Nlgn1 (NM_138666) Mouse Untagged Clone

CAT#: MC222019

Nlgn1 (untagged) - Mouse neuroligin 1 (Nlgn1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nlgn1
• Synonyms: 6330415N05Rik; BB179718; mKIAA1070; NL1; Nlg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222019 representing NM_138666
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTTCCCAGATGCATGTGGCCAAATTATGTTTGGAGAGCTATGATGGCATGTGTGGTCCACAGGG
  GATCCGGTGCCCCATTGACTCTCTGCTTGTTGGGATGTTTGCTACAGACTTTTCACGTACTCTCTCAAAA
  GTTGGATGATGTAGACCCATTGGTTACTACTAACTTTGGCAAGATTAGGGGAATTAAGAAAGAACTCAAT
  AATGAAATTTTGGGTCCTGTCATTCAGTTTCTTGGGGTTCCATATGCCGCTCCACCAACAGGAGAACATC
  GTTTCCAGCCTCCAGAACCACCATCTCCCTGGTCTGACATCCGGAACGCCACTCAGTTTGCTCCTGTATG
  TCCCCAGAATATCATTGATGGCAGATTGCCTGAAGTTATGCTTCCTGTGTGGTTCACTAATAACTTGGAT
  GTGGTTTCATCATACGTCCAAGACCAGAGTGAAGACTGTCTATACTTAAACATCTATGTCCCAACTGAAG
  ATGGTCCCCTTACAAAGAAACACACAGATGATTTAGGTGATAATGACGGTGCTGAAGATGAAGATATTCG
  GGACAGTGGGGGTCCCAAACCAGTGATGGTGTACATCCATGGCGGCTCTTACATGGAAGGTACTGGAAAT
  CTGTATGATGGGAGTGTCTTGGCAAGCTATGGCAATGTGATCGTCATCACAGTCAACTATCGGCTTGGGG
  TACTTGGCTTCTTGAGCACAGGGGATCAGGCTGCCAAAGGAAACTACGGGCTCCTTGACCTCATCCAGGC
  CCTAAGATGGACCAGCGAGAACATTGGGTTCTTTGGTGGTGACCCCTTGCGAATCACTGTGTTTGGATCA
  GGCGCTGGGGGTTCATGTGTCAACCTGCTGACTTTATCCCATTATTCTGAAGGTAACCGTTGGAGCAATT
  CAACCAAAGGACTTTTTCAACGAGCAATAGCTCAGAGTGGAACAGCCCTTTCCAGCTGGGCTGTTAGTTT
  CCAGCCTGCAAAATACGCTAGAATTCTGGCCACAAAAGTTGGCTGCAATGTTTCAGATACAGTAGAGTTA
  GTAGAATGCCTGCAGAAGAAGCCTTACAAAGAACTTGTTGATCAAGATGTTCAACCAGCCCGATACCACA
  TAGCCTTTGGACCTGTGATCGATGGTGATGTAATACCAGATGACCCTCAGATACTGATGGAACAAGGAGA
  GTTCCTCAACTATGATATAATGTTGGGAGTTAACCAAGGGGAAGGGTTGAAGTTTGTCGAAAATATAGTA
  GATAGTGATGATGGTGTATCAGCCAGTGATTTCGACTTTGCTGTTTCTAATTTTGTTGATAATTTATATG
  GATATCCTGAAGGCAAAGATGTTTTGAGAGAAACCATTAAATTCATGTATACTGACTGGGCTGATCGCCA
  TAATCCTGAAACTAGAAGGAAGACATTGTTGGCTTTGTTTACGGACCATCAATGGGTAGCACCTGCTGTG
  GCCACAGCAGACCTTCACTCGAACTTTGGCTCACCTACATACTTCTATGCCTTTTATCATCATTGCCAAA
  CAGATCAAGTTCCAGCTTGGGCTGATGCAGCTCATGGGGATGAGGTTCCCTATGTGTTGGGAATCCCCAT
  GATTGGCCCTACAGAGTTATTTCCTTGCAATTTCTCCAAGAATGATGTGATGTTGAGTGCAGTAGTAATG
  ACATACTGGACGAATTTTGCTAAAACTGGTGACCCAAATCAACCAGTTCCTCAAGACACAAAATTCATCC
  ATACCAAACCCAACCGCTTTGAAGAAGTAGCATGGACCAGATATTCCCAGAAAGACCAGCTTTATCTCCA
  TATTGGATTAAAACCGAGAGTTAAAGAGCATTACAGAGCCAATAAGGTAAATCTCTGGCTGGAGCTGGTA
  CCTCATCTGCATAATCTCAATGACATTTCTCAGTATACCTCGACAACAACTAAAGTGCCATCCACGGACA
  TCACTCTCAGACCTACAAGGAAGAATTCCACACCAGTCACATCAGCCTTTCCCACTGCCAAACAGGATGA
  TCCCAAGCAACAACCAAGCCCCTTCTCGGTGGATCAGAGGGACTACTCCACAGAGCTAAGTGTCACTATC
  GCAGTGGGGGCCTCTCTGCTGTTTCTCAACATCTTGGCTTTTGCAGCCCTGTACTACAAGAAGGATAAGA
  GGAGACATGATGTCCACCGGAGGTGCAGCCCTCAGCGCACGACCACCAACGACCTAACCCATGCTCCAGA
  AGAGGAAATTATGTCTCTCCAAATGAAGCACACTGACTTGGATCACGAGTGTGAGTCCATCCATCCACAT
  GAGGTGGTTCTTCGGACCGCCTGTCCCCCAGATTATACTCTAGCTATGAGGAGGTCACCTGATGATATTC
  CACTAATGACACCTAACACCATCACAATGATTCCCAACACTATACCAGGGATTCAGCCCTTACATACATT
  CAACACATTTACTGGAGGACAGAATAATACACTGCCCCATCCCCACCCACACCCCCATTCACATTCAACA
  ACCAGGGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_138666
• Insert Size: 2532 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138666.3, NP_619607.2
• RefSeq Size: 5630 bp
• RefSeq ORF: 2532 bp
• Locus ID: 192167
• UniProt ID: Q99K10
• Cytogenetics: 3 A3
• Gene Summary: Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (PubMed:23716671).[UniProtKB/Swiss-Prot Function]