Wdr59 (NM_176923) Mouse Untagged Clone

CAT#: MC223017

Wdr59 (untagged) - Mouse WD repeat domain 59 (Wdr59), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wdr59
• Synonyms: 5430401O09Rik; Cdw12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223017 representing NM_176923
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCGCTGGAGCAGCGAGAACGTGGTGGTGGAGTTTCGAGACTCTCAGGCAACTGCCATGTCTG
  TGGACTGTCTTGGGCAGCATGCAGTGCTTTCTGGCCGACGATTCCTGTACATCGTCAATCTAGATGCCCC
  TTTTGAAGGTCACCGGAAGATCTCCCGCCAGAGCAAATGGGACATTGGAGCTGTGCAGTGGAACCCTCAT
  GACAGCTTCGCACACTATTTTGCAGCTTCGAGTAATCAACGGGTCGACCTTTATAAGTGGAAAGATGGCA
  GCGGGGAAGTTGGTACAACTTTACAGGGGCACACTCGAGTTATCAGCGACTTGGACTGGGCAGTGTTTGA
  GCCAGATCTCCTGGTCACCAGCTCTGTGGACACCTACATCTACATCTGGGATATCAAAGACACAAGGAAA
  CCTACTGTTGCACTGTCGGCTGTAGCTGGTGCCTCCCAGGTCAAATGGAATAAGAAGAACGCCAACTACC
  TTGCCACCAGTCACGATGGTGACGTACGGATATGGGACAAGCGAAAACCCAGTACAGCTGTGGAATATCT
  AGCAGCCCACCTCTCCAAGATCCACGGCCTGGACTGGCACCCAGACAGTGAGCACATCTTTGCCACCTCC
  AGTCAAGATAACTCTGTCAAGTTCTGGGATTACCGCCAGCCTCGGAAATACCTCAATATCCTCCCTTGCC
  AGGTGCCTGTCTGGAAAGCCAGATACACGCCTTTCAGCAACGGGCTAGTGACCGTGATGGTTCCCCAGCT
  GCGGAGGGAGAACAGCCTGCTCCTGTGGAACGCGTCTGACTTGAACGCTCCAGTTCACACCTTTGTGGGT
  CATGATGACGTGGTGCTGGAGTTCCAGTGGAGGAGGCAGAAGGAAGGATCCAAGGACTATCAACTGGTTA
  CATGGTCCCGTGATCAGACCTTGAGAATGTGGCGAGTGGACTATCAAATGCAGAGGCTCTGTGCCAATGA
  CATACTGGATGGTGTTGATGAGTTCATTGAGAGCATCTCTCTCCTGCCGGAACCAGAGAAGACCCCACAT
  CCTCAAGACATAGATCACCAGCCCAGCTTAAGCCATGGAGAGGAAGATGCCATAAAGGAAGACCCTCCCA
  GCAGTTTGCTGGAAGAGAAGAGATCAGACCAACTAGGCCTCCCCCAGACTCTGCAACAGGAGTTCTCCTT
  GATCAACGTGCAGATCCGGAATGTCAATGTGGAGATGGACGCTGCAGACCGGAGCTGCACAGTGTCTGTG
  CACTGCAGCAACCACCGTGTGAAGATGCTGGTGACCTTCCCGGCGCAGTACCCTAACAACGCTGCTCCTT
  CCTTCCAGTTCATTAATCCTACCACTATCACGTCTGCCGTGAAGGCCAAGCTGCTGAAGATCCTAAAGGA
  CACTTCACTGCAGAAAGTGAAACGCAACCAGAGCTGTTTGGAGCCTTGCCTGCGCCAGCTTGTCTCGTGC
  CTTGAGTCTTTTGTGAACCAAGAGGACAGTGCTTCTAGCAACCCATTTGCACTCCAAAACTCTGTCACAC
  CACCCTTGCCGACATTTGCACGGGTGACCACTGCCTACGGCTCCTACCAGGATGCCAACATTCCCTTTCC
  TAGGACCTCTGGGGCCAGGTTCTGTGGAGCAGGTTACCTGGTGTACTTCACACGACCCATGACGATGCAT
  CGAGCAGTGTCTCCCACAGAGCCTACTCCTAGGTCTCTCTCAGCCCTGTCTGCTTATCATACTGGTTTGA
  TTGCACCAATGAAGATCCGCACCGAGGCCCCTGGGAACCTGCGTCTGTACAGTGGGAGCCCAACTCGCAG
  TGAGAAAGAGCAGGTCTCCATCAGTTCCTTCTATTACAAGGAACGGAAATCGAGGAGGTGGAAGAGTAAG
  CGGGAAGGCTCTGACTCCGGCAACCGACCCATTAAGGCTGCTGGCAAGGTCATCATCCAGGATGTCTCGT
  GCCTCCTTCCCGTTCATAAGTCCTTGGGAGAGCTGTACATATTGAATGTGAACGACACTCAGGAGACATG
  TCAGAAGAATGCCACTTCTGCCATGCTTGTGGGAAGGAAGGATCTTGTCCAGGTTTGGTCGCTGGCTACG
  GTAGCTACAGATCTTTGCCTTGGGCCGAAGTCTGACCCAGATTTGGAAACACCTTGGGCTCGACATCCAT
  TTGGGCGACAACTGCTGGAGTCCCTCTGGGGAGACCGAGAGAGCACAAGAGTGTGTGGACCACCTCTCTC
  AGGTGCTAGACTGGCTCACTATTGCCAACTCCGAGATGTTCAGACCTTGGCTATGCTCTGTAGCGTGTTT
  GAAGCCCAGTCCCGGCCTCAGGGCTTACCAAACCCTTTTGGGCCTTTCCCTAATCGTTCTTCTAATCTTG
  TGGTGTCCCACAGCCGCTATCCCAGCTTCACCTCCTCGGGGTCCTGCTCCAGCATGTCAGATCCAGGGTT
  CAACACCGGTGGCTGGAACATAGGAAGAGAGACAGAACACATATCTTCACCTTGGGGGGAGTCTTCGCCA
  GAGGAGCTCCGCTTTGGGAGTCTGACCTACAGTGACCCCCGGGAGCGGGAACGAGACCAGCATGATAAAA
  ACAAAAGGCTCCTGGACCCTGCCAATACCCAGCAGTTTGACGACTTTAAGAAGTGCTACGGAGAGATCCT
  CTACCGCTGGGGTCTGAGAGAGAAGCGAGCAGAAGTGCTGAAGTTTGTGTCCTGCCCTCCTGACCCTCAC
  AAAGGGATCGAGTTTGGTGTGTACTGCAGTCACTGTCGGAGCGAGGTCCGTGGCACCCAGTGCGCCATCT
  GCAAAGGCTTCACCTTCCAGTGTGCCATCTGCCATGTGGCCGTGCGGGGTTCATCCAACTTCTGCCTGAC
  CTGTGGGCATGGTGGCCACACGAGCCACATGATGGAGTGGTTTCGGACCCAGGAGGTATGTCCCACGGGG
  TGTGGCTGCCACTGCCTGCTGGAGAGCACCTTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_176923
• Insert Size: 2976 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176923.4, NP_795897.2
• RefSeq Size: 5162 bp
• RefSeq ORF: 2976 bp
• Locus ID: 319481
• UniProt ID: Q8C0M0
• Cytogenetics: 8 E1
• Gene Summary: As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.