Chl1 (NM_007697) Mouse Untagged Clone

CAT#: MC223938

Chl1 (untagged) - Mouse cell adhesion molecule with homology to L1CAM (Chl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chl1
• Synonyms: A530023M13Rik; AI465420; CALL; LICAM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223938 representing NM_007697
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAATTGCCATTATGTGGAAGAGGACTGATCCTAAGTCTAATTTTCCTCCTGTTAAAATTGTCAG
  CAGCTGAAATACCACTCTCCGTTCAACAGGTTCCAACAATTGTAAAGCAGTCATACGTGCAGGTGGCCTT
  TCCCTTCGATGAGTATTTTCAAATTGAATGTGAAGCGAAAGGAAACCCTGAACCAATATTTTCATGGACT
  AAGGACGATAAGCCTTTTGATCTTTCTGATCCTCGGATAATTGCGGCTAACAATTCAGGCACATTCAAGA
  TCCCAAATGAGGGCCACATCTCTCACTTTCAAGGAAAATACCGCTGTTTCGCATCCAATAGACTAGGAAC
  TGCAGTATCAGAAGAAATAGAGTTCATAGTACCAGGGGTTCCAAAATTCCCAAAAGAAAAAATTGAACCC
  ATTGATGTAGAAGAAGGGGATTCCATTGTCTTACCCTGCAACCCTCCAAAAGGCCTCCCACCTTTGCACA
  TCTATTGGATGAATATTGAGTTAGAACACATTGAACAAGATGAGAGAGTATACATGAGCCAAAGAGGAGA
  TCTGTACTTTGCAAATGTGGAAGAAAATGACAGTCGGAATGATTACTGTTGCTTCGCTGCATTTCCAAAA
  TTAAGAACGATTGTACAGAAAATGCCAATGAAACTGACTGTGAACAGTTCAAATTCCATCAAGCAAAGGA
  AACCCAAACTTCTGTTGCCTCCTGCCCAAATGGGAAGTTTATCTGCAAAGACTGTACTCAAAGGGGATAC
  ACTATTGCTCGAGTGTTTTGCGGAGGGCCTACCAACTCCACATATCCAGTGGAGCAAACCAGGTAGTGAA
  TTACCAGAGGGAAGAGCAACAATTGAAGTTCATGAAAAGACATTGAAGATAGAAAACATCTCCTACCAGG
  ATAGAGGAAATTATCGCTGTACAGCCAACAATTTGTTGGGGAAAGCCAGTCATGATTTTCACGTCACAGT
  AGAAGAGCCTCCACGCTGGAAAAAGAAGCCTCAGAGTGCCGTGTACAGCACGGGGAGCAGTGGCATCTTG
  TTGTGTGAAGCAGAAGGCGAACCTCAACCCACAATCAAATGGAGACTCAATGGCTTGCCGATTGAAAAAC
  ATCCATTTCCTGGTGACTTTATGTTCCCCAGGGAAATCAGTTTTACAAACTTACTGCCAAATCATACTGG
  TGTATATCAGTGTGAAGCCTCAAATATTCACGGAACCATTCTGGCCAATGCCAATATTGACGTTATAGAT
  GTTATCCCACTGATAAAAACTAAGAATGAAGAAAACTACGCGACAGTGGTTGGCTACAGCGCTTTCTTAC
  ACTGCGAGTACTTTGCTTCTCCCAAGGCCACCGTGGTCTGGGAAGTGGCTGATGAGACACATCCCCTGGA
  AGGTGATCGGTACCATACGCATGAAAACGGCACACTGGAGATCTACAGAACTACTGAGGAGGATGCGGGG
  TCCTACTCTTGTTGGGTAGACAATGCCATGGGAAAAGCCGTCATCACAGCGAACTTGGATATTAGAAATG
  CTACAAAGCTCAGAGTTTCTCCTAAGAATCCTCGAATCCCTAAATCACATGTGCTTGAACTATACTGCGA
  AAGCCAGTGTGACTCACATTTGAAACACAGTTTGAAGTTGTCCTGGAGTAAAGATGGAGAAGCTTTTGAG
  ATGAATGGCACAGAAGATGGCAGAATAGTGATTGACGGAGCTTACCTGACCATATCTAACATCACAGCAG
  AAGATCAGGGTGTTTACTCCTGTTCTGCTCAAACTTCTCTTGATAGTACTTCGGAAAAAACTCAAGTAAC
  CGTCCTTGGTGTTCCAGATCCGCCAGGAAACCTTCACTTGTCAGAAAGACAAAACAGAAGTGTCCGGCTG
  TCCTGGGAAGCAGGAGATGACCACAACAGCAAAATCAGCGAGTACATTGTAGAATTTGAAGGGAACAGAG
  AGGAGCCTGGAAAGTGGGAGGAGCTAACTAGAGTCCAGGGAGAAGAAACAGATGTCGTGTTATCTTTGGC
  TCCTTATGTGAGATATCAGTTTAGGGTCACAGCTGTAAACGAAGTCGGAAGAAGCCACGCCAGCCTGCCT
  TCAGACCATCATGAAACTCCACCAGCAGCTCCAGACAAGAATCCCCAGAACATCAGAGTTCAAGCATCTC
  AACCCAAGGAAATGATTATAAAATGGGAGCCTTTGAAATCCATGGAACAGAATGGGCCAGGACTGGAGTA
  CAAAGTGAGCTGGAAGCCCCAGGGAGCCCCCGAAGAGTGGGAAGAAGAAATAGTTACAAACCACACACTA
  CGTGTGATGACACCTACTGTCTATGCTCCTTATGATGTCAAGGTCCAAGCCATCAATCAACTAGGCTCCA
  GCCCTGACCCTCAGCCAGTGACACTCTATTCAGGGGAAGACTATCCTAGTACAGCCCCTGTGATCCAGCG
  TGTGGATGTTATGAACAGTACATTAGTTAAAGTTACCTGGTCATCCATTCCTAAGGAAACAGTACACGGA
  CTTCTAAGAGGGTACCAGATAAATTGGTGGAAAACCAAAAGCCTGTTGGATGGAAGGACACATCCCAAAG
  AAGTAAACATTCTAAGGTTTTCAGGACAGCGAAACTCTGGAATGGTTCCTTCCCTAGACCCCTTTAGTGA
  ATTTCACTTAACAGTCTTAGCCTATAACTCCAAAGGAGCTGGTCCAGAGAGCGAGCCTTATATATTTCAG
  ACACCAGAAGGAGTACCTGAACAACCAAGTTTTCTCAAGGTCATCAAAGTTGATAAAGACACTGCCACCT
  TATCCTGGGGCCTTCCTAAGAAACTAAATGGAAATTTAACTGGTTATCTCTTACAATACCAGATAATAAA
  TGACACCTACGAGCTTGGAGAGTTAAATGAAATCAACGTTACTACTCCATCTAAATCCAGCTGGCATCTC
  TCCAACCTCAACTCAACAACAAAATACAAATTCTACCTGAGGGCGTGCACCTCAAGAGGCTGTGGAAAGC
  CAATAAGTGAAGAAGGTGCCACCCTAGGAGAAGGGAGTAAAGGTATCAGGAAGATAACAGAAGGAGTAAA
  TGTTACCCAAAAGATTCACCCTGTAGAGGTACTTGTGCCGGGAGCGGAACATATTGTTCACCTAATGACT
  AAGAATTGGGGTGATAACGATAGCATTTTTCAAGATGTAATTGAGACAAGAGGGAGAGAATATGCTGGCT
  TATATGATGACATCTCCACTCAAGGCTGGTTTATTGGACTGATGTGTGCAATTGCTCTTCTTACACTGAT
  ATTGTTAACTATTTGCTTTGTGAAGAGGAACAGAGGTGGAAAGTATTCAGTAAAAGAAAAGGAAGATTTA
  CACCCAGATCCAGAAGTTCAGTCAGCAAAAGATGAAACTTTTGGTGAATACAGCGACAGTGATGAAAAAC
  CTCTCAAGGGAAGCCTTCGGTCCCTGAATAGGAACATGCAGCCCACAGAGAGTGCTGACAGTTTAGTGGA
  ATATGGAGAGGGAGACCAGTCCATATTCAACGAAGATGGATCGTTTATTGGCGCATACACTGGAGCTAAG
  GAGAAGGGATCCGTTGAAAGCAATGGAAGTTCAACTGCCACCTTCCCACTCCGGGCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007697
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007697.2, NP_031723.2
• RefSeq Size: 7751 bp
• RefSeq ORF: 3630 bp
• Locus ID: 12661
• UniProt ID: P70232
• Cytogenetics: 6 E1
• Gene Summary: Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane.[UniProtKB/Swiss-Prot Function]