Sdccag8 (NM_029756) Mouse Untagged Clone

CAT#: MC221021

Sdccag8 (untagged) - Mouse serologically defined colon cancer antigen 8 (Sdccag8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sdccag8
• Synonyms: 2700048G21Rik; 5730470G24Rik; Cccap
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221021 representing NM_029756
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGTCCCCAGGGAACTCTACCCTGGAGGACAGTCTGGGGCAATACCAACGGAGTCTCCGGGAAC
  GTGCCAATAGAAGCATTCACCAGTTGAAATGTGCCCTGAGAGAAGTGGATGTCACTGTAGAAGAAGATGC
  ACTGGATCCTTCCACCAGCATCAACGTAGAAAATGAGGACACAGGGGTGGCCTGGCATGAGCTGCAGCAC
  AGCCATGCTGTCAATCAACTGAAAGCTTTGTTGCGTCAACAAACAAATAAGGAAAATGAGACATCTCCAC
  CGCGAAGACGAAAGTTGTCCCCTTCGCGGCCGTCAGAATGTGACGATGGCAGCATGCCCACCATGCACAA
  CCTCGTTCCTATCATTAACGACCAGTCTCAATATATCCACCACTTAGAAGCAGAAGTCAAGTTCTGCAAG
  GATGAATTGTCTGGAATGAAAAATAGGGTGCAAGTAGTTGTACTTGAGAATGAAAGGCTCCAGCAAGAAC
  TGAAATCTCAAAGACCGGAGGAGACATTGAGGGAGCAAACATTCCTGGATGCATCTGGAAACATGCAGAA
  TTCTTGGATTATGACACGAGAAGATTCTAGGGTGGATGAAGCTGCAAAGAGACCATTTTCTCATGGTGAT
  GCTGAAACTGGCAAAACTGCATCCACTGGTGATGCCAATAAATGGAAGCTGGAGCTGGAGAGGCTGAAAC
  TCACATACGAGGCGAAGACTGACCTTCTGGAATCTCAGCTGATGCTTCTGAGGAAAGACTTAGCTGAATA
  CCAGAAAACCTGTGAGGATCTTAAGGAAAGATTGAAGCATAAAGAGTCTCTTCTGGCTGCGAGTGCATCC
  AGCCGTGTGGGTGGCCTCTGTTTGAAATGTGCTCAGCATGAAGCTGTTCTTTCCCAGACACACAGCAACG
  TGCACATCCAGACCATCGAGAGGCTGACTAAAGAGAGAGATGACTTAATGTCTGTGCTAGTCTCTGTGCG
  GAGCAGCTTGGCAGAAGCGCAGAAGAGAGAGACAAGTGCCTATGAGCAGGTGAAGCATGCTGTGCAGATG
  ACAGAGGAAGCCAACTTTGAGAAGACCAAGGCTTTGATCCAGTGTGAGCAGCTGAAGAGTGAGCTGGAGA
  GGCAGACAGAAAGACTGGAAAAGGAGCTTGCCTCTCAGCAAGAAAAGAGGGCTGTGGAGAAGGAGATGAT
  AAAGAAAGAAGTAGCCAGAGAGAGGGAAGATGCAGAGTCAAAGATGTTGATCCTGTCTCAGAATATTGCC
  AAGCTGGAGGCCCAGGTTGAAAAGGTCACCAGGGAGAAGACTGCAGCTGTCAGTCACTTAGAGGAAATTC
  AGAACCACGTTGCGTCTCAGGAAATGGACGTCACAAAGGTGTGTGGAGAAATGCGCTTTCAGTTGAATAA
  AACCAAAATGGAGAAGGATGAGGTGGAAAAGGAACACAGAGAATACAAAGCAAAGTCTCACAAGGATCTT
  GAAATGAAAGTCCAGGAAATAGAGAAGTTGAGACTAGAACTGAGCGAAAGCGAGCAGCATGTGGAACAGG
  AGCAGCAGAAGGCAGCTCGGGCCAGACAGGAGTGCCTGCGAGTCACAGAGCTGCTGGGCGAGGCCGAGCG
  CCAACTGCACCTCACCAGACTGGAAAAAGATAGCATTCAGCAAAGCTTTAGCAATGAAGCAAAGGCCCAA
  GCCCTTCAGGCCCAGCAGAGAGAACAGGAGCTGACACAGAAGATACAGCAAATGGAGACCCAGCACGACA
  AAACTGAAAGTGAACAGTATTTACTGCTGACATCCCAGAACACATTTCTGACAAAATTAAAAGAAGAATG
  CTGCTTGTTAGCCAAGAAACTGGAAAAGGTCTCACTAAAGAGCAGATCGCAAATAGTTCGACTTAGTCAA
  GAGAAGAGATACTTGTGTGACAAGCTAGAGAAGTTACAGAAGAGAAATGACGAACTAGAAGAGCAATGCA
  TCCAACATGGGAGGGTGCATGAGACCATGAAGGAAAGACTCAGGCAGCTGGATAAGCACGGCCAGGCTAC
  AGCGCAGCAGCTGGTGCAGTTGCTTAACAAGCAGAATCAGCTTCTCCTGGAGCGACAGAACCTGTCTGAG
  GAAGTGGCCCGGCTGCGAGCGCAGTTACCCAGCATGCCACAATCTGATTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029756
• Insert Size: 2154 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029756.3, NP_084032.1
• RefSeq Size: 2771 bp
• RefSeq ORF: 2154 bp
• Locus ID: 76816
• UniProt ID: Q80UF4
• Cytogenetics: 1 H4
• Gene Summary: Plays a role in the establishment of cell polarity and epithelial lumen formation (PubMed:20835237). Plays also an essential role in ciliogenesis and subsequent Hedgehog signaling pathway that requires the presence of intact primary cilia for pathway activation. Mechanistically, interacts with and mediates RABEP2 centrosomal localization which is critical for ciliogenesis (PubMed:27224062).[UniProtKB/Swiss-Prot Function]