Dock2 (NM_033374) Mouse Untagged Clone

CAT#: MC224955

Dock2 (untagged) - Mouse dedicator of cyto-kinesis 2 (Dock2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dock2
• Synonyms: AI662014; AW122239; CED-5; Hch; MBC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224955 representing NM_033374
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCTGGCGCAAAACTGACAAGGAGCGTCACGGAGTGGCTATCTACAACTTCCAAGGCAGTGAAG
  CCCAGCATCTCACGCTACAGATTGGCGATGTGGTACGAATACAGGAGACCTGTGGAGACTGGTACAGAGG
  GTACCTCATAAAGCATAAACTGTCACAGGGCATTTTCCCTACATCCTTTATCCATCTCAAGGAAGTGACA
  GTGGAGAAGAGAAGGAACATAGAGAACATCATTCCTGCAGAAATCCCTCTGGCACAAGAAGTGACAACCA
  CGCTCTGGGAGTGGGGAAGCATCTGGAAGCAGCTCTATGTGGCCAGCAAAAAGGAACGCTTCCTCCAAGT
  GCAGTCCATGATGTACGACCTGATGGAATGGCGCTCTCAGCTCCTCTCAGGAACGCTACCCAAGGATGAG
  CTGAAGGAACTGAAGCAGAAAGTCACATCGAAGATCGACTATGGCAACAAAATCCTTGAGCTCGATCTCA
  TTGTCAGAGATGAAGATGGAAACATACTGGACCCTGATAAGACCAGCGTCATCAGCTTGTTCCATGCTCA
  CGAGGAGGCAACTTACAAAATCACAGAGCGCATCAAAGAAGAAATGTCAAAAGACCAGCCAGATTATGGG
  GTTTATTCTCGGATCTCCTCATCCCCCACCCACAGCCTCTACGTCTTTGTGAGAAACTTTGTGTGCCGAA
  TTGGGGAGGATGCTGAGCTCTTCATGTCTCTCTATGACCCTCACAAGCAAACGGTCATCAGTGAGAACTA
  TCTGGTGCGATGGGGCAGCAAAGGATTCCCAAAGGAAATTGAGATGCTCAATAACCTGAAGGTAGTCTTC
  ACGGATCTTGGAAATAAAGACCTCAACAGGGATAAGATTTTCTTGATCTGTCAAATAGTCCGAATTGGGA
  AGATGGATCTTAAGGACATTAATGCCAAGAAGTGCACCCAGGGGCTGAGGAGACCTTTTGGGGTGGCAGT
  TATGGACATCACCGACATCATCAAGGGCAAGGCAGAGAGCGACGAAGAGAAGCAACATTTTATTCCATTT
  CACCCGGTCTCAGCTGAGAACGATTTCCTTCACAGCCTTCTGGGCAAAGTCATAGCTTCCAAGGGAGACA
  GTGGAGGGCAAGGCCTTTGGGTGACCATGAAGATGCTGGTGGGGGACATCATTCAGATCCGTAAAGACTA
  CCCACACTTGGTGGACAGGACCACTGTGGTAGCCCGAAAGCTGGGCTTCCCAGAGATCATCATGCCAGGG
  GATGTCAGGAACGACATCTACATCACTCTCTTACAAGGTGACTTTGACAAGTACACCAAGACCACACAGC
  GGAACGTGGAAGTGATCATGTGTGTGTGCACAGAGGATGGCAAAGTGCTACCTAATGCAATTTGTGTGGG
  AGCGGGGGATAAGGCCATGAACGAGTACCACTCAGTCGTCTACTACCAAGTCAAACAGCCCCGATGGATG
  GAAACAGTCAAGGTGGCTGTCCCTATTGAAGATATGCAGAGGATCCATTTGAGGTTCATGTTTCGACACC
  GCTCATCACTAGAATCTAAAGATAAAGGAGAAAAGAACTTTGCCATGTCCTACGTGAAACTCATGAAAGA
  AGATGGAACCACTCTGCATGATGGATACCACGAGTTGGTGGTTCTCAAGGGAGACAGCAAGAAAATGGAA
  GATGCCAGCGCTTACCTGACACTTCCTTCTTATCGACACCCCGTGGAAAACAAGGGAGCTACGCTGAGCC
  GGAGCTCCAGCAGTGTTGGGGGCCTTTCTGTCAGCTCCCGGGATGTGTTCTCCATTTCCACGCTAGTGTG
  CTCCACAAAGCTGACCCAGAATGTGGGTTTGCTTGGCTTGCTGAAGTGGCGTATGAAGCCACAACTGCTC
  CAGGAGAATCTAGAGAAGTTGAAGATTGTGGATGGCGAGGAAGTAGTGAAGTTCCTCCAGGACACTCTGG
  ATGCCCTCTTCAACATCATGATGGAACACTCTCAGAGTAACGAGTATGACATCCTCGTCTTTGATGCTTT
  GATCTATATAATAGGACTCATTGCAGACCGGAAATTCCAGCATTTTAACACCGTATTGGAGGCTTACATC
  CAACAGCATTTCAGTGCTACCTTGGCCTACAAGAAACTGATGACGGTGCTGAAGACTTACTTGGATACCT
  CCAGCAGGGGGGAGCAGTGCGAGCCCATCCTCAGAACGCTCAAAGCCTTAGAATACGTGTTCAAGTTCAT
  TGTTCGGTCGAGGACATTATTCTCACAGCTTTACGAAGGCAAAGAGCAGATGGAGTTTGAAGAATCCATG
  AGACGGCTCTTCGAATCCATCAACAACCTGATGAAAAGTCAGTACAAGACCACCATCCTATTGCAGGTGG
  CGGCTTTGAAATACATCCCTTCAGTCCTTCACGATGTGGAAACAGTCTTTGATGCCAAGTTGCTCAGCCA
  GCTACTGTATGAGTTCTACACCTGCATCCCTCCCGTGAAGCTGCAGAAGCAGAAAGTTCAGTCCATGAAT
  GAGATCGTGCAGAGCAACCTCTTCAAAAAGCAAGAATGCCGGGACATTCTGCTTCCTGTCATCACCAAAG
  AGCTGAAGGAGCTGCTGGAGCAGAGGGACGATGGGCAGCACCAGGCTGAGAAGAAGCACTGTGTGGAACT
  TCTCAACAGCATCTTGGAGGTCCTCAGCTGTCAGGATGCGGCCTTCACTTATGACCACATCCAAGAGATC
  ATGGTCCAGCTGCTTCGCACAGTGAACCGGACAGTCATCACGATGGGCCGAGATCACGCTTTGATTAGTC
  ACTTTGTGGCGTGTATGACAGCTATCTTAGACCAGATGGGCGACCAACACTATTCATTTTACATTGAGAC
  CTTCCAGACCAGCTCAGACCTTGTGGACTTCTTGATGGAGACATTCATCATGTTCAAGGACCTCATTGGG
  AAGAATGTGTATCCTGGGGACTGGATGGCCATGAGCATGGTCCAGAACCGGGTCTTCCTGAGAGCCATCA
  ACAAGTTTGCAGAAACCATGAACCAGAAGTTTCTGGAACACACAAGCTTTGAGTTCCAGCTGTGGAACAA
  CTATTTTCATCTGGCAGTTGCCTTTATCACTCAGGACTCTCTGCAGCTGGAGCAGTTCACACACGCTAAG
  TACAACAAAATCCTGAATAAGTACGGGGACATGAGACGGCTCATAGGATTCTCCATCCGTGATATGTGGT
  ACAAGCTTGGCCAGAACAAAATCTGCTTCATCCCGGGCATGGTGGGACCTATATTAGAGATGACACTTAT
  CCCTGAGGCTGAGCTCAGGAAAGCCACCATCCCAATCTTCTTCGACATGATGCTGTGTGAATATCAAAGG
  ACTGGGGCTTTCAAAAAGTTTGAAAATGAAATCATCCTGAAGCTGGACCATGAGGTGGAAGGAGGCCGGG
  GGGATGAGCAGTACATGCAACTGCTGGAGTCCATCCTGATGGAGTGCACAGCGGAACACCCAACCATTGC
  CAAGTCGGTGGAGAACTTTGTGAGCTTGGTCAAAGGGCTGCTGGAGAAGTTGCTAGATTACCGGGGCGTG
  ATGACAGATGAGAGCAAAGACAACCGTATGAGCTGCACAGTGAACTTGCTGAATTTCTACAAAGATAACA
  ACCGGGAAGAGATGTACATAAGATACCTGTATAAACTGCGTGACCTTCACTTGGACTGTGAAAACTACAC
  AGAGGCTGCCTACACACTCCTCCTCCACACCTGGCTTCTCAAGTGGTCAGATGAGCAGTGTGCATCCCAG
  GTCATGCAGACAGGCCAGCAGCACCCTCAGACCCACCGGCAGCTGAAGGAGACACTCTATGAGACCATTA
  TAGGCTACTTTGACAAAGGAAAGATGTGGGAAGAGGCCATCAGCCTGTGCAAGGAACTGGCGGAACAATA
  TGAGATGGAGATCTTTGACTACGAGCTACTCAGCCAGAACCTGACCCAGCAGGCCAAATTCTATGAAAAC
  ATCATGAAAATCCTCAGAACCAAACCAGACTACTTTGCTGTTGGATACTATGGCCAGGGATTCCCCTCCT
  TTCTGCGGAACAAAGTGTTCATCTACCGAGGCAAGGAATATGAGCGAAGAGAAGACTTCCAGATGCAGCT
  TTTGAGCCAGTTCCCCAACGCAGAAAAGATGAACACAACTTCTGCCCCAGGAGACGATGTGAGGAATGCC
  CCAGGCCAGTACATCCAGTGTTTCACTGTGCAGCCTGTTCTGGATGAACACCCCAGGTTCAAGAACAAAC
  CGGTGCCTGACCAGATCATAAACTTTTACAAGTCTAATTATGTGCAAAAGTTCCACTACTCCAGGCCTGT
  GCGCAGGGGCAAGGTAGACCCAGAGAACGAGTTTGCTTCCATGTGGATCGAGAGGACTTCCTTCCTGACG
  GCCTACAAGCTGCCTGGCATCCTGCGCTGGTTTGAGGTAGTTCACATGTCTCAGACCACAATTAGTCCTC
  TGGAGAATGCCATCGAGACTATGTCCACAGTCAACGAGAAGATCCTGATGATGATAAACCAGTACCAGAG
  TGATGAAAGCCTCCCCATCAACCCTCTCTCCATGCTCCTCAATGGGATCGTGGACCCTGCTGTCATGGGA
  GGCTTCGCCAAGTATGAGAAGGCCTTCTTCACTGAAGAGTATAGCAGGGAGCACCCGGAGGACCAGGACA
  AGCTGAGCCATCTCAAGGACCTGATTGCATGGCAGATCCCTTTCCTGGGAGCTGGGATTAAGATCCACGA
  GAAAAGGGTGTCAGACAACCTGCGCCCCTTCCATGACCGGATGGAGGAGTGCTTCAAGAACCTGAAAATG
  AAGGTGGAAAAGGAGTATGGTGTCCGAGAGATGCCTGACTTCGAAGACAGGAGAGTGGGCCGCCCCAGGT
  CCATGCTGCGTTCCTACAGGCAGATGTCCGTCATCTCTCTGGCCTCCATGCATTCTGACTGCAGCACTCC
  CAGCAAAGTCCCCGCAGAAAGTTTTGACCTAGAGTCAGCCCCACCCAAGACTCCCAAAGTGGAGGAGGAG
  CCCATCTCCCCAGGGAGCACTCTGCCAGAGGTCAAGCTGCGGCGGTCCAAGAAAAGGACCAAGAGGAGCA
  GCGTGGTGTTTGCAGACGAGAAGGCAGCAACGGAGTCAGACCTGAAGCGGCTTTCTAGAAAGCAAGAGTT
  CATGAGTGACACCAACCTCTCAGAGCATGCAGCCATCCCTGCCAGGGTGTCTATCCTCTCTCAGATGAGT
  TTTGCCAGCCAGTCCATGCCCACCATCCCAGCCCTCACACTCTCCGTGGCAGGCGTGCCTGGGTTGGATG
  AGGCCAACACATCTCCCCGCCTGAGTCAGACCTTCTTCCAAGTCTCAGATGGTGACAAGAAGACCCTGAA
  AAAGAAGAAAGTCAATCAATTCTTCAAGACCATGCTGGCTAGCAAGTCGAGTGAAGAAAGCAAGCAGATC
  CCAGACTTCCTGTCCACCAACATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033374
• Insert Size: 5487 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033374.3, NP_203538.2
• RefSeq Size: 6431 bp
• RefSeq ORF: 5487 bp
• Locus ID: 94176
• UniProt ID: Q8C3J5
• Cytogenetics: 11 A4
• Gene Summary: Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.[UniProtKB/Swiss-Prot Function]