Hook3 (NM_207659) Mouse Untagged Clone

CAT#: MC221058

Hook3 (untagged) - Mouse hook homolog 3 (Drosophila) (Hook3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hook3
• Synonyms: 5830454D03Rik; AI317159; E330005F07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221058 representing NM_207659
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACGTGGAGTCTGTGGAGCGGGTGGAGCTGTGCGAGAGCCTGCTCACTTGGATCCAGACCTTTA
  ATGTGGATGCACCGTGCCAGACCGCGGAGGACTTAACGAACGGCGTTGTCATGTCCCAGGTTCTTCAGAA
  GATAGATCCTGTATATTTCGACGATAATTGGCTAAACAGAATCAAAACTGAAGTAGGAGACAATTGGAGG
  TTAAAGATCAGCAATTTAAAGAAAATTTTAAAAGGCATCTTGGATTACAATCATGAGATTTTAGGACAGC
  AGATTAATGATTTCACCCTTCCTGACGTGAACCTCATTGGGGAGCATTCGGATGCAGCAGAGCTCGGAAG
  GATGCTGCAGCTCATTCTAGGCTGTGCTGTGAACTGTGAGCAGAAGCAAGAGTACATTCAAGCCATTATG
  ATGATGGAGGAATCTGTTCAGCATGTCGTCATGACAGCCATTCAAGAGCTAATGAGTAAAGAGTCTCCTG
  TCTCTGCTGGACATGATGCCTATGTTGATCTTGATCGCCAGTTGAAGAAAACTACCGAAGAACTAAATGA
  AGCTTTGTCAGCAAAGGAGGAAATTGCTCAAAGATGCCATGAATTGGATATGCAGGTTGCAGCACTGCAA
  GAGGAGAAAAGTAGTTTATTGGCAGAAAATCAGATACTAATGGAACGACTCAATCAATCTGATTCTATAG
  AAGATCCCAATAGCCCAGCAGGAAGAAGACATTTGCAACTTCAGACTCAGTTAGAACAGCTGCAAGAAGA
  AACATTCAGACTAGAAGCAGCCAAAGACGATTATCGAATCCGCTGTGAGGAGTTAGAAAAGGAAATCTCT
  GAGCTTCGGCAGCAGAACGATGAGCTGACCACTTTGGCAGACGAAGCTCAGTCTCTAAAGGATGAAATAG
  ACGTTCTCAGACACTCTTCTGATAAAGTATCTAAACTAGAAGGCCAAGTCGAATCTTATAAGAAGAAGCT
  GGAAGACCTTGGAGATTTGAGGCGGCAGGTTAAGCTCTTGGAAGAAAAGAATACGATGTACATGCAGAAC
  ACTGTCAGTCTGGAGGAAGAGTTAAGAAAAGCTAATGCCGCCCGGGGACAGCTTGAGACATACAAGAGAC
  AAGTTGTAGAGCTGCAAAATAGATTATCTGACGAATCAAAGAAAGCAGATAAGTTAGATTTTGAATATAA
  GCGGCTAAAAGAAAAAGTTGATGGTCTTCAGAAAGAAAAAGATAGGTTAAGAACGGAAAGAGATTCTCTG
  AAGGAAACCATTGAAGAGTTGCGTTGTGTGCAGGCTCAGGAGGGGCAGCTCACCACTCAAGGGTTAATGC
  CTCTGGGAAGCCAGGAATCTTCAGACAGCCTTGCAGCAGAGATTGTTACACCCGAAATCAGGGAGAAACT
  TATTCGTCTTCAGCATGAGAATAAAATGTTAAAACTCAATCAAGAGGATTCAGACAATGAGAAAATTGCC
  TTATTGCAGAGCCTTCTAGATGATGCAAATCTACGCAAGAATGAGCTGGAGACGGAGAACAGGCTTGTGA
  ATCAGAGACTTCTAGAAGTACAGTCACAAGTAGAAGAACTGCAAAAATCTTTGCAGGATCAAGGTTCAAA
  AGCAGAAGATTCAGTTCTTCTAAAAAAGAAGCTTGAAGAACATCTAGAGAAGCTGCATGAAGCCAACAAT
  GAATTGCAGAAAAAGAGAGCCATTATTGAAGATCTCGAGCCAAGATTTAACAACAGCTCCTTAAGAATTG
  AAGAATTGCAAGAGGCTTTACGGAAGAAAGAAGAAGAAATGAAGCAAATGGAAGAACGCTATAAAAAATA
  CTTAGAGAAGGCCAAGAGTGTTATCCGTACATTAGACCCTAAACAGAATCAAGGAGCAGCACCAGAAATT
  CAAGCTCTTAAAAATCAACTGCAGGAACGGGACCGACTCTTCCACTCATTAGAGAAAGAATATGAGAAAA
  CGAAGAGTCAAAGAGACATGGAGGAGAAATACATTGTTAGTGCCTGGTACAATATGGGAATGACTCTACA
  TAAAAAGGCGGCTGAAGACAGACTAGCTAGCACAGGTTCAGGGCAGTCATTTCTGGCTAGGCAAAGACAA
  GCAACCAGCACAAGAAGATCCTACCCAGGCCATGTCCAGCCAGCCACAGCAAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_207659
• Insert Size: 2157 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207659.3, NP_997542.1
• RefSeq Size: 12836 bp
• RefSeq ORF: 2157 bp
• Locus ID: 320191
• UniProt ID: Q8BUK6
• Cytogenetics: 8 A2
• Gene Summary: Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex (By similarity). Serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (PubMed:12950921). Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).[UniProtKB/Swiss-Prot Function]