Dgkk (NM_177914) Mouse Untagged Clone

CAT#: MC223610

Dgkk (untagged) - Mouse diacylglycerol kinase kappa (Dgkk), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dgkk
• Synonyms: Gm360
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223610 representing NM_177914
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGGCCCCAGAGTCCTGGCCAGAGCCAACTCCAGAGTCCTGTCTAAGAATGAGTCCAGCACTAC
  ATCTGTTGCAGGTAGTTCCTGGAGAGAAAAGTGGAACAGCATCTCCAGTGCTATTGCCATTGCCGTCGCC
  CAAACCGCCAATAACGTGGTCCAGAATAAAGAAAATACTGAAGGAAGGACCCCTGCTGAAGAACTGTAAC
  TCCTTCAGAAGATGGAAGCTTAGATACTGTCTGGTTCAAGGGCAGAAGCTCCACTTTGCACACCATCCTT
  CGTTTGCACACTTTGAAACAATTGATCTGTCTCAAGTTGTTTTGGCTGAAAGTAGCTGCAGAAATCTTTG
  CCATGGTTTTTGTGTTATCACACCACATCGAAAAGTGTCTCTAGTTGCACCCACTCGGCAAGACATGGAA
  GAATGGATTAACATCATAAAAACTGTCCAACAAGGAGAAATCCGTCAGATCCCTGCAGCCGAAAACAACC
  CCTTTCTTGTCGGAATGCACTACTGGTATTCCAGTAGTAATCCCCGGAGTCACTTCTGCAATGTTTGTCG
  AGAGAACATTCCTGCTTTATCTCGAGATGCCGTCACCTGTGAAGTCTGTCAGGTGAAATCTCATAAATTC
  TGTGCTTTGAGAGCAAACAAAGACTGTAAATGGAATACTTTATCCGTGACTGATGACCTTCTTCTACCCG
  CTGATGAAATACAAACAATGCCGCATCAGTGGGTAGAAGGAAATATACCCGCTGGCTCTCAATGTGTCGT
  ATGTCACAAGAGCTGCGGTAGTCATCACAGACTTCAGGATTTCCGCTGCCTCTGGTGCGGCTCTACGGTG
  CACGGGGCCTGCCAGAAGCGGTTTTCCAAGGAATGTTCCTTCGGAAGTCGTCGCTCGTCCATCGTGCCAC
  CAACGGCACTGAGCGACCCTAGGGGCGATGGCCAGCTAGTAGTATCGCCTGACTTCTGGAATCTTGACTG
  GCCACTGACTTGTTCCTGTCCCTTGCTCATCTTCATCAACTCTAAAAGTGGAGATCATCAAGGGATCATC
  TTTCTCCGGAAATTCAAACAATACCTTAATCCGTCTCAAGTATTCGACCTGGCGAAGGGTGGACCTGAAG
  CAGGCATCGCTATGTTCAAGAACTTTGCCCGTTTTCGTGTTCTGGTTTGTGGTGGAGATGGCAGTGTGAG
  CTGGGTCTTGTCTACGATTGATGCCTATGGATTGCATGATAGGTGTCAGCTGGCGATCATCCCACTTGGA
  ACTGGTAATGATCTCGCTCGTGTCCTGGGCTGGGGAGCAGTCTGGAGTAAAGGCACATCACCACTGGATA
  TTCTCAGCAGAGTGGAGCAGGCTCACGTGAGGATTTTAGACAGATGGAGTGTGATGATCCGTGAGACCCC
  CAGACAAGCCCCGCTGCTGAAAGGACAAGTGGCAATGGATATACCAAGATTTGAGGCTGCTGCCATCAAG
  AACGTAGAGTCTGCAACCACTGAGTTGAACAAAATTCTGAAGGCCAAATATCCCACGGAGATGGTCATCG
  CCACAAGATTCCTGTGCTCAGCAGTGGAAGATTTTGTGGAAGATATTGTAAAGGCCTGGCACCAAATAAA
  ACAGAACAGCACAGCAGTGGAGTCTGTGATTTTGAAAAGCGACTTAATGTATGATAAACTCAGTGTCCTT
  ATTGATCTCCTGGCTGAGGATGCAGTAGCTGCCTCCGCTGAGAGGACAGCCACAGCATATGGAAGCAGAA
  GCCAAGCAGATGGAAAACCCTTCGTGCCTCAACTAGACCACATAGCAAGGGCCAAACTGGAGTTGGCTGA
  AAGGGCTCAGAAACTCCAGCAGTCCTTGAAACTCATCATATTCCAGGTCGAACAAGTTCTGGATGAAGAA
  AGCAGACAGAGCTTATCAGTTAAGAACTTTACTTCATCTTTGTTCCTGGGAGACGGAGACGACGACGACT
  CAGATGACTATGATCAGAGCCCTAGACACCGTTCTCGTTGTGACATCTTATGTTCTATACCTTCTCTGAG
  AAATGAAGACCTAGATAACCTTGACTTAGAACACTTACACATTGCACCTGAAACGATACGCTTCAAAGAA
  AAGTGTGTCATGAACAACTACTTTGGAATTGGACTAGACGCAAAAATCTCTCTGGAGTTTAATTCCAGAA
  GAGAAGAACATCCAGAACAATACAATAGCCGCCTCAAGAACAAAATATGGTATGGTCTTCTGGGAAGCAA
  GGAATTACTGCAACGCTCTTACAGGAAATTGGAAGAGCGAATACACCTCGAGTGTGATGGAGAAGCTGTC
  TCCTTGCCAAACCTTCAAGGCATCGTAGTGCTCAACATTACGAGCTACGCTGGAGGCGTCAACTTTTGGG
  GAAGAAACAGAGCAACCACAGAATATGATGTCCCTGCAATCAATGATGGGAAGCTAGAGGTTGTGGCAAT
  CTTTGGTTCTGTGCAGATGGCCATGTCTCGTATCGTCAACCTGCAACAACATCGAATTGCGCAGTGCCAT
  GAGGTGGTAATAACTATTGATGGTGAAGACGGTGTCCCTGTGCAAGTGGATGGGGAAGCCTGGATTCAGA
  AGCCAGGCCTTATCAAGATTAAATACAAGAATGTTGCCCAGATGTTGATGAGAGATAGGGACTTTGAGAA
  CTCAATGAAAACCTGGGAATCTAAGCATACTGAAATCCAAGCTGTCCAACCACCCCACCTGGATTTCCAG
  GAATCTCAAGACAGCCTCTCTGATGGAGAGTATGCCCAGATGCAGCACTTGGCTCGGCTTGCAGAAAACC
  TCATTAGCAGACTTACTGACCTAAGCAAGGTCCACCAGCACGTGTCTGTCCTCATGGATTCTGTGAATGC
  CAGTGCTAACATACTGAATGATGTGTTCTATAGCCAAGACAGTGGTAACGAAGCAGGCGCAGCTTCCTGC
  ATTCCCATTGAGACTCTAAGCAGAACTGATGCAGTAGATGTTACATTTAGTCTTAAAGGGCTCTACGATG
  ACACCAAAGCTTTCCTGGATGAAAACTTGTTGAGAGATGCTGAGGATCGGGCCATGCTACAAACTGCCTT
  GGATGCCATGAATACAGAGTTAAGAAGGATACTGGCAATTGGCTGGCTGAGTCAAATCTTCTTTCCAGAG
  GAACAGGCTTCTGACACTCGCAGTTTAAGTCGTAGGTTTAGAATAAAATTCCCCAAGCTGGGGAAGAAGA
  AACAACGAGAAGAGGGAGAAAAGCCTAAATCAAGCCAAAGATTCCCTGGTTTTCTTGGCAAGTTCTGGCG
  TCGAAGAAATCGTAGCAATCGAGCAAAAGCTGATGACCCTCCAACACCTTCAAGTTCTCAACTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177914
• Insert Size: 3357 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177914.3, NP_808582.3
• RefSeq Size: 7172 bp
• RefSeq ORF: 3357 bp
• Locus ID: 331374
• UniProt ID: E9Q7N4
• Cytogenetics: X A1.1