Glis3 (NM_175459) Mouse Untagged Clone

CAT#: MC222716

Glis3 (untagged) - Mouse GLIS family zinc finger 3 (Glis3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Glis3
• Synonyms: 4833409N03Rik; E330013K21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222716 representing NM_175459
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGAAGGTCATGTGGCATGAATCTCCACCGGACATCAAGAACCCCACAGGGGCCTGGCCTACTCG
  GTGGTCAACATATCCCTCCCATCAGAGCCCACGCAGGCACTCCCTGCTCCTCTTCCTGTGCCAGCACGCC
  GAGCCCCAGTATTGGAAGTCTTGCTAACAGTCTTCACCTCAAGATGTCCTCGGGAGCAGGGATGGCTCCT
  CAGAGCAACATGGCCGCGAGTCCCATCCATCTGCCTGCCCTGAGCCCCAGGAGACAGTTACTCGCCAATG
  GGAAGCCTCAGTTCCAGGTCACCCCGGCTGGTGTCATGGCAGCACCACATACTATAAAGCCCAAACAGCA
  AGAATTTGGAGACCCCTTCTCTCCAAATCCTGAGAAAGGGGCTCTTGGCTTTGGGCCTCAGTGCAAGTCC
  ATTGGAAAAGGCAGCTGCAACAATCTAGTGGTCACCAGCAGTCCCATGATGGTTCAGCGACTGGGACCCA
  TTTCACCTCCAGCAAGCCAGGTCTCCACAGCATGCAAGCAGATCAGTCCTAGCTTACCGAGGGCAGTGAA
  TGCAGCCAACCTGAATAGACCTCCTTCAGATACCAGGTCCGTTATTTTGCAAGAGTCTCTGGTGTCCACG
  ACTTTGAGTCTGACAGAGAGTCAATCAGCCTTGAGTGTGAAGCAAGAGTGGTCTCAGAGCTACAGGGCTT
  TCCCTTCACTTTCATCCAGCCACAGTTCCCAGAATGGCACGGACCTAGGGGACCTTCTTAGCTTGCCTCC
  AGGCACGCCAGTGTCTGGCAACAGCGTCTCCAACTCGTTGCCACCCTACCTTTTCGGCATGGAAAATAGC
  CACTCTCCTTACCCTAGCCCTCGGCACTCAGCAACCAGGGCCCACTCCACCCGCTCTAAGAAGAGAGCAT
  TGTCCTTGTCGCCACTGTCAGATGGCATCGGGATCGACTTCAACACTATCATCCGTACTTCACCCACATC
  CTTGGTCGCCTACATCAACGGACCAAGAGCCTCCCCAGCCAACCTGTCCCCACAGTCAGAGGTCTATGGG
  CATTTCCTGGGTGTTCGTGGCAGCTGCATCCCCCAGTCTTGTGCAGTGGCCAGCGGGCAGAAAGGCATAT
  TGGTTGCCAGTGGAGGGCATACGCTGCCGGGCTATGGAGAGGACGGTACACTGGAGTATGAACGCATGCA
  GCAGCTTGAGCATGGTGGCCTGCAACCCGGACCTGTAAACAACATGGTGTTGCAGCCTGGCCTACCGGGC
  CAGGATGGCCAGACAGCCAACATGCTCAAAACAGAGCGCCTGGAGGAGTTCCCCGCCAGTGCCTTGGACC
  TGCCCTCTGCTCTGCCTCTCCCTCTTCCTCCGCCTCAGGGTCCCCCACCCCCATACCATGCCCATCCACA
  CCTTCATCACCCAGAGCTCCTGCCTCACACCCAATCGCTGTCCCTGGCCCAGACTGGCCTGGAAGAGGAT
  GGGGAGATGGAAGACTCAGGGGGGAAGCACTGCTGCCGTTGGATAGACTGCAGTGCCCTTTATGACCAGC
  AGGAGGAACTGGTGAGGCACATCGAGAAGGTCCACATAGACCAACGCAAGGGGGAAGACTTCACGTGCTT
  CTGGACTGGCTGCCCTAGAAGATACAAGCCTTTCAACGCACGGTATAAACTGCTGATCCACATGAGGGTC
  CACTCTGGGGAGAAGCCCAACAAGTGTACGTTCGAAGGCTGCAAGAAGGCCTTTTCCAGGCTCGAGAACC
  TCAAGATTCACTTGCGGAGCCACACGGGTGAGAAGCCATACTTGTGCCAGCATCCGGGCTGCCAAAAGGC
  CTTCAGCAATTCCAGTGACCGTGCCAAGCACCAGAGGACACATCTTGACACTAAACCTTATGCTTGTCAA
  ATTCCAGGATGTACAAAGCGCTACACAGACCCAAGCTCCCTCAGAAAGCACGTGAAGGCACATTCTTCCC
  GAGAGCAGCAAGCAAGGAAAAAGCTACGGTCTAGCACTGAGCTCCATCCAGATCTCCTCACAGACTGCCT
  CGCTGTGCAGCCCCTGCAGCCAGCCACGTCCCCTGGAGATGCTGCTGATCATACCGTGGGGCACTCCCCA
  GGGCCGGGGCCTGGGCCAGGGCCTGGGGCTGAACTCTATTCAGCTCCCATTTTCGCCAGCAATCATTCAA
  CTCGAAGTGGAACAGCTGCTGGGGCTGGGCCACCCCCACATCCTGTCAGTCACCCTTCTCCAGGACATAA
  TGTACAGGGGAGCCCCCACAACCCCTCCTCCCAGTTACCTCCACTCACAGCTGTGGACGCAGGCGCTGAG
  AGGTTTGCACCTCCGACTCCATCTCCTCACCACATCAGCCCGGGAAGAGTTCCAGCTCCTCCGTCACTGC
  TCCAGAGAGCACAGGCTCCCCACTCCCAGCAGCCACCAGGCTCACTCCTGAAGCCCTACCAGCCAGAAAC
  CAACTCTTCTTTTCAGCCAAATGGTATCCATGTCCACGGATTTTATGGCCAGCTGCAGACATTCTGTCCC
  CCGCATTATCCTGATTCCCAAAGAACTGTGCCACCCAGCGGCTCCTGCAGTATGGTGCCTTCCTTTGAGG
  ACTGCCTGGTCCCCACGTCCATGGGTCAAGCCGGTTTTGATGTTTTCCACAGAGCCTTCTCAACACACTC
  AGGCATCACAGTGTACGATTTGCCTTCAGCTTCCTCAAGCCTCTTTGGGGAATCTCTTCGCAGTGGCCCT
  GAAGACCCCACGTTCTTGCAGCTCAGTGCTGTGGACCGCTGTCCTAGCCAGCTCTCCTCTGTGTATACCG
  AAGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175459
• Insert Size: 2808 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175459.6, NP_780668.3
• RefSeq Size: 7524 bp
• RefSeq ORF: 2808 bp
• Locus ID: 226075
• Cytogenetics: 19 C1
• Gene Summary: This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein which contains multiple C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the transcriptional regulation of insulin. It is thought to enhance GLI-RE-dependent transcription by binding to the GLI-RE consensus sequence (GACCACCCAC). Mutations in a similar gene in human have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).