Tubgcp5 (NM_146190) Mouse Untagged Clone

CAT#: MC223253

Tubgcp5 (untagged) - Mouse tubulin, gamma complex associated protein 5 (Tubgcp5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tubgcp5
• Synonyms: B130010C12Rik; GCP5; mKIAA1899
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223253 representing NM_146190
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGCCTAAGTCGTCCGGGAGCCGGATGGACAGACAGCTGGAGCATGATGTGTGCGAGCTCGTCC
  GGCAGGTTACGGGGCTGCAGGACGAGGCGGACCCCAACTTCCAGCTGGCCCTGGACTTCGTCTGGTCCAA
  CTTCAGATTTCATCGTTTCTTGGATGTCAACAGTCACAAAGTAGAAAAAACAATAGAAGGAATTTATGAA
  AAATTCACCATTCATTCTGATCTCAACAAAGCTGCTAGTTGGAAGAGATTAACTAAGGAGTTCCTAAATG
  CATCTCTTCCCAGCACAGAGAAAATAAAGACAGATGCACATTATTCCATACTGTCACTCCTTCTGTGTCT
  GTCTGATTCTCCTTCAAACAGCAATTATGTGGAAACACCGAGAGAGAAAGAAGTGGAAAAGAAGGATGAT
  TTTGACTGGGGGAAGTACCTGATGGAAGGTGAAGAAATTGGCCTTGGTCCAAATATTGATACGCCGAATT
  GGTCTGAAGACAGTGATGATGAAGATGCACAGCAGCCCTTGAGCAGAGAAGACTCTGGGATACAGGTAGA
  CAGGACCCCATTAGAAGAGCAAGACCACAACAGAAAAGGGGGGCCTCAAGTCTGCTGGGAAGATGAGCCA
  GATAGCCGGAGCTGGCTGGAACAGCATGTAGTTCATCAGTACTGGACAACCAGGCGCTTCCGAATTCCAC
  ACAGTGCACATCTGCACTCCAATTTAGCTGCTGTCTGGGATCAACACCTGTACAGCAGTGACCCTCTGTA
  CGTGCCAGATGACAGGGTGGTGGTTACTGAGACACAGGTCATTCGGGAGACCCTGTGGTTACTTTCAGGG
  GTGAAAAAGATGTTTATATTTCAGTTGATAGATGGGAAGGTAACTGTGAGAAACAATATTATTGTAACTC
  ACTTGACACATAGCTGTTTACGATCTGTGCTGGAACAAATAGCAGCGTATGGCCAGGTTGTGTTTCGGCT
  CCAGGAGTTCATTGATGAAGTCATGGGGCACAGCTCTGAAAGTCTACCACCGGGAAATGGCCCCATACCT
  AAGAAGCAACCTGACGCTCCCTTTAGAACCTACCAGGCCTTCATGTGGGCCCTCTACAAATACTTCATTA
  ACTTCAAGGAAGAGCTTACAGACATTGAGAAGTGTGTTATCAGTAGTGATACAACCATAACACTTGCAAT
  AGTGGTGAACAAGTTGGCACCTCGATTGGCGCAGCTAAAGGTCCTGGACAAAGTGTTTAGTACTGGAGTG
  GCAGAAGTTCCACCTGATACTCGCAATGTCGTCCGGGCATCTCACCTGCTTAACACCCTGTACAAGGCCA
  TTCTTGAATATGACAATGTTGGCGAAGCCTCTGAACAAACAGTCTCCCTCCTGTTCTCTCTCTGGGTGGA
  AACAGTGAGGCCCTACCTACAGACGGTAGATGAATGGATCGTCCATGGACACCTATGGGACGGCGCAAGG
  GAGTTTATCATACAGAGGAATAAAAACGTTCCAGTAAATCACAGAGACTTCTGGTATGCAACGTACACAC
  TCTATAGCGTATCTGAGAAGACAGAGAATGAAGACAAAGTGAGTGACAGTGCCAGTGCCAGCTCTGGCAG
  TGACCAGGGGCCCTCGAGCAGACAGCACACCATGGTGTCCTTCCTGAAGCCTGTCCTGAAGCAGATCATA
  ATGGCTGGCAAGTCAATGCAGCTGCTGAAGAACCTCAACTGTGCAGAGGGCCCTGCGTGCCAGGCAGCAG
  CCAGAGATGCAGAAAGAAAAAGCTTATATACCCTCTTTCTGGAATCTATACAGTTACGTCTTCAACATGG
  AGAAGATTCTGCTCCACACATTGTTAATGAAGATCAAACAACCAAAGAGAACCTTATAAAGATGCAGTCC
  ATTGCTGAAAGGCACCTTGAGCTAGATGATATTCATGACCCACTGTTGGCCATCAACTTTGCAAGGTTGT
  ATTTGGAGCAGAGCGACTTTCACGAGAAGTTTGCTGGTGGTGACATATGCGTGGATAGATCATCAGAGTC
  TGTGACCTGCCAGACTTTTGAATTAACACTGAGGTCTTGCCTCTATCCTCACATTGATAAGCAGTATCTA
  CATTGCTGTGGGAATCTCATGCAAACCTTAAAGAGAGATTTCAGGCTGGTGGAGTACTTGCAGGCCATGA
  GGAATTTTTTCTTGATGGAAGGTGGAGACACCATGTATGACTTCTATACTTCAATTTTTGATAAAATAAG
  AGAAAAGGAAACCTGGCAGAATGTGTCTTTTCTTAATGTGCAGCTCCAAGAAGCAGTAGGACAGCGCTAT
  CCTGAAGACAGTTTACGTTTATCTATATCTTTTGAAAATGTTGATACAACTAAGAAGAAACTACCCGTTC
  ATATCTTAGATGGTCTTACCCTAAGCTACAAGGTCCCATGGCCTGTGGACATTGTTATAAGTGTAGAATG
  TCAAAAAATTTATAATCAGGTGTTCCTTCTCCTATTGCAAATAAAATGGGCAAAATACAGCCTGGATGTT
  CTACTATTTGGTGAACTGGGTAATGCTGCTGAAAGATCCCAAGCAAAGGAAGACATTCCACGTGATCAGG
  ACACACCCTCCCAGTTTGGACCACCAAAAGAGTCATTAAGACAGCAGATTCATCGCATGTTTCTCTTAAG
  AGTGAAGCTCATGCACTTTGTGAATAGTTTACACAACTACATCATGACCAGGATTCTTCACAGTACAGGG
  CTGGAGTTTCAGCATCAAGTTGAGGAAGCCAAAGATTTAGATCAGTTGATTAAAATTCACTACAGGTATT
  TGTCAACCATCCATGATCGATGTCTGCTGAGAGAAAAGGTCAGCTTCGTGAAAGAAGCCATCATGAAAGT
  GTTGAACTTGGCGCTCATGTTTGCAGAAGGTTGGCAGGCAGGCCTAGGGGCTTGGCAAATGGAATCTATA
  GAGAAGATGGAATCTGACTTTAAAAACTGCCACATGTTTCTGGTAACCATCTTAAATAAAGCTGTGTGTA
  GAGGATCTTTTCCCCACTTGGAATCTCTTGCACTGTCGCTCATGGCTGGCATGGAGCAAAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146190
• Insert Size: 3075 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146190.2, NP_666302.2
• RefSeq Size: 3681 bp
• RefSeq ORF: 3075 bp
• Locus ID: 233276
• UniProt ID: Q8BKN5
• Cytogenetics: 7 B5
• Gene Summary: Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function]