Pcx (NM_001162946) Mouse Untagged Clone

CAT#: MC223023

Pcx (untagged) - Mouse pyruvate carboxylase (Pcx), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcx
• Synonyms: Pc; Pcb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223023 representing NM_001162946
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTGAAGTTCCAAACAGTTCGAGGGGGCCTGAGGCTCCTGGGTGTCCGCCGATCCTCCTCGGCCC
  CTGTTGCCTCCCCAAATGTCCGGCGTCTGGAGTATAAGCCTATCAAGAAAGTAATGGTGGCCAACAGAGG
  TGAGATTGCCATCCGAGTGTTTCGTGCGTGCACAGAGCTGGGTATCCGCACAGTGGCTGTCTACTCGGAG
  CAGGACACAGGGCAGATGCACAGGCAGAAAGCTGATGAAGCCTACCTTATTGGCCGTGGCCTGGCACCTG
  TGCAGGCCTACCTGCACATTCCAGACATCATCAAGGTGGCCAAGGAAAATGGTGTAGATGCGGTGCATCC
  TGGCTATGGGTTCCTCTCAGAGCGAGCAGACTTTGCCCAGGCCTGCCAAGATGCTGGAGTCCGGTTCATT
  GGTCCAAGCCCAGAGGTGGTCCGCAAGATGGGAGACAAGGTGGAAGCCCGGGCCATTGCCATCGCTGCAG
  GCGTTCCAGTGGTCCCCGGCACGGACTCCCCCATCAGCTCCCTGCACGAGGCGCATGAGTTCTCCAACAC
  CTACGGCTTCCCTATTATCTTCAAGGCCGCCTACGGAGGTGGGGGCCGCGGCATGCGGGTCGTGCATAGC
  TATGAGGAGTTGGAAGAGAATTACACCCGGGCCTACTCCGAGGCCTTGGCAGCCTTTGGGAATGGAGCGT
  TGTTTGTGGAGAAGTTCATTGAGAAGCCAAGGCACATTGAGGTGCAGATCCTAGGGGACCAGTATGGGAA
  CATCCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGTCGGCACCAGAAGGTGGTAGAGATCGCCCCT
  GCTACCCACCTGGATCCCCAACTTCGCTCACGTCTCACCAGTGACTCTGTCAAACTTGCCAAGCAGGTAG
  GCTATGAGAACGCCGGCACTGTGGAGTTCCTGGTGGACAAGCACGGCAAGCACTACTTCATCGAGGTCAA
  TTCCCGCCTGCAGGTGGAGCACACGGTCACCGAGGAGATCACAGATGTGGACCTGGTCCATGCTCAGATC
  CACGTGTCCGAAGGCCGGAGCCTGCCTGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAATGGCTGTG
  CCATTCAGTGTCGGGTCACCACCGAGGACCCTGCACGCAGCTTCCAGCCAGACACCGGCCGCATTGAGGT
  TTTCCGGAGTGGTGAGGGCATGGGCATCCGCCTGGACAACGCCTCTGCATTCCAGGGCGCTGTCATATCG
  CCCCACTATGACTCTCTGCTCGTCAAGGTCATTGCACACGGCAAAGACCACCCCACAGCTGCCACCAAGA
  TGAGCAGAGCCCTGGCCGAGTTCCGTGTCCGAGGTGTAAAGACCAACATCCCCTTCCTGCAGAATGTTCT
  CAACAACCAGCAGTTCCTGGCAGGCACAGTGGACACCCAGTTCATCGATGAGAACCCTGAGCTGTTCCAG
  CTTCGGCCTGCACAGAACCGGGCCCAGAAGTTGCTACATTACCTCGGACATGTCATGGTGAATGGCCCTA
  CCACTCCAATCCCTGTCAATGTGAGCCCCAGTCCTGTGGATCCTGCTGTTCCTGTGGTGCCCATAGGCCC
  ACCTCCAGCTGGTTTCAGGGACATCCTTCTGCGAGAAGGGCCAGAGGGCTTTGCCCGAGCTGTGCGGAAT
  CACCAGGGGCTGCTGTTGATGGACACAACCTTCCGGGATGCCCACCAGTCACTACTGGCCACTAGAGTGC
  GCACACATGATCTCAAAAAGATTGCGCCCTATGTTGCCCACAACTTCAACAAGCTCTTCAGCATGGAGAA
  CTGGGGAGGCGCCACGTTCGACGTTGCCATGCGCTTCCTGTACGAGTGCCCCTGGCGGCGGCTCCAGGAG
  CTCCGGGAGCTTATCCCGAACATCCCGTTCCAGATGCTACTGAGGGGGGCCAATGCTGTGGGCTACACCA
  ACTACCCTGACAACGTGGTCTTCAAGTTCTGTGAGGTGGCCAAAGAGAATGGTATGGACGTCTTCCGAGT
  CTTTGACTCCCTCAACTACTTGCCAAACATGCTGCTGGGCATGGAAGCAGCAGGCAGTGCTGGGGGTGTG
  GTGGAGGCTGCCATCTCATACACGGGGGACGTGGCTGACCCTAGTCGCACTAAATACTCACTGGAGTACT
  ACATGGGCTTAGCTGAAGAACTGGTGCGAGCTGGCACTCACATCCTGTGCATTAAGGACATGGCGGGCCT
  GCTGAAGCCTGCCGCCTGCACCATGCTGGTCAGCTCCCTCCGGGACCGATTCCCCGACCTCCCACTGCAC
  ATCCATACCCATGATACATCAGGGGCAGGTGTGGCAGCCATGCTGGCCTGTGCACAAGCAGGGGCTGATG
  TTGTGGACGTGGCAGTAGACTCCATGTCTGGGATGACCTCACAGCCAAGCATGGGGGCCCTGGTGGCCTG
  TACCAAAGGGACTCCTTTGGACACAGAGGTACCCCTGGAGCGTGTGTTTGACTACAGTGAGTACTGGGAA
  GGGGCTCGGGGACTGTACGCAGCCTTCGATTGCACGGCTACCATGAAGTCTGGCAACTCCGACGTGTATG
  AGAATGAGATTCCAGGGGGCCAGTACACCAACCTGCACTTCCAGGCCCATAGCATGGGGCTTGGCTCCAA
  GTTCAAGGAGGTCAAGAAGGCCTATGTGGAGGCTAACCAGATGCTGGGGGACCTCATCAAGGTGACACCA
  TCCTCCAAGATTGTGGGGGACCTGGCCCAGTTCATGGTGCAGAATGGGTTGAGCCGGGCAGAGGCAGAAG
  CTCAGGCAGAAGAGCTGTCCTTCCCCCGCTCTGTGGTGGAGTTCCTGCAGGGCTACATTGGCATTCCCCA
  TGGGGGTTTCCCTGAGCCCTTTCGCTCTAAGGTGCTAAAGGACCTGCCAAGAATAGAGGGGCGGCCTGGA
  GCCTCCCTCCCTCCCCTGAACCTGAAGGAGCTGGAGAAGGACCTGATTGATAGGCATGGGGAGGAGGTGA
  CCCCAGAGGACGTCCTCTCTGCAGCCATGTACCCTGATGTCTTTGCTCAATTCAAAGACTTCACGGCTAC
  CTTCGGCCCCCTGGATAGCCTTAATACTCGTCTCTTTCTTCAAGGACCCAAAATTGCAGAGGAGTTTGAG
  GTTGAGCTGGAACGGGGCAAGACCCTGCACATCAAAGCCCTGGCTGTAAGCGACCTGAACCGTGCTGGCC
  AGAGGCAGGTGTTCTTTGAACTCAATGGGCAGCTTCGATCCATTCTGGTTAAAGACACCCAGGCCATGAA
  GGAGATGCACTTCCATCCCAAGGCTTTGAAGGATGTGAAGGGCCAAATTGGGGCCCCGATGCCTGGGAAG
  GTCATAGACATCAAGGTGGCAGCAGGGGACAAGGTGGCTAAGGGCCAGCCCCTCTGTGTGCTCAGCGCCA
  TGAAGATGGAGACTGTGGTGACTTCGCCCATGGAGGGCACTATCCGAAAGGTTCATGTTACCAAGGACAT
  GACTCTGGAAGGCGACGACCTCATCCTAGAGATTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001162946
• Insert Size: 3540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162946.1, NP_001156418.1
• RefSeq Size: 4148 bp
• RefSeq ORF: 3540 bp
• Locus ID: 18563
• Cytogenetics: 19 4.07 cM