AF529169 (NM_153509) Mouse Untagged Clone

CAT#: MC222610

AF529169 (untagged) - Mouse cDNA sequence AF529169 (AF529169), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: AF529169
• Synonyms: DD1; Kiaa1024
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222610 representing NM_153509
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCAATCAGGAAGCTTCCCTCTTTTTGGTGAAGATCTTAGAAGAACTGGACAGTAAACAAAATA
  CTGTTTCCTATCAAGACCTGTGCAAGTCACTCTGTGCCCAGTTTGATCTGTCTCAGCTTGCCAAACTGAG
  AAGTGTGCTTTTCTATACAGCATGCCTCGATCCCAACTTTCCAGCAACTTTATTCAAAGACAAGATGAAA
  TGCTCTGTAAATAATCAGCAGTCAAAGAAAATCATGGTGGCAGCAGATATCGTAACAATCTTCAACCTGA
  TCCAGATGAATGGGGGCACTGCCAAGGAGAAGCTGCCCATGAGTTGCCACAAGGTGCGCAAGAAGGAAGC
  ATCCTTTGAGTCTTGCCGGTCCGACACCGAGGTGTGCAGCCCGACAGTTTGTGAGCCTCTGAACTGTGAG
  CTGAGTGAGAGACCCTTCAGCCGAGGCTACCCCACCAGGCAGTCATCCAAATGCCGGAAGATGGACTGTA
  AGGAATGCCCACAGTTTGTCCCTGCCTCTGAGCCCAACTTCCTGTTGGGTGTTAGCAAAGAGGTGAAAAA
  TCGGGCAGCCTCTCTGGATAGGCTGCAAGCCCTGTCCCCCTATTCTGTGGCCAGCCCTCAGCCCTGTGAG
  ATGCAGAGGACCTATTTCCCCATGAATATTGAGAACGAGCCCATGTCAGATCAGGACTCTCTGCCCATCA
  GCCAAGGCATCAAAGAGACCTTCATCTCCAGTGAGGAACCGTTTGTGGTTCAGTCCTGTGTTCAGAAAAG
  GAACATCTTCAAAGAAGACTTCCACAATCTCATGACTGTATCCCCCAGTTTGGTTGGTACAACTAACAAA
  GCAGAAGAGGGTCATGGGGAGCCCCAGAGTCAGAAGGAGCTCCACAAGCCACCCTTCTTCAACCACAGCT
  TTGAGATGCCTTACCACAACCAATACCTGAATCCGGTGTATTCCCCCATCCCTGACAAAAGGCGGGCGAA
  GCATGAAAGCTTAGATGACCTTCAAGCCTCTACCTATTTTGGACCCACTCCAGTGATGGGAACACAAGAT
  ACCAGGCGCTGTCCAGGGAGGTCCAGTAAACAGACTCCATGGCCCGCCAAAAGCTGGAGCCTAAACACGG
  AGGAAGTGCCTGATTTCGAACGCTCCTTTTTCAATAGAAATCCCTCTGAAGAGAAGCTACGCTATCCAAA
  TTCTGGCAGCCAGACTCCCAATTTCTCAGGCCCAGACAGGCATCCAGTTTACTTGGTGCCAAAGGATCAA
  CAGAAGGTTCTCCCTGCTGGCTATGCAGTGAAACCAAATGGACTCAAATCGAAAGAGATCTCGTCCCCTG
  TTGACCTAGAGAAGCACGAAGCAGTCAAGAAGTTTAAAGACAAAAGCATCAGCTGCACCAGTGGGCAGCA
  CAGCTCAGACACCAGTAGTGTGGGTACCCAGACAGAGCAGCATGTGCTGGATCCCCCCAAATGCAAAGAT
  TTGTGTACTTCGGGTCAGGCCAAGTACGGTGACAGGCATGCCATGAAGCAGTCGGACGATGACTCAGAAA
  TCGTCAGCGACGACATCAGTGACATTTTCCGGTTTCTCGATGACATGAGCATCAGCGGCTCCACAGGAGT
  GATACAGTCATCCTGCTACAACAGCACCGGCTCCTTGTCTCAGCTTCATAAGTCAGACTGTGATAGTTCG
  CCAGAGCACCACCTCGCCAAAATCACCAATGGGGTCTCCAGCGGCAAGGGGGACAAGTGTAACCGGCCTG
  AAAACGTCCATCACTCCGAGGAAGAGTTGAAGAGTAGCGTGTGCAAACTGGTTCTCAGGATTGGTGAGAT
  AGAGCGGAAGCTGGAATCGCTGTCTGGGGTCCGAGAGGAAATCTCCCAGGTCCTTGGGAAACTCAATAAA
  CTGGATCAGAAAATACAGCAGCCGGAGAAAGTGAATGTGCAGATTGACCTGAACTCCTTGACGAGTGAGG
  CACCTTCTGATGACAGCGCCTCTCCGAGGGTCTTCCGTGCACACAGTGGCTCCCACGGACCTAAGCTGGA
  GAACAGTCCTGACTGGTGCTGCTCAGATGCCAGTGGAAGTAACAGTGAAAGCCTGCGTGTCAAGGCCTTA
  AAGAAAAGCCTCTTTACCAGGCCATCTTCCAGGTCCCTGACTGAGGAGAACAGTGCCACAGAGTCTAAAA
  TTGCCAGCATCTCGAACTCTCCCAGAGACTGGCGAACCATCACATACACCAACCGGATGAGCCTCAACGA
  GGAGGAGATAAAAGATGCAGGCCCAGCCAATAACAAAGATTGGCATCGGAAATCTAAGGAGGCAGACAGG
  CAGTATGACATCCCCCCACAGCACAGATTGCCCAAGCAGCCCAAAGATGGCTTCCTGGTGGAGCAGGTGT
  TCAGCCCTCATCCTTACCCCACCTCCCTCAAGGGCCACATGAAGAGCAACCCCCTGTATACAGACATGCG
  GCTGACTGAACTAGCTGAGGTGAAGCGGGGCCAGCCTTCTTGGACCATAGAGGAATATGCTCGGAACTCC
  GGTGACAAAGGCAAGCTGACAGCTCTGGACCTGCAGACCCAAGAATCTTTAAACCCAAACAACTTAGAGT
  ACTGGATGGAAGATATTTATACTCCAGGCTATGATTCATTACTAAAACGGAAAGAAGCTGAATTCAGACG
  AGCCAAGGTTTGCAAGATCGCTGCTCTTATCACTGCTGCTGCATGCACGGTCATTCTTGTCATTGTTGTG
  CCAATCTGCACGATGAAGTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153509
• Insert Size: 2754 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153509.2, NP_705729.1
• RefSeq Size: 4250 bp
• RefSeq ORF: 2754 bp
• Locus ID: 209743
• UniProt ID: Q8K3V7
• Cytogenetics: 9 E3.1
• Gene Summary: Intrinsically disordered protein which may negatively regulate mTOR signaling pathway by stabilizing the mTOR complex component DEPTOR. Negatively regulates angiogenesis. Negatively regulates cell growth (By similarity). Negatively regulates neurite outgrowth in hippocampal neurons (By similarity).[UniProtKB/Swiss-Prot Function]