Hectd2 (NM_172637) Mouse Untagged Clone

CAT#: MC221524

Hectd2 (untagged) - Mouse HECT domain containing 2 (Hectd2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hectd2
• Synonyms: 4921524L07; A630025O09Rik; AW212605
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221524 representing NM_172637
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGGCGGCTCGGGATCTCTCGCCCGGAGCGCCGCCGGCGGTAGCCGCGGCCGCCCCGGAGGAGA
  GGAAGGGGAAAGAACCGGAGCGCGAGAAGCTGCCGCCCATCGTGACGGCCGGCGCAGCCGCGGGCTTGGA
  CAGAGGATCCAAGGGCCAGATTTCCACCTTCAGCAGCTTTGTCTCGACTGTCACCCAGAAGAAGGAAGCT
  GCTGAGAACAGAAGCTCGCCTACCCACCTTGCTCTCCCTAACATCAGGAATGTGAGAGACCTACCACCAA
  TTTGTCTTGATGTTAGACAAAAACAGCGCATGTCTGTGGAAGCGTTACCATCTGAAGTGAAGGTCCCGCC
  CCTTCCAGAACCTTCTCTTCCTAGCCAGCCCAAAACTGTAAAAGACTTTGAGGAAGATTTAGAAAAAGCT
  GAGGCCACAGGGAATTGGAAGACAGTACATGCTTTTTATATAACAGCATTTGATTCTTTCACAGAACTAA
  ACACTGCATTTAAGAAAGATGCCACGGCCTCATTTAATACTATTGAGGACTCTGGGCTTAATGCCAATTT
  GGTGAATGCTGTGTTTGATGCCTTACTTAATACTCCTCAAGATATCCAGAAGTCAGTATTGAAGGGAATC
  ATTAACAGCTTGTTACAAGAATGGAAAGGTCCACGAACAAAAGATGATCTTAGAGCATATTTTATACTGT
  TACAGAATCCTCAATTTAATATCACATCTACATATGTCATCTATGCTCATTTGCTACGACAGATAGCTAC
  CTTAGTGGAAGCTGACCATCACTTCCTAGTTCACTGGCTTAAAAAATTATCCCAGAAGAAATTCAAGCAA
  CTGGTAGAGAGATTGCTGCAATTTGTTTCTTTACGGCTGTTTCCAGCAAAACCTGAAGAATTCCCGCCTC
  TAACGAAGTGTACCTGGTGGATCCCATCAGCCGCTAAAGTTCTGGCTTTGCTTAACACTGCCAACAATTT
  GGTTCACCCTCCCCTCGTTCCTTACACTGATTTCTATAATTCTACCCTGGATCACATTGATCTCATGGAA
  GAGTATCACACGTGGCAGAGCTTTGGGAACTCTCACAGGTTTTCCTTCTGTCAGTACCCATTCGTTATTT
  CTATAGCTGCAAAAAAAATCATTATTCAAAGAGACTCAGAACAACAGATGATAAGCATCGCAAGGCAAAG
  TCTGGTGGATAAAGTATCTCGAAGACAAAGACCTGATATGAATATGTTATTTCTAAATATGAAAGTAAGG
  AGGACACATCTGGTTAGCGACTCACTTGATGAGTTAACCCGGAAGAGAGCTGACTTGAAGAAGAAGTTGA
  AAGTTACATTTGTAGGTGAAGCTGGTTTGGATATGGGCGGCTTGACTAAAGAATGGTTCCTTCTTCTAAT
  TCGCCAGATTTTCCATCCAGATTATGGCATGTTTACATACCACAAGGATTCACACTGCCATTGGTTTAGC
  AGCTTTAAATGTGATAACTATTCTGAATTCCGATTGGTTGGAATTCTCATGGGACTAGCTGTTTATAACA
  GCATCACCTTGGATATTCGTTTTCCTCCCTGCTGTTACAAAAAATTACTGAGCCCTCCTGTCGTACCAAG
  TGATCAGAGTACACCAGTAGGCATCTGCAGTGTTACCATTGACGACTTGTGCCAGGTTATGCCTGAATTG
  GCCCATGGACTAAAGGAACTTTTATCATATGAAGGCAACGTTGAAGAAGATTTCTATTCAACATTTCAGG
  TTTTTCAAGAAGAATTTGGAGTAATTAAATCCTATAACTTAAAACCAGGGGGTGATAAGATTCCAGTTAC
  CAATCAGAATAGAAGAGAGTATGTACAGCTTTATACTGACTTCCTGTTGAACAAATCTATCTATAAGCAA
  TTTGCTGCATTTTACTGTGGATTTCATAGTGTGTGTGCTTCAAATGCCCTAATGCTGCTTCGTCCAGAAG
  AAGTAGAAATCCTGGTTTGTGGCAGTCCTGAACTGGATATGCATGCACTGCAGAGGAGCACACAGTACGA
  CGGCTATGCGAAGACAGACCTGACCATACGATACTTTTGGGATGTTGTGCTTGGATTTCCACTTGAACTT
  CAGAAAAAGCTGTTACACTTTACTACAGGAAGCGACAGAGTACCTGTTGGAGGGATGGCTGATTTGAATT
  TTAAAATTTCAAAGAATGAAACTTCTACTAACTGGTTACCTGTGGCACACACTTGTTTCAATCAACTCTG
  CCTTCCCCCATACAAGAGCAAAAAAGATCTGAAACAGAAACTGATTATTGGAATTTCAAATTCAGAAGGT
  TTTGGACTTGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172637
• Insert Size: 2325 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172637.3, NP_766225.2
• RefSeq Size: 4771 bp
• RefSeq ORF: 2325 bp
• Locus ID: 226098
• UniProt ID: Q8CDU6
• Cytogenetics: 19 C2
• Gene Summary: Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the middle portion of the coding region, compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1.