Sec31a (NM_026969) Mouse Untagged Clone

CAT#: MC224013

Sec31a (untagged) - Mouse Sec31 homolog A (S. cerevisiae) (Sec31a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sec31a
• Synonyms: 1810024J13Rik; ABP125; ABP130; AU042160; HSPC275; Sec31l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224013 representing NM_026969
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTAAAGGAGATAGATCGTACAGCTATGCAGGCATGGAGCCCTGCCCAGAACCACCCCATTTACC
  TAGCCACAGGAACATCCGCTCAGCAGTTGGATGCGACGTTTAGTACCAATGCTTCCCTCGAGATATTCGA
  GCTGGACCTTTCGGACCCATCCTTGGATATGAAGTCTTGTGCTACGTTCTCCTCTTCTCACAGGTACCAC
  AAGTTGATCTGGGGACCTCATAAGATGGATTCCAAAGGAGATGTTTCTGGAGTTCTGATTGCAGGTGGAG
  AAAATGGAAACATTATTCTTTATGATCCTTCTAAAATTATAGCTGGAGACAAGGAAGTTGTGATTGCCCA
  AAAGGACAAGCATACTGGGCCAGTGAGAGCCTTGGATGTGAACATCTTCCAGACCAATTTGGTGGCCTCT
  GGTGCTAATGAATCTGAAATCTACATTTGGGATCTAAACAATTTTGCAACTCCAATGACGCCGGGAGCTA
  AAACACAGCCACCAGAAGATATCAGCTGCATAGCATGGAACAGACAAGTTCAGCATATCTTAGCATCAGC
  CAGTCCCAGTGGCCGGGCAACTGTCTGGGATCTCAGGAAAAACGAGCCTATCATCAAAGTCAGTGACCAC
  AGTAACAGGATGCATTGCTCTGGGCTGGCGTGGCACCCTGATGTGGCTACTCAGATGGTCCTCGCCTCTG
  AGGATGATCGATTACCAGTGATCCAGATGTGGGACCTTCGATTTGCCTCCTCTCCTCTCCGGGTTCTGGA
  AAATCATGCCAGGGGGATTTTGGCAGTTGCTTGGAGCATGGCAGATCCTGAGTTGTTGTTAAGCTGTGGA
  AAAGATGCTAAGATTCTCTGCTCTAACCCTAACACAGGAGAGGTATTATATGAACTTCCCACCAACACAC
  AGTGGTGCTTTGATATCCAGTGGTGTCCTCGAAATCCTGCTGTCCTGTCCGCTGCTTCCTTTGATGGACG
  GATCAGTGTCTATTCCATCATGGGAGGGAGCATAGACGGCTTACGGCAGAAACAAGTTGACAAGCTTTCA
  TCATCTTTTGGGAATCTTGATCCTTTCGGCACAGGACAGCCTCTCCCTCCATTACAAATCCCACAGCAGA
  GCGCTCAGCACAGCATAGTGCTGCCCCTGAAGAAGCCTCCCAAGTGGATCCGGAGGCCTGTGGGCGCTTC
  CTTCTCGTTTGGAGGGAAGTTGGTGACTTTTGAGAGCGTTGCAGTGCCGCTGCAGCAGGGAGCTGAGCAG
  CAGCGACGGCAGCCTGTGTTCATCAGTCAGGTGGTGACGGAGAAGGACTTTCTCAACCGGTCGGCGCAGC
  TGCAGCATGCTGTGCAGTCTCAGGGCTTCATCGGTTACTGCCAGAAAAAGATCGAGGCTTCCCAGACCGA
  GTTTGAGAAAAATGTGTGGTCCTTCTTGAAGGTAAACTTTGAGGAGGATTCTCGTGGAAAATACCTTGAG
  CTTCTGGGATACAGAAAGGAAGATCTGGGGCAGAAGATTGCTTTGGCCTTGAACAAAGTGGACGGACCCG
  ATGTGGCTCTTAAGGACTCTGACCAAGTAGCGCAGAGTGATGGGGAGGAGAGTCCTGCTGCTGAAGAGCA
  GCTCTTGGGAGAGCGCATTAAAGAGGAAAAACAGGAATGTGACTTTCTGCCCTCGGCGGGAGGGACGTTT
  AACATCTCTGTCAGTGGGGATATTGACGGTCTAATTACCCGGGCCTTGCTGACAGGCAATTTTGAGAGTG
  CAGTTGACCTTTGCTTACATGATAACCGAATGGCTGATGCCATTATATTAGCCATAGCAGGTGGACAGGA
  ACTCTTGGCTCAAACACAAAAGAAATACTTCGCAAAATCCCAAAGCAAAATTACCAGGCTTATCACTGCA
  GTGGTGATGAAGAACTGGAGGGAGATTGTTGAATCCTGTGATCTTAAGAATTGGAGAGAGGCTTTAGCTG
  CAGTCCTCACTTACGCTAAACCAGACGAGTTCTCGGCCCTGTGCGATCTCCTGGGAACCCGTCTGGAAAG
  AGAAGGAGACAGCCTCCTGCGGACTCAGGCCTGCCTCTGCTACATCTGTGCAGGGAATGTAGAGAGATTG
  GTTGCTTGCTGGACAAAAGCTCAGGACGGCAGCAGCCCCTTGTCTCTCCAGGATCTGATTGAGAAAGTTG
  TCATCCTCCGCAAAGCTGTACAGCTCACCCAAGCCTTGGACACCAATACTGTCGGGGCTCTTCTGGCTGA
  GAAGATGAGTCAGTATGCCAGCTTGTTGGCAGCCCAGGGCAGCATTGCCGCAGCCTTGGCTTTTCTGCCT
  GACAACACCAACCAGCCAAATATTGTGCAGCTGCGTGACAGACTCTGTAAAGCTCAAGGCAAGCCTGTGT
  CGGGACAGGAATCTTCTCAGAGCCCATATGAGAGGCAGCCACTGTCCAAGGGCAGGCCTGGACCAGTTGC
  TGGCCACTCGCAGATGCCCAGAGTTCAAACTCAGCAATATTATCCCCATGGAGAAAATCCCCCACCTCCA
  GGTTTCATAATGCAGGGCAATGTCATTCCAAACCCTGCTGCCCCGCTGCCCACGGCTCCAGGCCACATGC
  CCAGCCAGCTGCCACCTTACCCACAGCCGCAGCCTTACCAGCCAGCCCAGCAGTATTCCTTCGGAACAGG
  GGGGGCAGCAGCTTATCGACCTCAACAGCCTGTTGCTCCTCCTGCTTCTAACGCTTACCCTAACACCCCG
  TACATATCTCCTGTGGCTTCCTATTCTGGGCAGCCTCAGATGTACACAGCCCAGCAAGCCTCCTCACCTA
  CCTCCAGCTCTGCTGCTTCTTTCCCCCCTCCCTCCTCTGGAGCATCCTTCCAGCATGGCGGACCAGGAGC
  TCCACCATCATCTTCAGCTTATGCACTGCCTCCTGGAACAACAGGTACACCGCCTGCTGCCAGTGAGCTG
  CCTGCGTCCCAAAGAACAGAAAATCAATCTTTCCAAGACCAGGCATCTATATTGGAAGGTCCTCAGAATG
  GTTGGAATGATCCTCCAGCTCTGAACAGAGTGCCGAAGAAGAAGAAGATGCCTGAAAACTTCATGCCTCC
  TGTTCCCATTACGTCACCAATCATGAACCCTTCGGGTGATCCCCAGTCACAGGGGCTGCAGCAGCAGCCT
  TCAACTCCAGGGCCGCTGTCAAGCCACGCCTCCTTCCCACAGCAGCACCTAGCGGGCGGCCAGCCCTTCC
  ATGGTGTACAGCAGCCTCTTGCTCAGACAGGCATGCCGCCGTCCTTCTCAAAGCCCAACACTGAAGGGGC
  TCCAGGAGCTCCCATTGGAAACACCATCCAGCACGTGCAGGCTCTGCCAACAGAAAAGATTACTAAGAAA
  CCTATTCCGGAGGAGCACCTCATCCTAAAGACGACATTTGAGGACCTTATCCAGCGCTGCCTCTCCTCCG
  CTACAGACCCCCAAACCAAGAGGAAGCTGGACGACGCCAGCAAGCGCCTGGAGTTCCTCTACGACAAGCT
  TAGGGAACAGACACTCTCCCCGACCATCATCAACGGTCTCCACAGCATCGCCAGGAGCATTGAGACGAGG
  AACTACTCGGAAGGGTTGAGCGTCCACACTCACATCGTCAGCACCAGCAACTTCAGTGAGACCTCGGCCT
  TCATGCCCGTCCTCAAGGTTGTCCTCAGTCAGGCCAGCAAGCTGGGTGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026969
• Insert Size: 3693 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026969.1, NP_081245.1
• RefSeq Size: 4244 bp
• RefSeq ORF: 3693 bp
• Locus ID: 69162
• UniProt ID: Q3UPL0
• Cytogenetics: 5 E4
• Gene Summary: Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (By similarity). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks an alternate in-frame exon, contains an alternate in-frame exon, and uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.