Med14 (NM_001048208) Mouse Untagged Clone

CAT#: MC224496

Med14 (untagged) - Mouse mediator complex subunit 14 (Med14), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med14
• Synonyms: 9930001L01Rik; AU041628; Crsp2; ENSMUSG00000073278; Gm641; ORF1; Trap170
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224496 representing NM_001048208
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCAGTGCAGCTGGACAACCACCAGCTGATCCCTCCTGGCGGCGGCGGTGGCAGCAGCGGCGGCG
  GAGGCAGCAGCAGCGGCTCCGCGTCTGCCCCGGCTCCCCCGCCGCCCGCAGCCGCTGTGGCGGCGGCAGC
  TGCGGCGGCAGCTAGCCCTGGATACCGGCTTAGCACGCTCATCGAATTTCTGCTCCACCGGGCCTACTCG
  GAGCTTATGGTGTTGACGGACTTATTGCCAAGGAAATCTGATGTGGAAAGGAAAATAGAAATTGTACAGT
  TTGCTAGCCGGACACGTCAACTCTTCGTTCGATTATTAGCTTTAGTAAAATGGGCCAATGACGCTGGCAA
  AGTAGAAAAGTGTGCGATGATCTCAAGCTTTTTAGATCAGCAAGCTATCTTATTTGTGGATACCGCTGAC
  CGCTTGGCCTCCTTAGCTAGAGATGCCCTGGTCCATGCACGCCTGCCTAGTTTTGCTATTCCATATGCCA
  TTGATGTACTGACTACTGGCTCTTATCCACGGCTTCCAACCTGCATCAGGGACAAAATTATTCCTCCAGA
  CCCAATTACCAAAATTGAGAAACAAGCCACACTTCATCAGCTTAATCAGATTCTTAGACATAGGCTTGTA
  ACGACAGATCTTCCTCCACAGCTAGCAAATCTTACAGTGGCAAATGGCCGTGTGAAGTTTCGAGTTGAAG
  GAGAATTTGAAGCAACCTTGACAGTGATGGGTGATGACCCAGAAGTCCCGTGGCGTCTTCTCAAGCTAGA
  AATTCTAGTTGAGGATAAGGAAACAGGAGATGGGCGAGCTTTGGTTCATAGCATGCAAATCGATTTTATC
  CATCAGCTGGTCCAGTCTAGGCTCTTTGCTGATGAGAAACCTCTTCAGGACATGTACAACTGCCTACATT
  GTTTCTGCTTATCGCTTCAACTAGAAGTATTACATTCCCAGACACTAATGTTAATCCGAGAGAGGTGGGG
  AGACCTTGTACAGGTGGAAAGATACCATGCTGGAAAGAGCCTCTCCCTCTCAGTTTGGAATCAACAGGTT
  CTAGGGAGAAAAACAGGCACAGCATCTGTTCACAAAGTTACAATTAAAATCGATGAGAATGATGTCTCCA
  AGCCTTTACAGATTTTTCATGATCCTCCTTTGCCAGCTTCTGATTCTAAATTAGTAGAAAGAGCCATGAA
  GATTGACCACTTATCAATAGAAAAGCTCCTGATTGACAGTGTACACGCAAGAGCCCACCAGAGGCTGCAG
  GAACTGAAGGCCATTCTTAGAAGCTTCAACGCCAATGAAAGCTCTTCCATAGAGACTGCACTTCCAGCTC
  TTATTGTACCCATCTTGGAGCCTTGTGGTAATTCGGAGTGCCTACACATTTTTGTAGATTTGCATTCTGG
  AATGTTCCAATTGATGCTTTATGGACTTGACCCAGCCACTCTGGAGGACATGGAAAAATCTTTGAATGAT
  GACATGAAGCGGATCATCCCTTGGATTCAGCAACTTAAGTTTTGGCTGGGACAACAGCGTTGCAAACAAT
  CTATAAAACATCTGCCTACAATAACCACTGAAACGTTGCAGCTTGCCAACTATTCAACACATCCCATTGG
  AAGCCTTTCTAAGAATAAGCTGTTTATTAAACTTACTCGCCTTCCACAGTACTACATTGTTGTGGAAATG
  CTTGAGGTTCCTAATAAGCCTACGCAGCTATCATATAACTATTACTTTATGTCGGTGAGTACTGCAGATC
  GTGAGGACAGCCCTGTCATGGCACTGCTGCTGCAGCAATTCAAGGACAACATCCAGGACCTGATGTCCTA
  TACAAAGACTGGGAAACAGACCAGAACTGGTACCAAGCACAAGTTGTCTGATGACCCATGTCCGATAGAC
  TCCAAGAAAGCCAAACGATCAGGAGAAATGTGTGCCTTCAATAAAGTTCTAGCTCACTTTGTTGCTATGT
  GTGACACCAACATGCCATTTGTTGGTCTTCGATTGGAGTTGTCTAACCTCGAGATACCACATCAGGGAGT
  GCAAGTGGAAGGTGATGGCTTCAACCATGCAATTCGCTTATTAAAGATTCCTCCCTGTAAAGGAATAAGT
  GAGGAAACACAGAAGGCTCTGGACCGCTCTCTTCTTGATTGCACTTTCCGATTACAAGGTAGAAATAACC
  GCACTTGGGTGGCTGAATTAGTGTTTGCAAATTGTCCGCTTAATGGCACTTCCACCAGGGAGCAAGGACC
  ATCCCGACATGTTTACCTGACGTATGAAAATTTGTTGTCTGAACCTGTTGGTGGCAGAAAAGTAGTTGAA
  ATGTTTCTTAATGACTGGAGTAGCATTGCCCGTTTATACGAGTGTGTGTTGGAATTTGCACGTTCTCTAC
  CAGAAATACCTGCTCATCTGAATATTTTCTCAGAAGTTCGTGTTTATAATTATCGAAAGCTTATCTTATG
  TTATGGAACCACCAAAGGAAGCTCAATTAGTATTCAGTGGAACTCCATTCATCAGAAATTTCACATTGCG
  TTGGGAACAGTTGGCCCAAACTCAGGTTGCAGTAACTGTCACAATACCATTCTCCATCAACTTCAAGAAA
  TGTTCAACAAAACACCAAATGTGGTTCAGTTATTACAGGTTTTGTTTGATACTCAGGCACCATTAAATGC
  TATCAACAAACTCCCTACTGTTCCAATGTTGGGCTTGACCCAGAGAACTAACACCGCCTACCAGTGTTTC
  TCCATTTTACCACAATCATCCACCCACATCAGACTGGCCTTCAGGAACATGTACTGCATTGACATATACT
  GCCGTAGTCGAGGCGTCGTGGCAATACGGGACGGTGCCTATAGTCTTTTTGATAACAGCAAGTTAGTTGA
  AGGCTTCTATCCTGCACCAGGATTAAAGACTTTCCTGAACATGTTTGTTGATAGCAATCAAGATGCTCGG
  AGAAGATCTGTAAATGAGGATGATAACCCTCCTTCTCCTATAGGAGGTGACATGATGGACTCTTTAATAT
  CACAGCTCCAGCCACCTCAGCAACAGCCATTTCCAAAGCAGCCAGGGACATCAGGCGCTTACCCTCTTAC
  TTCACCCCCTACATCCTATCACAGCACAGTTAATCAGTCCCCCTCTATGATGCACACACAGTCTCCAGGA
  AATCTGCATGCTGCCAGCTCCCCCAGTGGGGCTTTGAGAGCCCCATCACCAGCGTCATTTGTTCCAACTC
  CTCCCCCATCCTCGCATGGAATCTCAATAGGACCAGGGGCCAGTTTTGCTAGTCCACATGGAACCCTTGA
  CCCTAGTTCTCCATATACTATGGTGTCACCAAGTGGACGAGCAGGAAACTGGCCTGGGTCACCTCAAGTG
  TCCGGACCCTCACCAGCAACCCGTTTGCCTGGAATGTCACCAGCTAACCCATCTCTGCATTCTCCTGTTC
  CAGATGTTTCTCATTCTCCTCGAGCTGGCACAAGTTCCCAGACAATGCCAACCAACATGCCTCCACCTCG
  AAAACTACCTCAGCGCTCCTGGGCAGCATCTATACCCACCATTCTCACTCATAGTGCCTTGAACATCTTA
  CTGCTACCATCTCCAACGCCAGGCCTTGTGCCTGGCCTGGCAGGTAGTTACCTTTGTTCCCCACTTGAGA
  GATTCCTTGGATCTGTCATCATGAGACGACACCTTCAAAGAATCATCCAACAGGAAACGTTGCAGCTGAT
  CAATTCCAATGAACCTGGTGTAATCATGTTCAAGACAGATGCCCTGAAATGCAGAGTAGCCCTTAGTCCC
  AAAACCAACCAGACCCTTCAGCTAAAAGTGACACCTGAAAATGCAGGACAATGGAAACCCGATGAGCTTC
  AAGTTTTGGAGAAATTCTTCGAAACAAGAGTTGCAGGACCGCCATTTAAAGCCAACACACTTATAGCCTT
  CACCAAGCTGTTGGGAGCGCCGACACACATCCTCCGGGACTGTGTGCATATAATGAAGTTGGAGTTGTTC
  CCAGATCAAGCAACGCAGCTGAAATGGAACGTTCAGTTTTGCCTCACCATCCCTCCCAGCGCACCACCAA
  TTGCACCGCCTGGGACACCAGCTGTGGTCCTGAAATCCAAAATGCTATTTTTTCTTCAACTAACTCAGAA
  AACATCAGTCCCTCCCCAAGAACCTGTTAGTATAATAGTTCCAATTATTTATGATATGGCTTCAGGTACC
  ACTCAGCAGGCAGACATTCCCAGACAGCAGAACTCTTCTGTTGCTGCGCCAATGATGGTCAGCAACATTC
  TGAAGAGGTTTGCAGAGATGAACCCTCCACGACAAGGTGAATGCACAATATTTGCAGCTGTTCGTGATTT
  AATGGCTAATCTTACACTGCCCCCTGGTGGGCGTCCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001048208
• Insert Size: 4380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048208.1, NP_001041673.1
• RefSeq Size: 6861 bp
• RefSeq ORF: 4380 bp
• Locus ID: 26896
• UniProt ID: A2ABV5
• Cytogenetics: X A1.1
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]