Col2a1 (BC052326) Mouse Untagged Clone

CAT#: MC224565

Col2a1 (untagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col2a1
• Synonyms: Col2; Col2a; Col2a-1; Del1; Dmm; Lpk; Rgsc856
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCATCGCCGCGGTCCTACGGTGTC
  AGGGCCAGGATGCCCGAAAATTAGGGCCAAAGGGGCAGAAAGGAGAACCTGGAGATATCAGAGATATCAT
  AGGACCCAGAGGACCTCCTGGCCCTCAGGGACCTGCAGGTGAACAAGGACCCAGAGGTGATCGTGGTGAC
  AAGGGAGAAAAGGGTGCGCCTGGACCCCGTGGCAGAGATGGAGAACCTGGTACCCCTGGAAATCCTGGCC
  CCGCTGGCCCTCCAGGTCCCCCTGGTCCCCCTGGCCTTAGTGCAGGAAACTTCGCGGCTCAGATGGCTGG
  AGGGTATGACGAGAAGGCTGGTGGTGCCCAGATGGGAGTCATGCAAGGGCCCATGGGCCCCATGGGACCC
  CGTGGACCCCCAGGCCCTGCCGGTGCCCCCGGCCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGCG
  AGCCTGGTGTCTCTGGTCCCATGGGTCCCCGAGGTCCTCCTGGCCCTGCTGGAAAACCTGGTGACGACGG
  TGAAGCTGGGAAGCCCGGAAAGTCTGGGGAAAGAGGCCTCCCTGGCCCTCAGGGTGCTCGTGGATTCCCA
  GGAACCCCGGGTCTCCCCGGTGTCAAGGGTCACAGAGGTTACCCAGGCCTCGACGGTGCTAAGGGGGAAG
  CTGGTGCTCCGGGTGTGAAGGGTGAGAGTGGTTCCCCTGGTGAGAACGGATCCCCGGGCCCAATGGGTCC
  CCGTGGCCTGCCTGGTGAGAGAGGACGGACTGGCCCTGCTGGTGCTGCTGGTGCTCGGGGTAACGATGGC
  CAGCCAGGCCCCGCTGGACCTCCGGGTCCTGTGGGTCCCGCAGGTGGTCCTGGCTTCCCTGGTGCTCCTG
  GTGCCAAGGGCGAAGCTGGTCCCACTGGTGCTCGCGGTCCTGAAGGTGCTCAAGGTTCTCGTGGCGAGCC
  TGGCAATCCTGGGTCCCCTGGGCCTGCAGGTGCTTCTGGTAACCCAGGGACTGATGGTATTCCTGGAGCC
  AAAGGATCCGCTGGTGCTCCTGGAATTGCTGGTGCCCCTGGCTTCCCTGGGCCCCGTGGCCCTCCCGGTC
  CTCAAGGTGCAACTGGTCCCCTTGGCCCCAAAGGTCAGGCGGGTGAACCTGGCATTGCTGGCTTTAAAGG
  TGATCAAGGCCCCAAGGGAGAGACTGGACCTGCTGGGCCCCAAGGAGCCCCTGGCCCCGCTGGTGAAGAA
  GGCAAACGAGGTGCTCGAGGAGAGCCGGGTGGTGCTGGACCAATCGGACCCCCTGGAGAGAGAGGTGCTC
  CTGGCAACCGTGGATTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGTGCCCCTGGAGAGCGAGGGCC
  CAGTGGCTTGACTGGTCCCAAGGGAGCCAACGGTGACCCGGGTCGTCCTGGAGAACCTGGTCTTCCTGGA
  GCCAGGGGTCTTACCGGTCGCCCTGGTGACGCTGGTCCTCAAGGCAAAGTTGGTCCTTCTGGAGCCCCTG
  GTGAAGACGGTCGCCCTGGACCTCCTGGTCCTCAGGGAGCTCGTGGGCAGCCTGGCGTCATGGGTTTCCC
  TGGCCCCAAAGGTGCCAACGGCGAGCCTGGCAAAGCTGGTGAGAAGGGTCTGGCTGGCGCTCCTGGTCTG
  AGAGGTCTTCCTGGAAAAGACGGTGAGACGGGAGCCGCAGGACCCCCCGGCCCCAGTGGACCTGCTGGTG
  AACGAGGCGAGCAGGGCGCTCCTGGACCATCAGGGTTCCAGGGACTTCCTGGCCCTCCCGGTCCCCCAGG
  TGAAGGTGGAAAGCAAGGTGACCAGGGTATTCCTGGTGAAGCTGGAGCTCCTGGCCTTGTGGGTCCTCGG
  GGCGAGCGAGGTTTCCCAGGTGAACGTGGCTCTCCTGGTGCTCAGGGCCTTCAGGGTCCCCGAGGCCTCC
  CTGGCACTCCTGGTACTGATGGTCCCAAAGGTGCAGCTGGCCCAGATGGCCCCCCTGGGGCTCAGGGGCC
  TCCAGGTCTACAGGGAATGCCTGGTGAGAGAGGAGCCGCTGGCATTGCTGGGCCCAAGGGAGACAGAGGC
  GATGTTGGCGAGAAAGGCCCAGAGGGAGCTCCTGGGAAGGATGGCGGCCGAGGTCTGACTGGGCCCATCG
  GACCCCCAGGCCCAGCAGGGGCCAACGGCGAGAAGGGAGAAGTCGGACCTCCTGGCCCGTCAGGAAGTAC
  CGGAGCTCGAGGTGCCCCGGGTGAACGCGGAGAGACCGGGCCACCTGGACCTGCTGGATTCGCTGGCCCT
  CCTGGTGCTGATGGCCAGCCTGGTGCCAAGGGTGATCAAGGAGAAGCCGGACAGAAAGGAGATGCTGGTG
  CCCCCGGCCCACAAGGCCCCTCGGGAGCCCCTGGGCCACAGGGTCCTACTGGAGTGACTGGTCCTAAGGG
  AGCCCGAGGTGCCCAAGGTCCCCCGGGAGCCACCGGATTCCCTGGAGCTGCTGGCCGAGTTGGACCCCCA
  GGTGCTAATGGCAATCCTGGACCCGCCGGTCCCCCTGGTCCTGCTGGAAAAGATGGTCCCAAAGGTGTTC
  GAGGAGACAGTGGCCCCCCTGGCAGAGCTGGTGACCCCGGTCTTCAAGGTCCTGCAGGAGCTCCTGGCGA
  GAAAGGAGAACCTGGAGATGATGGTCCCTCTGGTCTTGATGGTCCTCCAGGTCCCCAGGGGCTGGCTGGT
  CAAAGGGGCATTGTTGGTCTGCCTGGTCAGCGTGGTGAGAGAGGATTCCCCGGCCTTCCCGGCCCATCGG
  GTGAGCCCGGCAAGCAGGGTGCACCTGGCGCGTCTGGAGACAGAGGTCCTCCTGGTCCTGTGGGGCCTCC
  TGGCCTGACAGGGCCTGCAGGTGAACCTGGACGAGAGGGCAGCCCTGGTGCTGATGGACCCCCTGGAAGA
  GATGGTGCAGCTGGAGTCAAGGGAGATCGTGGTGAGACTGGAGCACTGGGTGCCCCTGGAGCTCCTGGGC
  CCCCAGGCTCTCCTGGTCCTGCTGGCCCAACTGGCAAACAAGGAGACAGAGGAGAGGCTGGTGCACAAGG
  TCCTATGGGTCCCTCAGGACCTGCTGGAGCCCGTGGGATTGCAGGCCCTCAAGGCCCCCGAGGTGACAAA
  GGAGAATCTGGAGAGCAGGGCGAGAGGGGACTGAAGGGACACCGAGGTTTCACTGGACTGCAGGGTCTGC
  CTGGCCCTCCGGGTCCTTCTGGAGATCAGGGTGCTTCTGGCCCTGCTGGTCCTTCTGGCCCTAGAGGTCC
  ACCTGGCCCTGTTGGTCCCTCTGGCAAAGATGGCTCTAATGGAATCCCTGGCCCCATCGGGCCTCCAGGT
  CCCCGTGGACGCTCAGGAGAAACAGGCCCTGTTGGTCCCCCTGGAAGTCCCGGTCCTCCTGGCCCTCCAG
  GTCCTCCTGGTCCTGGCATCGACATGTCAGCCTTTGCTGGCTTAGGGCAGAGAGAGAAGGGCCCCGACCC
  CATGCAGTACATGCGGGCCGACGAGGCAGACAGTACCTTGAGACAGCACGACGTGGAGGTGGACGCTACA
  CTCAAGTCACTGAACAACCAGATTGAGAGCATCCGCAGCCCCGACGGCTCCCGCAAGAACCCTGCTCGCA
  CTTGCCAAGACCTGAAACTCTGCCACCCCGAGTGGAAGAGCGGAGACTACTGGATTGATCCCAACCAGGG
  CTGCACCTTGGACGCCATGAAAGTTTTCTGCAACATGGAGACCGGCGAGACTTGCGTCTACCCCAACCCA
  GCGACTGTCCCTCGGAAAAACTGGTGGAGCAGCAAGAGCAAGGAAAAGAAACACATCTGGTTTGGAGAGA
  CCATGAACGGTGGCTTCCACTTCAGCTATGGCGATGGCAACCTGGCTCCCAACACCGCTAACGTCCAGAT
  GACTTTCCTCCGTCTACTGTCCACTGAGGGCTCCCAGAACATCACCTACCACTGTAAGAACAGCATCGCC
  TACCTGGACGAAGCGGCTGGCAACCTCAAGAAGGCCTTGCTCATCCAGGGCTCCAATGATGTAGAGATGA
  GGGCCGAGGGCAACAGCAGGTTCACATACACTGCCCTGAAGGATGGCTGCACGAAACACACTGGTAAGTG
  GGGCAAGACCGTCATCGAGTACCGATCACAGAAGACCTCCCGCCTTCCCATTATTGACATCGCACCCATG
  GACATTGGAGGGGCTGAACAGGAATTTGGTGTGGACATAGGGCCTGTCTGCTTCTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052326
• Insert Size: 4260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052326, AAH52326
• RefSeq Size: 4856 bp
• RefSeq ORF: 4259 bp
• Locus ID: 12824
• Cytogenetics: 15 53.97 cM
• Gene Summary: This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015]