Poln (NM_181857) Mouse Untagged Clone

CAT#: MC222293

Poln (untagged) - Mouse DNA polymerase N (Poln), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Poln
• Synonyms: POL4P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222293 representing NM_181857
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGGAAAATTATGAGGCATGTGTAGGTTTTGATGTCTGTGAGATACCCCTCTCTGCTGTTGCTC
  AGAAGATTATGTCTGCTATGCGTTCAGGTGATTTCATGGATTCTAGGAATGAGGGAGAGAGTACAAACAC
  TTCAAAAGTGGCAAAGAAATCCAGTGTTCATTACTCAGTATTAGCTGAACATGAGGAGACTCAATCACTA
  GGAACAAAGAACCCCGAGTCATTAATAACCCAGACACCAAGAGGATCCATTGAGCTCTGTCCTCAGCCCT
  CCATTACTAAGCTCACATGTCAGCTCTCTGCTGGTCAAGTACAGAACAGCATCAGCTCATTGGGTCTTTC
  CAGTTATTTAATTCCACAGTGTGATCAGGAAGCTTCAGTTTTACCGAACATGGAGCATAAGAGACAGCAT
  TTCCTAAAGGAAAATATAGGCAAAGAAGATAAGGACAACTCGAGTCTTAAAAGAAAATATATTACATGTA
  GTAAATCCTCAGAAAAAGCAAGTAAACACACAGCTCTGGAAAAAGATACTGATGGGACTGAATCCTGGCC
  AAACTCCAGGGACACAAGAGCTTTGGGGGAAAGGCTGTGTGATGTTAGATATTTGGGTGATTTGGCCAAA
  GCCCAGCTGATGGATGCTCTCAAGCAGGCAGCAGCCCTGGTGGTGACATTGATGTACAAGGATGGTTCCA
  CACAGCTGAGCGCCAAGGAGGCTTTAACTTGTACTGTTAAAGGAATTGTAGTGTTACTAAAAAGCCATGT
  AGGCAACAGCACTCTGACTCTCCCAGCCCATGGTGGTGCTCTGGAAAAGGACTTCATATCCGAAGATCAC
  TGTGTCTACATACATACGGAGCATTCCCCTTTCTGGGACCCAAAGCAAGAAGCACATAGTCTATTTGTCA
  GGAACATACTGTTTTGGACACTGAGGTGTAAATGTCCCGTTGTTTGTTTTAATGCCAAGGACTTTGTGAG
  AACAGTGCTGCAGCTCTATGGTGAAGATGGCAGTTGGAAGCATGTTGCTGATTTTGTAGGACTAGATCCC
  AGAGTTGCTGCATGGCTCATAGACCCCAGTGACACTGCACCTTCATTTGAAGATTTAGTAGCAAAACACC
  TAGAAAAGTCCATCACAGTCAAACCAAGCAGCACATTCAGAGAAGCCTCAAGAAATACTTTGAGTCAGAA
  TGTATTTATGAATCTGAAGATACTCTACGACCTTACAATGGATCTTTGTTCTAAGTTGAAGGCTTATGGC
  TTATGGCAGCTATTTTGCACTTTGGAGCTTCCATTGATACCAATTTTGGCAGTGATGGAAAACCATAAGA
  TTCCAGTTGACAAAGAAGAAATGGAGAGGACATCGGCACTCCTCGGGGCTCGCCTCAAGGAATTGGAGCA
  AGAAGCCCATTTTGTTGCAGGAGAACAGTTTCTTATAATGAGTAATAATCAACTTCGTGAGATCCTCTTT
  GGCAAGTTGAAGCTGCACTTGTTGAGTCAGAGGAAACATCTTCCCAGAACTGGGCTGCAGAACCAGCTGT
  CCACATCAGAAGCCATGTTAAATTCTCTACAAGACCTTCACCCATTACCCAAGTTAATTTTGGAATATAG
  ACAGGTTCACAAGATCAAGTCAACTTTTATAGATGGATTACTAGCTTATATGAAAAAGGGCTCCATCTCT
  TCTACATGGAATCAGACTGGAACTGTGACAGGGAGACTTTCAGCCAAGCATCCAAATATCCAGGGTATCT
  CCAAGCACCCAATTAAGATTTCTAAGCCTTGGAATTTTAAAGGTAAAGAAGAGGAGACTGTCACCATCTC
  ACCAAGAACCCTGTTTGTGTCCTCTGAAGGCCACACCTTCCTGGCAGCAGACTTTTCACAGATTGAATTG
  CGCATTCTTGCACATTTATCTGGGGATCCAGAGCTTTTGAAGTTATTCCAGGAATCTGAAAGAGATGATG
  TATTTTCTACCCTGACTTCACAGTGGAAGGACATTCCCATAGAGCGTGTGACACACATGGACAGAGAACA
  AACCAAGAAAGTGGTGTACTCCGTGGTCTATGGAGCTGGGAAAGAGCGGCTTGCTGCTTGCCTTGGAGTC
  ACTGTTTTGGAAGCCACCCACTTTTTGGAGAGATTTTTGCAGAAGTACAAGAAAATCAAGGACTTTGCCC
  AGACAGTTATTGGCCAGTGTCACAGTGCAGGCTATGTGACATCCATCTTGGGTAGAAGGAGGCCTCTCCC
  GAGGATCTGTGCACAGGACCAGCAGCTTCGGGCACAAGCAGAGCGACAGGCAGTAAACTTTGTGGTACAA
  GGCTCAGCCGCAGACCTCTGTAAGCTAGCCATGATTCGCATCTCTACTGCAGTAGCCACATCCCCGACCT
  TGACAGCCAGGTTAGTTGCCCAAATTCATGATGAGCTGCTGTTTGAAGTAGAAGACACTCAGGTCCCTGA
  GTTTGCAGCTCTTGTCAGAAGGATCATGGAGTCCTTGCAACAGGTACAGACCCTGGAACTGCAGCTGCAG
  GTGCCCCTAAAAGTGAACTTGAGTGTTGGCCGCTCATGGGGACACCTGACCCCACTGCAGGAGATCCTGG
  GCTCAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181857
• Insert Size: 2601 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181857.4, NP_862905.1
• RefSeq Size: 2994 bp
• RefSeq ORF: 2601 bp
• Locus ID: 272158
• UniProt ID: Q7TQ07
• Cytogenetics: 5 B2
• Gene Summary: DNA polymerase with very low fidelity that catalyzes considerable misincorporation by inserting dTTP opposite a G template, and dGTP opposite a T template. Is the least accurate of the DNA polymerase A family (i.e. POLG, POLN and POLQ). Can perform accurate translesion DNA synthesis (TLS) past a 5S-thymine glycol. Can perform efficient strand displacement past a nick or a gap and gives rise to an amount of product similar to that on non-damaged template. Has no exonuclease activity. Error-prone DNA polymerase that preferentially misincorporates dT regardless of template sequence. May play a role in TLS during interstrand cross-link (ICL) repair. May be involved in TLS when genomic replication is blocked by extremely large major groove DNA lesions. May function in the bypass of some DNA-protein and DNA-DNA cross-links. May have a role in cellular tolerance to DNA cross-linking agents. Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with HELQ helicase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).