D330045A20Rik (NM_175326) Mouse Untagged Clone

CAT#: MC222068

D330045A20Rik (untagged) - Mouse RIKEN cDNA D330045A20 gene (D330045A20Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D330045A20Rik
• Synonyms: AI132325; Radx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222068 representing NM_175326
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGTGAGTCGGGTCAGCCTCAGCCGGGACCCTCGCACGCTGGTCTCTACTTGGAGCACCCCGAAA
  GGGATCAGGCTGGGGTCCCAGGTGGAGTGATACGAAGAGCTGATAGCCAGAGGCGCAGGTCCTGGATCCA
  AAAGGTTATTGAGCAAATAACAGGTTCCCCTAGACAGTGTGTCACCCTCTCGGAAGTGGTGCCTGTCACT
  GTACTGGCTGTCCAAAGATACCTTTTAGAGGATGAGCCACGGGATACAGTGCCCAAGCCTCCCCTTTACT
  GCTATGATGTGACAATCTCAGACGGGGTGTACCAGGAGAAGTGCTACTTAGACCCCAGCTTAAACTTTCT
  CGTTTATCAAAATATTCTTAAAGTTGGCATAGAGATGAGAATTTCCAGAGTCTCGTGCCTTTACAACGAG
  AAAAGATTGGGCCAGGGAATCCTGTGCATAGATAAGGTCCACTGTGGGGAGTCCCTAGATGTCATTTCTG
  TAGAAACTCCATTTAGAAACAGAGCGCATGAGGAAAAGCCAGAGAGGCCTTTAAGAGGGGGCAAGAGTCA
  CTACCTGGCTCTGTGGAATAACGAAGATCCGTACGGAGATATTTGGAAGACCAACAAGCAACCTGAGGAA
  TTCAATTTTAACAACATCAAAATAATCTCTCTCTCTCATCTTGAGTTGACTTGGAATAGCAGAAAGAATT
  TTCCTGCCTTGCTTGTAAGAGTCCTGCATAAATCAAAACTGCGATACTATGGAAAACCTAATAAAAAGAT
  GATTGAGCCATATCAGACCTATTTGGAGGTTGCTGACAGCTCGGGCATGGTGTCAGTGATTCTGTGGAAT
  GCTCTGTGTCCAGAGTGGTATAAAAGTTTGAGAGTGGGCTTGGTTCTTCTGCTTCAAGATTATACTGTGA
  AGAAGAGCTATCCATTAAGAATCCAGCCTGACCCTGCAGATCCACAGATGAAACTCATTTCTACTATGGA
  AATTTGCCTGAATCTTCGAGATCCACCAACTAACATAGTTATCATTCCAGAAAAGCAATTGAAATCAGAA
  TGGAAATTGCCAAAACTAATTAACCGATTCATCACAAGATCAGAACTAGACGATATGCCAGAAAAAAGCA
  TCTGTGATGTAATTGGTTTGTTATCCTTTGTAGGGAGGGTGCAACGGTCAAAGAAGAAAGAGAACAGTGA
  AGACTTTTGGTCATACCGCTGGATTCACATCACTGATGGGACTTCAGAGCAACCATTCATAGTGCAGCTG
  TTCTCCACATCGCAGCCAGAAGTCTTTGAAAATATTTACCCAATGACATATTTTGTGTGCACCCAGTTGA
  AAGTCGTTAGAAATAACAGCCAAGTACCTAAGCTGCTTTATCTTACCACTACAAATGAAAGCAGAGCACT
  TACTACTGGAGGGCATAGAGGCCTGCCATATACATACGATACCAAGGCAAAAAAAATTATTCAGTGGATT
  AAAACGAAGACTAATTTGGAAGCAAAGAACACGGTTATTGGTGGATATTACCCCTATCCACCAGTGCCAG
  AGACATTTTCCAAGTACAGTCGCTTTATTAAAGCTGAGTCACTCTTGACAACAATAAGTGAAGTAAAGAA
  GGTGATTGAAGACTTGCAGTATAGGGAACAAAAGCGCATTGCAATTCAGGGGATAATTACTGCTATAAAG
  TACATCCCCTATAAGCACTCAGCCGGAAGTGCCCCAGCACCAGAAGCATTCTGGAATGCTAGCCGTCCCT
  CAACATCTCAAGCAGCTGGGAAAGAAGATCATTGTCATGAACGAGGTAGTAAAAGGTCCCAAGATGACAG
  ACCAATGGGGTCTCAGCATTTTCAATATACCTCCAAGGTTTTGAGTCTCTGTGCAAAAAGAAAAATTCTT
  CAAGGCCCAAGTGCTAATCCGGTTCCTGTTCCTCAGCCGCATTCTTCTGCACAGATGAAAGGAAGCAAAC
  ACAACACACCAAGTCAAGAAAGTTCAACTGCTTATACCACAGGGAAGTCCAGAAGAATCACAAATGATCG
  ATGGGAGAGTCAGCTGTGGCAAGATAAGAAGTTTAGCTTGAGAGACCACCTACATTATGGTCACGTGGAT
  CCTGAAAGTATTCCACGGAAATTTATTTTGGAGCATGAAAAGTTTCTCACTCAGCAGTTTAATTCTCAGC
  CTGCAAAATACATACCACCAGAAGGAAAGCCCCCCAAACTTGATGAATTTCAGAGCGCCCGAAGCCTTGG
  ACATTTTGAAGTAACCATCCTAGGTTTGAACCATGAGATTGCGATCGATGTTGCATTTCTACCTATGTAT
  TCTCCAGAAGATGTTCAAGCATCGCAAATAGATACATTTTTGACTTGCATGAATTTCAGCTGTGTGTATC
  CACCAGCAGCACCTCTCAGTGGAAGGTTGCCAGATCCCAAGGCAGTTGCAGGTGATATTGTAAAGGCAGC
  AGCTGACCTGGATAGAGTCCACATCATTGGTATCTTGGATATCTGTAATTTGGGTAACAATAAAGTGGAA
  GTCTGTTTGCAGAAAATTTATACTCCAGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175326
• Insert Size: 2553 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175326.5, NP_780535.1
• RefSeq Size: 3738 bp
• RefSeq ORF: 2553 bp
• Locus ID: 102871
• UniProt ID: Q8C779
• Cytogenetics: X F1
• Gene Summary: Single-stranded DNA-binding protein recruited to replication forks to maintain genome stability. Prevents fork collapse by antagonizing the accumulation of RAD51 at forks to ensure the proper balance of fork remodeling and protection without interfering with the capacity of cells to complete homologous recombination of double-strand breaks.[UniProtKB/Swiss-Prot Function]