Vac14 (NM_146216) Mouse Untagged Clone

CAT#: MC221596

Vac14 (untagged) - Mouse Vac14 homolog (S. cerevisiae) (Vac14), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vac14
• Synonyms: AA959718; BC032215; D8Wsu151e; ingls; Tax1bp2; Trx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221596 representing NM_146216
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCAGAGAAGGATTTTGCGCCGCTCACTCCCAACATCGTAAGGGCCCTGAATGATAAACTCTACG
  AAAAGCGGAAGGTTGCAGCGCTGGAGATCGAGAAGCTGGTGCGGGACTTTGTGGCCCAGAACAACACCAT
  GCAAATCAAGCACGTGATCCAGACCCTGTCTCAGGAGTTTGCCCTGTCCCAGCACCCCCACAGCCGGAAA
  GGGGGCCTCATCGGTCTGGCAGCCTGCTCTATTGCCCTAGGCAAGGACTCAGGGCTCTACCTGAAGGAGC
  TGATAGAGCCAGTGCTGACCTGCTTTAATGACGCAGACAGCAGGCTGCGCTACTACGCTTGTGAGGCCCT
  CTACAATATCGTCAAAGTGGCCCGAGGTGCCGTGCTGCCGCACTTCAATGTTCTCTTCGATGGACTGAGT
  AAGTTGGCTGCTGACCCAGACCCCAATGTGAAAAGTGGATCTGAGCTCCTGGACCGCCTCTTAAAGGACA
  TTGTGACAGAAAGCAGCAAGTTTGACCTGGTGAGCTTCATCCCCCTTTTGCGGGAGAGGATTTACTCCAA
  CAACCAATACGCCCGACAGTTCATCATCTCCTGGATCCTGGTTCTGGTGTCGGTGCCAGACATTAACCTG
  CTGGATTACCTGCCAGAGATCCTAGATGGGCTCTTCCAGATCCTGGGTGACAATGGCAAGGAGATTCGGA
  AAATGTGTGAGGTGGTTCTTGGAGAGTTCTTGAAAGAAATTAAGAAGAACCCATCCAGTGTGAAGTTTGC
  TGAGATGGCCAACATCCTGGTGATCCACTGCCAGACCACAGATGACCTGATTCAGCTGACGGCTATGTGC
  TGGATGCGGGAATTCATCCAGCTGGCGGGCCGGGTAATGCTGCCCTATTCCTCTGGGATCCTGACTGCTG
  TCCTGCCATGCTTGGCCTATGATGACCGCAAGAAAAGTATCAAGGAGGTGGCCAATGTGTGTAACCAGAG
  CTTGATGAAGCTGGTCACCCCTGAGGATGATGAACCCGATGAGCCAAAGTCAGTAGCACAGAAGCAGACC
  GAACCCAACCCTGAGGATTCCCTGCCAAAGCAGGAGGGGACAGCCAGTGGAGGTCCAGGTTCCTGTGACT
  CCAGCTTTGGAAGTGGCATCAATGTCTTCACCTCAGCCAACACTGATAGGGCTCCAGTGACCCTGCACCT
  GGACGGGATTGTTCAAGTCCTGAACTGTCACCTTAGTGACACAACCATTGGGATGATGACCAGGATTGCT
  GTTCTGAAGTGGCTCTACCATCTCTACATCAAAACTCCTCGAAAGATGTTCCGGCACACAGACAGCCTCT
  TCCCCATCTTACTTCAGACATTATCTGACGAATCTGATGAGGTTGTCTTGAAGGATCTGGAAGTGTTGGC
  AGAAATTGCTTCCTCCCCTGCAGGCCAGACGGATGACCCAGGTGCACCTGATGGCCCTGACCTCCGAGTC
  AACCACTCGGAGCTTCAGGTGCCCACCTCCGGCAGAGCCAACCTACTAAACCCTCCCAGTACCAAAGGCT
  TAGAAGGCTCCCCCTCCACTCCCACCATGAACTCCTACTTTTACAAGTTCATGATCAACCTTCTACAGAC
  GTTCAGCAGTGAACGGAAGCTCCTGGAGGCCAGAGGCCCGTTCATCATCAGGCAGCTCTGCCTCCTGCTG
  AATGCAGAGAACATCTTCCATTCAATGGCAGACATCCTGCTTCGGGAGGAGGACCTCAAGTTCGCGTCCA
  CCATGGTGCACACCCTCAACACCATCCTGCTCACCTCCACCGAGCTCTTCCAGCTGAGGAACCAGCTCAA
  GGACCTGCAGACCCCGGAAAGCCAGAACCTCTTCTGCTGCCTGTACCGCTCCTGGTGCCACAACCCAGTC
  ACCACCGTGTCCCTCTGCTTCCTCACCCAGAACTACCGCCACGCCTATGACCTCATCCAGAAGTTTGGGG
  ACCTGGAAGTCACAGTGGATTTCCTCACAGAGGTGGACAAACTCGTGCAGCTAATTGAGTGCCCCATCTT
  TACATATCTACGCCTGCAACTGCTGGACGTGAAGAACAACCCATACCTGATCAAGGCCCTGTACGGCCTG
  CTCATGCTGTTGCCCCAGAGCAGTGCCTTCCAGCTGCTCTCTCACCGGCTCCAGTGTGTGCCCAACCCTG
  AGCTGCTGCAGACTGAAGACTGTCTGAAAGCGGCCCCCAAGTCCCAGAAAGGTGACTCCCCCAGCATCGA
  CTACACAGAGCTGCTGCAGCACTTTGAAAAAGTCCAGAAACAGCACCTGGAAGTAAGACACCAGCGCAGT
  GGGCGAGGGGATCACCTGGACCGCAGAGTTATCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146216
• Insert Size: 2349 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146216.2, NP_666328.2
• RefSeq Size: 3053 bp
• RefSeq ORF: 2349 bp
• Locus ID: 234729
• UniProt ID: Q80WQ2
• Cytogenetics: 8 57.59 cM
• Gene Summary: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.[UniProtKB/Swiss-Prot Function]