Ddhd1 (NM_176845) Mouse Untagged Clone

CAT#: MC222393

Ddhd1 (untagged) - Mouse DDHD domain containing 1 (Ddhd1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddhd1
• Synonyms: Mir5131; PA-PLA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222393 representing NM_176845
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTACCCGGGCCGCGGCGCCCCACGGAGCCCCGAGCGTAACGGCCGCGGCGGCGGTGCCTGGGAGC
  TGGGCTCGGACGCGGCGGGCGTGTTCGGCGGCGGCGGCTGCTGCTTCGAGCACCAGCCGGGCGACAGCGT
  GCCGCTGTCGCTGCTGCGCGCCGAGCCGCTGCACCTGGCACCGGGCGCCGACGACCTGAGCCACCTGGCT
  CTGGACCCGTGCCTCAGCGACGAGACCTACGACTTCAGCTCGGCCGAGTCCGGCTCGTCGTTGCGCTACT
  ACAGCGAGGGCGAGAGCGCGGGCGGCGGCAGCTCTTCTTCGCCTCCGCCGCCGCTCGTCGCCCAGAACTC
  GGGGGGCGGCGGGGCGGCGGGAGGGGGCCCCGGGGACAGGAAGCGCGCCCGGCCCGGCGGCGCAGCCGCC
  CGGCACCGCTACGAGGTGGTGACGGAGCTGGGCCCCGAGGAGGTGCGCTGGTTCTACAAGGAAGACAAGA
  AGACCTGGAAGCCCTTCATCGGCTACGACTCTCTGCGCATCGAGCTCGCCTTCCGGACCCTGCTGCAGAC
  CACGGGCGCCCAGGCCCGGGCGGCGGGCCGCGATGGAGACCGGGTGTGTGGCCCAAGCTCCAGCTTCGGG
  GAGGAAGACGAGGAGGACAGCGCCTGCGGCTTCTGCCCGCGCGCGGCGGGGCCTGAGCCGGAGATGGAGG
  AGCTGGTGACCATCGAGCCGGTGTGCGTGCGCGGCGGCCTCTACGAGGTGGATGTCACTCAAGGAGAGTG
  CTACCCGGTGTACTGGAACCAAGCTGATAAAATACCAGTAATGCGTGGACAGTGGTTTATTGATGGGACG
  TGGCAACCTCTAGAAGAAGAAGAAAGTAATTTAATTGAGCAAGAACATCTCAGTTGTTTTAGGGGTCAGC
  AGATGCAAGAAAATTTTGATATTGAAGTGTCAAAATCCTTAGATGGAAAAGACGCTGTTCACAGTTTCAA
  GCTGAGTCGGAACCACGTGGACTGGCACAGCATGGATGAAGTCTATCTCTACAGTGACGCAACAACATCC
  AAAATTGCGAGAACCGTTACCCAAAAACTGGGCTTTTCTAAAGCCTCCAGCAGTGGGACCAGACTGCACA
  GAGGTTATGTAGAAGAAGCCACACTGGAAGACAAGCCGTCGCAGACTTCTCATATCGTGTTTGTTGTGCA
  CGGCATTGGACAGAAGATGGACCAAGGAAGAATCATCAAAAACACAGCCATGATGAGAGAGGCTGCAAGG
  AAAATGGAAGAAAAGCATTTTTCCAACCATGCAACACATGTTGAATTTCTGCCTGTTGAATGGCGATCAA
  AGCTTACTCTTGATGGAGACACAGTTGATTCCATCACTCCTGACAAAGTGCGAGGCCTGAGGGACATGCT
  CAACAGCAGCGCGATGGACATCATGTACTACACCAGCCCACTCTACAGGGATGAGCTAGTTAAAGGCCTT
  CAGCAAGAGCTGAATCGATTATATTCCCTTTTCTGTTCCCGGAATCCAGACTTTGAAGAGAAAGGGGGTA
  AAGTCTCAATAGTGTCCCATTCCTTGGGGTGTGTAATCACTTATGACATCATGATGGGCTGGAATCCAGG
  TGGACTCTATGAGCAGCTGCTGCAGAAGGAGGAAGAGCTGCCCGACGAGCGGTGGATGAGCTATGAAGAG
  CGGCATCTTCTTGACGAGCTCTATATAACAAAGCGACGGCTGAGGGAAATAGAAGATCGGCTGCACGGAT
  TGAAGGCGCCATCCATATCACAAACACCTGCCTTAAAATTTAAGGTTGAAAATTTCTTCTGTATGGGATC
  TCCGCTAGCAGTTTTTTTGGCATTACGCGGCATCCGACCAGGAAATTCTGGAAGTCAAGACCACATTTTA
  CCTAGAGAGATCTGTAACCGCTTACTAAATATTTTTCATCCTACGGACCCAGTGGCCTACAGATTAGAAC
  CGTTGATTTTGAAACACTACAGCAACATTTCTCCAGTCCAGATCCACTGGTACAACACTTCAAACCCTCT
  ACCTTATGAGCATATGAAGCCAAATTTTCTCAACCCAGCAAAAGAACCTACCTCAGTTTCAGACAGTGAG
  AACATAGCAGCTATCCCAAGCCCTGTGACCTCCCCCGTCTTATCTCGGCGCCACTATGGAGAATCTATAA
  CTAACATTGGCAAAGCAAGCATATTGGGGGCTGCTAGCATTGGAAAGGGCCTTGGAGGAATGTTGTTCTC
  AAGATTTGGACGTTCTTCAGCATCACAGCCGTCTGAGCCATCTAAAGACTCACTGGAAGATGATAAGAAG
  CCTTCAGCTTCACCCTCTACCACCACCGTGGCAACGCAGACCCTGCCGCACAGTGGCTCCGGCTTCCTCG
  ACTCCGCATATTTCAGACTTCAAGAATCGTTCTTTTATCTCCCACAACTTCTTTTTCCGGAAAATGTAAT
  GCAGAGTAAAGATGATAGCCTCGTGGAACTGGAACACAGGATCGACTTTGAACTCAGAGAAGGCCTTGTG
  GAGAGCCGCTATTGGTCAGCCGTCACGTCGCACACTGCCTATTGGTCATCCTTGGATGTCGCTCTTTTTC
  TTTTAACATTCATGTACAAACACGAGCATGATACCGAGGCCAAGCCCAGCCTAGGTTCACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176845
• Insert Size: 2655 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176845.5, NP_789815.3
• RefSeq Size: 5099 bp
• RefSeq ORF: 2655 bp
• Locus ID: 114874
• UniProt ID: Q80YA3
• Cytogenetics: 14 C1
• Gene Summary: Phospholipase that hydrolyzes phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid. The different isoforms may change the substrate specificity (By similarity). Required for the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 3. The encoded isoform (2) is shorter, compared to isoform 3.