Dlg5 (NM_001163513) Mouse Untagged Clone

CAT#: MC225045

Dlg5 (untagged) - Mouse discs, large homolog 5 (Drosophila) (Dlg5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlg5
• Synonyms: 4933429D20Rik; mKIAA0583; T25557
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225045 representing NM_001163513
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCAGCGCCGGGAGCTGCTCGCCCAGTGTCAGCAGAGCCTGGCCCAGGCCATGACCGAGGTGG
  AGGCCGTGCTTGGGCTGCTGGAGGCAGCAGGAGCGCTGAGCCCCGGCGAGCGGCGGCAGCTGGACGAGGA
  GGCGGGAGGCGCCAAGGCGGAGCTGCTGCTCCAGCTCCTCCTGGCCAAGGAGCAGGACCACTTCCAGGAC
  CTGCGCGCCGCCCTGGAGAAGACTCAGCCTCACCTGCTGCCTATTCTCTACCTGAACGGCGTCGTCGGGC
  CGCCGCAGTCTACAGAGGGCGCGGGGTCCACCTACAGCGTCCTGTCCATCATGCCCTCAGACTCGGAGAG
  CAGCAGCTCCCTCAGCAGTGTGGGGACTACTGGGAAGGCACCGTCCCCACCACCCCTCCTCACCGAGCAG
  CAGGCCAATGACACGGTGGAGAACCTCTCCATTCAACTAAGGCTGATGACCCGTGAGAGGAATGAGCTAC
  GCAAGCGCCTGGCCTTCGCCACCCATGGAGCCACCTTTGACAAAAGGCCCTACCACAGGCTGAATCCTGA
  TTATGAGAGGCTAAAGATCCAGTGTGTGCGGGCCATGTCGGACCTGCAGAGCTTACAGAACCAGCACACC
  AACGCCTTGAAGAGGTGCGAGGAGGTGGCCAAGGAGACAGACTTCTATCACACCCTCCACAGCCGGCTCC
  TGAGTGACCAGACGCAGCTGAAGGACGATGTGGACATGCTGAGACGGGAGAACGGGAAGCTGCGGAGGGA
  GCGCAATCTGCTGCAGCAGTCCTGGGAGGACATGAAGCGGCTCCGGGAAGAGGACCAGAAGGAGATAGGT
  GACCTCCGGGCCCAGCAGCAGCAGGTGCTGAAGCACAATGGCTCATCAGAGATCCTCAACAAGCTGTACG
  ACACGGCCATGGACAAGCTGGAGGTAGTCAAGAAGGACTACGATGCCCTGCGGAAGAGATACAGCGAGAA
  GGTTGCCATGCACAACTCGGACCTGAGCCGCCTGGAGCAGCTGGGGGAGGAGAACCAGCGTCTGCAGAAG
  CAGACGGAGATGCTGACCCAGCAGAGGGACACGGCCATCCAGTTACAGCACCAGTGCGCACTCTCCCTGA
  GGAGGTTTGAGACGATCCACCATGAGCTGAGCAAGGCCACAGCCCAGAACAAGGACCTGCAGTGGGAGAT
  GGAGCTGCTGCAATCCGAGCTCACGGAGCTGAGGAGCAAGCAGGTGAAAACCGCCAAGGAGTCAGAGAAA
  TACAAGGAGGAGCGCGATGCTGTGTACAGCGAGTACAAGCTCATCATGAGCGAACGCGACCAGGTCATCT
  CCGAGCTGGACAAGCTACAGACAGAGGTAGAGCTGGCCGAGTCCAAGCTCAAAAGCAGCACATCCGAGAA
  GAAGGCAGCCAGCGAGGAGATGGAGGCCCTGCGGCAGATCAAAGACACAGTGACCATGGATGCTGGGAGA
  GCCAACAAAGAGGTGGAAATCCTCCGAAAACAGTGTAAGGCCTTGTGCCAGGAGCTGAAGGAAGCCCTCC
  AGGAAGCAGACGTGGCCAAGTGCCGGCGGGACTGGGCCTTTCAGGAGCGGGACAAGATTGTGGCAGAGCG
  TGACAGCATCAGGACCCTGTGTGACAACCTGAGGCGGGAGCGAGATCGAGCTGTGAGTGAGCTGGCTGAA
  GCCCTTCGCAGCCTGGATGACACTCGGAAGCAGAAGAATGACGTCAGCCGGGAGCTCAAGGAGCTCAAGG
  AGCAGATGGAATGTCAGCTGGAGAAGGAGGCCCGATTCCGGCAGCTGATGGCCCACAGTTCGCACGACTC
  AGCCATCGACACGGATTCCATGGAGTGGGAGACAGAAGTTGTGGAATTTGAGAGAGAGACGGAGGATATT
  GACTTGAAAGCACTTGGGTTTGACATGGCAGAAGGCGTGAATGAGCCTTGTTTCCCAGGGGATTGCGGCA
  TATTTGTTACTAAAGTGGATAAAGGAAGCATTGCTGATGGTCGCTTAAGGGTCAACGACTGGCTGCTGAG
  AATCAACGATGTAGACCTCATCAACAAGGACAAGAAACAGGCCATCAAGGCACTCCTCAACGGAGAGGGA
  GCCATCAACATGGTTGTGAGGCGGAGGAAGTCCCTGGGTGGGAAGGTCGTCACACCTCTGCACATCAACC
  TCAGTGGACAGAAAGACAGTGGCATCAGTTTGGAGAATGGAGTGTATGCTGCCGCAGTGGTGCCTGGAAG
  CCCGGCTGCCAAAGAGGGGTCCCTCGCCGTGGGAGACAGGATTGTTGCGATCAACGGTATTGCACTGGAC
  AATAAGTCTCTAAATGAATGTGAATCTCTGCTGCGCAGCTGTCAAGACTCCTTGACCCTCTCCCTCCTGA
  AGGTGTTTCCCCAGAGCTCCTCATGGAGTGGCCAGAACATCTTTGAAAATATCAAGGACTCAGATAGGAT
  GCTGAGTTGCCGAGCCCACGGCCCAGAGGTCCAGGCGCATAACAAACGGAACTTGTTACAGCACAATAAT
  TCCACGCAGACAGACATTTTCTACACGGATCGGCTGGAGGACAGGAAGGAGCTGGGACATTCTGGAGGCA
  GCAGTTCTTTTCTCCACAAGCCCTTCTCTGGATCCTCGTCTCCAGTCTCCCCTCAGGCCTGTCCTAGCAC
  CTCTGAGCGTAGTCTGAACTCATTCCGCTCTGATACCTCTGCAGAACGTGGCTATGGGCTGGTGGATATG
  CGGAGCCAGCGGCCACTGCTGTCTTTTGAGACTGAGGTGGGCCCCTGTGGGGCAGTAGAGGTTCCCCTGG
  ACAAGATAGATCCAGAGGGCTCCAATAGTGGTGGGACCTGGCCCAAGGCTGTGCTTGGCTCCACATCAGG
  GCCTGAGAAACTCTCTGTGTATAAGAAACCCAAACAAAGGAAGTCCATCTTTGACCCAAATACTTTCAAG
  CGCCCACAGACACCCCCCAAAATAGACTACCTGCTTCCAGGCCCTGGGCTTACTCATTCCCCCCAGCCCT
  CCAAGAGGGTAGGATCTCTGACACCCCCAAAGCCCCCCAGGAGAAGTGACTCTATCAAGTTCCAACACAG
  GCTGGAGACCAGTTCTGAGTCAGAAGCCACTTTGGTGGGCAGCTCACCTTCCACCAGCCCTCCCAGTGCC
  CCACCCCCCAGCATGGATCCCAGTGAGCCCACGCATGCTTCACCTCCTCGGAAGGCCAGGGTCCGCATTG
  CCTCCAGCTACCACTCTGAAGGGGACGGAGACACCTCCTACCTGCCCGCCAAGAAGCCTTGTGATGAAGA
  CCTCACCTCCCAGAAAGTCGATGAGCTGGGGCAGAAACGGCGCCGGCCAAAGTCTGCCCCAAGTTTTCGG
  CCCAAGATCTCTCCAGTGGTGATCCCTGCTCAGTGCCTGGAGGAACAGGAGTGTGTTCCAGCCATCGGAG
  AGCTCTCCCCAGAGGGTCAGGAATGGTCCCCGTACTCACCAGGGCATGCTAGCCGGCATGGCAACCCCCT
  ACTCTACCCCAACCGGCCATCTGTGGGCACCGTTCCCCGGAGTATGACCCCCGGCACCACTGTGGGCTCC
  ATTCTGAGGAACCCCATTTATACTGTGCGCAGCCACAGGGTGCTTCCCTGCGGCTCACCACCCGTGCCCC
  GTGATGCTGGCTCCCAGAGCTTGTCTCCCAGTGTCCAGCATCAGGGACGCCTCAGCCTGGACCTGAGCCA
  CAGGGCCTGCAGTGACTACTCTGAGATGAGAGCCTCCCAGGGTTCCAACTCTCTGCCCTCCAGTGCCCGC
  CTTGGCTCTTCCAGTAACCTGCAGTTCAAGGCTGAGCGGATTAAGATCCCGCTAACACCAAGATATCCCC
  GGTCTGTCATGGGCTCTGACAGAGGCTCACTGTCACATTCTGAATGTAGCACTCCTCCCCGGTCCCCGCT
  GAATATCGACACCCTGTCCTCCTGTAGCCAGCCTCAGACCACAGCCTCCACTTTACCCAGGATTGCGGTC
  AACCCCTCTTCCCATGGGGAGCGGAGGAAGGACAGGCCTTTTGTGGAAGAGCCACGCCACGTGAAGGTGC
  AGAAGGGTTCAGAGCCTCTGGGTATCTCCATCGTGAGTGGAGAAAAGGGTGGTGTCTATGTCTCCAAGGT
  GACCCTGGGGAGCATCGCTCATCAAGCTGGACTTGAGTATGGGGACCAGTTACTTGAGTTCAATGGCATA
  AACCTGAGAAGTGCCACCGAGCAGCAGGCCCGGCTCATCATCGGACAGCAGTGTGACACCATCACCATCC
  TGGCCCAGTACAACCCACACATCCACCAGCTCAACAGCCACTCCCGCTCCAGCTCTCATCTGGACCCAGC
  TGCTACCCCTCACTCTACTCTCCAGGGCAGCAGTGCTGGGACCCCAGAACATCCCTCTGTCATCGACCCA
  CTGATGGAACAGGATGAAGGTCCTGGCACCCCCCCAGCCAAGCAAAGCGCCTCCAGCACCAGGAGTGTGG
  GAGATACCACCAAGAAGACCCCTGATCCCCGCATTGTTTTCATCAAGAAATCCCAGCTGGATCTGGGGGT
  GCACTTGTGTGGTGGGAACCTGCACGGAGTGTTTGTGGCCGAAGTGGAAGATGACAGTCCTGCCAAGGGT
  CCTGATGGCCTCGTCCCAGGAGACCTCATCCTTGAGTATGGCAGCCTGGATATGCGCAGCAGGACAGTGG
  AGGACGTCTATGTGGAGATGCTGAAGCCTAAGGATAGCCTTCGCCTGAAGGTGCAGTACCGCCATGAGGA
  GTTCACCAGGGTCAAGGGCCTGCCCGGAGACAGCTTCTACATCAGGGCCCTATATGACCGTCTGGCAGAG
  GTGGAGCCTGAACTGAGTTTTAAGAAAGATGACATCCTGTACGTGGATGATACGTTGCCCCAGGGCGTGT
  TTGGGTCCTGGATGGCCTGGCAGCTGGATGAGAATGCCCAGAAGATCCAGCGTGGGCAGATTCCCAGCAA
  ATACGTGATGGACCAAGAGTTCTCCCGGAGGCTCAGCATGTCTGAGGTCAAAGATGACAACACAGCCAAG
  ACGCTGTCAGCAGCTGCGCGCCGTTCCTTCTTCCGAAGGAAACATAAACATAAACGCAGTGGGTCCAAAG
  ACGGCAAGGACCTGCTCGCCCTGGACACCTTTTCCAACGACTCCATTCCTCTCTTTGAAGACTCAGTGAG
  CCTGGCCTATCAGCGGGTCCAGAAAGTGGACTGTACCTCTCTTCGGCCTGTTCTCCTCCTGGGACCTTTA
  CTGGATGTGGTGAAAGAGATGCTGGTGAATGAGGCACCTGGCAAGTTCTGCAGATGCCCACTTGAGGTGA
  TGAAGGCTTCCCAGCAAGCCATTGAGCGGGGTGTCAAAGACTGCCTGTTTGTGGACTACAAGCGGAGGAG
  TGGCCATTTTGATGTTACTACTGTGGCTTCAATAAAGGAGATCACAGAAAAGAACCGGCACTGTCTTCTG
  GACATCGCCCCGCATGCCATCGAGAGGCTGCACCACATGCACATCTACCCCATTGTCATCTTCATCCGCT
  ACAAGAGCGCCAAGCACATCAAGGAACAGAGAGACCCTGTCTACCTGAGGGACAAGGTGACACAGAGGCA
  TTCCAAAGAGCAATTCGAAACAGCACAGAAGATCGATCAGGAGTACAGCAGGTACTTCACAGGGGTTGTC
  CAGGGTGGAGCCCTGTCCAGCATTTGCACTCAGATCCTGGCCATGGTCAGTCAAGAACAAAGTAAAGTCC
  TGTGGATCCCCGCTTGCCCACCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163513
• Insert Size: 5766 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163513.1, NP_001156985.1
• RefSeq Size: 7878 bp
• RefSeq ORF: 5766 bp
• Locus ID: 71228
• UniProt ID: E9Q9R9
• Cytogenetics: 14 A3
• Gene Summary: Acts as a regulator of the Hippo signaling pathway. Negatively regulates the Hippo signaling pathway by mediating the interaction of MARK3 with STK3/4, bringing them together to promote MARK3-dependent hyperphosphorylation and inactivation of STK3 kinase activity toward LATS1 (PubMed:28087714). Positively regulates the Hippo signaling by mediating the interaction of SCRIB with STK4/MST1 and LATS1 which is important for the activation of the Hippo signaling pathway. Involved in regulating cell proliferation, maintenance of epithelial polarity, epithelial-mesenchymal transition (EMT), cell migration and invasion (By similarity). Plays an important role in dendritic spine formation and synaptogenesis in cortical neurons; regulates synaptogenesis by enhancing the cell surface localization of N-cadherin (PubMed:25232112). Acts as a positive regulator of hedgehog (Hh) signaling pathway. Plays a critical role in the early point of the SMO activity cycle by interacting with SMO at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (PubMed:25644602).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).