Afg3l2 (NM_027130) Mouse Untagged Clone

CAT#: MC221711

Afg3l2 (untagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Afg3l2
• Synonyms: 2310036I02Rik; AW260507; Emv66; par
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221711 representing NM_027130
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACCGCTGCCTGCTGCTGTGGAGCCGGGGCGGCTGCCGTCGCGGCCTTCCTCCCCTGCTCGTGC
  CCAGAGGTTGCCTGGGTCCGGACCGGCGGCCCTGCCTCCGTACGCTCTATCAATATGCTACTGTCCAGAC
  AGCAAGCAGCAGGCGTTCTCTGCTGAGGGATGTAATTGCTGCTTATCAAAGATTCTGTTCTCGACCTCCC
  AAAGGATTTGAAAAGTACTTTCCTAATGGGAAAAACGGAAAAAAGGCCAGTGAGCCTAAGGAGGCTGTTG
  GAGAAAAAAAAGAACCACAGCCCTCGGGCCCCCAGCCTTCTGGAGGTGCAGGTGGTGGGGGAGGGAAGCG
  CCGTGGCAAGAAAGAAGATTCTCACTGGTGGTCCAGGTTCCAGAAGGGTGACTTCCCATGGGATGACAAG
  GATTTCAGGATGTACTTTCTCTGGACTGCTCTTTTTTGGGGTGGAGTCATGATTTACTTCGTGTTCAAGA
  GCTCTGGGAGAGAAATCACGTGGAAAGACTTTGTCAATAACTATCTTTCTAAGGGCGTGGTGGACAGACT
  AGAAGTTGTCAACAAGCGTTTTGTTCGTGTGACCTTTACACCAGGAAAAACTCCGGTTGATGGGCAATAC
  GTCTGGTTTAATATTGGCAGTGTTGACACATTTGAGCGGAATCTGGAGACTTTGCAGCAAGAATTGGGCA
  TAGAAGGGGAGAACCGGGTCCCTGTGGTTTATATTGCTGAGAGCGATGGCTCCTTCCTGCTGAGCATGTT
  GCCCACCGTACTCATTATCGCTTTTCTACTCTACACCATAAGAAGAGGGCCCGCTGGCATTGGTCGGACC
  GGCCGGGGAATGGGTGGACTCTTCAGCGTTGGGGAAACCACAGCCAAGGTCTTAAAGGATGAGATAGATG
  TGAAGTTTAAAGATGTGGCTGGCTGTGAGGAGGCCAAGCTAGAAATAATGGAATTCGTGAATTTCTTGAA
  AAACCCAAAGCAATATCAAGACCTAGGAGCAAAAATCCCAAAGGGTGCCATTCTCACCGGTCCCCCAGGT
  ACTGGGAAGACGCTGCTAGCTAAGGCCACAGCTGGAGAAGCCAATGTCCCCTTTATCACTGTGAGCGGAT
  CTGAGTTTCTGGAGATGTTTGTTGGCGTTGGTCCAGCCAGAGTCCGAGACTTATTTGCCCTTGCTCGGAA
  GAATGCGCCTTGCATTCTCTTCATTGATGAGATTGATGCTGTGGGAAGGAAGCGCGGCAGAGGCAACTTC
  GGTGGGCAGAGCGAGCAGGAGAACACACTCAACCAGCTGCTTGTGGAGATGGACGGCTTCAACACAACCA
  CCAATGTGGTCATCCTGGCAGGCACAAATCGACCAGACATCCTGGATCCAGCTCTGTTGAGACCAGGCCG
  CTTTGACAGGCAGATTTTTATTGGACCCCCAGACATAAAAGGACGAGCCTCAATCTTCAAAGTTCACCTT
  CGACCATTGAAGCTGGACAGTGCCTTGGAAAAAGATAAATTGGCCAGAAAACTGGCGTCCTTAACTCCAG
  GGTTTTCAGGCGCTGATGTTGCCAATGTCTGCAATGAAGCTGCTTTGATTGCTGCAAGACACCTTTCAGA
  TGCCATTAATGAGAAGCACTTCGAACAAGCGATTGAGCGAGTGATTGGAGGCTTGGAGAAAAAAACCCAA
  GTTCTGCAGCCTGAGGAGAAGAAGACGGTGGCTTACCACGAAGCAGGCCATGCGGTCGCTGGCTGGTATC
  TGGAGCATGCAGACCCACTCTTAAAGGTCTCCATCATCCCGCGTGGCAAGGGGCTGGGCTATGCTCAGTA
  CTTGCCCAAGGAGCAGTATCTGTACACAAAGGAGCAGCTGCTGGACAGGATGTGCATGACTCTGGGCGGC
  CGTGTCTCCGAGGAGATCTTCTTTGGGAGAATTACAACCGGTGCCCAGGACGACTTGAGGAAGGTTACCC
  AGAGTGCCTATGCCCAGATCGTTCAGTTTGGCATGAACGAGAAAGTGGGGCAGATCTCCTTTGACCTCCC
  ACGACAGGGGGACATGGTGTTAGAGAAGCCTTACAGTGAGGCCACTGCGAGGATGATAGACGATGAAGTG
  AGGATACTCATCAGCGATGCCTACAGAAGGACGGTGGCTCTTCTCACAGAGAAGAAGGCTGACGTGGAGA
  AGGTCGCTCTCTTACTGTTAGAAAAGGAAGTCCTAGACAAGAATGACATGGTCCAGCTTCTCGGTCCCAG
  ACCATTTACAGAAAAGTCCACATATGAAGAATTTGTGGAAGGCACTGGCAGCTTAGACGAGGACACTTCT
  CTTCCTGAAGGCCTTCAGGATTGGAACAAGGAGCGGGAGAAGGAGGAGAAGAAGGAGAAGGAGAAGGAGG
  AGCCGCTGAATGAGAAGGTTGTCAGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027130
• Insert Size: 2409 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027130.1, NP_081406.1
• RefSeq Size: 3086 bp
• RefSeq ORF: 2409 bp
• Locus ID: 69597
• UniProt ID: Q8JZQ2
• Cytogenetics: 18 E1
• Gene Summary: ATP-dependent protease which is essential for axonal and neuron development (PubMed:18337413, PubMed:27642048). In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU (By similarity). Required for the maturation of paraplegin (SPG7) after its cleavage by mitochondrial-processing peptidase (MPP), converting it into a proteolytically active mature form.[UniProtKB/Swiss-Prot Function]