Kcnb2 (NM_001098528) Mouse Untagged Clone

CAT#: MC222541

Kcnb2 (untagged) - Mouse potassium voltage gated channel, Shab-related subfamily, member 2 (Kcnb2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnb2
• Synonyms: 9630047L19Rik; BB130875; Kv2.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222541 representing NM_001098528
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAAAGGCACCACCTGGCTTGAACAGAAAGACATCCAGGTCAACACTTTCTCTTCCTCCAGAAC
  CTGTGGACATTATCCGAAGCAAAACATGCTCTCGGAGAGTTAAGATCAACGTAGGGGGCCTTAACCACGA
  AGTCCTGTGGAGAACTCTGGACAGGCTTCCCCGGACACGCCTGGGGAAGCTCCGCGACTGTAACACTCAC
  GAGAGTCTCCTGGAGGTGTGCGATGACTACAACCTTAACGAGAATGAGTATTTCTTCGACCGACATCCTG
  GAGCCTTTACATCTATTCTAAACTTCTACCGGACGGGGAAGCTCCACATGATGGAGGAAATGTGTGCGCT
  CTCTTTTGGCCAAGAGCTGGATTATTGGGGCATCGATGAGATCTACCTAGAGTCCTGCTGCCAAGCCAGA
  TACCATCAGAAGAAAGAGCAGATGAACGAAGAACTGAGGAGGGAGGCGGAGACCATGCGTGAGCGTGAGG
  GTGAGGAGTTTGATAACACCTGCTGCCCGGAGAAACGGAAGAAACTTTGGGACTTGCTGGAGAAACCTAA
  CTCGTCCGTGGCTGCAAAGATCCTGGCCATCGTGTCTATCCTGTTCATCGTTCTTTCCACCATTGCTCTG
  TCTCTCAACACACTTCCAGAGCTGCAAGAAAACGACGAGTTTGGGCAACCCAGTGACAACCGGAAGTTGG
  CACACGTAGAGGCTGTGTGCATTGCCTGGTTTACCATGGAGTATCTTTTACGATTCCTGTCCTCACCGAA
  TAAATGGAAATTCTTTAAAGGCCCGCTGAATGTCATTGACCTTCTGGCCATCTTGCCATACTATGTAACC
  ATCTTCCTCACCGAGTCTAACAAAAGTGTGCTGCAGTTCCAGAATGTTAGACGAGTGGTCCAGATCTTCA
  GAATCATGCGTATCCTCAGGATACTGAAACTCGCCAGACACTCAACGGGCCTGCAGTCTCTGGGCTTCAC
  ACTCAGGCGAAGTTACAATGAGTTAGGTTTATTAATATTATTTCTGGCCATGGGGATAATGATATTTTCC
  AGCTTGGTGTTTTTTGCTGAGAAAGATGAAGATGCTACCAAATTCACCAGTATCCCTGCATCATTTTGGT
  GGGCTACCATCACCATGACCACTGTGGGCTACGGTGACATTTACCCTAAAACACTGTTAGGAAAAATTGT
  GGGTGGTCTCTGCTGCATTGCAGGAGTCCTGGTTATTGCCCTTCCTATACCCATCATTGTGAACAATTTC
  TCTGAGTTCTATAAGGAGCAAAAACGCCAGGAGAAAGCTATTAAAAGGAGAGAGGCTCTTGAAAGAGCCA
  AAAGAAATGGCAGCATCGTTTCTATGAACTTAAAGGATGCCTTTGCTAGAAGTATGGAACTGATAGACGT
  GGCTGTGGAGAAGGCTGGAGAGTCAGCCAATACCAAGGACTCAGTAGATGATAATCACCTGTCTCCAAGC
  CGGTGGAAGTGGGCCAGGAAGGCGCTGTCAGAAACGAGCTCCAACAAATCTTACGAGAATAAGTACCAGG
  AGGTTAGCCAAAATGACTCCCACGAACATCTGAACAATACTTCTTCCTCCAGCCCACAGCATCTGAGTGC
  CCAGAAGCTGGAGATGCTATACAATGAAATCACCAAGACACAGCCTCATTCCCACCCGAATCCAGACTGC
  CAAGAACAGCCTGAGAGGCCATGTGTGTATGAGGAGGAGATAGAAATGGAAGAGGTGATCTGCCCACAGG
  AGCAGCTGGCTGTGGCACAGACCGAGGTCATCGTGGACATGAAGAGCACCTCCAGCATTGACAGCTTCAC
  CAGCTGTGCCACGGACTTCACAGAGACTGAGAGATCACCCCTGCCACCACCCTCTGCCTCTCATTTGCAG
  ATGAAGTTCCCCACGGACCTCCCAGGAACAGATGAGCACCAAAGAGCCAGGGCACCTCCATTCCTTACAC
  TAAGCAGAGATAAGGGGCCTGCTGCCAGGGAGGCTGCAGTGGATTATGCCCCAATTGACATAACCGTGAA
  CCTCGATGCTGGGGCTTCCCATGGTCCCTTGCAACCTGACAGTGCCAGCGACAGCCCTAAGAGCTCGCTG
  AAAGGGAGCAATCCCCTCAAGTCAAGATCCCTCAAAGTGAACTTTCAGGAAAACAGAGCCAGTGCACCAC
  AGACCCCGCCCAGCACAGCCAGGCCACTGCCAGTAACCACAGCTGACTTTCCGCTCACCACTCCCCAGCA
  CATGAGTACCATTCTTCTAGAAGAAGCCCTCCCCCAGGGACAGCCTCCCTTGCTGGAGGCTGATGATTCG
  GCACACTGTCAGGGACCATCCAAAGGTTTCTCCCCACGATTTCCCAAGCAAAAACTGTTTCCTTTCTCTT
  CTAGAGAAAGGAGGAGCTTCACTGAAATAGACACTGGAGAAGACGAAGACTTCTTGGACCTCCAAAGGTC
  AAGACCAGACAAGCAAGCAGATCCCAGCCCCAACTGCCTAGCAGATAAGCCTGGCGATGCCAGAGACTCT
  TTAAGAGAAGAGGGCTGTGTGGGTTCCTCCTCTCCCCAGAACACAGACCACAACTGTAGGCAAGACATTT
  ACCAGGCTGTGGGTGAAGTCAAAAAGGACAGTAGTCAAGAAGGTTACAAGATGGAGAACCACTTGTTTGC
  CCCAGAAATTCATTCCAACCCAGGAGACACAGGTCACTGTCCCACTCGTGAGACCAGCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098528
• Insert Size: 2724 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098528.2, NP_001091998.1
• RefSeq Size: 5309 bp
• RefSeq ORF: 2724 bp
• Locus ID: 98741
• UniProt ID: A6H8H5
• Cytogenetics: 1 4.5 cM
• Gene Summary: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and smooth muscle cells. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient. Homotetrameric channels mediate a delayed-rectifier voltage-dependent outward potassium current that display rapid activation and slow inactivation in response to membrane depolarization. Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNB1; channel properties depend on the type of alpha subunits that are part of the channel. Can also form functional heterotetrameric channels with other alpha subunits that are non-conducting when expressed alone, such as KCNS1 and KCNS2, creating a functionally diverse range of channel complexes. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Contributes to the delayed-rectifier voltage-gated potassium current in cortical pyramidal neurons and smooth muscle cells.[UniProtKB/Swiss-Prot Function]