Supt16 (NM_033618) Mouse Untagged Clone

CAT#: MC223373

Supt16 (untagged) - Mouse suppressor of Ty 16 homolog (S. cerevisiae) (Supt16h), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Supt16
• Synonyms: Cdc68; Fact140; Spt16; Supt16h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223373 representing NM_033618
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGACTCTGGACAAAGACGCGTACTACCGGCGAGTGAAGAGATTGTACAGTAACTGGCGGAAAG
  GAGAAGATGAGTATGCCAGTATTGATGCCATTGTTGTATCGGTGGGTGTTGATGAAGAAATTGTGTATGC
  CAAGTCAACTGCCTTACAGACGTGGCTCTTTGGTTATGAACTAACTGATACAATCATGGTCTTCTGTGAT
  GACAAAATCATCTTCATGGCCAGCAAAAAGAAGGTGGAGTTTCTGAAACAGATTGCCAATACTAAAGGCA
  ATGAGAATGCTAATGGAGCCCCTGCCATCACACTGCTTGTCAGAGAGAAGAATGAAAGTAACAAGAGCAG
  CTTTGACAAAATGATTGACGCTATCAAAGAAAGCAAGAGCGGCAAGAAGATCGGAGTGTTCAGCAAAGAC
  AAGTTCCCTGGAGAGTTCATGAAGAGCTGGAGTGATTGTCTCAACAAGGAGGGCTTTGACAAAGTAGACA
  TCAGCGCTGTTGTGGCATACACCATTGCTGTGAAAGAGGACGGTGAGCTCAACCTGATGAAGAAAGCAGC
  CAGCATCACCTCTGAGGTCTTCAACAAGTTCTTTAAGGAAAGAGTCATGGAAATAGTGGATGCAGATGAG
  AAAGTTCGGCATAGCAAATTGGCTGAGTCTGTGGAAAAGGCCATTGAAGAAAAAAAATACCTAGCTGGGG
  CAGATCCTTCTACTGTGGAAATGTGTTACCCTCCTATCATTCAGAGTGGTGGCAACTATAATCTCAAGTT
  CAGTGTGGTGAGTGATAAGAATCATATGCATTTTGGGGCCATTACGTGTGCCATGGGCATTCGCTTTAAA
  TCTTACTGCTCCAACCTTGTTCGCACTCTGATGGTTGACCCCACTCAGGAAGTTCAAGAAAATTACAACT
  TTTTACTTCAACTTCAAGAGGAGTTGCTAAAGGAATTAAGACATGGTGTGAAGATATGTGATGTGTATAA
  CTCTGTCATGGATGTGGTTAAGAAGCAGAAACCAGAATTGCTGAACAAAATTACAAAGAATCTAGGATTT
  GGGATGGGAATTGAATTCCGTGAAGGCTCTCTAGTAATCAATAGTAAAAATCAGTACAAGCTAAAGAAAG
  GGATGGTTTTTAGCATCAATCTAGGATTTTCAGACCTGACTAACAAAGAAGGGAAAAAGCCAGAAGAGAA
  AACCTATGCCCTGTTCATTGGTGACACGGTGCTTGTAGATGAGGATGGTCCAGCCACTATTCTTACTTCT
  GTGAAAAAGAAAGTAAAGAATGTGGGGATTTTCCTGAAGAATGAGGATGATGAAGAGGAGGAGGAAGAGA
  AAGATGAGGCAGAGGACCTTTTGGGAAGAGGCTCTAGGGCGGCATTACTTACTGAAAGAACAAGGAATGA
  GATGACTGCAGAAGAGAAGCGAAGAGCACATCAGAAGGAACTGGCAGCACAGCTCAACGAGGAAGCGAAG
  AGGAGGCTGACAGAGCAGAAAGGGGAACAGCAGATTCAGAAAGCTCGAAAATCTAATGTGTCCTATAAAA
  ACCCATCTCTGATGCCTAAGGAACCACATATTCGGGAGATGAAGATCTATATTGATAAGAAATATGAGAC
  TGTGATAATGCCTGTGTTTGGCATTGCCACACCCTTCCATATTGCCACAATCAAGAACATAAGTATGTCT
  GTCGAAGGAGACTATACTTACTTGCGAATCAACTTCTATTGTCCAGGCAGTGCTCTGGGCAGGAATGAGG
  GCAACATCTTTCCTAACCCTGAAGCCACTTTTGTCAAGGAAATTACATACCGAGCTTCAAATATGAAAGC
  ACCTGGAGAGCAGACTGTACCCGCCTTAAATCTTCAGAATGCGTTCCGAATTATAAAAGAAGTACAAAAA
  CGTTACAAGACCAGAGAAGCTGAAGAGAAAGAAAAAGAGGGCATTGTAAAACAAGACTCGCTGGTGATCA
  ACCTAAACCGGAGTAATCCAAAACTGAAAGACCTGTACATTCGTCCAAACATTGCCCAGAAGAGAATGCA
  GGGCTCACTGGAGGCTCATGTCAATGGTTTCCGATTCACATCTGTTCGAGGAGACAAGGTGGATATTCTG
  TACAATAATATCAAGCATGCGCTGTTCCAGCCCTGTGATGGCGAGATGATTATTGTTTTGCACTTTCACC
  TCAAGAATGCTATCATGTTTGGGAAGAAGCGACACACAGATGTACAGTTCTACACAGAAGTTGGAGAGAT
  CACTACAGATTTGGGGAAACATCAACACATGCATGACCGAGATGACCTGTATGCTGAGCAGATGGAACGA
  GAAATGAGACACAAACTGAAAACGGCCTTTAAAAATTTCATTGAAAAAGTAGAGGCCCTAACAAAGGAGG
  AGCTGGAGTTTGAAGTGCCATTTAGGGACCTGGGGTTTAATGGGGCTCCCTACAGGAGTACCTGCCTCCT
  TCAGCCCACTAGTAGTGCCCTGGTAAATGCTACAGAGTGGCCACCCTTTGTGGTGACACTGGATGAAGTG
  GAGCTGATCCATTTTGAGAGGGTCCAGTTTCACCTGAAGAACTTTGATATGGTCATTGTCTACAAGGATT
  ACAGCAAGAAAGTCACGATGATCAATGCTATTCCTGTTGCCTCTCTAGACCCCATCAAGGAGTGGCTGAA
  TTCCTGTGACCTAAAGTACACAGAAGGAGTTCAGTCTCTCAACTGGACTAAAATCATGAAGACCATTGTT
  GATGACCCCGAGGGCTTCTTTGAACAAGGTGGCTGGTCCTTCCTGGAACCTGAGGGTGAGGGGAGTGACG
  CTGAGGACGGAGACTCGGAGTCTGAAATTGAAGATGAGACTTTCAATCCTTCTGAAGATGACTATGAAGA
  GGAAGAAGAGGACAGCGATGAAGATTACTCATCAGAAGCTGAAGAATCAGACTATTCTAAGGAATCTCTG
  GGAAGTGAAGAAGAAAGCGGGAAGGACTGGGATGAGCTAGAAGAAGAAGCACGAAAAGCGGACCGGGAAA
  GCCGTTATGAGGAGGAAGAAGAACAGAGCCGAAGTATGAGCCGGAAGAGGAAGGCATCTGTACATAGTTC
  AGGCCGTGGCTCTAACCGTGGTTCCAGACACAGCTCTGCACCCCCGAAGAAAAAGAGAAAGTAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_033618
• Insert Size: 3144 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033618.3, NP_291096.2
• RefSeq Size: 4491 bp
• RefSeq ORF: 3144 bp
• Locus ID: 114741
• UniProt ID: Q920B9
• Cytogenetics: 14 26.83 cM
• Gene Summary: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).[UniProtKB/Swiss-Prot Function]