Fbxo30 (NM_001168297) Mouse Untagged Clone

CAT#: MC221308

Fbxo30 (untagged) - Mouse F-box protein 30 (Fbxo30), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxo30
• Synonyms: 1700026A16Rik; MUSA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221308 representing NM_001168297
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAGAGGTACAGCAGCACTCACACTGTATGAACTGTGTCAGTAGACGGTGTATGACCAGACCAG
  AGCCTGGGGTTTCCTGTGACTTGATTGGCTGTCCATTGGTTTGTGGAGCAGTTTTCCATTCTTGTAAAGC
  TGACGAACATCGGCTCTTGTGTCCCTTTGAACGAGTAGCTTGCTTAAATAGAAACTTTGGATGTCCATTT
  ACCCTGGCACGAAATAAAGTTGCTGAACATCTAGAAATGTGTCCTGCAAGTGTGGTGTGCTGTACTATGG
  AATGGAATCGATGGCCCGTTAGTTATTCAGACCGGAAGTCATATGAAAGTCTAAGCAGAGATGTTGATGA
  AGTGGCACAATTAGACATGGCTTTGGCTCTTCAAGATCAAAGAATGCTTTTGGAATCTCTTAAAGTAGCC
  ACCATGATGTCAAAAGCAACTGATAAAATATCTGAACCTAGAGAACAAATCTCAGTGAAATCAAGTGTTC
  AGGAAATACCACGTACTAACGGGTTGGTATCCGTTGATGAAGAATCCTATGGTGCACTTTATCAAGCTAC
  TGTAGAAACAACCAGAAGTTTGGCTGCTGCTTTAGATATCCTAAATTCTGCCACAAGAGACATTGGCATG
  TTAAATACAAGTCTCCATGCTACTGCGAATGAAATGGATGAGGAGAATAACAAAGAAAGCTTCCAGGACA
  AAAACTTGAAAGACCAGGACCACCTTGATGAGGGTGAGATAGGGGCAGTAGGTGGAGTTGATTATAGTGG
  CACAAGTCAGAATGCTCAGGCTGAACAAAATGGCTCAAGTGATTTGTTATGTGACTTAAATCCAAGTTCT
  AATGGTACTTCTGCTCTTTGCAATGGCTTTCCTTTGGAAAAGATGTGTATTCAGGTCAAAGGCCAGGATC
  AAAATTTTCATGGTGATTCCACAGAAAGTAATATAACAAATGGAGATTGTGTGGAAGCTGATGGTACTTC
  AGAACCTTCCAGTTCACTTGTAGTACCAGAACAACTGAGGGAAATAAGTCCTTTCAGTGCTTTGCCAGAT
  AGTACATTTCAGCAAATCCTCATGCCAGATGAAGACGATGAGAAGGACTTGTGTTGGAAAAAAGTAGACT
  TGGGAGACTTGAAGGATGTTAATGGTTCACCTTTCAGTCATGCGCCTTCATTCAAGTTTCTTTCTAATTC
  ATGGTATATACCAAAGGAAGACAAAGCAGTTGATACATCAGATTTAGAAGTTGCAGAAGATCCCATGGGC
  CTCCAAGGAATAGATCTGATTACAGCAGCATTACTGTTTTGTCTGGGAGATTCTCCAGGTGGGAGAGGTA
  TATCTGATAGCCGCATGACTGATGTTTATCACGTTGACTTTGGGACACAGACTTTTTCACTTCCATCTGC
  AATATTAGCTACAAATACAATGGTTGGAGAAATAGCTTCGGCTTCAGCTTGTGATCATGCCAATCCACAG
  CTTTCAAATCCAAGCCCTTTTCAGACACTTGGGCTGGACTTAGTTTTGGAATGTGTTGCTAGGTACCAGC
  CTAAGCAGCGTTCAATGTTTACCTTTGTATGCGGACAGCTATTTAGAAGAAAAGAATTTTCTTCCCATTT
  TAAGAATGTGCATGGTGACATTCATGCTGGACTCAATGGATGGATGGAACAGAGGTGTCCTTTAGCTTAT
  TATGGTTGTACCTATTCTCAGCGTAGATTTTGCCCATCGACACAAGGAGCAAAGATCATACATGATCGCC
  ATTTAAGGTCATTTGGAGTTCAGCCATGTGTATCTACAGTATTAGAAGAACCTTCTAGAAACTGTGTGTT
  GGGATTGCGTAGTGACCATCTAAGTAGTCTCCCTTTTGAGGTACTCCAACATATTGCAGGCTTCCTTGAC
  GGCTTCAGTTTATGCCAGCTTGCATGTGTATCCCGGCTAATGAGGGACGTTTGTGGCAGTTTACTTCAGT
  CTCGTGGAATGGTAATCTTGCAGTGGGGGAAGAAGAAGTATCCGGAAGGAAATTCATCATGGCAGATAAA
  AGAGAAGGTATGGCGATTCAGTACTGCATTTTGTTCTGTTAATGACTGGAAATTTGCTGACATCCTGAGC
  ATGGCTGACCACTTGAAGAACTGCAGTTACAACGTGATAGAGAAACGGGAGGAAGCCATCCCATTGCCGT
  GTATGTGTGTGACTCGAGAGCTTACAAAAGAAGGACGTTCCCTGCGCTCAGTTTTAAAGCCTGTGCTTTA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001168297
• Insert Size: 2241 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168297.1, NP_001161769.1
• RefSeq Size: 4943 bp
• RefSeq ORF: 2241 bp
• Locus ID: 71865
• UniProt ID: Q8BJL1
• Cytogenetics: 10 A1
• Gene Summary: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.