Rnf123 (BC057082) Mouse Untagged Clone

CAT#: MC223108

Rnf123 (untagged) - Mouse ring finger protein 123 (cDNA clone MGC:67486 IMAGE:5704926), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf123
• Synonyms: BC003945; Kpc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCAAGGGGACTGGCATGTCGTTCTCCCGAAAGAGCTATAGGCTGACCTCAGATGCTGAGAAGT
  CCAGGGTCACAGGCATCGTGCAAGAGAAACTACTGAGCGACTATCTGTACCGCATCTTTTCCCCTCCTGA
  CCGTGGACCCGCCGCAGCCACCAGCAGGAAACCGCTAAACTTCCATAACCTGCCTGAGCACGTGGACCAG
  CTGCTACAGGTGGACAGTGAAGACAACGAGAGCCAGGGACAAGTTGAAGGTCGACTTGGCCCATCTACTG
  TGGTCCTAGACCACACAGGAGGCTTTGAGGGGCTTCTCCTTGTGGATGATGACCTCCTGGGGGTGATTGG
  ACACAGCAACTTTGGCACTATCCGTTCTACCACATGTGTGTACAAAGGGAAGTGGGTCTACGAGGTGCTC
  ATCTCCTCCCAGGGCCTCATGCAGATCGGCTGGTGCACCATCAACTGCCGCTTTAATCAGGAGGAAGGGG
  TTGGAGACACACATAACTCCTATGCCTATGACGGCAACCGAGTGCGCAAGTGGAATGTTACCACCACGAA
  TTATGGCAAGGCGTGGGCTGCGGGGGACATTGTCAGCTGCCTAATTGATCTGGATGATGGGACTCTGTCC
  TTCTGCCTGAATGGCGTGTCACTGGGCACTGCCTTCGAGAACCTTTCCAGGGGCCTAGGAATGGCGTACT
  TCCCAGCCATCAGCCTGTCATTCAAGGAGTCTGTGGCATTCAACTTTGGCAGCCGTCCTTTGCGCTACCC
  AGTTGCGGGCTTCCGGCCCCTGCAGGACCCTCCGTTTGCTGACCTGGTCCGGGCACAGAGGTTGCTGGGC
  TGCTTCCAGGCAGTGCTAAGTGTGGAGCTGGACCCTGTGGAAGGGCGGCTGGTGGAGACGGAGAGCTCTG
  AGTGGCAGCTGCAAGGGCAGCCCACTGTCCTCCTCACGCTGGCCCACATCTTCCATCACTTTGCACCACT
  GCTGCGCAAGGTATACCTGGTGGAGGCTGTGCTAATGAGCTTCCTGCTGGGCGTTGTGGAGAAGGGCACA
  CCAGAGCAGGCGCAGTCTGTGGTACACCAGATCTTGGACCTCTTGTGGCTCTTCATGGAGGACTATGAGG
  TACAGGATTGCCTGAAGCAGTTGATGATGTCACTTCTACGTCTCTACCGATTCTCGCCTATTGTCCCAGA
  CCTGGGTCTACAGATCCACTACCTGCGCCTCACTATGTCCATCCTGAGACACGAGAAGTCCCGCAAGTTC
  CTGCTTAGCAATGTCCTTTTTGACATGCTCCGGTCCGTGGTCTTCTTTTATATTAAGAGTCCCCTGCGTG
  TGGAGGAAGCTGGCCTGAAGGAACTCATTCCCACCACCTGGTGGCCCCATCGCTCCAGCAGGGAGAGCAG
  AGACGGTAAGGAAGCAAGGGAGGAGACCACCGAAGAGCGGCAGCGGAGGCGAGCCTATGAGCGTGGCTGC
  CAAAGACTCAAGAAACGCATTGAAGTGGTGGAAGAACTGCAGGTCCAGATCCTGAAGCTGCTGTTGGACA
  ATAAAGATGACAATGGGGGTGAAGCTTCTAGGTACATCTTTCTGACAAAATTCCGAAAGTTCCTGCAGGA
  GAATGCCAGCGGCCGGGGGAACACACCCGTGCTCTGCCCCCCTGAGTACATGGTCTGCTTCCTACACCGG
  CTGGTGTCTGCCTTGCGCTTCTATTGGGATGAATACAAAGCTTCCAACCCCCGTGCTTCCTTCAGTGAGG
  AGGCTTACATCCCGCCCCAGATCTTCTATAATGGCAAGGTGGACTACTTTGACCTTCAGCGCCTTGGGGG
  CCTCCTCTCACACCTTCGAAAGACCCTTAAAGATGACCTTGCTTCCAAAGCCAACATCGTGATCGACCCC
  CTGGAGCTCCAGGCAGCCACCATGGATGACCTGGATGAGGATGAAGAGCCTGCCCCCTCAGCGGCCCAGG
  TGTGGCAGGAGGGACAGCGTCCGATGCAAGCCCTGGCCATCGGAGGGGCACTGCCCCTGCCCCGGCCAGG
  CTGGCTCAGTTCTCCAACCCTGGGCAGAGCCAACCGCTTCCTCAGCACGGCAGCTGTGAGCCTCATGACC
  CCACGGCGGCTTCTGAGCACCATGGAGAAAGTCAAAGTTCGCTCACTGAATGTGGAACAGAGGACCCGTG
  AGGACATTGAGGGCAGCCACTGGAATGAGGGCCTGCTGTTGGGGAGGCCCCCTGAAGAGCCTGAGCAGCC
  GCTTACCGAGAACTCGCTGTTGGAAGTCCTGGATGGCACAGTCATGATGTATAACCTCAGCGTTCACCAG
  CAGCTGGGCAAGATGGTGGGTGTGTCTGATGATGTCAACGAGTATGCAATGGCCCTAAGAGACACAGAGG
  ACAAGCTCCGTCGGTGCCCTAAGAGGAGGAAGGATATCCTTGCAGAGTTGACCAAGAGCCAGAAGGTTTT
  CTCAGAAAAGCTGGACCACCTGAGCCGCAGGCTTGCCTGGGTCCACGCCACAGTCTACTCACAGGAGAAA
  ATGCTGGATATCTACTGGTTACTGCGTGTCTGCCTACGGACCATTGAGCATGGGGACCGCACGGGGTCTC
  TCTTTGCCTTCATGCCTGAGTTCTACCTAAGTGTGGCTATCAACAGCTACAGTGCCCTGAAGAACTATTT
  TGGCCCTGTGCACAGCATGGAGGAACTCCCAGGCTATGAAGAGACCCTGACACGCTTAGCTGCCATCCTC
  GCCAAACACTTTGCTGACCCTCGAATAGTAGGCACTGATATTCGAGACTCACTGATGCAGGCCCTGGCCA
  GCTATGTGTGCTACCCACACTCCCTGCGGGCTGTGGAACGGATTCCTGAGGAACAGCGCATCGCCATGGT
  GAGGAACCTTTTGGCACCCTATGAGCAACGGCCCTGGGCCCAGACCAACTGGATCCTGGTGCGGCTTTGG
  AGGGGCTGTGGGTTTGGGTACCGCTATACACGGCTGCCACATCTGCTGAAAACCAAGCCAGAGGATGCCA
  ATTTGCCCAGCCTCCAAAAGCCCTGCCCTTCGACCTTGCTACAGCAGCACATGGCGGACCTGCTGCGACA
  AGGGTCTGATGTGGCACCGAGCTTCCTCAACAGTGTCCTTAACCAGCTCAACTGGGCCTTCTCTGAGTTC
  ATCGGCATGATCCAGGAGATTCAACAGGCTGCTGAACGCCTGGAGCGGAACTTTGTGGACAGCCGACAGC
  TCAAGGTCTGTGCCACCTGCTTTGACCTGTCGGTCAGCTTGTTGCGCGTCTTGGAAATGACCATCACGCT
  GGTACCTGAAATATTCCTTGACTGGTCCCGCCCTACCTCTGAGATGCTGCTTCGGCGTCTGGCACAGCTG
  CTGAACCAGGTGCTGAACCGGGTGACAGCTGAGAGGAACCTGTTTGACCGTGTAGTTACCCTACGGCTAC
  CTGGGCTGGAGAGTGTGGACCACTACCCTATCCTGGTGGCAGTGACTGGCATCCTGGTACGCCTCCTGGT
  GCACGGCCCAACCTCAGAGACAGAGCAAGCCACCTCTGTGCTCCTGGCTGATCCCTGCTTCCAGCTTCGT
  TCCATATGCTATCTCCTGGGGCAGCCAGAGCCCCTAGCACCTGGCACTACCTTGCCTGCCCCTGACCGGA
  AACGCTTCTCTCTACAGAGTTATACAGATTATATCAGCGCTGAGGAGCTGGCCCAGGTGGAACAGATGCT
  GGCTCACCTGACCGCTGCATCTGCCCAGGCGGCCGCCGCCTCCCTGCCCACCAATGAAGAGGACCTCTGC
  CCAATCTGCTACGCCCACCCCATCTCTGCTGTGTTCCAGCCTTGTGGTCACAAATCCTGCAAAGCCTGCA
  TCAACCAGCACCTGATGAACAACAAGGACTGCTTCTTCTGCAAAGCCACCATTGTATCTGTAGAGGACTG
  GGACAAGGCAGCCAACACAAGCGCCATGTCCTCAGCTGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057082
• Insert Size: 3963 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057082, AAH57082
• RefSeq Size: 4745 bp
• RefSeq ORF: 3962 bp
• Locus ID: 84585
• Cytogenetics: 9 F1
• Gene Summary: Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.[UniProtKB/Swiss-Prot Function]