Irf2bpl (NM_145836) Mouse Untagged Clone

CAT#: MC221530

Irf2bpl (untagged) - Mouse RIKEN cDNA 6430527G18 gene (6430527G18Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Irf2bpl
• Synonyms: 6430527G18Rik; Eap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221530 representing NM_145836
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGGCGCAGGTGTCCTCGTCCCGAAGACAGTCTTGCTACCTGTGCGACCTGCCTCGCATGCCCT
  GGGCCATGATCTGGGACTTCTCCGAACCCGTATGCCGTGGTTGCGTCAATTACGAAGGCGCCGACCGCAT
  AGAATTCGTGATCGAGACAGCACGCCAGCTGAAGCGGGCTCACGGTTGCTTCCAAGACGGTCGCTCCCCG
  GGGCCGCCGCCGCCAGTCGGGGTCAAGACGGTGGCCTTGTCCGCCAAGGAAGCGGCAGCAGCAGCTGCCG
  CCGCACAGCAACAGCAGCAGCAACAACAGCAGCAACAGCAGCAGCTCAACCACGTCGATGGTTCCACCAA
  ACCTGCAGTGTTGGCCGCCCCGTCCGGTCTGGAGCGTTATGGCCTGAGCGCAGCGGCCGCCGCGGCTGCC
  GCCGCCGCTGCGGTGGAACAACGCAGCCGGTTCGAGTATCCGCCTCCTCCGGTGAGTCTGGGGAGCAGCA
  GCCACGCCGCCCGGCTGCCCAACGGCCTGGGAGGTCCTAACGGCTTCCCCAAGCCGGCACCAGAGGAAGG
  CCCCCCGGAGCTGAACCGCCAGAGCCCCAACTCATCCTCAGCAGCAACATCGGTGGCTTCTCGGCGTGGG
  ACGCATAGTGGGTTGGTGACCGGGCTGCCCAACCCGGGTGGTGGCGGGGGACCTCAGCTAACCGTGCCCC
  CCAACCTATTGCCGCAGACGCTGCTTAACGGCCCGGCCAGCGCCGCGGTGCTGCCTCCGCCCCACGGGTT
  GGGGGGAAGCCGAGGGCCCCCGACTCCAGCACCTCCGGGTGCTCCCGGGGGCCCCGCATGTCTCGGGGGT
  CCCCCGGGTGTGTCAGCCACTGTATCCTCCGCTCCATCATCGACTTCGTCGACGGTGGCAGAGGTGGGCG
  TGGGTGCTGCTGGCAAGAGGCCTGGATCGGTGTCGAGCACGGACCAGGAGCGTGAGCTAAAGGAGAAGCA
  GCGCAACGCTGAGGCCTTGGCAGAGCTGAGCGAGAGCCTGCGCAACCGCGCGGAGGAGTGGGCCAACAAG
  CCCAAGATGGTCCGCGACACGCTGCTCACGCTGGCTGGCTGCACGCCCTACGAGGTGCGCTTCAAGAAGG
  ACCACTCGCTTTTGGGTCGCGTCTTTGCCTTCGACGCGGTCTCTAAGCCGGGCATGGACTATGAGCTGAA
  GCTGTTCATTGAGTATCCCACGGGCTCCGGCAATGTGTACTCCAGCGCGTCTGGGGTGGCCAAACAGATG
  TACCAGGATTGCATGAAGGACTTTGGCCGGGGTCTGTCCTCTGGCTTCAAGTACCTGGAGTACGAGAAGA
  AGCACGGCTCTGGCGACTGGCGCCTACTTGGGGACCTACTGCCTGAGGCTGTGCGCTTCTTCAAGGAGGG
  CGTGCCCGGCGCGGACATGCTGCCCCAGCCCTACCTAGATGCTAGCTGTCCCATGCTACCCACGGCGCTG
  GTGAGTCTCAGCCGCGCCCCCAGCGCACCTCCAGGAACTGGAGCCTTGCCACCCGCGGCGCCCACGGGCC
  GGGGTGCAGCGTCCAGCCTGCGCAAAAGGAAGGCATCCCCGGAGCCTCCAGACTCAGCTGAAAGCGCACT
  GAAGCTCGGTGAGGAACAGCAGAGGCAGCAGTGGATGGCAAACCAGAGCGAGGCGCTGAAGCTCACGATG
  TCCGCGGGGGGCTTTGCGGCGCCGGGACACTCAGCAGGGGGACCACCTCCGCCCCCTCCACCTCTGGGAC
  CTCATTCCAATCGGACCACCCCGCCGGAGTCAGCCCCCCAGAACGGTCCGTCCCCCATGGCTGCCCTCAT
  GTCGGTAGCAGACACTTTGGGCACAGCTCATTCGCCCAAGGACGGCAGTTCAGTGCACTCGACCACTGCA
  TCTGCTAGGCGAAACAGCAGCAGCCCGGTGTCACCCGCCTCCGTGCCGGGCCAGCGTCGCCTGGCATCAC
  GCAATGGGGACCTGAATTTACAGGTGGCGCCCCCGCCGCCTAGCGCCCACCCGGGCATGGACCAAGTACA
  CCCCCAAAACATTCCGGATTCCCCTATGGCCAACAGCGGACCCCTCTGCTGTACCATTTGCCACGAACGT
  TTGGAGGACACGCATTTCGTTCAGTGTCCGTCTGTCCCTAGCCACAAATTTTGTTTCCCTTGTTCTAGAG
  AGAGTATCAAGGCCCAGGGGGCCACAGGTGAGGTGTATTGCCCCAGCGGAGAGAAATGCCCCCTAGTCGG
  GTCTAATGTACCTTGGGCCTTCATGCAGGGCGAAATTGCAACTATCTTAGCTGGGGATGTTAAAGTGAAA
  AAAGAGAGAGACCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145836
• Insert Size: 2328 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145836.2, NP_665835.1
• RefSeq Size: 4112 bp
• RefSeq ORF: 2328 bp
• Locus ID: 238330
• UniProt ID: Q8K3X4
• Cytogenetics: 12 D2
• Gene Summary: Probable E3 ubiquitin protein ligase involved in the proteasome-mediated ubiquitin-dependent degradation of target proteins. Through the degradation of CTNNB1, functions downstream of FOXF2 to negatively regulate the Wnt signaling pathway. Probably plays a role in the development of the central nervous system and in neuronal maintenance (By similarity). Also acts as a transcriptional regulator of genes controlling female reproductive function. May play a role in gene transcription by transactivating GNRH1 promoter and repressing PENK promoter (By similarity).[UniProtKB/Swiss-Prot Function]