Nlrc4 (NM_001033367) Mouse Untagged Clone

CAT#: MC223257

Nlrc4 (untagged) - Mouse NLR family, CARD domain containing 4 (Nlrc4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nlrc4
• Synonyms: 9530011P19Rik; Card12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; IPAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223257 representing NM_001033367
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTATAAGGAACAACAGACGAGCCCTTATTCAAAGGATGGGCTTAACAGTTACCAAGCAAATCT
  GCGATGACCTCTTTGCATTGAACGTTCTCAACAATCAAGAAGCTAATGTCATTTACTGTGAGCCCTTGGA
  GCAGGAAGCCGCCCGAAAGATCATCCATATGACTATGCAGAAGGGCTCAGCGGCCTGCAACCTCTTTCTT
  AAGAGTCTTGAAAACTGGGACTATTTTGTGTATCAGGACTTAACTGGACAAAATCTTTCTTATCAGGTCA
  CAGAAGAAGACCTGAATGTTTTGGCCCAGAATTTAAAGGACTTGTACAACAGCCCTGCTTTTCTGAACTT
  CTACCCCCTGGGTGAAGATATCGACATAATTTTTAATCTGGAGAAAACCTTCACAGAACCTATCATGTGG
  AAGAAGGACCATCGTCATCACCGTGTGGAGCAGCTGACTTTGGGCAGCCTGCTCGAGGCTCTGAAGAGCC
  CCTGCCTGATTGAAGGCGAGTCTGGCAAAGGGAAGTCCACCCTGCTGCAGAGAATCGCTATGCTCTGGGC
  CTCTGGGGGCTGCAGGGCTCTGAAGGGGTTCAGATTAGTCTTCTTCATCCACCTGAGAAGCGCCAGGGGG
  GGACTATTCGAAACACTGTACGATCAGCTCCTGAACATACCCGACTTCATCAGCAAGCCGACCTTCAAGG
  CTCTGCTGCTGAAGCTACACAAGGAGGTCCTCTTTCTTCTCGATGGTTACAATGAATTCCATCCCCAGAA
  CTGCCCAGAAATTGAAGCCCTGATAAAGGAAAACCATCGCTTCAAGAACATGGTCATTGTCACCACCACC
  ACGGAGTGCCTGAGGCATATCAGACATGTTGGCGCCCTGACTGCGGAGGTGGGAGATATGACCGAAGACA
  GTGCCAAAGATCTCATCGAGGCAGTGCTGGTACCTGATCAGGTTGAACGCCTGTGGGCCCAAATCCAGGA
  GTCCAGGTGCCTGAGAAATCTGATGAAGACCCCTCTCTTCGTGGTGATCACCTGTGCAATTCAGATGGGC
  AGACAGGAATTCCAAGCTCACACCCAAACCATGCTGTTCCAAACCTTCTACGACCTCCTGATACAGAAAA
  ACAGCCACAGATATAGAGGTGGAGCTTCAGGTGATTTTGCCAGGAGCCTAGACTACTGTGGAGACCTGGC
  CCTAGAAGGTGTGTTCGCCCACAAATTTGATTTTGAACCCGAGCATGGGTCCAGCATGAACGAGGACGTC
  CTGGTGACAATAGGGCTCCTCTGTAAGTACACAGCTCAGAGGCTGAAGCCCACGTATAAATTCTTTCATA
  AATCATTTCAGGAGTACACGGCAGGTCGGAGACTCAGCAGTTTGCTGACGTCCAAAGAGCCAGAGGAGGT
  GAGCAAAGGGAACAGCTACTTAAACAAAATGGTTTCCATCTCTGACATCACATCCCTATATGGCAATCTG
  CTCCTCTACACGTGTGGGTCGTCCACAGAAGCAACCAGGGCGGTCATGAGGCACCTTGCAATGGTTTATC
  AGCACGGCAGCCTACAAGGACTTTCAGTCACCAAGAGGCCTCTCTGGAGGCAGGAATCAATCCAGAGTCT
  GAGAAATACCACTGAGCAAGATGTTCTGAAAGCCATCAATGTAAATTCCTTCGTAGAGTGTGGCATCAAT
  TTGTTCTCAGAGAGTATGTCTAAATCAGACCTGAGCCAAGAATTTGAAGCTTTCTTTCAAGGTAAAAGTT
  TATACATCAACTCAGAGAACATCCCTGACTATTTATTTGACTTCTTTGAATACTTGCCTAATTGTGCAAG
  CGCATTGGACTTCGTGAAGTTGGATTTCTATGAAAGAGCTACAGAGTCACAGGACAAGGCAGAAGAGAAT
  GTCCCTGGAGTTCACACAGAAGGGCCCTCAGAAACCTACATTCCCCCCAGGGCTGTGTCTTTGTTCTTCA
  ACTGGAAGCAGGAATTCAAGACTCTAGAGGTCACACTCCGAGATATTAACAAGTTGAATAAGCAAGATAT
  CAAATATCTGGGGAAGATATTCAGCTCTGCCACCAACCTCCGGCTGCATATCAAGAGATGTGCAGCCATG
  GCTGGAAGACTCAGCTCAGTCCTCAGAACCTGCAAGAACATGCATACCCTCATGGTGGAAGCCAGTCCCC
  TCACCACGGATGACGAACAGTACATCACATCTGTGACAGGCCTCCAGAACTTAAGTATTCACCGCTTGCA
  CACTCAACAGCTGCCAGGTGGTCTGATTGACAGCTTGGGTAATCTGAAGAACCTCGAGAGACTCATACTG
  GATGACATCAGGATGAACGAGGAAGATGCTAAAAACCTAGCCGAAGGCCTACGAAGCCTGAAGAAGATGC
  GTTTACTCCATTTGACTCATTTGTCTGACATTGGGGAGGGGATGGACTACATAGTCAAGTCTCTCTCAGA
  AGAATCCTGTGATCTCCAAGAGATGAAGTTGGTGGCCTGCTGTCTGACTGCAAACTCTGTGAAAGTTCTA
  GCACAGAATCTTCACAATTTGATCAAGCTGAGCATTCTTGATATATCAGAAAATTACCTGGAAAAGGATG
  GGAATGAAGCTCTACAGGAACTGATCGGCAGGCTTGGCGTTCTGGGAGAGCTCACTACATTGATGCTGCC
  TTGGTGCTGGGATGTGCACACCAGCCTGCCCAAGCTGTTGAAGCAGTTGGAGGGGACCCCAGGACTTGCC
  AAACTTGGATTGAAAAACTGGAGACTCAGAGACGAAGAGATTAAAAGTTTAGGTGAATTTCTGGAGATGA
  ATCCTCTGAGAGACTTGCAGCAGTTGGATTTAGCGGGGCACTGTGTGAGCAGTGACGGATGGCTTTACTT
  CATGAATGTGTTTGAGAATCTGAAGCAGTTAGTGTTTTTTGACTTTAGCACTGAGGAGTTCTTACCGGAT
  GCAGCACTGGTGAGGAAACTTAGTCAAGTGTTATCCAAGTTAACTCTTCTGCAAGAGGTAAAGCTCACGG
  GCTGGGAGTTTGATGACTATGATATTAGCGCTATTAAAGGCACCTTTAAACTAGTGACTGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033367
• Insert Size: 3075 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033367.3, NP_001028539.1
• RefSeq Size: 3838 bp
• RefSeq ORF: 3075 bp
• Locus ID: 268973
• UniProt ID: Q3UP24
• Cytogenetics: 17 E2
• Gene Summary: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7.[UniProtKB/Swiss-Prot Function]