Pnpla6 (NM_015801) Mouse Untagged Clone

CAT#: MC224237

Pnpla6 (untagged) - Mouse patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pnpla6
• Synonyms: AI661849; MSws; Nte
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224237 representing NM_015801
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCCCACTGCAAACCGGAATGGTGCTGGGCGTGATGATCGGGGCCGGAGTGGCGGTGCTGGTCA
  CCGCCGTGCTCATCCTCTTGGTGGTGCGAAGGCTGCGGGTGCAGAAAACTCCAGCCCCGGAGGGTCCCCG
  GTATCGGTTCCGGAAGCGGGACAAAGTGCTTTTCTATGGCCGGAAGATTATGCGGAAGGTGTCACAGTCC
  ACTTCTTCCCTGGTGGACACATCAGTCTCCACCACTTCCCGGCCCCGCATGAAGAAGAAACTGAAGATGC
  TTAACATTGCCAAGAAGATCTTACGTATCCAAAAGGAGACTCCGACCCTACAACGGAAGGAGCCCCCACC
  TTCAGTGCTGGAGGCTGACCTCACAGAGGGAGACCTGGCCAATTCCCACCTACCCTCTGAGGTACTCTAC
  ATGCTTAAGAATGTTCGGGTGCTAGGTCACTTTGAGAAGCCGCTCTTCCTGGAGCTCTGCCGGCACATGG
  TCTTCCAGCGTCTCGGGCAGGGGGACTATGTTTTTCGGCCCGGTCAACCAGATGCCAGCATCTATGTGGT
  TCAAGATGGGCTGCTGGAGCTCTGCCTACCAGGGCCTGATGGGAAGGAGTGTGTGGTGAAGGAGGTGGTT
  CCTGGAGACAGCGTCAACAGCCTTCTGAGCATCCTTGATGTCATCACGGGTCACCAGCATCCCCAGCGGA
  CTGTGTCAGCCAGGGCTGCACGGGACTCCACAGTGCTGAGACTCCCAGTAGAAGCCTTCTCTGCTGTCTT
  CACCAAGTACCCTGAGAGTTTGGTGCGAGTGGTACAGATCATCATGGTAAGGCTGCAGAGAGTCACCTTC
  CTGGCGCTTCACAATTACCTGGGTCTGACCAATGAACTCTTCAGTCATGAGATCCAGCCCCTACGCCTTT
  TCCCCAGCCCCGGCCTCCCGACCCGAACCAGCCCTGTCCGTGGCTCTAAACGGGTAGTCAGCACCTCAGG
  TACTGAGGACACCTCAAAGGAGACCTCTGGCCGGCCCCTGGACTCCATTGGAGCTCCTCTGCCTGGACCT
  GCAGGGGATCCCGTGAAGCCCACATCCTTAGAAGCACCCCCAGCCCCGTTACTGAGCCGCTGCATCTCCA
  TGCCAGTAGACATCTCAGGCTTGCAAGGTGGCCCTCGTTCTGATTTTGACATGGCCTATGAACGTGGACG
  GATATCTGTTTCTCTTCAAGAAGAGGCATCTGGGGGACCTCAGACAGCATCCCCTAGGACCCCCACTCAG
  GAGCTCCGGGAGCAGCCAGCGGGTGCCTGTGAATATAGCTACTGTGAGGATGAGTCAGCCACAGGCGGAT
  GTCCCTTTGGGCCCTACCAGGGCCGCCAGACAAGCAGCATCTTTGAGGCTGCAAAGAGAGAGCTAGCCAA
  GCTGATGCGGATTGAGGACCCCTCTCTACTGAACAGCAGAGTCTTGCTACATCATGCCAAAGCCGGCACC
  ATCATAGCCCGCCAAGGGGACCAGGATGTGAGCCTGCACTTTGTGCTCTGGGGCTGCCTGCACGTGTACC
  AGCGCATGATTGACAAGGCTGAGGAAGTGTGCCTATTTGTGGCACAGCCAGGGGAGCTGGTGGGGCAACT
  GGCGGTGCTCACTGGGGAACCTCTCATCTTCACACTACGTGCCCAGAGAGACTGCACCTTCCTGCGGATC
  TCCAAATCCCACTTCTATGAGATCATGCGTGCACAACCCAGTGTGGTACTGAGTGCAGCTCACACGGTGG
  CTGCTAGAATGTCCCCCTTCGTGCGCCAGATGGACTTTGCCATTGACTGGACAGCTGTGGAGGCTGGTCG
  TGCGCTCTACAGGCAGGGAGACCGCTCCGACTGCACCTACATTGTACTCAATGGACGTCTCCGTAGTGTC
  ATCCAACGAGGCAGTGGCAAGAAGGAGCTAGTTGGGGAGTATGGTCGAGGGGATCTCATTGGCGTGGTGG
  AGGCACTGACCCGGCAGCCACGAGCCACCACTGTACACGCAGTGCGAGACACTGAGCTGGCCAAGCTTCC
  CGAGGGCACCTTAGGCCACATCAAACGTCGGTACCCACAGGTTGTGACCCGCCTTATTCATCTGCTAAGC
  CAGAAAATTCTAGGCAATCTGCAGCAGTTGCAAGGACCCTTTCCAGGCTCCGGGCTCAGTGTTCCGCAGC
  ACTCGGAACTGACCAACCCAGCCAGCAATCTGTCTACTGTAGCTATCCTCCCTGTGTGTGCTGAGGTGCC
  CATGATGGCCTTTACTCTGGAACTGCAGCATGCTCTGCAAGCTATTGGTCCCACGCTCCTCCTTAACAGT
  GACGTCATCCGGGCACTCCTGGGGGCTTCAGCACTGGATAGCATTCAAGAATTCCGGCTGTCAGGGTGGT
  TGGCCCAGCAGGAAGATGCCCATCGCATTGTGCTCTACCAAACTGACACATCCCTGACGCCTTGGACCGT
  CCGTTGTCTGCGCCAGGCCGACTGCATCCTCATTGTGGGCCTGGGGGACCAGGAACCCACTGTTGGCCAG
  CTGGAGCAAATGTTAGAGAACACTGCTGTCCGTGCCTTGAAGCAACTGGTTTTGCTGCACCGGGAGGAAG
  GCCCTGGTCCCACGCGCACTGTAGAGTGGCTCAACATGCGCAGCTGGTGCTCAGGGCACCTGCACCTGCG
  CTGTCCTCGCCGCCTCTTCTCACGGCGCAGCCCAGCGAAACTTCATGAGCTGTATGAGAAGGTTTTCTCC
  AGGCGTGCAGACCGTCACAGCGACTTCTCCCGCTTGGCACGAGTACTCACAGGAAACACTATTGCTCTGG
  TGCTGGGTGGGGGCGGAGCCAGAGGCTGCTCGCATATTGGGGTGCTGAAGGCATTAGAGGAGGCAGGAGT
  CCCAGTCGACCTTGTGGGAGGCACATCCATAGGTTCCTTCATTGGGGCCTTGTATGCCGAGGAACGCAGC
  GCCAGCCGAACTAAACAACGAGCCCGGGAGTGGGCCAAGAGCATGACTTCTGTACTGGAGCCTGTATTGG
  ACCTCACATATCCTGTCACCTCCATGTTTACTGGCTCAGCCTTTAACAGAAGTATCCACCGTGTCTTCCA
  GGATAAGCAGATTGAGGACCTGTGGCTGCCTTACTTCAATGTGACCACAGACATCACTGCCTCTGCCATG
  CGCGTCCACAAAGATGGCTCCCTGTGGCGGTATGTACGTGCCAGCATGACGCTCTCGGGCTACCTACCCC
  CGCTGTGCGACCCAAAGGATGGGCACCTGCTCATGGATGGTGGCTACATCAACAACCTGCCAGCGGATAT
  TGCCCGAAGCATGGGAGCCAAAACGGTCATTGCCATCGACGTTGGAAGCCAGGATGAGACAGATCTCAGC
  ACCTATGGGGACAGCCTGTCTGGCTGGTGGTTGCTGTGGAAAAGGCTAAACCCCTGGGCAGACAAGGTAA
  AGGTTCCAGACATGGCTGAGATCCAGTCCCGCTTGGCGTACGTGTCCTGTGTGCGGCAGCTAGAAGTCGT
  CAAGTCCAGCTCCTATTGCGAGTACCTTCGTCCATCCATTGACTGCTTCAAGACCATGGACTTTGGAAAG
  TTTGACCAGATCTATGATGTGGGTTACCAGTATGGGAAGGCTGTCTTTGGAGGCTGGACCCGTGGTGAAG
  TCATTGAGAAAATGCTCACAGACCGGAGATCTACAGACCTTAATGAGAGTCGCCGTGCAGATATACTTGC
  CTTCCCAAGTTCTGGGTTCACTGACTTGGCTGAGATTGTGTCCCGGATCGAGCCACCAACAAGCTACGTC
  TCTGATGGCTGTGCTGATGGGGAGGAGTCGGATTGCTTGACAGAGTATGAGGAAGATGCAGGACCAGACT
  GCTCAAGAGATGAAGGGGGCTCCCCTGAGGGTGCCAGCCCTAGCACTGCCTCAGAGGTGGAAGAAGAGAA
  GTCCACACTCCGGCAACGACGCTTTCTGCCTCAGGAGACTCCCAGCTCAGTCGCAGATGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015801
• Insert Size: 3984 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015801.2, NP_056616.2
• RefSeq Size: 4455 bp
• RefSeq ORF: 3984 bp
• Locus ID: 50767
• UniProt ID: Q3TRM4
• Cytogenetics: 8 1.92 cM
• Gene Summary: Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy.[UniProtKB/Swiss-Prot Function]