D930015E06Rik (BC062940) Mouse Untagged Clone

CAT#: MC224589

D930015E06Rik (untagged) - Mouse RIKEN cDNA D930015E06 gene (cDNA clone MGC:86126 IMAGE:6842400), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D930015E06Rik
• Synonyms: Kiaa0922; mKIAA0922
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC062940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAAGCATCCTTCAGAGTTATTTTCTTACCTACCGAAGAAGGGAGCATCGAAAGCTCTTTATTTA
  TTAATACCTCCTCACACGGAGTCTTCTCCTATCATGTATCTGGAGTGGGCACTCGCAGAGTCTCTACAGA
  AGGGTCTGCGGAGCAGCTGCCGAACGCATACTTTCTGCTTCCACAGGTCCAAAGTATTCAGCTTTCACAG
  ACACAGGCAGAGACCACTAATACCAGCCTGCTGCGGGTGCAACTGGAATGCAGTTTACATAATAAAGTGT
  GTCAGCAATTGAAGAGTTGCTCTCTGGGATCTGATGATGCGCTACACTTGGAGATGAACATCATTGTAGC
  AGTGGAAAACTCCTCCAAGCAACCTGAAGAGAACACTCAAGCCCTGCTAGATCACCTCTCTATTGTCTAT
  GTAGCTACTGATGAGTCTGACACCTCAGATGAATCTGCAGTAAATATGTATGTACTGCATTCAGGAAACA
  GCCTTATTTGGATACAGGATATACATCATTTCTCACAGAAAAATGTCCTGTCTCTGCAGTTTGAACCAGT
  ACTGCTCTCTACTTCCACGACAAACTTCACAAAAATCGCCTCGTTTACTTGCAAAGCAGGTACCTCGTGT
  GACAGTGGAATCATGGGCCTGAGGAAAAAAAAAGCTAGCCCAGCAATGCAAGCCTGCCTGTCCTCTCCTG
  TGGTGCAGGGGTATTTCAGAACGGATGCCTCTACAGCCCAGTTTCACATAGAGAGTCACGAGACTGCAAC
  AGGAGTCTGGTCAATATGGTACCGCAGCCATTTTGACCAGAGCATTGTATTAAAAGATGTGTTTGTTTCC
  AAGGAGACCAAGCACATTTTAAAGGTTCTGAGTTTCCGAGGCCCTTTATTTCTACCCCCAGGCTGTTGGA
  ATATATTTTCTCTGAAGCTTGCTGTTAAAGGCATTGTCTTAAACCTGTTCACCAATGTATTTCTGACTAC
  AAACACAGGTGCCATTTTTGCAATACCTCTGCAGATTTTCTCGGCCCCAACCAAGGAAGGGAGTCTGGGT
  TTTGAAGTGCTAGCACATTGTGGCATGCATTATTTCATGGGAAAGTCAAAAACAGAAAATCCTAATTGGG
  AAAGAAGCCTTTCTCTGGATCGGTCCACCTGGGACATGGATTCTGAACTTGCAAATAAGCTGTATGAAAG
  ATGGAAGAAATATAAAAGTGGCGATGCTTGCAGAAGAAATGTTTTGGGAATGTCTCAGTTTGCATTTACA
  AAGAAATCCAAGGAGACAGAGCCGTTTGTCTCCTTTTTGCCTCGTGTGGTCCCGGAACCCAACCTTGTGT
  TGAACTTCAGTGCCACCGCCCTCAGGAACAGTGCGGTGAAGTACTTTGTGGTGAGGAACCCGACTCCCCA
  GCCTGTCTCCCTGCAGTTGCTGCCTCTCTCCCTATACCCGAGGCCTGAAGCCGCAGTGCGCCTGCTCCAC
  AAATGGTTTGGCACAGATATGCAGATGGTTAATCTCTCAACTGGTGAATTCCAGCTCACGCAAGCTTGCC
  CTTACCAGGGCGAACCTTCTGAGGAGTCCAGTTTGGGAGCTCTTCATGTGCACTTGCAGGCTTTGGAAAC
  GAGAAGGGTTGGTGTTGTGTTCACACCAGCAGACTATGGCAAAGTCACCTCCCTCATCCTGATCAGGAAT
  AACTTGACTGTCGTTGACATGGTTGGTGTGGAAGGATTTGGAGCACAAGAGCTCTTGAAAGTGGGAGGAA
  GACTTCCTGGTGCAGGAGGCTCTCTCCGATTTAAGGTGCCTGAGTCCACCCTGATGGATTGTCATCGACA
  ACTGAAAGATAGCAAGCAGATTTTATCTATTACAAAGAACTTCAAAGTTGAGAATATCGGACCTCTTCCC
  ATAACTGTGACATCCTTGAAAATCAATGGGTATAACTGCCAGGGTTATGGATTTGAAGTGCTAGATTGCC
  ATCCGTTTTCACTGAGCCCAAACACATCCCGAGACAGCAGCATCGTGTTTACTCCAGACTTTACCTCTTC
  CTGGGTCATCCGTGAGCTGACGCTGGTCACTGCAGCAGACCTAGAATTCCACTTCACACTCAATGTGACC
  CTCCCTCACCACATGCTGCCCTTGTGTGCGGAGGTGGTTCCAGGTCCCAGCTGGGAGGAGTCCTTTTGGA
  GGCTCACTGTCTTCTTTGTCAGTTTGTCCCTGCTGGGTGTGATTCTAATAGCCTTCCAGCAAGCACAGTA
  CATTTTGATGGAATTCATGAAAACAAGACAGAGGCAAAACGGCAGCTCCTCTTCCCAGCAGAACGGTGAT
  CCAGTGGCTATGATCAGTTCCCATCCTCACAACACTTGCAAGAACTTCCTGGACACATACAGCCCATCTG
  ACAAAGGTAGAGGGAAGAGCTGCCTTCCAGTGGGCCCATCCCTGAGCAGGCTGCAGAATGCCGCCAAGAG
  GAGCCCGGCCACCTATGGTCATTCTCAGAAGAAGCATAAATGCTCCTTCTACTACAGCAAACAGAAGCCC
  AGTGCATCTGCGGCCAGCAGTGCCAACGTTACCACTGAGGAGAAGCAGACTGTGACCCTGGCAAGCTCCC
  TGTCTGTGGCCAAGGAAGACATTTGCACTAATGTCCTCAGTGAGAACTGGGTCAGCCTCAGATATGCCAG
  TGGCATCAATGGCAGCTTGCAGAAGAATCTAACCCTTCCCAAAAATGTACTGCACAAAGAGGAAAGCTCA
  CTGAAAAACACAGTTGTCACTAACACTCCTTCAGAATGTAGTATGAAGGAGGGAGTACACACATATATGT
  TTCCTAAGGAAACTGACAGCAAAATTTCAGAGAATGTGGCTGAGCTCAAGGAACAAGAGCCCTGTCCACA
  GAAGACTTCTAAGAAGCCACCTGAAAGTACTTTGCCCAAAACCCCACCTCAGTACCTTCAGTCAGACTTG
  CCAGAAGTTTCCAGGAAACATGGAAATAAGCAGCAAGCGCCTGTCAGGAGTGAAGTGGACAGCTTTGAGC
  CTGTGAGAGCCGCTGACGCAGAGCCGTCCTCAGTGAGGAAGACGCAGGGGGCATCTCCAGAAGACACGTG
  CTCCGAGAAGCAGGACACACCTTCAGCAGAGCAGGAAGATCCTTCCAGGAAGAGGAAGCTTCAGGAGAGG
  AGAGAAGGAAGCACACAAGCCCTCAACTGGAATAAAACCCGACCTTGTAGGAGAAACAAGAAAAGGGCCT
  CTGCCCAGGCCTCCAGTTCTCCCAGGCCTTCTGAGCAGAGCGAACAGAGGCTTGTCTGCAGTGACGTCAG
  AAGCTGGTGTGCACAGGACGGCGCTGGGGAGAAATGTAAGGCTGGCACGGAAGTTTCGGGCAGCTCCCCT
  GAACGGAGAGAGGAGGGAGATGGTGTTTCCCCACAGAATTTCCCTTCAGAAGCTTCGGTCCCCTTGAGCC
  TCCCACAGCACGTCTGCAGCTCTACAGATGTGAGTGTCCTGCCTGAGTTCACAGAGTCCCCCTGTCCTGG
  TCTTCCTGCCACACCTGCAGGTGCTGGAGAAGAGAAAGGTCTGTATCCACCCGGAGGCCTGTGGCCTTCC
  CAGCCAGTGTGTCTGACCAGCAGCTTCAACTGCCCAGTGGAGAACGGTGCCCCAGGGGTGTCTCAGGAGC
  CCACCTCCATCCCTGACAGTTTCATTGATTGGAGTGCATCCTGCGAAGGCCAATTTCCCAGTGTGTACTG
  CCCTCTGGAACTGAACGATTACAATGCCTTCCCAGAAGAAAACATGAATTATACCAATGGCTTCCCCTGC
  TCCTCGAAAGTTCAGACGGACTTCATTGGCCACAGCACCCCCTCTACCTGGAACACTCCGGCCAGCATGC
  CCGCTGCCTGGGGCCATGCCAGTCTCGTCAACTCTCCATCCTACCTCACGAGCACCCGAAGCTTGTCTCC
  CATGTCTGGACTCTTTGGTTCCATCTGGGCCCCACAAAGTGAAGTGTATGAAACCTGCTGCCCCATCAGC
  CCTGCCACAGAGCATGCAACCCACATGGAAAACCAGGTCATGTGCAAGGAGTACTACCTGGGCTTTAACC
  CGTTCCGTGCCTACATGAACCTGGACATATGGACTAGCACAGCAAATCGCAATGCAAACTTCCCCCTGTC
  CAGAGACTCCAGCTACTGTGGGAACATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC062940
• Insert Size: 4161 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062940, AAH62940
• RefSeq Size: 4792 bp
• RefSeq ORF: 4160 bp
• Locus ID: 229473
• Cytogenetics: 3 E3-F1
• Gene Summary: In its membrane-associated form, antagonizes canonical Wnt signaling by triggering lysosome-dependent degradation of Wnt-activated LRP6. Regulates thymocyte proliferation.[UniProtKB/Swiss-Prot Function]