Recql5 (NM_130454) Mouse Untagged Clone

CAT#: MC222962

Recql5 (untagged) - Mouse RecQ protein-like 5 (Recql5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Recql5
• Synonyms: RecQ5; Recq5b; Recql5b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222962 representing NM_130454
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCCGTCCTTTCTCCACTCCCTTCGACCGGGAGCGGCGAGTCCGGAGCACCCTGAAGAAAGTCT
  TTGGGTTTGATTCTTTTAAAACACCTTTACAAGAGAGTGCGACCATGGCTGTAGTGAAAGGTGCCGAGGA
  TGTGTTTGTGTGCATGCCCACAGGGGCAGGGAAGTCTCTGTGCTATCAACTCCCTGCCCTGTTGGCCAGT
  GGGATCACCATTGTAGTCTCTCCTCTCATTGCCTTGATTCAGGACCAGGTAGACCATTTGCTGGCCTTGA
  AGGTACAAGTTAGTTCCCTGAACTCAAAGCTCTCGGTACAGGAGAGGAAGGAGCTGCTGTCTGACCTGGA
  GCGAGACAAACCTCGGACCAAACTTTTGTACATCACCCCAGAGATGGCAGCTTCAGCCTCCTTCCAGCCC
  ACCCTGAATTCCCTGGTGTCCCGAAACCTGCTCTCCTACTTGGTGGTGGATGAAGCTCATTGCGTTTCCC
  AGTGGGGACATGACTTCCGTCCTGACTATTTGCGTCTGGGTGCCCTACGTTCCCGCTTGGCACATGCCCC
  GTGCGTGGCTCTGACGGCTACAGCCACCCCACAGGTTCAAGAGGATGTGTTTGCTGCCCTGCACTTGAAG
  CAGCCAGTCGCCTCTTTCAAGACTCCCTGTTTCCGGGCCAACCTCTTCTATGATGTGCAGTTCAAGGAAC
  TGATTCCCGACGTCTATGGCAATCTGAGGGACTTCTGCCTTAAGGCTCTTGGACAGAAGGCTGAGAACGG
  GTCGTCCTCTGGCTGTGGCATTGTCTACTGCAGGACTAGAGAAGCTTGTGAACAACTGGCCATTGAGCTC
  AGCAGCAGGGGTGTGAACGCCAAGGCTTACCACGCAGGCCTGAAGGCATCTGATAGAACACAGGTACAGA
  ACGAGTGGATGGAGGAGAAGGTCCCTGTAATTGTTGCGACCATCAGTTTTGGGATGGGAGTAGACAAAGC
  TAATGTCCGGTTTGTTGCTCATTGGAATATTGCCAAGTCCATGGCTGGCTACTACCAGGAGTCTGGCCGT
  GCTGGCAGGGATGGCAAACCATCTTGGTGCCGCCTGTATTACTCTAGGAATGACCGAGACCAAGTCAGCT
  TCCTTATCAGGAAGGAACTAGCCAAACTCCAGGAAAAGAGGGGGAACAAACCGTCTGATAAGGCCACCCT
  GCTGGCCTTTGATGCCCTGGTGACCTTCTGTGAAGAAGTGGGGTGCCGCCATGCTGCCATTGCTAAGTAC
  TTTGGGGATGCACCCCCCGCCTGCGCCAAAGGCTGTGACTACTGCCAGAATCCTGCAGCCATAACGAAGA
  AGCTGGATGCCCTGGAGCGCAGCAGCAGCTGGAGCAAGACCTGCATCGGGCCCTCACAGGGGAATGGCTT
  CGATCCTGAGCTGTATGAGGGAGGCCGCAGGGGCTATGGTGGCTTCAGCAGGTATGATGAAGGTTCTGGA
  GGTAGCGGTGATGAGGGCAGAGATGAGGCCCACAAGCGGGAATGGAATCTTTTCTATCAGAAACAGATGA
  GCCTGCGCAAGGGCAAAGAGCCCAAGATAGAAGAATTCACGCCCCCAGATGAAGACTGTCCCCTGAGAGA
  GGCTTCAAGCAGGAAGATCCCGAAGCTCACAGTGAAGGCCCGTGAGCACTGCCTGCGACTTCTGGAGGAG
  GCTCTGATCAGTAACCATCAGGCTGCAGGCTCCACTCATGGAGCTGACCTGCAGGCCAAGGCTGTGGAAC
  TGGAGCATGAGACATTCCGGAATGCCAAGATGGTCAATCTGTACAAGGCCAGTGTCCTGAAGAAGGTGGC
  CGAGATCCACAAAGCCTCCAAGGATGGTCAGCTCTATGACATGGAAAGCGGCACCAAGAGCTGTGGGGCC
  GCAGCTGAGTTCTCGGAGCCCAGTGACTATGACATCCCGCCAACCTCACATGTGTACTCGCTCAAACCCA
  AGAGAGTAGGAGCTGGCTTCTCCAAAGGCCCCTGCTCATTCCAGACAGCCACAGAACTGTTGGGGAAGTC
  TCACTCCCAGAAGCAAGCCCCAGAGGCTATGCTAGAGGGTGGACAGGAGCCCCCAGGCTGGGTCTGTGAC
  CTTCAAGATGAAGACAGGAGCAAGCCCCACCCTGGGTACCAAGAAAAGGCTCTAGGAAGCAGTGTTAATT
  GTGGGGACCCCTCGCCTGAGAAGAAGACGAAAGGGTCTTCCCAGGGCAGTGCCAAAGCTAGAGCCAGCAA
  GAAACAACAGCTCCTAGCCACAGCAGCCCGCAAGGATTCCCAGAATATCACTCGATTCCTCTGTCAGAGG
  ACAGAATCTCCACCTCTCCCTGCTTCAGTTCCAAGATCAGAAGATGCCAGCCCCTCCTGTGGGGATGTCC
  CAGGGAAGTGCACACAAGAGGTTGGAGCTCAGGGACATTTGGTTGCTGTATTTCAGACTGAGGGTCCCAG
  GGAGAGGCCAAGCACCTGCTCACTCAGAGACCAGAGCTTCCCTGAAGGTCAGCCTAGCCCTCTAAAGGAA
  ACCCAGGCAGAGAAGCGGCCCAGACCACAGCAGGGCAACCCAGAGAGAAGGGCTCAAAAGAGGCTTCGCC
  CCTCAACCAAGTCCTCCATCTTGGCTGAGGCCAAGGACAGTACCTTGGCCAGTGATCGCAGCACTGAAAA
  CAAAGTGGCTCAAGAACCCTGCCAGCTCTCAGCATCTGGCACCTCCCTGAGGGAGGCCGCAGACATTGTG
  GTCAGGCACCTGACCCCCTTCTACAAGGAGGGCAGGTTTATATCCAAGGACCTGTTTAAGGGCTTTGCCC
  GCCACCTCTCACATTTGCTGGCTCAGCAGCTCTCTCCTGGAAGGAGTGTGAAGGAAGAGGCCCAGAGCCT
  CATCAAGCAGTTCTTCCACAATCGTGCCCGCTGTGAGAGTGAAGCTGACTGGCACAGCCTGCGTGGCCCA
  CAGCGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_130454
• Insert Size: 2949 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130454.2, NP_569721.1
• RefSeq Size: 4001 bp
• RefSeq ORF: 2949 bp
• Locus ID: 170472
• UniProt ID: Q8VID5
• Cytogenetics: 11 E2
• Gene Summary: DNA helicase that plays an important role in DNA replication, transcription and repair. Inhibits elongation of stalled transcripts at DNA damage sites by binding to the RNA polymerase II subunit POLR2A and blocking the TCEA1 binding site. Required for mitotic chromosome separation after cross-over events and cell cycle progress. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination (By similarity).[UniProtKB/Swiss-Prot Function]