Magel2 (NM_013779) Mouse Untagged Clone

CAT#: MC224146

Magel2 (untagged) - Mouse melanoma antigen, family L, 2 (Magel2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Magel2
• Synonyms: Mage-l2; NDNL1; nM15; ns7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224146 representing NM_013779
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCAGCTAAGTACGAATCTGGGGGATTCCAGCCCTCCGGAGTCCCCAGTGCCTGCAGTCCATAGCC
  GCCCTACGGTTCTGATGCGGGCTCCGCCTGCTTCCTCCCGGGCTCCTCCTGTCCCCTGGGATCCACCTCC
  AGTGGACTTGCAGGCTCCCATGGCTGCTTGGCAGGCTCCTCAACCTGCCTGGGAGGCTCCGGAGGGCCAG
  CTGCCTGCCCCAGTGGCTCAGCTGGCCCAGCCTCCTGGTCTAGGGGCCCCAATGGTCCAGGCTCCACCGC
  TCGGAGGGGGGATGGCCAAGCCTCCAACTCCTGGAGTCTTGATGGTCCATCAGCCCCCTCCGGGAGCCCC
  CATGGCCCAGTCTTCAACTCCGGGAGTCCTGATGCTACATCCTTCTGTCACGGGGGCCCCTTTGGCTCAT
  CCTCCTCCCCCAGGAACCCCGATGACACACCCTCCCGGGACCTCGATGGCGCACCCTCCTCCTCCTCCTC
  CTCCCCCTCCTCCTCCTCCTCCTCCTGGGACCCCAATGACCCACCCACCTCCTCCTGGGACTCCGATGGG
  TCACCATCCTCCTCCTGGGAACCCTATGACCCATCCTCCACCGGGGAACCCGATGGTGCATCCTCTCACT
  CATGGAGCCCCGATGGTCCATGGGGGACCACATGGAACTCCAATGCCGCATGTTCCCATTACGGGGACAC
  CGATAGCCCAGCAGCCAACTCCAGGAGTCCTGATGGCCCAGCAGCTGACACCGGGAGTCCTGATGGTCCA
  GCCGCCTGCTCCGGGAGCTCCGATGGTCCAGCCACCTCCACAGGCTGCCTTGATGACCCAGCCTGCACCT
  TCGATTACTCCGATGGCCAAGCCTCCAGGTCCTGGTGTCGTGATGATCCATCCTCCAGGTGCCAGAGGTC
  CAATCATTCAGACTCCAGTATCAGGAGCACCAATGGCTCAGACAGTGCTGCCCCCTGGGCAGCCTCTGGC
  CACTTGGGCCCCACAGGGTCAGCCTTTGATCCTACAAATCCAGTCTCAAGTCATAAGGGCTCCTCCACAG
  GTTCCCTCTGTACCACAAGCCCCCCAGGTACAGCTGGCCACACCCCCAGGCTGGCAAGCCACCACACCCA
  ACTGGCAGGTGACCCCCCAGGGGTGGCCAGCAACGCCTTTGACATGGCAGGCTACACAGGTGACCTGGCA
  GGCCCCCACAATAGCCTGGCAGGCCACTCAACCTGGGCGCCAAGGGCACTCAACCATTCGTACTGGTCAC
  ACACCCATTCGACCTGGACCAGCTCCATTGCTTCGCCAGATACCTCCTATGATCCGTCAGATCCAACCTG
  TGATGAGGCAAGCCCCACCACTGATCCGACAGGTCCCTATCAGACCAGCTCCACATGGCATAGCAAGCCA
  GCCTCAGCTGTGGCAGGTCCTGCCACCCCCACCTCCACTGCGGCAGGCTCCACAGGCTCGTCTACTGCTC
  CCGAGGGTACCAGGAACAGGCCAGGTGTCTACAGTACCACCAGTTGCTCAGATACATTTGGTGCCACAGT
  CAGGCCCACAGGTGCCCCAGACAGTACTGCCAGCCCAACTGTCTATCCCAATTCCTGTACCCCAGGCTGC
  TGCTCAGTCTGCTCCCCGGACTGTGCATTGCCCACCCATCATCTGGCAGGCCCCCAAAGGCCAGGCCCCG
  GTGCCACAGGAGCTCCCAGTGCCACAGGAGCTCCCGGTGCCACAGGAGCTCCCGGTGCCACAAGAGGTCC
  CGGTTCCACAGGAGATCCCGGTGCCACAGGAGATCCCGGTGCCACAGGAGCTCCCGGTTCCACAGGAGCT
  CCCGGTGCCACAGGAGCTCCCAGTGCCACAAGAGCTCCCGGTGCCACAGGAGCTCCCAGTGCCACAGGAG
  CTCCCGGTGCCACAGGAGCTCCCGGTGCCACAGGAGCTCCCGGTGCCATTGGAGTTCCAGGAGGTACAGC
  AGGCCCAGGCAGTGGGCTGGCGGGCACCCAAGGTACCTCCTCACTTCTGGCAGCCTGTGTCTGCCCAGGA
  GGCCCAGGAGCAGGCCACTCAGATCGCCCATGTGGAGCAGCAGCAGCCCTTTCAGGGAGCTCCAGCCTCC
  TCCAAGGCACTGCAAACTCAGCTGCCGACCCACCAGGCCCAAGCCTCTGGCTTGCAGGCAGAACTGCCTT
  CAGTGCAGCTGCAGCCTTCTTGGCAAGGCCCACTGCCCATGTTGCAGGCCCAGCCTGGAGCCTCTGCCAC
  ACTGGCAAACTTTCCCCGGGGCTCCACTCGATCACGTATGGCTCCATCAGGAGAACCTGGCCCCTCTTCT
  CTAGAACCTCGGGGCCCTCCTAGGGAACGTAGGGCACCTGCAAGGGACAAAAAGGGTCCTCCAAAAGAGC
  GCATGTTCATTGGTGCCACTTTCTGTGCTCCAAGGGGGGCATCAGCATCCAGGGCATACGTGCCAACTGC
  CTGGAAAAACTTGCCTGCCACATCAGAGACCTTTCCTGCCACCTCAAGGGTCTTTCCATCTACCTCTCAT
  TTCCAGCCTGCCTCTTCTAATGCCTTTAGAGGTCCATCTGCCGCCTCAGAGAGCCCAAAGTCACTGCCAT
  TTGCTCTGCAGGATCCTTATGCCTGCGTAGAGGCCCTGCCTGCAGTTCCCTGGGTTCCGTATCCAGATGG
  AAATGCCTCATCAGCATGTAAGTCAGTGCCTGCCATCTTGATGGTGGCAGCAGCTGCCCCCCAGGCAAGT
  GCCACTGCTGCAGAGGCCTCTAAGTCTTCAGAGCCGCCAAGACGCCCCGGCAAAGCCACCAGGAAGAAGA
  AGCATCTGGAACCCAAAGAAGACAACTGTGGCCACAGGCTCTCCTCACGTGACTGGCGGGGGCCCCGAAC
  CTGGGGCAATCCCAGTCATTCTGACTGGGAGATTCAGAGGGCTATGCAGCTCCTGGGGGACCGGGAATCC
  CTCTACACTCCGCAGGGCCTGAATGACTGGGGGTGCCCCAACACTTCTAGGATGCCAAGGAGCTTGGAGG
  GCCCTAGCACTTCACGGGACCAGGAATTCTGTGGTGACTCGGGTGGGTCTCAGACATGGATGGCTTCTGA
  GGTCCCAAGCGTCTCTCGGGGATCCAGTGCTGCTCAGGAGGACCCTGATAGGGAGAGTCAGCCCTTATCT
  CCCTTAGATGAGAGAGCAAACGCTTTGGTGCAGTTTCTCTTGGTCAAAGACCAAGCCAAGGTGCCTGTCC
  AGCTCTCGGAGATGGTAAATGTTGTCATCCGAGAATACAAAGACGACAGCTTAGACATCATCAACCGTGC
  CAACACTAAGCTGGAGTGCACCTTTGGTTGTCAACTGAAGGAAGTTGACACCAAAACCCACACTTACATC
  ATCGTCAACAAGATGGCGTACCCTCAGTGTAATTTGCTGGCATCCTATTTAGAGAGGCCAAAGTTCAGCC
  TCCTGATGGTGGTCTTGAGCCTCATCTTTATGAAAGGCTACTGTATCAGGGAGAATCTGCTCTTTAGTTT
  TCTGTTCCAGCTAGGGCTGGATGTCCAGGAGACAAGTGGTCTCTTCAGAATTACAAAGAAGCTCATCACC
  AGTGTGTTTGTGAGACACAGGTACCTAGAGTACAGGCAAATCCCGTTCACTGAGCCTGCAGAATACGAGC
  TTCTCTGGGGGCCCCGGGCATTCCTCGAAACCAACAGGGTGCACATCTTGAGATTTTTGGCCGCACTCTA
  CGAGAACCAGCCCCAGATCTGGTCATGCCAGTACCTTGACTCACTGGCAGAGTTAGAATACAAGGACGCA
  AATGCCGCCGCCGAAGAGTCCCATGACAGCGATGATGATGCCCACGACCCCACCAGCAGTCCCCATCCTC
  ACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013779
• Insert Size: 3855 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013779.2, NP_038807.4
• RefSeq Size: 4662 bp
• RefSeq ORF: 3855 bp
• Locus ID: 27385
• UniProt ID: Q9QZ04
• Cytogenetics: 7 34.37 cM
• Gene Summary: Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (PubMed:22208286).[UniProtKB/Swiss-Prot Function]