Nid1 (NM_010917) Mouse Untagged Clone

CAT#: MC224059

Nid1 (untagged) - Mouse nidogen 1 (Nid1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nid1
• Synonyms: A630025O17; entactin; entactin-1; Nid; nidogen-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224059 representing NM_010917
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACGCGAGCGGCTGTAGTTGGGCGATGTGGACGTGGGCGCTGTTGCAGCTGCTGCTACTAGTGG
  GGCCCGGAGGCTGCCTGAATCGCCAGGAGCTCTTCCCCTTCGGCCCTGGGCAGGGGGACCTAGAGCTGGA
  GGCCGGGGACGACGTGGTATCCCCCTCCCTGGAACTGATCGGAGAACTGAGCTTCTATGATCGTACGGAC
  ATCACGTCGGTCTATGTCACCACAAATGGTATCATCGCCATGAGCGAACCCCCAGCCACAGAATACCATC
  CCGGAACCTTCCCACCCAGCTTCGGCTCAGTAGCGCCTTTCCTGGCTGACTTGGACACAACCGATGGCCT
  GGGGAATGTGTATTATCGAGAAGACTTATCCCCGTTCATTATTCAGATGGCAGCAGAGTATGTCCAGAGA
  GGCTTCCCGGAGGTCTCTTTCCAGCCCACTAGTGTGGTGGTTGTCACTTGGGAATCTGTGGCCCCTTATG
  GAGGGCCCAGCAGCAGCCCTGCCGAGGAAGGCAAGAGAAACACGTTCCAGGCTGTTCTGGCCTCCTCAAA
  TTCCAGCTCCTATGCCATTTTCCTTTATCCTGAAGATGGTCTACAGTTCTTTACAACATTCTCGAAGAAG
  GATGAAAGCCAAGTACCTGCTGTGGTTGGTTTCAGCAAAGGTCTAGTAGGATTTCTATGGAAGAGCAACG
  GAGCCTATAACATATTTGCCAATGACAGAGAATCAATTGAAAATTTGGCTAAGAGCAGCAACGCTGGGCA
  CCAGGGTGTGTGGGTGTTTGAGATTGGAAGTCCTGCTACCGCCAAGGGCGTGGTGTCTGCAGATGTGAAC
  CTGGACCTGGACGACGATGGAGCAGACTATGAAGATGAAGATTATGACCTAGTAACCTCTCACCTTGGCC
  TGGAGGATGTAGCCACTCCCTCCCCCTCCCACAGTCCAAGAAGGGGATACCCTGACCCACACAATGTACC
  TAGGATCCTCTCTCCTGGCTATGAGGCTACAGAGAGACCCCGTGGAGTCCCCACTGAGAGGACCAGATCT
  TTCCAGCTGCCAGCGGAGAGGTTCCCTCAGCATCACCCCCAGGTCATTGATGTGGATGAAGTAGAGGAAA
  CAGGAGTTGTATTCAGCTACAACACAGGTTCCCAGCAGACTTGTGCCAACAATAGACACCAGTGCTCCGT
  GCATGCAGAGTGCAGAGACTATGCTACTGGCTTCTGCTGCAGGTGTGTGGCCAACTACACAGGCAATGGC
  AGACAGTGCGTGGCAGAAGGCTCTCCACAACGGGTCAATGGCAAGGTGAAGGGAAGGATCTTCGTGGGGA
  GCAGCCAGGTCCCCGTGGTGTTTGAGAACACTGACCTGCACTCCTATGTGGTGATGAACCACGGGCGCTC
  TTACACAGCCATCAGCACCATCCCTGAAACCGTCGGCTACTCTCTGCTCCCCCTGGCACCCATTGGAGGC
  ATCATCGGATGGATGTTTGCAGTGGAGCAGGATGGGTTCAAGAATGGGTTTAGCATCACTGGGGGCGAGT
  TTACCCGGCAAGCTGAGGTGACCTTCCTGGGGCACCCAGGCAAGCTGGTCCTGAAGCAGCAGTTCAGCGG
  TATTGATGAACATGGACACCTGACCATCAGCACGGAGCTGGAGGGCCGCGTGCCGCAGATCCCCTATGGA
  GCCTCGGTGCACATTGAGCCCTACACCGAACTGTACCACTACTCCAGCTCAGTGATCACTTCCTCCTCCA
  CCCGGGAGTACACGGTGATGGAGCCTGATCAGGACGGCGCTGCACCCTCACACACCCATATTTACCAGTG
  GCGTCAGACCATCACCTTCCAGGAGTGTGCCCACGATGACGCCAGGCCAGCCCTGCCCAGCACCCAGCAG
  CTCTCTGTGGACAGCGTGTTTGTCCTGTACAACAAGGAGGAGAGGATCTTGCGCTATGCCCTCAGCAACT
  CCATCGGGCCTGTGAGGGATGGCTCCCCTGATGCCCTTCAGAATCCATGCTACATTGGCACCCATGGGTG
  TGACAGCAATGCTGCCTGTCGCCCTGGCCCTGGAACACAGTTCACCTGCGAATGCTCCATCGGCTTCCGA
  GGAGACGGGCAGACTTGCTATGATATTGATGAGTGTTCAGAGCAGCCTTCCCGCTGTGGGAACCATGCGG
  TCTGCAACAACCTCCCAGGAACCTTCCGCTGCGAGTGTGTAGAGGGCTACCACTTCTCAGACAGGGGAAC
  ATGTGTGGCTGCCGAGGACCAACGTCCCATCAACTACTGTGAAACTGGTCTCCACAACTGTGATATCCCC
  CAGCGAGCCCAGTGCATCTATATGGGTGGTTCCTCCTACACCTGCTCCTGTCTGCCTGGCTTCTCTGGGG
  ATGGCAGAGCCTGCCGAGACGTGGATGAATGCCAGCACAGCCGATGTCACCCCGATGCCTTCTGCTACAA
  CACACCAGGCTCTTTCACATGTCAGTGCAAGCCTGGCTATCAGGGGGATGGCTTCCGATGCATGCCCGGA
  GAGGTGAGCAAAACCCGGTGTCAACTGGAACGAGAGCACATCCTTGGAGCAGCCGGCGGGGCAGATGCAC
  AGCGGCCCACCCTGCAGGGGATGTTTGTACCTCAGTGTGATGAATATGGACACTATGTACCCACCCAGTG
  TCACCACAGCACTGGCTACTGCTGGTGTGTGGACCGAGATGGTCGGGAGCTGGAGGGTAGCCGTACCCCA
  CCTGGGATGAGGCCCCCGTGTCTGAGTACAGTGGCTCCTCCTATTCACCAGGGACCAGTAGTACCTACAG
  CTGTCATCCCCCTGCCTCCAGGGACACACTTACTCTTTGCTCAGACTGGAAAGATTGAACGCCTGCCCCT
  GGAGAGAAACACCATGAAGAAGACAGAAGCCAAGGCCTTTCTCCATATCCCTGCAAAAGTCATCATTGGA
  CTGGCCTTTGACTGCGTGGACAAGGTGGTTTACTGGACAGACATCAGCGAGCCTTCCATTGGGAGAGCCA
  GCCTCCACGGTGGAGAGCCAACCACCATCATTCGACAAGATCTTGGAAGCCCTGAAGGCATTGCCCTTGA
  CCATCTTGGTCGAACCATCTTCTGGACGGACTCTCAGTTGGATCGAATAGAAGTTGCAAAGATGGATGGC
  ACCCAGCGCCGAGTGCTGTTTGACACGGGTTTGGTGAATCCCAGAGGCATTGTGACAGACCCCGTAAGAG
  GGAACCTTTATTGGACAGATTGGAACAGAGACAATCCCAAAATTGAGACTTCTCACATGGATGGCACCAA
  CCGGAGGATTCTCGCACAGGACAACCTGGGCTTGCCCAATGGTCTGACCTTTGATGCATTCTCATCTCAG
  CTTTGCTGGGTGGATGCAGGCACCCATAGGGCAGAATGCCTGAACCCAGCTCAGCCCGGCAGACGCAAAG
  TTCTCGAAGGGCTCCAGTATCCTTTCGCTGTGACTAGCTATGGGAAGAATTTGTACTACACAGACTGGAA
  GACGAATTCAGTGATTGCCATGGACCTTGCTATATCCAAAGAGATGGATACCTTCCACCCACACAAGCAG
  ACCCGGCTATATGGCATCACCATCGCCCTGTCCCAGTGTCCCCAAGGCCACAATTACTGCTCAGTGAATA
  ATGGTGGATGTACCCACCTCTGCTTGCCCACTCCAGGGAGCAGGACCTGCCGATGTCCTGACAACACCCT
  GGGAGTTGACTGCATTGAACGGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010917
• Insert Size: 3738 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010917.2, NP_035047.2
• RefSeq Size: 6046 bp
• RefSeq ORF: 3738 bp
• Locus ID: 18073
• UniProt ID: P10493
• Cytogenetics: 13 5.26 cM
• Gene Summary: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.[UniProtKB/Swiss-Prot Function]