Ddx10 (NM_029936) Mouse Untagged Clone

CAT#: MC222335

Ddx10 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx10
• Synonyms: 4632415A01Rik; AI646054
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222335 representing NM_029936
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAAACGGTTGCTTCTCTGGGTCAGGGAACCCGACCCGACCCGGTGCGGAGCTTTAATCGCTGGA
  AGAAAAAACACAGCCACAGGCAGCACCAAAAGAAGGAGCGGAGGAAGCAGCTAAAGAAGCCGGAATGGCA
  GGTCGAGCGCGAGGGGATCAGCCGCCTCATGCAAAACTACGAGAAGATAAATGTAAATGAAATTACAAGA
  TTTTCAGATTTCCCTTTGTCCAAAAAAACATTGAAAGGTTTGCAAGAAGCACAGTACCGCTTGGTAACAG
  AGATACAGAAGCAGACCATAGGGTTGGCTTTGCAAGGTAAAGATGTGCTTGGTGCTGCCAAAACTGGATC
  TGGCAAGACTTTGGCTTTTCTTGTTCCGGTGTTGGAAGCCTTATACCGCCTGCAGTGGACTTCAACAGAT
  GGCCTGGGAGTTCTGATAATATCACCTACTCGAGAACTGGCCTATCAGACATTTGAGGTTCTACGCAAAG
  TAGGGAAGAATCATGATTTTTCAGCTGGTCTCATCATTGGTGGGAAGGATCTGAAACATGAAGCTGAGAG
  GATCAACAACATAAACATACTGGTTTGCACACCAGGTCGACTTCTTCAACACATGGATGAAACAATATGT
  TTTCATGCCACCAATCTTCAGATGTTAGTTCTTGATGAGGCAGATAGAATCTTGGATATGGGCTTTGCTG
  ATACCATGAATGCTATTATTGAAAATCTTCCCAAGAAACGCCAGACTTTACTGTTCTCAGCCACACAGAC
  CAAATCTGTAAAGGACCTTGCGCGCTTGAGTTTGAAAGACCCTGAGTATGTCTGGGTTCATGAAAAAGCA
  AAATACAGCACCCCTGCTACGTTGGAACAGAACTATATAATTTGTGAGCTGCACCAAAAAATAAGTGTGC
  TGTTTTCCTTTTTGAGAAGCCACCTAAAGAAGAAGAGTATTGTGTTTTTCTCCAGTTGCAAAGAGGTTCA
  GTATCTGTATAGGGTGTTCTGCAGGCTGCGCCCAGGGATCTCGATCCTTGCTCTCCATGGCCGGCAGCAG
  CAGATGAGAAGAATGGAGGTCTACAATGAATTTGTCCGAAAGAGGGCCGCAGTACTTTTTGCCACTGATA
  TTGCAGCTAGGGGACTGGATTTTCCAGCTGTGAATTGGGTCCTTCAGTTTGATTGTCCAGAGGATGCCAA
  CACGTATATTCACAGAGCTGGTAGAACTGCCAGGTACAAAGAAGATGGAGAAGCGTTATTAATTTTACTA
  CCTTCAGAAGAGCAAGGAATGGTGCAGCAGCTTCTCCAGAAGAAAGTACCTGTGAAGGAGATCAAAATCA
  ATCCAGAAAAACTTATTGATGTTCAGAAAAAATTAGAATCATTTTTAGCTCAAGATCAAGATTTAAAAGA
  AAGAGCTCAAAGGTGTTTTGTCTCCTATATACGCTCAGTATATCTTATGAAGGATAAAGAAGTATTTAAT
  GTGAGCAAGTTACCTATTACTGAATATGCACTGTCTCTTGGTCTTGCTGTGGCACCGCGGATAAGATTTC
  TTCAGAAACTGGAGAAGCAGCCCAGCACAGAATTGGTAAAGAATCCAGTCACAGAAGCAGTTCCGCCCAG
  GGCCCCCTCCCTCCCCAACGATGAAGCAGACGAATCTCCAGCTTATGTCAGTGAGAAGATGTCCGTCCTT
  CACAAAAGTGGGGAAAGACTGGAAGAGACAGAACACAGACTGGCTAGTGGTGACGGTGACGAAGAACAGG
  ATGAAGAGACGGAAGACGAAGAGACGGAAGATCATCTGGGAAAAGCAAGAGAGCCTCACACTGAATCTGT
  TGTCAGTATTGAGGAGGCACAGAAGGTCAAGGAAGTTTCTGTGCAGTTCTTAAACAGAGATGATGATGAT
  GAAGATGGACCTGATGCTGACTTCCTCACAGTGAAGCGGCGGGATGTGTTTGGGTTGGATCTTAAAGAGA
  ATGAAGCACTGTCGAAAAAAGAACCTTCTAAATCCAGTGTCAAGAAAAAATTAACCAAAGTTGCAGAAGC
  AAAAAAAGTAATGAAAAGAAGTTTTAAAGTGAACAAGAAAATAACATTTACTGATGAAGGGGAGTTGGTT
  CAGCAGTGGCCACAGATTCAGAAATGTGCTATAAAAGATGTGGAGGAAGAAGATGACACTGGTGGTATCA
  ACCTAGACAAAGCAAAGGAAAGGCTTCAGGAAGAGGACAAATTTGACAAAGAAGAATACAGGAAGAAAAT
  TAAGGCAAAGCACCGGGAAAGACGGCTAAAAGAAAGAGAGGCCAGAAGAGAAGCCAACAAAAGACAAGCA
  AAGGCCAGAGATGAAGAGGAAGCCTTTCTGGACTGGAGTGATGAAGATGATGGTGGGTTTGATCCAAGTA
  CACTTCCAGATCCTGACAAGCACAGAAGTTCTGAAGAGTCGGAGAGTGAAGATACAAACCATAAGATGAG
  TGACACCAAGAAGAAGCAGGAAACAAGGAAGAGAAACAACACTGAAGACGATGATGTAAGACCAAGAAGT
  CGTCATGGAAAGAAGGCCAAGTGGGAAACGGTAGAACCTTTGGATACAGGCCTGTCTTTAGCAGAGGATG
  AAGAGCTGGTGTTACATCTCCTCAAAAGTCAAAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029936
• Insert Size: 2628 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029936.2, NP_084212.2
• RefSeq Size: 3368 bp
• RefSeq ORF: 2628 bp
• Locus ID: 77591
• UniProt ID: Q80Y44
• Cytogenetics: 9 A5.3
• Gene Summary: Putative ATP-dependent RNA helicase.[UniProtKB/Swiss-Prot Function]