Bclaf1 (NM_001025393) Mouse Untagged Clone

CAT#: MC222332

Bclaf1 (untagged) - Mouse BCL2-associated transcription factor 1 (Bclaf1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bclaf1
• Synonyms: 2610102K23Rik; 2700025J07Rik; 2810454G14Rik; 5730534O06Rik; AI450190; AW556225; Btf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222332 representing NM_001025393
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGCTCCAATTCTAGATCACATTCTTCAAGATCCAAGTCTAGATCACAGTCTAGTTCTAGATCAA
  GATCAAGATCACATTCTAGAAAGAAGAGATACAGTTCTAGGTCTCGTTCCAGAACATATTCGAGGTCTCG
  TAGTAGAGATCGTATTTATTCTAGAGATTATCGTCGAGATTACAGGAATAATAGAGGAATGAGACGACCT
  TATGGGTACAGAGGAAGGGGTAGAGGGTATTATCAAGGAGGAGGAGGGAGATACCATCGAGGTGGCTATA
  GACCTGTCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGGTCACGGTCCAGGAGTCCAAAAAG
  AAGATCCGTGTCTTCTCAAAGATCCCGAAGCAGATCTCGCCGGTCATATAGATCCTCTAGGTCTCCAAGA
  TCATCATCATCTCGCTCTTCATCCCCATATAGCAAATCTCCTGTCTCTAAAAGACGAGGGTCTCAGGAAA
  AACAAACCAAAAAAGCTGAGGGGGAACCCCAAGAAGAGAGTCCTTTGAAAAGCAAATCACAGGAGGAACC
  AAAGGATACTTTTGAACATGATCCATCTGAATCTATTGATGAGTTTAATAAATCTGCTACTTCTGGTGAT
  ATTTGGCCTGGCCTTTCAGCTTATGATAATAGTCCAAGGTCACCTCATAGTCCTTCACCTATTGCTACAC
  CACCCAGTCAGAGTTCATCATGCTCAGATGCCCCCATGCTTAGTACAGTGCACTCTGCCAAAAATACCCC
  TTCTCAGCATTCACATTCCATTCAGCACAGTCCTGAGAGGTCTGGATCTGGCTCTGTTGGAAATGGGTCG
  AGTCGATATAGTCCTTCTCAGAATAGTCCGATTCATCACATCCCATCACGAAGAAGCCCTGCCAAGACAA
  TCACACCACAGAATGCTCCAAGAGAGGAATCTAGGGGGCGGTCCTCCTTTTATCCTGAAGGAGATCAGGA
  AACAGCAAAGACAGGAAAGTTTTTAAAAAGGTTCACAGATGAAGAGTCTAGAGTATTCCTGCTTGATAGG
  GGTAATATCAGGGATAAAGAGGCTCCGAAGGAGAAAGGGTCAGAGAAAGGCAGGGCAGATGGAGATTGGG
  ATGACCAGGAAGTTCTAGATTACTTTAGTGATAAAGAGTCTGCAAAACAAAAATTCCATGACTCTGAAGG
  GGATGACACAGAGGAGACAGAGGATTATAGACAGTTTAGGAAATCAGTCCTAGCAGATCAGGGCAAGAGC
  TTTGCTACTTCATCTCACCGGAATACTGAGGAGGAAGGACCCAAGTACAAGTCCAAAGTTTCATTAAAAG
  GCAATAGAGAAAGTGATGGATTTAGAGAAGAAAAAAATTATAAACTGAAAGAGACTGCATACATAGTCGA
  AAGGCCTAGCACTGCAAAGGACAAGCACAAGGAAGAAGACAAAGGCTCTGATAGAATAACAGTGAAGAAA
  GAAGTGCAGTCACCGGAGCAGGTAAAGTCTGAAAAGCTCAAAGAGCTCTTTGATTACAGTCCCCCTCTAC
  ACAAGAGTCTGGATGCACGAGAAAAGTCCATCTTCAGAGAGGAGAGCCCACTGAGGATCAAAATGATAGC
  CAGTGATTCTCACCGTCCTGAAGTCAAACTCAAAATGGCACCTGTTCCTCTTGACGATTCCAACAGACCT
  GCCTCCTTGACTAAAGACAGGCTACTTGCTAGCACGCTTGTCCATTCTGTCAAGAAAGAGCAAGAATTCC
  GATCCATCTTTGACCACATTAAGCTGCCTCAGGCCAACAAAAGCACTTCAGAATCATTTATACAACACAT
  TGTATCCTTGGTTCATCATGTGAAAGAACAATACTTCAAATCACCTGCAGTGACCCTAAACGAGCGGTTC
  ACTTCATACCAGAAAGCTACTGAAGAACATAGTACCCGGCAAAAGAGCCCCGAGATACACAGGAGGATTG
  ACATCTCTCCCAGCGCTCTGAGGAAGCACACCCGGCTAGCAGGAGAGGAGAGAGGTTTTAAAGAAGAAAT
  TCAGAAGGGAGATAAAAAGTTAAGGTGTGATTCGGCTGACCTTCGGCACGACATTGACCGTCGCCGTAAA
  GAGAGAAGTAAAGAGCGGGGAGATTCCAAGGGCTCCAGGGAGTCCAGTGGGTCCAGAAAGCAAGAAAAAA
  CACCAAAGGATTACAAGGAGTATAAACCTTACAAAGATGACAGCAAACATAAAGGTAGAGAGCGGGACCA
  TTCCCGGTCATCGTCCTCGTCAGCATCGCCGTCCTCGCCCAGTTCTCGAGAAGAAAAAGAGAGTAAGAAA
  GAGAGAGAAGAAGAATTTAAAACTCACCACGAGATGAAAGACTACTCAGGATTTGCAGGAGTTAGCAGAC
  CACGAGGAACCTTTCACGATGACAGAGATGATGGTGTGGATTACTGGGCCAAAAGAGGAAGAGGTCGTGG
  TACTTTTCAACGTGGCAGAGGGCGCTTTAATTTCAAAAAATCAGGTAGCAGTCCAAAATGGACTCATGAC
  AAATACCAAGGAGATGGGATTGTTGAAGATGACGAAGAGACCATGGAAAATAATGAAGAGAAGAAGGACA
  GACGCAAAGAAGAAAAGGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025393
• Insert Size: 2613 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025393.1, NP_001020564.1
• RefSeq Size: 5307 bp
• RefSeq ORF: 2613 bp
• Locus ID: 72567
• Cytogenetics: 10 A3
• Gene Summary: Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region, as compared to variant 1. The encoded isoform 3 lacks an internal segment, as compared to isoform 1.