Spidr (NM_146068) Mouse Untagged Clone

CAT#: MC222714

Spidr (untagged) - Mouse RIKEN cDNA 2310008H04 gene (2310008H04Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spidr
• Synonyms: 2310008H04Rik; Kiaa0146
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222714 representing NM_146068
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGAGCCCGCCGCCCCGGCACGTCCAAGAGGAAAAGGAATTGGCATATAGAACACCCATCCTTTC
  GAGAAGAGAGGTCACAGCAGTTGAGACGGGGAAATTTCAAGACAGTGGAAGCAGCTGACTCTCTCTCTAA
  AGCATGGCTCAAGTGTGGAGAAGGATTTCAGGACACCTCTGAGATCCTGTCATTAGCAAGTGAAAAGACA
  GGTATTACTGAAAAGCACCTGGAATTATCTCCAAAACCAAAGACAGAAACTACAAGTAAGAATGCTAGTG
  AGCTTCCAAATATAATATGGAGTTCCAGTGAAAGTGATTTCTCCGATGAAGATAAGACACTTCCTGCATT
  ACAGAGAGATGGTAGGCATGGCCCTAGAGCCGACAGGCTTGGTGACAGGACTATCTCATGTCCCGAAGAC
  GAAGACATTGAAGATGAATTACAGGTTATTGACTGGGAGGTTAACAGTGACAAAGAAGATCCTGGTGGGC
  CTAGTGAGTGTGAAGATGATAAGGGTACCCTGGACATCTCAGATTGTGATTCCTGTGCAAGTCTGACAAG
  TGATGACAGGCTATGTGAGCCTTCTGAGCCAATTTCTACAGAAATTTTGGAGTATTCATCAGATAGTGAA
  AAAGAAGAAGACCCAGAACATTCCCTGTTCATTGATTCAGAATCCCCTCACAAATACCAGGCGGATTTTA
  AATCTGATGCAAGATGGTGTTTGGTTAGTCAGACTGACTCAGAAGCAAATTCAGCTGAGCCTACATTGAC
  ACCCCAGAAATATACGGTCAAGTTTCCGAAGACTCCAGAATATTCAGTAACAAAGAAGAAGCTTTTAAGG
  GGCGGGCTAGCAGAAAGACTCCAAGAACTGCAGAATCGAAAGAGATCTGCCATTTCTTTGTGGAGACATC
  GATGTGTTTCTTACCAAATGACACCATTAGGCAGAAAATCTGGTGTATTAACTGTGAAAATTCTAGAACT
  GCATGAGGAATGCAGCATGCAAGTTGCAGTATGTGAGCAGTTAGCAGGGCCTCCAATCACCAGCCCTCCC
  GGAGGACTAGCTCCTAGACCAGGAGCCTACCTGAAGGTACTCTTCACCAGAGAGACTGCAGACCACTTGA
  TGGGGCATCCCCAGGACATCATTTACATCTTCCCTCCTTGGCAAAAACTTCTTATCCCAAATGGAAGTTG
  CTCCATTATTCTGAATACTTATTTTTGTCAGAAAGCTATTGCAAAAGAAACAGTGCGCGAAGACCTATAC
  AGTCCGGACATATCTCTTTCAAGGAGAAATATAACTTTGGCCCAGACATTTAGAATTAAGGACATAACAG
  ACAATTCATCCATCAATCAGACTACATATGATAGCCTGGCCACCCCTGGCACAGGCTGGACCCACGGACA
  TGAGAAGGCAGAGCAACATCTTATAGTTGCTGCTCCCCTGAGGAATTCTCTTCTGGACATTGTGGAAAGC
  CAAAGAGCTGGCCTATGGTCTGGAGTCAGGGTCCAAGTAGTAGTGCAGAGGGTGTACTCTCTTCTCAGCA
  GAGATGGTGCCAGGAGCCAGCAAGGGCACACAGTGGGACATGCAGATGCCTCTGGAGCATGGTCCTGCCT
  TCTAGTGCAGGATGCCTGTGGCATGTTCGGGGAAGTGTTCTTGAACAGCACCCTCTGGAAGAGCAGACAG
  TTAGAAGGGAAGTCCTGCAGCATGTCGGGAGTGAAGGTTCTACAGAAAGCTACAAGAGGAAGGACACCAG
  GGCTCTTCAGTTTAATTGACAGTTTGTGGCCCCCAGTGATATCTCTGACTGAGCCTTCCTGTGGCCAGCC
  CAGTGGAGAGACAAAGACTTATCTGCCTCCTCCTATCTTCTGTTATATCTTCTCTGCACATCCAACACTG
  GGACAAATTGATGCAATTGAAGACCACATTTCCAAGCTTTACCAGCCTCCAGTTGTCCGCTGCTTAAAAG
  AAATCCTCCAGACTAATGAGTGCAGTACACGTTGCAGCTTCTATGCCAGAGTGATTTATCAAAAACCACA
  GCTAAAGAATCTTTTGGCGCAAAAGGAAATCTGGCTGCTGGTGACTGACATCACTCTGCAAACACAGGAT
  GAAAGGGACCACTCTCTTCCCAAAACCCTGCCTGTCTATATAGCCCCCTCGTGTGTGCTGGGCCCAGAGG
  TTGTAGAAGAACTTGCCTTGCTGGTGTCTTATAACTTACTCTTCAGGGACGCTTTTAAGGACAACGGTCA
  GATTGTTTGTATTGAACGGACAGTCATCCTACCCCAGAAACCTCTCTTGTGTGTACCTTCTGCTTCCTGT
  GACTTGCCCAGCCCAGTGACACTGGATGAACTGAGTGCCCTCACACCTGTCAACTCCATTTGCAGTGTGC
  AAGGTACTGTGGTTGATGTAGATGAGAGCACTGCTTTCTCGTGGCCTGTGTGTGACCGGTGTGGCAATGG
  GAGACTGGAACAGAAGCCAGAAGATGGAGGCACCTTCTCCTGTGGGGACTGTTCCCAACTGGTCCTGTCA
  CCTCTTCAAGAGAGGCACCTGCATGTCTTTCTGGACTGTCCCACGAGACCTGAGAGTACTGTGAAGGTCA
  AGCTATTGGAGAGCAGTATTTCTTTACTTCTGATGTCTGCTGCCTCTGAGGATGGGAGTTATGAAGTGGA
  GAGCGTCCTTGGGAAGGAGATGGGACCTTTACTCTGCTTTGTCCAGTCCATCACTACACAGCAGAGCAGC
  TGTGTCGTCACCCTGGAAGAGATTGAGCTGCTGAGTACAGAGGGAGCCACTGCTGCTCAGCCTCCTCCCT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146068
• Insert Size: 2802 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146068.4, NP_666180.2
• RefSeq Size: 3303 bp
• RefSeq ORF: 2802 bp
• Locus ID: 224008
• UniProt ID: Q8BGX7
• Cytogenetics: 16 A2
• Gene Summary: Plays a role in DNA double-strand break (DBS) repair via homologous recombination (HR). Serves as a scaffolding protein that helps to promote the recruitment of DNA-processing enzymes like the helicase BLM and recombinase RAD51 to site of DNA damage, and hence contributes to maintain genomic integrity (By similarity).[UniProtKB/Swiss-Prot Function]