAGGGCCCTGGAGGAGAGACCAAAAGTGCCCCAGCTGCCTGCCTCTGCAT
  CCCAGCACCAGACCCTGAAGCTGTGGCCAAAGCCTCTAGGCTGGCCACGGAGCTGCAGACCCTGAAGCAG
  AAGCTGTCTACAGAGAAGAACCGCCTGAAGGCTGCTGCTGTGGAACACTTACAGCCTGGCCAGCAGGCTC
  CGGCAGGCTCAGCCCCCGCTGACCCACAAGGTCAGACGCTCCTGTCACAGATGACCCAGCTGGATGGGGA
  TCTGGGGCAGATAGAGAGGCAGGTGCTGAGCTGGGCCCGGAGCCCACTGAGCCAGTCTTCTTCACTGAAG
  GACCTCGAGGGCCGCATCCACAGCTGTGAGGGCACAGCACAGCGCCTTCAGAGTCTGGGAGCGGAGAAGG
  AGGCAGCCCAGCAGGAGTGTGAGGCGTTTCTGTCTACCAAGCCTACGGGCTCCGCTGCCCTGCAACTGCC
  CGTGGTCCTCAACAGTGTGAAGAACAGATACAATGATGTGCAGAGCCTGTGCCACCTCTATGGGGAGAAA
  GCCAAGGCTGCTCTGGGCCTGGAGAAGCAGATCCAGGAGGCAGACAGGGTCATCCAAGGCTTTGAAGCCG
  CCCTGGCACTTGAGGGCCCCGTCCCCGAAGGCTCTGGGGCACTGCAGGAGAGGGTCAGCGAGCTGCAGCG
  CCAGCGCAAAGAACTCCTTCAGCAGCAGGCCTGTGTGCTGGGGCTGCACCGCCAGCTGAAGGCCACTGAG
  CATGCGTGCAGTGCGCTGCAGAACAATTTCCAAGAGTTCTGCCAAGACCTGCCGCGCCAGCAGCGCCAGG
  TTCGTGCCCTCACTGACCGTTACCACGCTGTGGGGGACCAGCTGGATTTGAGGGAGAAGATAGTACAGGA
  TGCCAGCCTCACCTACCAGCAGCTCAGGAACAGCAGGGACAACCTGAGCTCTTGGCTGGAGCAGCTGCCC
  CACCACCGGGTGCAGCCCAGCGACGGGCCAAGCCAGATTTCCTACAAGCTGCAAGCACAGAAGAGACTGA
  TACAAGAAATCCTGGGGCGCGAGCAGGACCAGGCCACCGTGTCCCGCCTCACCCGGGACCTGCAGGAGGC
  CCTCCAGGACTATGAGCTGCAAGCAGATACCTACCGCTGCTCCTTAGAGCCCGCCTTGGCAGTGTCCGCC
  CCGAAGAGACTCAGAGTGATTTCCTTGCAGGAGAGCATCCAGGCTCAGGAGAAGAACCTTGCGAAGGCTT
  ACACTGAGGTTGCCGCAGCTGAGCAGCAGCAGCTGCGCCAGCTGGAGTTTGCTAAAAAGATGCTCAGGAA
  GAAGGAACTTGATGAGGACATCCAAGCCATCCACAGCGCGAGACAGGGATCTGGGAGTCCGGCACACGCA
  AGGACGGCGGAGTCAGAGGTCCTGAAGACCCAGCTTGAGGAAGAGAGGAAGCGGGTGGCTGAGGTGCAGC
  GTGACCTGGAGGAGCAGAGGCAGCGGCTGCTGCAGCTGAGGACCCAGCAGCCTGTGGCGCGGCTGGAGGA
  GAAGGAAGTGGTGGAATTCTACCGGGACCCCCAGCTGGAGAGTAACCTGTCTCAGGCAGCATCCCGGGTG
  GAGGAAGAGGGCAAGAGGCGGGCCCGCCTGCAGGCCGAGCTGGAGGCGGTGGCCCAGAAGGTTGTCCACT
  TGGAGGGCAAGAGGAAGACCATGCAACCTCACCTGCTGACCAAAGAGGTCACCCAAATCGAGAGGGACCC
  TGGCCTGGACAGCCAGGTTACCCAGCTCCACAGTGAGATGCAACGGTTGCGCGGGGAGAACGGCGTCCTC
  ACAGCCAGGCTGGAAGAGCTGAAGGACGAGCTGCTAGCCCTGGAGCAGAAGGAGATGAACGTGAAGGAGA
  AGGTCGTGGTGAAGGAGGTGGTGAAGGTGGAGAAGGATCTAGAAATGGTTAAGGCGGCCCAGACTCTGAG
  GCTCCAGATCGAGGAAGATGCTGCACGGAGGAAGGGGGCGAAGGAGACCGTTGCCAAGATACAGGCTCGC
  ATCAAAGACCTGGAGCAGGCGATCAGCTCAGTGGAGCCCAAGGTCATTGTGAAGGAGGTGAAGAAGGTAG
  AGCAGGACCCGGGCCTTCTCAAGGAGGCTTCCAGGCTGAGAAGCCTCCTGGAGGAGGAGAAGAACAACAA
  CGTCGCGTTGGCCAGGGAACTGCAGGAGCTGCAGGAGAAATACAGGGTGGTGGAAAAGCAGAAGCCCAAG
  GTGCAGCTGCAGGAGCGAGTCAGCGAGATCTTCCAGGTGCTCCCGGAGACAGAGCAGGAGATCCGGAGGC
  TCCGGGCCCAGCTGCAGGAGACAGGCAGCAAGAAGAGCGGCGTGGAGCAGGAGGTAGAGAAGCTGCTGCC
  TGAGCTGGAGGTCCTGCGGGCTCAGAAGCCCGTGGTGGAATATAAGGAGGTGACTCAGGAGGTGGTGAGG
  CACGAGAAGAACCCAGAGGTGCTGCGGGAGATCGACCGTCTCAAGGCTCAGCTCAACGAACTGGTCAACA
  CCAACGGGCGCTCTCAGGAGCAGCTCATCCGGCTCCAGGGCGAACGCGATGAGTGGAAGCGGGAAAGGTC
  CAAGGTGGAGACCAAGATGGTGAGCAAAGAAGTCGTGCGGCACGAGAAGGATCCGGTGCTGGAGAAGGAG
  GCCGAGAGGCTCCGGCAGGAGGTGCGGGAGGCTGTCCAGAGGAGGCGAGCCACGGAGGATGCTGTGTACG
  AGCTGCAGAACAAGTTACTGCTGCTGGAGAGGAGGCGGCCCGAGGAGCAGATAGTGGTGCAGGAGGTGGT
  GGTCACCCAGAAGGACCCGAAGCTGCGCGAGGAGCACAGCCGGCTGAGCCGCAGCTTGGATGAGGAGGTG
  GGGCGCCGGCGGCAGCTGGAGCTGGAGGTGCGGCAGCTGGGCGCCAGAGTTGAGGAGGAGGAGGCCAGGC
  TCAGCTTCGAGGAGGATCGCAGCAAGAAGCTGGCCGCCGAGAGGGAGCTGCGGCAACTGACCCTGAAGAT
  CCAGGAGCTGGAGAAGCGGCCCCCTGCCCTGCAGGAAAAGATCATCATGGAGGAAGTGGTCAAGTTAGAG
  AAAGATCCTGACCTGGAGAGGTCTACAGAAGCTCTGCGGCGCGAGCTGGATCAGGAGAAGAACCGCGTGA
  CCGAGCTGCACCGGGAGTGCCAGGGTCTGCAGGTTCAGGTCGACCTCCTCCAGAAAACTAAATCACAGGA
  GAAGACCATATACAAGGAAGTGATCAGGGTCGAGAAGGACCCGGTGCTGGAGGGCGAGAGGGCCCGAGTG
  TGGGAGATCCTTAACCGGGAGCGCGCAGCCCGCAAGGGCCGGGAGGAGGATGTCCGGAGCTTGCAGGAGC
  GGATCGACCGAGCCGAGGCTCTGAGGAGGTCCTGGTCCCGCGAGGAAGCTGAGCTCCAGAGGGCCCGGGA
  CCAGGCAAGCCAGGACTGCGGGCGGCTGCAGAGACAGCTGCGGGAGCTGGAGCAGCAGAAACAGCAGAAA
  GCCCGGCAGCTACAGGAGGAGGGGAGGCTGCTTAGCCAGAAGACGGAGAGCGAGCGCCAGAAGGCCGCCC
  AGCGGAGCCAGGCGGTCACGCAGCTCGAGGCTGCCATTCTCCAGGAAAAGGACAAGATCTACGAGAAAGA
  GAGGACCCTCAGGGACCTGCACACCAAGGTGAGCCGGGAGGAGCTCAACCAGGAGACCCAGACCCGGGAG
  ACCAACCTCTCCACCAAGATCTGCATCTTGGAACCGGAGACCGGCAATGACATGTCTCCTTACGAGGCCT
  ACAAGAGGGGTGTCATCGACAGAGGCCAGTATCTCCAGCTGCAGGAGCTGGAGTGTGACTGGGAAGAAGT
  CACAACCTCCAGCCCCTGCGGGGAGGAGTCTGTGCTCCTGGACCGCAAGAGCGGGAAGCAATACTCCATC
  GAGGCCGCCCTCCGCTGCCGCCGCATTTCCAAAGAAGAGTACCACAGGTACAAAGATGGCCGCCTCCCCA
  TCTCCGAGTTTGCCCTGCTTGTGGCAGGGGAGACCAAGCCGAGTTCTTCACTCTCCATTGGGTCCATCAT
  CTCCAAGTCTCCAGTCTGCTCCCCAGGCCCCCAGAGTACCGGCTTCTTCTCCCCAGGCCTCTCTTTTGGG
  CTCACTGAGGATAGCTTCCCCATCGCGGGGATCTACGATACAACCACAGACAACAAGTGTAGCATCAAGG
  CAGCTGTGGCCAAGAACATGCTGGATCCTATCACTGGGCAGAAGCTGTTGGAGGCCCAGGCAGCCACGGG
  AGGCATTGTTGACCTTCTTAGCCGAGAACGGTACTCCGTACACAAGGCGGTGGAGCGGGGACTCATTGAG
  AACACCTCCACTCAACGGCTGCTCAATGCTCAGAAGGCCTTCACCGGCATCGAGGACCCTGTCACCAGGA
  AGAGGCTGTCGGTGGGTGAGGCCATCCAGAAGGGCTGGATGCCCCAGGAAAGCGTGCTTCCCCACCTGCT
  GGTACAGCACCTGACTGGGGGCCTCATCGACCCCAAGAGGACGGGACGCATCCCCGTCCCTCAGGCTGTG
  CTCTGCGGGATGATCAGCGAAGACCTGGGCCAGCTCCTGCAAGATGAATCCGGCTACGAGAAGGATCTGA
  CAGACCCCATCACCAAGGAGAGGCTGAGCTACAAGGAGGCCATGGGGCGCTGCCGCAAAGATCCTCTAAG
  TGGCCTACTACTGCTCCCCGCCATGCTAGAAGGCTATCGCTGCTACCGCGCGGCCTCCCCCACCCTGCCA
  AGATCCTGTGTGCGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025276
• Insert Size: 6108 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025276.3, NP_079552.3
• RefSeq Size: 6398 bp
• RefSeq ORF: 6108 bp
• Locus ID: 14027
• UniProt ID: Q9D952
• Cytogenetics: 11 E2
• Gene Summary: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.[UniProtKB/Swiss-Prot Function]