Brsk1 (NM_001003920) Mouse Untagged Clone

CAT#: MC221543

Brsk1 (untagged) - Mouse BR serine/threonine kinase 1 (Brsk1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brsk1
• Synonyms: Gm1100; SAD-B; SADB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221543 representing NM_001003920
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCGGGTCCAAGGAAGGTGGCGGGGGCTCCCCCGCCTACCACCTCCCACACCCACACCCACACC
  CACCCCAGCACGCCCAATATGTGGGCCCCTATCGCCTGGAGAAGACGCTGGGCAAAGGACAGACAGGTCT
  AGTTAAACTTGGAGTCCACTGCATCACGGGTCAGAAGGTCGCTGTCAAGATCGTGAACAGGGAGAAGCTG
  TCGGAATCGGTGCTGATGAAGGTGGAGAGGGAAATCGCCATCCTGAAGCTCATTGAACACCCGCACGTGC
  TCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTATACTTGGTTCTTGAGCACGTTTCTGGTGGTGA
  GCTGTTCGACTACCTGGTAAAAAAAGGGAGACTGACACCCAAGGAGGCCCGGAAGTTCTTCCGCCAGATC
  GTGTCAGCACTGGACTTCTGCCATAGCTACTCCATCTGTCACAGAGACTTGAAGCCAGAGAACCTGCTGT
  TGGATGAGAAAAACAACATCCGCATCGCAGACTTTGGTATGGCGTCCCTGCAAGTGGGGGACAGCCTCCT
  GGAGACCAGCTGCGGGTCCCCCCATTACGCATGTCCAGAGGTGATCAAGGGGGAAAAGTATGATGGCCGC
  CGGGCAGACATGTGGAGCTGTGGAGTCATCCTATTTGCCCTGCTTGTGGGGGCACTGCCCTTCGATGACG
  ACAACCTGCGCCAGCTACTGGAGAAGGTGAAACGTGGGGTCTTCCACATGCCTCACTTCATCCCTCCAGA
  CTGCCAGAGCCTCCTGAGAGGGATGATTGAAGTGGAGCCCGAGAAAAGGCTCAGTCTGGAGCAAATTCAG
  AAACATCCTTGGTATCTGGGCGGGAAACACGAACCAGACCCTTGCCTGGAGCCAGCCCCAGGCCGCAGAG
  TAGCCATGCGTAGCCTGCCTTCCAATGGCGAGCTGGACCCTGACGTTCTGGAAAGCATGGCGTCTCTGGG
  CTGCTTCAGAGACCGCGAGCGGCTGCACAGAGAACTGCGAAGCGAGGAGGAAAACCAAGAAAAGATGATC
  TATTATTTGCTTTTGGATCGGAAAGAGCGGTATCCTAGCTGTGAAGACCAGGACCTGCCTCCTCGGAATG
  ATGTTGACCCACCTCGTAAGCGTGTGGATTCCCCCATGCTGAGCAGACACGGGAAACGGAGGCCAGAGCG
  GAAGTCCATGGAAGTCCTCAGTATCACAGATGCTGGGAGTGGTGGCTCCCCAGTGCCTACCAGACGGGCC
  TTGGAGATGGCCCAGCACAGTCAGAGATCCCGCAGCGTCAGTGGAGCCTCCACTGGTCTGTCCTCCAGCC
  CTCTGAGCAGCCCAAGGAGTCCGGTCTTTTCCTTCTCACCGGAGCCAGGCGCTGGAGATGAGGCTAGAGG
  CGGAGGCTCCCCAACTTCCAAAACACAGACGCTGCCTTCTCGGGGCCCCAGAGGTGGGGGCGCTGGAGAG
  CAGCCACCACCCCCCAGTGCCCGTTCCACGCCCTTGCCTGGACCACCAGGCTCCCCGCGCTCCTCCGGCG
  GAACCCCCTTGCACTCACCTCTGCACACGCCTCGAGCCAGCCCTACGGGGACCCCAGGAACGACGCCACC
  TCCCAGCCCAGGCGGTGGCGTCGGGGGAGCCGCCTGGAGAAGTCGGCTCAACTCCATCCGCAACAGCTTC
  CTGGGCTCCCCTCGCTTCCACCGGCGCAAGATGCAGGTCCCTACCGCCGAGGAGATGTCCAGTTTGACAC
  CAGAATCCTCTCCTGAGCTGGCAAAACGCTCCTGGTTCGGGAATTTCATCTCCTTGGACAAAGAAGAACA
  AATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCGGACATTGTCCATGCCTTTCTGTCG
  ATCCCCAGCCTGAGTCACAGTGTACTGTCACAGACCAGCTTCAGGGCCGAATACAAGGCCAGCGGCGGCC
  CCTCCGTCTTCCAGAAGCCTGTCCGCTTTCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAACCCTCACC
  CCGAAGAGATGGCAGCAGTGGAGGTGGCATCTACTCAGTCACCTTTACTCTCATCTCCGGTCCCAGCCGT
  CGGTTCAAACGTGTGGTAGAGACCATCCAGGCACAGTTGCTGAGTACTCACGACCAGCCCTCCGTGCAGG
  CCTTGGCAGATGAGAAGAACGGAGCCCAGACCCGGCCTGCTGGGACCCCACCCCGAAGCCTGCAGCCCCC
  ACCAGGCCGCTCTGACCCTGATCTGAGTAGCTCTCCCCGCCGAGGACCCCCTAAGGACAAGAAGCTCCTG
  GCCACCAATGGAACCCCTCTACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003920
• Insert Size: 2337 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003920.3, NP_001003920.2
• RefSeq Size: 3014 bp
• RefSeq ORF: 2337 bp
• Locus ID: 381979
• UniProt ID: Q5RJI5
• Cytogenetics: 7 A1
• Gene Summary: Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).