Fam208a (NM_001114879) Mouse Untagged Clone

CAT#: MC224781

D14Abb1e (untagged) - Mouse DNA segment, Chr 14, Abbott 1 expressed (D14Abb1e), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam208a
• Synonyms: 4732418E12; 4933409E02Rik; D14Abb1e; MommeD6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224781 representing NM_001114879
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTGCCGCGGAGACGGAGGCCCCTTCGACGGACGCGAGCTGGAAAAGCCGCGGCGGCGGCGGCG
  GAGACGACGGGATGAAGCCAGCGCTTCCGGAGCTTGAGTCCTCCCTACAAAATGGCGGCGGCGACGGCGG
  CGGCGGCGCGGGGCCTGAGGAGACCGCGGCAGCTGAAGCCGCCCGGAGCTACGGCCACGAGCAGCCTCAG
  CAAACCTCCGAAGCGGCCGCGGCCGCTCTGCCCAAAGGCGCCGAAGAGCCGGAAAGGCCTTTTAGGCGGA
  GTTTTCAGATCCCCAGGAAGAGCAGAGAAAAGAAAGCTCTTTTCCAGCCATTAACTCCAGGGTCTCGAGA
  ATTTGAAGATGTTTTAAATATTCTCCATTCATCTTACCTTGAACCATCATCAGTAACATATTTTAACTAT
  AGACGAGCTTGCTTGATACATAATGAACTTTTGGAAAAAGAGTTTACAGAAAAGAGAAGAGAATTGAAGT
  TTGATGGTCGTTTAGATAAGGAACTTTCAGAATCTTATGCATTTCTCATGGTTGATCGGTACCAGGTTCA
  GAGCATATGTGAAAAAGGATTGCAGGTGGGCCAATCCAAAATAACAGTTCTTGGCAGTCCTTCCATGGGT
  ATCTATCTTTGTAGGTATGCAGATCTCTTACAGGCTAATCCTTTGGAGGCTGGGGCAGTAGGCGATGTTG
  TTATTTTTAAAATCATGAAGGGTAAAATAAAGAGTATTTATGACCCTCTCAGTGTAAAAAGCTTGGAATC
  CATGTTAAGTAAAAATGCTTTGGACCCTACCCCAAAACATGAATGTCATGTGTCTAAAAATGCCAGTAGA
  ATTACATCCCTCTTGGCATATAGAGCCTATGAGCTTACTCAGTATTATTTTTATGAGTATGGCTTTGATG
  AAGTAAGACGAAGACCAAGACATGTTTGTCCGTATGCAGTTGTGTCTTTTACTTACAAAGATGATGTACA
  AACTCCAAAGTTTTTGTCTCCATTGAGATCTAACAGCTTTAATGCAGATAGAAACATAGATAAGTTTAAC
  TATACCTTGTGGAAAGGACAACTTTTAAATAAAGGAAAGCTTCTCTGTTATATTTCTTTGAGGTCCGCCA
  ATCGTGCCTTTCTACCTGTCAAGCTTCCTGAGAAATTGGATGTTGAAACAGTTATGAGTATTGATTGTTT
  GAAACAGAAAATTCCTCCTTCATTCTTTTATAAAGACACATATGTGGGTCCCAATGAAGTTTTAAAGAAT
  GGAATGTATTGTAGCCTTTATGAAGTTGTAGAAAAGACAAGAATTGGAAGTAATATGGAATGTTTACTGC
  AAAAACTGGAGAAAGAAAAACTTGTTCTTGTTAAACCTTTAGGAGACCGAGGATACCTTTTTCTTCTTTC
  TCCTTTCCAGATGGTTTCTCCATATGAACATCAGACTGTCAAGTCACGAATCCTACATGCTTTATTTCTG
  TTTCAAGAACCTAGATGCCTAATTATCACACAAAAAGGTATAATGAATACAACACCACTGGAGAAGCCTG
  AGAACTTAGCAGATATATTAAAAATAACTCAGTTTTTACAGTTTTCTCTGATTCAATGTAGAAAAGAATT
  CAAAACTATAAACACTATAAATTTTCATTCTGTTGTTGAAAAGTATGTAAGTGAATTTTTTAAGCGAGGT
  TTTGGTTCGGGTAAGCGAGAGTTTTTTATGTTTTCATATGATTCACGATTAGATGATAGAAAATTCCTAT
  ACTCAGCACCAAGAAATAAATCCCATATTGATGACTGCTTACATACCTACATTTACCAGCCAGAAATGTA
  TCAGTTGTCTATTTTTAAATTAAAAGAGTTATTTGAGGAAAATTGGAGACGCCAGCAGTTTAGTCCACTG
  TCAGATTATGAAGGTCAAGAAGAAGAACTGAATGGTTCAAAAATGAAATTTGGAAAACGGAATAATTCAA
  GGGATGAAACTACTGAACCTGAACAGCAGAAGTCGTCTCATTCTTTGGATTATGATAAAGATAGAGTCAA
  AGAGTTGATTAATTTAATTCAGTGTACAAAAAAAAATGTGGGTGGAGATCCAGATCCAGAAGATACAAAA
  AGCAAAAATGTCTTGAAGAGGAAGCTTGAAGATCTACCTGAAAATATGAGAAAGTTTGCCAAAACAAGTA
  ATTCAACTGAAAGTTGCCATCTATATGAAGAATCTCCACAGTCCATTGGCTTACTTGGACAGGATCCTAA
  CTTGAGAGTGCAGCAGGAAGATTCCGGGAACACTGGGGACATCCACAAGCTTTATAATTGGCTATCGGAA
  GCACTAGCAAATGCACGACACTCAGACGGATTCCTGACCGAGACAGTCAACAAGGCTTTAGGATTGAGTA
  GTAGTGGTGCCTATGAAGAGCTGAAGCAAAAATGTGATTATGAGTTGAACTCTACTTTAGATAAAAAAGA
  ATCTGAGCAGCCTGCTTGTACAAAAATTGAAAATGTGCATTTTAAGGATGCTCAGAGCCCATTGCTAGAA
  GTGGATGCAGCATCTGTAAAGTATCCTCCTCTTCTGTCTAGCAGTGAGGATCCGAATTTAATTAATGTCA
  ATAATTTTGAAGGGTGCAGTCTCTGTCCCACGGTTTCTATTGAGCATGGATTCCTTAGACAACATTCTAA
  GTCAAATGATGATGAAGAGACAGAAATACATTGGAAATTGATTCCAATTACAGGAGGGAATGCAGGAAGC
  CCAGAAGACCAGCATGGGAAACATGGTGAGAAACAAACACCAGGAATGAAATCACCAGGAGAACAGCTGG
  TGTGCCTGCCACCAGTGGAGGCCTTTCCTAATGATCCCCGGGTCATCAATAGAGAAAGAAGCTGTGATTA
  CCAGTTCCCATCCTCTCCGTCTACAGACACTCTAAAAGGCACTACCGAGGAGGACACTGTAACAGCAGGT
  CAGGCGATGGCAGTGGAAGAGCAGTGTGTGCCAGCAGCAGAGCTTCCTAGAGTGAGCGAGATTACAGAAA
  ATACAGTGTTAGGAGAGTTCCATCTTTTCTCTAGGAAGGTAGAAGAGATTTTGAAGGAGAAGAATGTTTC
  ATATGTTAGTGCAATTTCCACACCTATCTTTTCAGCACAAGAGAAGATGAATCGCCTTTCTGAGTTCATA
  CATTCTAATACTTCTAAAGCTGGTGTTGAGGAATTTGTAGATGGTTTGCATGAAAAACTAAATACTGTTG
  TTATTACAGCATCAGCTAAGGGTGTGAGTTTGCCGCCAGCAGTTAGCGCTAATCATTCCCATGCTGCAGC
  AGCATTGGCTTCTCTGGGAAGGCGTGTTGTGTCAATTTCCTCAAGTGACTTCAGTGCTAAAGAACTTTTT
  GAGCCGCTCTGTTCTGAACATTTAAAAGATAACAACTCTAATGAACAGTATTCCTCTTCAGTGGAAGTAG
  AAATGAATCGGCCACACCATTGCAAAGAGTTAATGTTGACTTCTGATCACACTGTACCTGGTGATACAGT
  CCTGGAACCCACAGAAAAAGAAATAACAAAATCACCCAGTGATATAACCATTTCTGCACAACCAGCACTT
  TCAAATTTTATAAGCCAGTTAGAACCTGAAGTATTTAATAGTTTGGTTAAAATCATGAAAGACGTCCAGA
  AAAATACTGTGAAGTTTTACATTCACGAAGAAGAGGAGAGTGTGCTTTGCAAAGAAATAAAGGAGTATCT
  TACCAAATTAGGAAATACAGAGTGTCATCCGGATCAGTTTTTGGAGAGAAGATCAAACTTGGATAAACTA
  TTGATTATTATTCAAAATGAAGACATTGCAGGTTTCATTCACAAGGTGCCTGGCTTAGTGACTTTAAAGA
  AGCTCCCATGTGTTAGTTTTGCTGGTGTTGATAGCCTGGATGATGTTAAAAATCATACATACAACGAGTT
  ATTTGTATCTGGTGGCTTTATTGTGTCTGATGAATCAATTCTAAATCTAGAGGTTGTCACAATTGAGAGC
  CTTAAAATTTTTTTGACATTCCTTGAAGAACTTAGTACTCCAGAAGGGAAATGGCAATGGAAAATCCACT
  GTAAATTCCAGAAGAAACTAAAGGAACTGGGCAGAATGAACACGAAAGCTCTGAGTCTGCTGACTCTTCT
  GAATGTGTATCAGAAGAAACATCTGGTTGAGATTTTGTCTTATCACAGTTGTGATTCACAAACTCGAAAT
  GCTCCAGAAATGGACTGCCTTATTAGGCTTCAGGCTCAGAACATACAGCAGCGACACATAGTCTTTCTAA
  CAGAGAAGAATATCAAGATGGTTTCCAGTTATACAGACAATGGAATTGTCGTTGCAACTACTGAAGACTT
  TATGCAAAACTTTACAAGTCTTGTGGGCTATCACAACTCAGTTACAGAAGAAAGTCTTCCGCCGCTGCTT
  GGTGCTAATGAGAACCTTGAGTCCCAGTCAGCTCTTTTAGAAAACGATGAAAAGGATGAAGAGGATATGT
  CCCTGGATTCAGGGGATGAAATCTCACATATAGAAGTATTCAGCAATGTTCATTCAGAAATATTGGCGGG
  AGAAACCAAAGGATCAAGTGGAACAGATCAAAAAAAGAATATCCAAATTGAATTGCAGTCATCTCTTGAT
  GTGCAAAACAGTTTATTAGAAGATAAGACTTATTTAATTGATTGTGAAGCGAGCGCTCCTATTGATAGAG
  TATGCTCTGAAGGAGAGAGCAGCAATTCAGCAGAACAGGATGCGTATAGCGACTTTCAGGCTGACCAAAA
  TCAGTTACAAATGTCCCATCAGTGTAGTCATTTTAATGTTCTCACGCACCAGACGTTTCTGGGGACACCA
  TATGCCCTTTCATCAACTCGGTCTCAAGAAAATGAAAATTACTTTTTATCTGCTTACAAAAACTCGGGTA
  CAGAGAAATCTCCATTAAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001114879
• Insert Size: 4923 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114879.1, NP_001108351.1
• RefSeq Size: 7307 bp
• RefSeq ORF: 4923 bp
• Locus ID: 218850
• UniProt ID: Q69ZR9
• Cytogenetics: 14 A3
• Gene Summary: Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2. Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).