Ube3c (NM_133907) Mouse Untagged Clone

CAT#: MC223487

Ube3c (untagged) - Mouse ubiquitin protein ligase E3C (Ube3c), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ube3c
• Synonyms: AI853514; mKIAA0010
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223487 representing NM_133907
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGCTTCGAAGGCGACTTCAAGACAAGGCCCAAAGTGTCTCTCGGTGGCGCGAGCAGGAAGGAAG
  AAAAGGCTTCTCTTTTACATCGCACTCAGGAAGAAAGACGCAAGAGAGAGGAAGAAAGGCGAAGATTGAA
  AAATGCAGTAATAATCCAATCTTTTATCCGAGGCTATAGAGACAGAAAACAACAATACTTCATCCAAAGA
  AGCGCGTTTGACCAATGCACTGACTCGGCTCAGCCTGGTGGTACCTTCTGCCTTGCTGATGGTCCCAACC
  TTACCCTCTTGGTAAGGCAGCTTCTGTTCTTTTACAAGCAGAGTGAAGACTCCAAACGTTTGATATGGCT
  TTATCAGAACTTAATTAAACACAGCTCTCTGTTTGTCAAGCAATTGGATGGATCAGAGAGACTGACATGC
  TTATTTCAAATAAAGAGGTTGATGAGCCTCTGTTGCAGGTTGCTGCAAAACTGTAGTGATGACAGTCTGA
  ATGTTGCACTTCCAATGCGAATGCTTGAGGTGTTTACATCTGAGAATACATACTTGCCTGTTTTACAAGA
  TTCTAGCTATGTGGTGTCAGTGATTGAACAAATTTTGCACTACATGGTTCACAGTGGATATTATAGATCT
  CTCTACTTGTTGATTAATAGCAAGCTTCCATCAAGTATTGAATATTCTGATTTATCTCGAGTTCCTATAG
  CAAAAATTTTGCTGGAGAATGTCCTAAAACCATTGCACTTTACGTATAGCTCCTGTCCTGAAGCCTCAAG
  GCATCAAGTTTTCTCAGCCTTCACAGAGGAGTTTCTGGGAGCACCTTTTACCGATCAAATTTTTCATTTC
  GTCATTCCGGCCTTTGCAGATGCACAAACTGTGTTCCCTTATGAGCCCTTTCTCAATGCATTGTTGTTAC
  TTGAGAGTCAAAGTTCAAAACGGTGTAGTGGAGTGCCATGGCTCTTCTATTTTGTTTTAACTGTTGGCGA
  GAATTATTTAGGAGCACTCTCTGAGGATGGTCTACTGGTTTATCTGCGAGTGCTTCAGACCTTCCTCTCT
  CAGCTACCTGCTTCACCCACTGGTACAGGCTGTCCTGATTCCACCAGTGACTCTGAGGACGATAACGAGG
  AGACTGACCAACCCAACAGCCCTGAGGATGGCAGGGTGTCAGCCCCATATATTACTGAAGAGTGTCTGAG
  AAAACTGGATACGAAGCAGCAAACCAACACCCTGCTGAACCTGGTGTGGAGGGACTCGGCCAGTGAGGAG
  GTCTTCACAAGGATGGCTTCTATCTGCCACACATTGATGGTGCAGCACCGAATGATGGTGCCCAAAGTCA
  GGCTTCTCTACAGTTTAGCCTTCAATGCCAGGTTTTTGAGGCATCTTTGGTTTCTCATCTCTTCAATGAC
  AACACAGATGATCACAGGATCTATGGTGCCATTGCTTCAGTTGATATCGCGGGGTTCGCCCATGTCTTTT
  GAAGATTCTAGTCGAATCATCCCACTCTTTTACCTCTTCAGCTCCTTGTTCAGTCATTCGCTGATCTCCA
  TACATGACAATGAATTTTTTGGTGACCCCATAGAAGTTGTAGGTCAGAGACAATCATCCATGATGCCTTT
  CACTCTGGAGGAGTTAATCCTGCTGTCACGATGCCTTCGAGATGCCTGCTTGGGGATCATCAAGCTAGCA
  TACCCGGAAACAAAGCCAGAAGTTCGGGAAGAATATGTCACCGCATTTCAGAGTATTGGGGTCACTACTA
  ATTCTGAGATGCAGCAGTGCATACAAATGGAACAGAAGCGATGGGTTCAGCTGTTCAAGGTTATCACCAA
  CCTAGTGAAAATGCTGAAGTCTAGAGACACGAGGAGAAATTTCTGTCCTCCAAACCACTGGCTGTCAGAA
  CAAGAGGATATCAAAGCAGATAAAGTTACCCAGCTCTATGTACCTGCATCTAGACATGTGTGGCGATTCC
  GGAGGATGGGCAGGATTGGCCCACTGCAGTCCACCCTGGAGGTGGGTCTGGAGTCCTTACCCCTGTCTGT
  GTCTGAGGAACGACAGCTTGCCATCCTGACAGAACTGCCTTTTGTGGTTCCATTTGAGGAGCGAGTGAAG
  ATCTTTCAAAGGTTGATTTATGCTGATAAGCAAGAAGTTCAAGGCGATGGTCCATTTCTGGATGGAATCA
  ATGTTACTATAAGGAGAAATTATATTTATGAAGATGCTTATGACAAACTTTCTCCAGAAAATGAGCCTGA
  TTTGAAAAAACGGATCCGGGTGCACTTGCTCAATGCCCATGGCCTGGATGAAGCTGGCATCGATGGTGGT
  GGTATTTTCAGAGAGTTTTTAAATGAACTACTGAAATCTGGATTTAACCCCAACCAGGGCTTCTTTAAGA
  CTACTAATGAAGGGCTTCTATACCCCAACCCAGCTGCTCAGATGCTTGTGGGAGATTCTTTCGCAAGGCA
  TTACTACTTCCTAGGCAGAATGCTTGGAAAGGCTCTGTATGAAAACATGTTGGTGGAGCTACCCTTTGCT
  GGCTTTTTCCTGTCCAAGCTACTGGGAACCAGTGCAGATGTGGACATTCACCATCTTGCCTCATTGGACC
  CCGAAGTCTACAGGAACCTGCTTTTCCTCAAGAGCTATGAGGAGGATGTGGAGGAACTGGGACTGAACTT
  TACTGTGGTGAACAATGACCTGGGAGAAGCCCAGGTGGTTGAACTAAAATTTGGTGGAAAAGACATCCCG
  GTAACTGGGGCCAACCGAATTGCCTACATCCACCTGGTGGCTGACTACCGGCTGAACAAGCAGATCCGAC
  CGCATTGCCTAGCTTTCCGCCAGGGCCTGGCCAATGTTGTGAGCCTAGAGTGGCTCCGAATGTTTGACCA
  ACAGGAAATTCAGGTATTAATTTCTGGTGCACAAGTTCCTGTAAGTCTGGAGGACCTAAAATCCTTTACA
  AACTATTCAGGGGGCTATTCTGCCGATCATCCTGTCATTAAAATCTTTTGGAGAGTTGTAGAAGGTTTCA
  CTGACGAAGAAAAGCGCAAGTTGCTCAAGTTTGTAACAAGTTGCTCTCGCCCCCCTCTCCTGGGTTTCAA
  GGAGCTATACCCTGCGTTTTGCATTCACAATGGAGGCTCTGACCTTGAGCGGCTACCTACAGCCAGCACC
  TGCATGAACCTGCTGAAGCTCCCAGAGTTTTATGATGAAGCCCTGCTGCGAAGCAAGCTGCTGTATGCCA
  TTGAGTGTGCTGCTGGCTTTGAGCTGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133907
• Insert Size: 3252 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133907.3, NP_598668.1
• RefSeq Size: 5033 bp
• RefSeq ORF: 3252 bp
• Locus ID: 100763
• UniProt ID: Q80U95
• Cytogenetics: 5 B1
• Gene Summary: E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.[UniProtKB/Swiss-Prot Function]