Nrros (NM_146069) Mouse Untagged Clone

CAT#: MC221126

Nrros (untagged) - Mouse leucine rich repeat containing 33 (Lrrc33), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nrros
• Synonyms: E430025L02Rik; Lrcc33; Lrrc33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221126 representing NM_146069
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGAGCCTTTGGAGACTGGAAGCATTGAATCCTCTGGAACTGGCAATGTTGTTGTGAGCCACCAGA
  GAGCTGTGCCTGAGATGGAGTTCCCGCCCCTCTGGCTTTGCCTGGGTTTTCACTTCTTGATTGTGGAATG
  GAGGAGCGGACCTGGGACAGCCACTGCAGCTTCCCAAGGAGGCTGCAAGGTGGTCGACGGAGTCGCTGAC
  TGCCGGGGTCTGAACCTTGCTTCGGTACCCAGCAGCCTCCCACCCCATTCCCGGATGCTCATTCTGGATG
  CTAACCCTCTCAAGGACCTGTGGAACCATTCCCTCCAGGCCTACCCACGCCTGGAGAACCTCAGCCTGCA
  TAGCTGTCACCTGGACCGTATCAGCCACTACGCCTTCCGAGAGCAAGGCCACTTACGCAACCTGGTTCTG
  GCAGACAACCGCCTCTCGGAGAACTACAAGGAGTCAGCCGCAGCACTCCACACCCTGCTGGGACTTCGGA
  GACTGGACTTGTCTGGAAACTCCCTGACCGAAGACATGGCGGCTCTCATGCTTCAGAACCTCTCCTCGTT
  GGAGGTTGTGTCCTTGGCAAGGAATACCCTCATGAGGCTCGACGACTCTATCTTTGAGGGCCTGGAACAC
  CTTGTAGAGCTAGATTTGCAGAGAAACTACATCTTTGAGATCGAGGGCGGGGCGTTTGACGGCTTGACTG
  AGCTGAGGCGTCTCAACCTTGCCTATAACAACCTCCCCTGCATTGTGGACTTCAGCCTCACCCAGTTACG
  GTTCCTCAACGTCAGTTATAACATCCTAGAGTGGTTCCTGGCAGCCAGGGAGGAGGTGGCCTTCGAATTG
  GAGATACTGGACCTGTCTCACAACCAGCTGCTCTTCTTCCCACTCTTGCCTCAGTGCGGCAAGCTGCACA
  CCCTCCTGCTCCAGGACAACAACATGGGCTTCTACAGGGAGCTTTACAACACCTCCTCCCCGCAGGAGAT
  GGTGGCCCAGTTCCTTCTTGTGGACGGCAATGTGACTAACATCACAACTGTCAACCTCTGGGAGGAGTTT
  TCCTCCAGCGACCTGTCAGCTCTTCGCTTCCTGGACATGAGCCAAAACCAGTTCCGGCACTTGCCGGATG
  GTTTCCTGAAGAAGACACCTTCCCTTTCCCACCTGAACCTCAACCAGAACTGCCTGAAGATGCTCCACAT
  CCGGGAGCACGAGCCGCCGGGAGCACTCACCGAGCTGGATCTGAGCCACAACCAGTTGGCAGAGCTGCAC
  CTGGCTCCGGGGCTCACGGGCTCCCTCAGGAACCTCCGGGTGTTCAACCTGAGTTCCAACCAGCTCCTGG
  GGGTCCCCACTGGCCTTTTCGACAATGCCAGCAGCATCACTACAATCGACATGAGTCACAATCAGATCTC
  ACTTTGTCCTCAGATGGTGCCCGTAGACTGGGAGGGGCCTCCCAGTTGTGTGGATTTCAGAAACATGGGC
  TCTTTGAGGAGCCTTTCTCTGGACGGCTGTGGGCTGAAGGCATTACAAGACTGCCCGTTCCAGGGGACCT
  CCCTCACTCATTTAGACCTGTCTAGCAACTGGGGGGTTCTGAATGGGAGCATCAGCCCTCTCTGGGCTGT
  TGCCCCGACATTACAGGTCCTGTCTCTCAGGGACGTGGGCCTTGGTTCTGGCGCTGCAGAGATGGACTTC
  TCTGCGTTTGGGAATCTGCGGGCGTTGGATCTGTCGGGAAACTCCCTGACCAGCTTCCCAAAGTTCAAGG
  GCAGTTTGGCCCTTCGGACTCTCGACCTCCGCAGAAACTCTCTCACGGCCCTCCCTCAGAGGGTTGTGTC
  CGAGCAGCCTCTGAGGGGTCTGCAGACCATCTACCTCAGCCAGAACCCTTATGACTGCTGTGGGGTGGAA
  GGATGGGGGGCCCTGCAGCAGCACTTCAAGACTGTTGCGGACTTGTCCATGGTCACTTGCAACCTCTCTT
  CCAAGATCGTCCGTGTGGTGGAGCTGCCCGAAGGCCTGCCTCAGGGCTGTAAGTGGGAACAGGTGGACAC
  TGGTCTCTTCTACCTCGTGCTCATCCTGCCCAGCTGCCTCACCCTGCTGGTGGCCTGTACTGTCGTCTTC
  CTCACTTTTAAGAAGCCTTTGCTTCAGGTCATCAAGAGCCGCTGCCACTGGTCCTCCATATACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146069
• Insert Size: 2166 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146069.4, NP_666181.2
• RefSeq Size: 3559 bp
• RefSeq ORF: 2082 bp
• Locus ID: 224109
• UniProt ID: Q8BMT4
• Cytogenetics: 16 B2
• Gene Summary: Key regulator of transforming growth factor beta-1 (TGFB1) specifically required for microglia function in the nervous system (PubMed:29909984). Required for activation of latent TGF-beta-1 in macrophages and microglia: associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGFB1, and regulates integrin-dependent activation of TGF-beta-1 (PubMed:29909984). TGF-beta-1 activation mediated by LRRC33/NRROS is highly localized: there is little spreading of TGF-beta-1 activated from one microglial cell to neighboring microglia, suggesting the existence of localized and selective activation of TGF-beta-1 by LRRC33/NRROS (PubMed:29909984). Indirectly plays a role in Toll-like receptor (TLR) signaling: ability to inhibit TLR-mediated NF-kappa-B activation and cytokine production is probably a consequence of its role in TGF-beta-1 signaling (Probable).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (2). ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.