Pacs1 (NM_153129) Mouse Untagged Clone

CAT#: MC222844

Pacs1 (untagged) - Mouse phosphofurin acidic cluster sorting protein 1 (Pacs1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pacs1
• Synonyms: AI325977
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222844 representing NM_153129
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACGCGGAGGGGCGGGCGGTGGTCCCGGAGGCTCCGGGGGCGGCAGCAGTCAGCGGGGCTCTG
  GGGTCGCCCAGTCCCCGCAGCAGCAGCCGCAGCAGCAGCCGCCGCAGCCTCAACAGCCGACGCCCCCCAA
  GCTGGCCCAGGCCACCTCGTCCTCCTCGTCCACCTCGGCTGCGGCCGCCTCCTCCTCGTCCTCCTCCACC
  TCCACCTCCATGGCCGTCGCGGTGGCCTCGGGCTCTGCGCCTCCGGGCGGTCCAGGACCAGGCCGCACCC
  CCGCCCCAGTGCAGATGAATTTGTATGCTACCTGGGAGGTGGACCGGAGCTCATCCAGCTGCGTGCCCAG
  GCTCTTCAGCTTAACTCTGAAGAAACTTGTTATGCTTAAAGAAATGGACAAAGATCTTAACTCAGTAGTC
  ATTGCTGTGAAGCTGCAGGGTTCAAAAAGGATTCTTCGCTCCAATGAGATCATCCTTCCAGCCAGTGGCC
  TGGTGGAGACAGAGCTCCAGTTAACCTTCTCCCTCCAGTACCCTCATTTCCTTAAGCGAGATGCCAACAA
  ACTGCAGATTATGTTGCAAAGGAGGAAGCGGTACAAGAACCGAACTATCTTGGGCTATAAAACCTTAGCT
  GTGGGACTCATCAACATGGCAGAGGTGATGCAACACCCTAATGAGGGCGCTTTAGTACTTGGCCTGCACA
  GCAATGTGAAGGATGTCTCTGTGCCAGTGGCAGAAATAAAAATCTACTCTTTATCCAGCCAGCCCATTGA
  CCATGAAGGAATCAAGTCTAAGCTGTCTGATCGCTCCCCAGATATTGACAACTACTCTGAAGAAGAGGAG
  GAAAGCTTCTCCTCAGAGCAGGAAGGCAGTGACGACCCATTGCATGGGCAGGACCTATTCTATGAAGATG
  AAGATCTTCGGAAAGTAAAGAAGACTCGGAGGAAACTGACCTCAACCTCAGCCATCACTAGGCAACCTAA
  CATCAAACAGAAGTTTGTGGCCCTCCTGAAACGATTCAAAGTTTCTGACGAGGTGGGCTTTGGGCTAGAA
  CACGTGTCCCGGGAGCAGATTCGGGAAGTGGAGGAGGACTTGGATGAACTGTACGACAGTCTAGAGATGT
  ACAACCCCAGCGACAGTGGCCCTGAGATGGAGGAAACAGAAAGCATCCTCAGCACCCCAAAGCCCAAGCT
  CAAGCCCTTCTTTGAGGGGATGTCTCAGTCCAGCTCACAGACGGAGATTGGCAGTCTCAACAGCAAGGGC
  AGCCTCGGGAAAGACACCACCAGTCCTATGGAGTTGGCTGCTTTAGAAAAAGTCAAATCTACCTGGATTA
  AAAACCAAGATGACAGCTTGACTGAAACAGACACTTTAGAAATCACTGACCAGGACATGTTTGGAGATGT
  GAGCACAAGTCTGGTTGTACCAGAAAAAGTCAAAACTCCTATGAAGTCCAGCAAAGCTGATCTGCAGGGC
  TCTGCCTCTCCCAGCAAGGTGGAAGGGACACACACACCTCGGCAGAAGAGGAGCACTCCCCTGAAGGAGC
  GACAGCTCTCCAAGCCCCTGAGTGAGAGAACCAACAGCTCTGACAGTGAGCGATCTCCTGATCTGGGCCA
  CAGCACACAGATTCCAAGGAAGGTGGTATACGACCAACTGAATCAGATCCTGGTATCGGATGCAGCCCTC
  CCTGAAAATGTCATCCTGGTGAACACCACAGACTGGCAGGGCCAGTACGTGGCTGAATTGCTTCAAGACC
  AGAGGAAGCCTGTGGTATGCACCTGCTCAACAGTGGAGGTCCAGGCAGTGCTGTCCGCCCTTCTCACTAG
  GATTCAGCGCTACTGCAACTGCAACTCATCCATGCCGAGGCCGGTCAAGGTGGCAGCTGTGGGCAGCCAG
  AGCTACCTGAGCTCCATCCTCCGTTTTTTTGTCAAGTCCCTTGCCAGCAAGACCCCAGACTGGCTGGGTT
  ACATGCGCTTCCTCATCATTCCCCTAGGTTCTCATCCAGTGGCCAAATACTTGGGCTCAGTAGATAGTAG
  ATATAGCAGCACCTTTCTTGATTCTGCCTGGAGAGACCTATTCAGTCGCTCAGAGCCTCCAGTGTCAGAG
  CCACTGGACGTGGTGGGTCGTGTGATGCAGTATGTCAATGGAGCTACCACAACACACCAGCTGCCTGTGG
  CTGAAGCCATGTTGACCTGCAGGCATAAGTTCCCTGATGAGGACTCCTACCAGAAGTTCATCCCCTTCAT
  TGGCGTTGTGAAAGTGGGCCTTGTTGAAGACTCGCCCTCCACTGCAGGTGATGGGGACGATTCACCTGTG
  GTCAGCCTTACTGTGCCCTCCACATCACCACCCTCAAGCTCAGGCCTGAGCCGAGATGCCACAGCTACCC
  CTCCCTCGTCCCCATCCATGAGCAGTGCCCTCGCCATCGTGGGGAGCCCAAATAGCCCATATGGGGATGT
  GATTGGTCTCCAGGTGGACTACTGGCTGGGCCACCCTGGGGAGCGAAGGAGAGAAGGTGATAAGAGGGAT
  GCCAGCTCAAAGAACACCCTCAAGAGTGTCTTCCGCTCGGTGCAGGTTTCTCGCCTGCCACATGCAGGGG
  AAGCCCAGCTTTCTGGCACCATGGCAATGACTGTGGTCACCAAAGAGAAGAACAAGAAAGTGCCCACTAT
  CTTCCTGAGCAAGAAGCCCCGAGAAAAGGAGGTGGATTCTAAGAGCCAGGTCATCGAGGGCATCAGCCGC
  CTCATCTGTTCTGCAAAGCAGCAGCAAACCATGCTGAGAGTGTCCATCGATGGAGTTGAGTGGAGTGACA
  TCAAGTTCTTCCAGCTGGCAGCCCAGTGGCCAACTCATGTCAAGCACTTTCCAGTGGGACTCTTCAGTGG
  CAGCAAGACCACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153129
• Insert Size: 2886 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153129.2, NP_694769.2
• RefSeq Size: 4361 bp
• RefSeq ORF: 2886 bp
• Locus ID: 107975
• UniProt ID: Q8K212
• Cytogenetics: 19 A
• Gene Summary: Coat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin-coated membrane pits (By similarity).[UniProtKB/Swiss-Prot Function]