3425401B19Rik (NM_001195097) Mouse Untagged Clone

CAT#: MC224428

3425401B19Rik (untagged) - Mouse RIKEN cDNA 3425401B19 gene (3425401B19Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 3425401B19Rik
• Synonyms: Cefip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224428 representing NM_001195097
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGGGAAACAAAAAATGCACAGACGGGTTCAGTGATACCTCCAGCATCGGCAGTGTGCTGGATG
  AAGCAGACAGGGAGGTGAGCAACCTCACAGACAGGGCATTCCGAAGTCTGTGCATCTCGGAGGACACATC
  CTTCCATGACTCTGACCTGGCCCTGTCCCCAGATGTCACCAGCCAGGTGTCGGGGACTTTTCACCAGGAA
  ACAGTGGGCCATGCCAACAGGAAAAGTGGGATTTGGAGCCAGCTTCCATCTCAAGGCACAGAGCATTCTG
  GCTGGGCAGCCACCTTCCAACAGCAACCCAAGTACGTTCAGGGCGAAGAAAAGTACCCCAAAACCAGTCC
  TCTGCCAACCCCAGTCCAGAGGAGACTGGAGGTGCCCATTTCAGGCTTGAGGAGCAGCAGCAAACCCATC
  TCCAAAGTCTCATCACTCATTAGATCCTTTGACAGGACAGAGACTCAATCTTGTGACAGCCGGCCTCCTC
  CCAGCAAGCCTCCGGCTCTCAAAAATCCCCCCAAGTTTGCACATCCCCCAGAAAGTGGTGTCAACTTCTG
  CTTCGATTCGGCCTTTCTGACCGTCAGGAGGGTGCCTGCTGAAGTCTCCAACACCCATCAGGGCAGCCAT
  CAGTCTGGCAGGGCTCCTGGAGAACAAGAGTCTCCCAAGAACCCAGAAATAGCCTCTCACAGCTCAGACA
  GCCTCCTCCGGACACCTGACCATGTGGCTGGCTCATTTGAGCCAAGGTATCCCTCTCCACCTCTCAAGCC
  AGCCACAGCTGAGCCAGGAAGAGGGAAGGAGTGGATTCCAAGGAGGACTTTTCTGCACAGTGAAAACAGT
  GCCTTTGAGTCGTGGGACACCCACCAACCAAAGCTCCGGGAGAGAAAGGACATTGCTGAAACCACCCCAG
  AAAGCAAAGCCCCCAAACATTATGAAGACATGCCTTTGCTAAAGGAGCCCTACCCTGCAGAGTCCAAAGG
  CTCCCCCTACCAGGCCCGAGCTAACTGTGCCCAGGAAGAGAACAGGTCACCGTCAGGGAGCCAATCCACA
  TCTGGAGCCTGGGGTGCCAGGGATCCAGGATCCCAGCTATTCCCTGTAGAGGGGAATGCTTCACAGATTG
  ACCCTCAGGTGAAGCGGAGTAAGGCCCCATGGAGGAAGCCAAAGACTGGCAAAGGAGGGACAGATGGTCC
  ACCTGATGCTTTGGAAGACAAGAAACAGCCCAATAGGAGAGGTCTACCTCTGTATTCAAAACTTAACCCT
  CAGGGTCAACTTCCAGAAAACGGTGTTCTAGACATGCCAGAAGAGTCCAATGACCATTACAATCCCCCTT
  TTAACATCAGCAAACTCCTCACCCCAATTATATCTACAAAGCATGTCCTGGAAACTTCAGACACCCAACC
  AGTGGAAATAAGCCCATCCCCTCCAGGACAGCTAAATGGATACCAGGAGAAGGAGTCCAGTGAGGCTCAG
  TCTCGGGATAGCTACAAATCCAAAGCGCCCAGCCTGCTGTTCAACCTAAAAGATGTTCGGAAACGTGTCA
  AGAGTACATACAGCCCCTTGCCTCTCTTAAAAGGCTTTGATGAGAAAACCAGAGGTAAACTTGATAGCAA
  GCAAGAACCTCTGAGCAATGGGGTCACCCTTCCCGATGGGCTTGAAGAAAACCCTCCCACAGAGCTCTTG
  CCAGATAATGTCCCTGGTGTTTTGCACAGCAGCACCCAGAAGGATCCTGCTATCAACTCTAGAGAATCCT
  TTGCAGTCAGCCACCCGACCTTCAGTTCACCTTCAGCTAGCTCCCAAACCCACTTCTGTGTCAATGGGGA
  AGCTGCAGAAAGTAACAGTAATGAGAAGGAAGAAGCCAACGGAGAGTCAGAGCTGGATCCCTCCAAGGGT
  GGCGGGCATCCAGACTGCAGGGAAAACCTTCCTCGGAAACATCTCTCCCTGAAACTCTTCAACAGGGAGT
  CTGAAATGGGACCGGCTATGGCGGAAATGAAGCCCCACCAGCTAGAGAATGGGCTCTCGAGATCTGTCTC
  CCAGGAGACAGAGACTGAGCGAGAAACGGGGTTCCAGAGTCTACCCTTGAACCAGAAGTTCTCCCCAGGA
  CCCCTTTCCCCTGAGGAGGAGGATGTGTTTTACAGCGACAGCCAGTCTGATTTTACTCCGTGCCGCCAAA
  CAAAGGCTAAATTCAGCACCAGCTCCTCAGACCAATCCTTTGCTTCTTTTGAAGATCAGCAGAAGGTATG
  CTTTACAGAAGGCCCCCAGGAAGACAGGAAGAGCCATGTGAGTGCAGGTGACAAGCAGAGGGACGAGACG
  GCGGTGGAGAAAGAAGAGTCACAGCAATGTGCCTCACGTAATGAACACAGAGGTGTGGACGAGCAGAGAC
  AGGAGGAAATACAAAGAAAAGCACAAGGTGTTTCAGGAGGAAGACCCAGGAAAGCATCCGCAGAGGACCT
  CAGTGCCAGAGGCTCGTGGATGGGGGCTGATAAGGACACAGCTCATACACACGCCAAGGACCCAACCCCC
  TTGCCAGCCTCTACCAACAAGCACAGACTGTTCCCAATTAAAGACAACACACTCAGGGCCACCCCGGTGA
  TAAAGCCTATTATTCTGCCTCTCCTGAGGACAGTCTCCTCAGAGGACTCACTGAGCAGTGGACACCAAGA
  GAATGAATTGCCAAAGCAACTGTGGGGCGAAGATGCTGGCGGCCTTAGTGCTTCAGAAAGCCAAGAAATG
  CCTAACACCCCACTCTCCAATAACGACGTGCCGGGCACCCAGCACAGGTGTATGGTGTGTGAGGACGTGC
  AGGAAGACCCGGTACACACCGCAGCCCAGGATGAGACTTCTCAGCAAACCCGGAAGGGGAGTTTCTCTTT
  TCTACCACCGGTGGAAGAGGAAAACAGAATGAAACCATCCCCAGATACGGCAGGTGAAGGGCTAGCACAA
  GAGAAAAGCAAATCAGCAGACTTGGGGAAACTGGGGGTTCCACAGCGTATCCCCACTATCGCTTTACTCC
  CGGATGACTTAGAAGACTCGCCCCCATCGCTACCACAACATACCTATTGGGAAGAACAAGGTTTCAAAGG
  CCACTTTTTGTCTGCACCGAGAGCAGGACCGTCTGGAAGAAGGCTTGTCCCCAGTGAGGCAGAGACTTCC
  CCCAACCCCAGCTCTCTAGGAGAGAGCAGCACTTGCTCTCCCGCAGCCAGCAGTATTTGGGAAGAGGCTT
  CCCAGGCCGCTGGGGAACACTGGCAGCGCCAAGAGCCTCCTGGCCCCAACCCCTGGGCCAGCCCGGGCCC
  TACGGGGCTAACTCGGAGGGAGGACATGACCCATGGCCTCACATGGGAAGCTGAAGGTTCGGATCCCTCA
  GATTTCAGGGCCCTCTCTCCGAGAGGCATTTTGCTAGCTGATGCTGCTGAGAAACCTGAGCCCCCTGCTC
  TGCTGGAGAAGGCGGCAGGCAAGCCCCCTGCAGTCCCTCCCAAGACGGAGAAGGCCCTGCGCAGGGCGAA
  GAAGCTGGCAAGCAAGAGGAGGAAGAGTGATCAGTTGCTGGAGAAGCATACGGAGGCCTGGGAGGGCAAG
  TCCTTCACAGAGGACACTCAGGGGACAGAGCGAAGGCCTGTGTCTCCTGGAAAGGGGCCTCGGCCCAGGT
  TCCCTGCCATTCGCTCCTTGCCCCCGCCCACGCACCGCCACTCAGTGTCCTGTGGCTGGGAGCCCACAGG
  GAGGCGGCCTTGGGGACCCCAGTCCCTCACGCCTCTTCCCCCTTACCCCGCCACCCAGAAGGTGCTCCAG
  GATCCCCAGTCAGGACAATACTTCGTCTTCGACGTGCCTCTCCAAGTGAAAATCAAGACTTTCTATGACC
  CTGAGACTGGCAAGTATGTCAAGGTCTCTGTCCCATCCTCTGAGGAGGCCTCCTCAGAGCCACCTCTGCA
  GGATGCCCTGGCTGCTCCCTACCTACTGTACCCCGGCTTCCGGCCAGTGCCTGTGACTTCCGTGATGCCC
  CTGCGCTGCTCCTCTCAGCTTGCAGCCCCCACCTTTCTCAGGCAGGGTTCTGGCCATAGGCCCCAGAGTT
  CCCAGGGCAGTCGACTGCAGCCACCCCCTGAACGGCTGGGCGAGTCTACCCAGCATGCCTCTGGCCAGTG
  CCCCCGTGGGCCTTCCCATAGTCCAGAGAAAGAGAGTGCAGAAGCTCCAAGACTAAGTATCATCTCCACA
  GACGACCTAGAGGACTTTGCCACAGAGGGTGTTTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195097
• Insert Size: 4239 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195097.1, NP_001182026.1
• RefSeq Size: 5553 bp
• RefSeq ORF: 4239 bp
• Locus ID: 100504518
• UniProt ID: D3Z1D3
• Cytogenetics: 14 B
• Gene Summary: Plays an important role in cardiomyocyte hypertrophy via activation of the calcineurin/NFAT signaling pathway.[UniProtKB/Swiss-Prot Function]