Dennd3 (NM_001081066) Mouse Untagged Clone

CAT#: MC224136

Dennd3 (untagged) - Mouse DENN/MADD domain containing 3 (Dennd3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dennd3
• Synonyms: AI447457; E030003N15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224136 representing NM_001081066
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCCGCGGCGCGACACCTGTCGCTGCCCTCGGGGCTGCTGGAGTTGTGCGCGCTGCTGGGCG
  CCTCTCAAGACAGCCTCCGCGGCCTGGAGCAGATTGCCCAGAAACGAGGAGTGAAGAGCGCCTCTTCCCT
  TGTCCCCGAAGTTCTGTCTGTTTTCGTGCCTCCTTTCACCACAAAAGAGGATGGCCAGGTACCGGGGGCC
  AGCTGTGCTCTTGGCAAAGGCAGGAGGCGTTCCTTCCGGAAGAAGCGAGAGAAGCCCAGGATGGAGCCCT
  GGAAGAGCCACCCTGGAGACTCCAAGGGGCCCGATTCTGAAGATGTCACCATCCCAGGAGGTGTGGACCT
  CCTTGCCCTGCCTCAGCTCTGCTTCCCAGGGTGTGTGTGTGTGGCTTCGGAACCCAAAGAGGACTACATC
  CATTTCCTGGTGCTCACGGATGTCTGTGGAAATAGAACCTATGGTGTGGTCGCCCAGTACTATCGACCCC
  TGCATGATGAATATTGTTTCTACAATGGCAAGAGCCACTGGGAGCCCAGCGTGATCTCAGCTAGGTGCTT
  TGTGCCTTTTGCAGTGTGTGTGGTGTCCCGATTCCCCTACTATAACTCCCTCAAGGACTGCCTCTCCTGT
  TTATTGACTCATCTGAAGCTCTGTAAAGATTTTGAAGTTGACAATCACATAAAGGATTTCGCTGCACGAC
  TGTCCTTAATACCTAGTCCACCGCCTGGGCCGCTCCACTTGATATTTAACATGAAGCCGCTGCAGGTCGT
  CTTTCCCTCTCGAGCGGACCCCGAGAGCCCCATCGTCGACTTGGACCTTCACCTGCCCCTGCTGTGCTTC
  CGGCCTGAGAAAGTGTTGCAGATCCTGACCTGTATCTTGACGGAACAGCGCATCGTGTTCTTCTCCTCAG
  ACTGGGCCCTGCTGACGCTCATGGCCGAGTGCTTTGTGGCCTACCTCCACCCGCTGCAGTGGCAGCACAC
  GTTTGTGCCCATCCTGTCGGGTCAGATGCTGGACTTCGTCATGGCTCCCACGTCTTTCCTCATGGGCTGT
  CATCTCGACCACTTTGAAGAAGTCAGAAAGGAAGCTGATGGTTTGGTCCTGATTGACATTGACCACGGGA
  GTGTCACCTGCTCCAAGTCTTCTGACGACAACATAGACATTCCTGATGTCCCCCTTCTGCTGGCACAGAC
  CTTCATTCAGAGAGTCCAGAGTCTCCAGCTGCACCCGGATTTGCACCTTGCCCACCTGAGTGCCAGCACA
  GACTTGAACGAGGGCCGAGCCCGCCGCCGAGCCTGGCAGCAGACTCTCAACTGCAAGATTCAGCACATCA
  CTCTACAGCTGCTCGTTGGCATCTTCAGGGAAGTGAAGAATCACTTGAATTATGAACACCGCGTCTTCAA
  CAGCGAGGAGTTTCTCAAGACAAGAGCGGCAGGGGACCAGCAGTTTTATAAGCAGGTTTTGGACACCTAC
  ATGTTCCACTCCTTTCTGAAGGCGCGGCTCAACGGCAGGATGGACGCCTTTGCTCGGATGGACCTCGACA
  CTCAGTCTGAGGAGGACAGGATAGACAGGATGCTGATAAGTCCAAGGAGACCTACTGTGGAGAAGATGGC
  CTCCAGGAAGGCCTCACCCCTGCACATCACCCACAGACGCATGGTGGTCAGCATGCCCAACCTGCAGGAC
  ATCTCCCTGCCCGAGCTGCCTCCCAGGAACTCATCGCTTCGGATAATGGACACCTCAAACTGCAGAAGCA
  GCAGTCCAGTTCTCAAAGTGACCCCTAAGTCAACATATATGTTCAAGATCCCTGATATCCACTTCCCACT
  GGAGAGCCAGTGTGTTCAGGCATACTATACAGACTTCGTCACTCTCCTGAGCAAGGCCATGGCGCTGTTG
  GGTCCTGGCGACTCTCTGCTCCTAGCCAGGTACTTCTACCTGCGTGGTCTTCTCCATCTGATGCAGGGAC
  AACTGCTGAGTGCCCTCTTGGATTTCCAGAACCTGTATAAGACCGACATAGGGATCTTCCCGGCTGATCT
  GGTCAAGAGAACGGTGGAATCCATGTCGGCCTCTGAGCGGGCCCAGGCCGAGCGGACGCCTGAGCTCAGG
  CGGCTCATCACAGAGGTCTTTGATAAGCACGGCGAGGCCCCCAAGGCAGATGACGCCGTGAAGAACTTCG
  AACTGCCCAAGAAGCACATGCAACTCAATGACTTTGTGAAGCGAGTCCAGGAGTCGGGGATCGTGAAGGA
  CGCCGTCATTATCCACCGTCTGTTTGATGCACTGACCTTCGGACACGAGAAGCAGATTGACCCGGAGACC
  TTCCGAGACTTTTACACCTGCTGGAAGGAGACTGAGGCAGAGGCACAGGAGGTCAGCCTGCCCGCACTGC
  TCATGGAGCACCTGGATAAGAACGAGTGCGTCTACAAGCTGTCGAGCTCTGTGAAGACCAACCGTGGAGT
  GGGCAAGATCGCCATGACCCAGAAGCGCCTGTTCTTGCTCACCGAGGGGCGGCCCGGCTATGTGGAGATT
  GCCACCTTCAGGAACATAGAGGAAGTCAAAAATAGCACCGTGGCATTTCTCCTCCTGAGGATACCCACCT
  TAAAGATCAAGACGGTGGCCAAGAAGGAAGTCTTCGAGGCCAACCTCAAGTCGGAGTGTGACCTCTGGCA
  CCTCATGGTGAAGGAGATGTGGGCCGGAAAGCAGCTGGCAGATGACCACAAGGACCCACAGTACGTCCAG
  CAGGCACTGACCAATGTCCTGCTCATGGATGCCGTGGTTGGCACGCTGCAGTCACCTAGTGCCATTCATG
  CTGCCTCCAAGCTGGCCTACTTTGACAACATGAAGAAGAAGTCACCCATGGCGGTCCCAAAGACAACCTC
  GGAGACCCTAAAACACAAGATTAACCCTTCAGCCGGAGAGACCGCTCCCCAAGCCATTGAAGTCCTGCTG
  TATACTCCAGGGCGCCTGGATCCTGCAGAGAAGGTGGAAGATGCCCACCCCAAGCTGTGGTGTGCCCTGA
  ACGAAGGCAAAGTGGTCGTGTTTGATGCCTCCTCTTGGACCGTCCACCAGCATTGCTTTAAAGTGGGTTC
  CTCGAAAGTGAACTGTATGGTGATGGCGGAGCACAACCAGGTGTGGGTGGGCTCCGAGGACTCTGTCATC
  TACATCATCAATGTCCACAGCATGTCCTGCAACAAACAGCTCACGGATCACCGTTCTCCAGTCACTGGCT
  TGGCCGTGCACAATGGAAAGAAGCCCAGTGAGATCTACTCCTGTAGTCTGGATGGGACGGTCATCGCGTG
  GAATGTCAGCACACTGCGAGTGATCAGCCGGTTCCAGCTGTCATACGGTGACCTCCTGTCCATCAGCCTG
  CACAACGACCGCATATGGTGTTGTACAGTCCACAAGATCTTGGTGGTGACCCCGCAGGGCTTTGTTCGTC
  AAGAGCTGAAGCATCCGAAAGATGCCTCCTTCCTGGCCTTCCAACTTCTGCCTGAGGAGCAGCAGCTGTG
  GGCGGCCTCTACGGGGGTCAGTGAGCTGTATATGTGGAGCCTGAAGGACCTGGACCAGCCTCCTCAGAAG
  ACTTACTTACAAGACTGCTCCGAGGTCACCTGTATGATCCGGGTGAAGAGGCAGATCTGGGTGGGCGGCA
  GGGGGCTGTCTCAGGGAAAAACCAGAGGGAAGATCTACGTGATGGACGTGGAGAAGGTGACGGTGGAAAA
  GGAGCTGGTGGCACACTTGGACACCGTGAGGACCCTGTGTTCTGCTGAGGACCGCTACGTACTGAGCGGA
  GCGGGCCAGGAGGAGGGAAAGATCGCCATCTGGAAGGTGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081066
• Insert Size: 3825 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081066.1, NP_001074535.1
• RefSeq Size: 5299 bp
• RefSeq ORF: 3825 bp
• Locus ID: 105841
• UniProt ID: A2RT67
• Cytogenetics: 15 D3
• Gene Summary: Guanine nucleotide exchange factor (GEF) activating Rab12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab12 into its active GTP-bound form. Regulates autophagy in response to starvation through Rab12 activation (PubMed:24719330, PubMed:25925668, PubMed:28249939). Starvation leads to ULK1/2-dependent phosphorylation of Ser-554 and Ser-572, which in turn allows recruitment of 14-3-3 adapter proteins and leads to up-regulation of GEF activity towards Rab12 (PubMed:25925668). Also plays a role in protein transport from recycling endosomes to lysosomes, regulating, for instance, the degradation of the transferrin receptor and of the amino acid transporter PAT4 (PubMed:21718402, PubMed:24719330). Starvation also induces phosphorylation at Tyr-940, which leads to up-regulated GEF activity and initiates autophagy (PubMed:28249939).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the protein coding transcript. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.