Itih1 (NM_008406) Mouse Untagged Clone

CAT#: MC222516

Itih1 (untagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (Itih1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itih1
• Synonyms: In; Intin1; ITI-HC1; Itih; Itih-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222516 representing NM_008406
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGTGCCACAGGGCTCCGGGTGCTGCTGTGCTTGCTGCCGCTCCTCACTCTGCAGGCCAGGCCTG
  CTTTGGGCTTGGCAACAGGCAGACCCAGGGGCAGTGAGAAGCGACACGCTGTGGACACTTCAGTCAATGG
  TGTGTCCATCAAGAGCTTGAAAGTCAATTGCAAAGTCACCTCTCGCTTTGCCCACTACGTCATCACCAGC
  CAAGTGGTCAACAATGCTGATAAAGCCAGGGAAGTGGCTTTTGATGTGGAAATCCCCAAGACAGCCTTCA
  TCAGTGACTTTGCCATAACATCAGATGGGAAGGCGTTCATTGGGGACATAAAGGACAAAGTGACTGCATG
  GAAGCAGTACCGGAAGGCAGCCGTTTTAGGGGAGAGCGCTGGCCTTGTCAGAGCCTCGGGCAGAAATATG
  GAGCAGTTCACCATCCATATCACTGTTGGAGCCCAGAGCAAGGCCACGTTCCGGCTCACCTATGAGGAGG
  TACTAAAGAGGAGACTGATGCAGTATGACATCACCATCAAAGTCAGACCCAAGCAGCTGGTACAGCATTT
  TGAGATCGATGTGGACATCTTTGAACCCCAGGGGATCAGCAAGCTGGACGCTCAGGCCTCCTTCCTCAGT
  GAGGAACTGGCTGCTCAAACCATCAAGAAGTCTTTCTCAGGGAAAAAGGGTCATGTGCTCTTCCGCCCCA
  CTGTGAGCCAGCAGCAGTCCTGCCCCACATGTTCTACATCCTTGCTAAATGGAGAATTCAAGGTGACCTA
  CGATGTCAATCGAGACAAGCTCTGTGACCTCCTGGTGGCCAACAACTACTTTACACATTTCTTTGCCCCC
  AAAAACCTGACCAACATGAGCAAGAACCTGGTTTTTGTGATTGACATCAGTGGCTCCATGGAAGGCCAGA
  AAGTGAGGCAGACAAAGGAGGCACTCCTTAAGATCCTGGAGGACATGAGGCCAGTAGACAACTTTGACCT
  GGTCCTCTTTGGCTCTAAAGTGCAATCATGGAAGGGCTCCCTGGTACCTGCGTCTAATGCCAATCTGCAA
  GCAGCTCAAGACTTTGTGAGGCGCTTTTCTTTGGCTGGAGCCACAAACCTGAATGGAGGCTTGCTCCGAG
  GAATCGAGATCTTAAACAAAGCTCAAGGAAGCCACCCAGAACTCAGCAGCCCGGCCTCAATTCTTATCAT
  GTTGACAGACGGAGAGCCCACTGAAGGGGAGACGGACCGTTCCCAGATCCTCAAGAATGTACGCAACGCT
  ATCCGGGGCAGGTTCCCGCTCTACAACCTCGGCTTTGGCCACGACCTGGACTTCAGCTTCCTGGAGGTCA
  TGTCCACTGAGAACAACGGATGGGCCCAGAGAATTTATGAGGACCATGATGCCACCCAACAGCTACAGGG
  TTTCTACAATCAAGTAGCCAATCCCCTGCTGACAGATGTGGAACTGCAGTACCCCCAGGATGCTGTCTTG
  GCTCTAACCCAGCACCGACATAAACAGTACTATGACGGCTCGGAGATTGTGGTGGCTGGGCGCATTGCTA
  ACCACAAACTGAACACCTTTAAGGCTGACGTTCGGGCTCGTGGGGAAAAGCAAGAATTCAGGGCAACCTG
  CCTAGTGGACGAGGAAGAGATGAAGAAACTGCTCCGAGAGCGTGGCCACGTGCTAGAGAATCATGTGGAG
  AGGCTCTGGGCCTACCTCACGATCCAGGAGCTCCTGGCTAAGCGGATGAAGACGGAAGGGGAGGAGAGGG
  CCAACCTGTCATCCCAGGTCCTGAAGATGTCACTGGACTATCATTTCGTAACGCCACTGACCTCTCTGAC
  TATCAGAGGCCTGACAGACGAGGATGGGCTGGAGCCCACCATTGACAAGCCCCTGGAGGATTCTCAGCCC
  TTAGAGATGGTGGGACCCAGAAGAACGTTTGTGCTGTCAGCCATACAGCCATCTCCTACAGCCCACCCCA
  TAGACTCAAAGTTGCCACTCCGTGTGACAGGTGTGGACACCGATCCCCACTTCATCATCTATGTGCCCTC
  AAAAGAGGATAGCTTGTGCTTCAACATCAATGAGGAACCTGGTGTGATCCTGAACCTAGTACAGGACCCT
  GACACAGGCTTCACCGTGAATGGGCAGCTCATTGGGAACAAGGCCAGCAGCCCTGGGCAACACGAGAGCA
  CATACTTCGGGAGATTGGGAATCTCAAGTCCCACATCAGACTTTCAGCTGGAAGTGACTCCTCAGAACAT
  TACACTGAACCCCAGCTCCAGCGGGTCCATGTTCTCCTGGAGGGACCAGGCTGTGCTGCAGAAGGATGGG
  GTAGTGGTGACAATCAACAAGAAGAGGAACCTGGTGGTGTCTGTGGATGATGGGGCTACCTTTGAGATTG
  TCCTGCACAGAACATGGAAGGGGAGTGCAGTCCACCAGGACTTCCTGGGTTTCTATGTGCTGGACAGCTT
  CCGGATGTCAGCCCGGACAAAGGGGCTACTGGGACAATTCTTCTCCCCACTGGATTTTGAAGTATTTGAC
  CTCCACCCAGGCTCTGACCCTACAAAGACAGATGCCACAATGGTGGTGAAGAATCGGCAGCTGACAGTCA
  CCCGAGGGTTGCAAAAAGACTACAGCAAGGACCCCAGACATGGAGCAGAAGTGCCTTGCTGGTTTGTCCA
  CGACAATGGAGCTGGACTGATTGATGGTGTTCACACTGACTATGTCGTCTCTGACATCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008406
• Insert Size: 2724 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008406.3, NP_032432.2
• RefSeq Size: 3077 bp
• RefSeq ORF: 2724 bp
• Locus ID: 16424
• UniProt ID: Q61702
• Cytogenetics: 14 B
• Gene Summary: This gene encodes a heavy chain of inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors. IaI proteins are protein-glycosaminoglycan-protein complexes comprised of two heavy chains and a light chain. The encoded protein covalently associates with the light chain via a chondroitin sulfate moiety. Intravenous administration of the encoded protein improved survival of mice after infection with Escherichia coli. This gene is located adjacent to two other IaI heavy chain genes. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature protein. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1).