Ccdc14 (BC031204) Mouse Untagged Clone

CAT#: MC220923

Ccdc14 (untagged) - Mouse coiled-coil domain containing 14 (cDNA clone MGC:37725 IMAGE:5066612), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc14
• Synonyms: G630039H03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGGTCCGGATCTCGACCGGGCCAGGTGATATCTTCAGGAAGGCAACCTGGAGCTAAGTTACCAA
  ATGGAAAGAAAGGGACCCACGTAAGAAAAATAGCACATTTTAATGTAGACTCCGCCTGTCCTGTTCAATC
  TGATTCAGAAAGTCAGACGGACATTCTCCAGGGACTTGACGGCTGTGCTTCTTTGCTGCGGGACATTTTG
  AAAAATGAAGATTCAGGCTCAGAAATGGTATATTCGGAAAACAGATGCAATTCTAAACCTTTAGAAGGCA
  GAAAGAACAGATCTAAAAAGAAAGGGCCTGAAAAATATGTTCCTTCAGTGGTCCGAAAGGAAATATTATC
  ATCAGAAAATAGGAAATGGATATCTAATGAAGTTTCTACTGGGAGTGACACAGCACAGCGTTGGTCACTA
  CAAGATCATTATAGAATGTACTCACCGATAATATACCAAGCCCTCTGTGAGCATGTGCAGACTCAAATGT
  CACTGATGAATAGCTTGGCTTTAAAAAATGGCCCCAATGGAATACATGCTGTGTGCCATACCATGTCTAA
  TTCAGGCTCTCAGGCAACACCACATTCTGGTTTTGGCTGTTCTTCCACTTCTGTCTGGCCACCTCAGCAG
  CCATCCTGCCCCCTAATGGTCCACTCTGAAGTTCAGACTGATGCTGATAACCAGTTTGAATCACAAAGTC
  AAGCGACCTCTGTGAAGTACGGTGATGTTCCCAGGGATCCGTTTAGTGTCCATCCTGGAGTTGCATGTGG
  CCTTCCCCACGCTGACAGTCCAGCTGTTGCAAAGAGTCGGCAGCTGGATCTTGCGAGTTGTATTCTGCCC
  CAACAGAGAGCTCCGAAGGAAGCAGAGTTACTAAAGTGCTTCCAAACACACACATCTCTGTTTCCAGCTC
  ACGGGAAGGACGCTCTCTCGGACAGTCAGGCATACCAAAGTCCCTCTCAGTTACAGCCAGCCTTCTTGGC
  CACTAATGAAGAAAAATGTGCCAAAGAACAATTTGAAGAGGCCACAAATGAAGGAAAGCATTTAAGCACA
  CATGTGCAAGATGCAAAAATAGCCAAGAATGTGCAGCAGGCAGAAAATGTGAGCCAGACTGCTGAAAAAA
  TCAGACTTGCCAAGTGCTTGTTGGGAGAGCTCAAGGCCCTGATGGAAGAGGACTCCGAAGTTCAGAGGTT
  GGTGACAGAAGTAGAGGCGTGCGTATCTGGACTTTCAGCAGTGAGTGGCCACCCAAATGTGGAAGTTGAA
  ATAGCCCTGGCCCTGCAGCCCCTGAGAAGTGAGAACGCTCAGCTGCGGAGGCAACTGAGAATTTTGAATC
  AACACCTCCGAGAACAAGAGAAAACCGAAAAGGGACCTGGTAACCTTGAATTGTTTTCCCTTCAGTCGCT
  GAATATGTCGCTGCAGAATCAGCTGCAAGAGTCGCTGAAGAGCCAGGAGTTACTACAGAGTAAAAACGAA
  GAGCTCTTAAAAGTGATAGAAAACCAGAAAGAGGAAAACAAAAAATTCACCACTTTATTTAAAGACAAAG
  AGCAGACTCTGCTTCATAATAAACAGCAGTTTGACATTGAGATGACCAGGGTGAAGATGGAGTTGGAGGA
  AGCCTTGGCCAATGAGAAGAACTTGCGGTTTAAGCTAGAGACTGCAGAAAAGGAAAGTCAGATACTGGGA
  ATAACACTGCGCCAGCGAGATGCTGAGGTTGCACGACTTAGAGAATTAACCAGAACTTTACAGAGCAGCA
  TGGCCAAGCTGCTCTCTGATCTCAGTATGGACACTGCACGCTGCAGGGTGGGGAATAGCCTCACCAAATC
  ACTTCTCAATGTCTATGATCAACAACTTCAACAGGACCCAGCCCCTGCTCAGACTTCCATAATGAGCTAC
  CTCAATAAATTAGAAACAAATCACAGCTTTATACATTCAAAGCTGCTTAACAATGAGGCAGCCATAGAGC
  CAGACAGACTCTGTGAAAGTGTGCTGCCCCCACAAAGGCCTCCCCATAGTGATGCGGGGACAGCAGATGG
  GGGCTCTGCATCTGGAACTGTCTCTGCTGCTTCAGTGGAGGATTCCGATATGGACTGTGAAACCCTCACT
  GTAATAGAAGATGGGTGTAACGTGGACAGTACAGTTTATATCCCCTTTGCAAGAAGCACTTCTAAAAAGC
  ACTCGCCACTTTGTAAGAGACTATCCCCTCAGCCAAAGCTGAGTGTTGCTGCAGCACAGCTAGTCAGCGG
  CAGTGCACAGACCTCTAAAAGAGAAAGTAAGCCACGTGCACCTGCAGTTTGTTCCTCACGAAATGAAGCA
  GAAGATGCACCCGGAGATCTTTCCAGCACGTATGACACAGAGGACGTGCAGCTCCTCAGGAAAATCAAAG
  AAGCTATTGGTAAGATCCCTGCTGCTGCTGAGCACGCAAAGGAACAGGCCACACATCATGGCCCATCAGC
  TCATCCCAGCCCCGGCGTTCCAGTGAAGGGCAGTATTGTTCCTGACAGCAGCTTTCTCAATTCTGACTTG
  ATGTCAGACTGGAGCATCTCCTCTTTCTCAACGTTCACGTCTCATGATGAACAGGACTTCAGAAACGGCC
  TGGCAGCACTGGATGCCAATATTGCTCGACTTCAGAAGTCCTTAAAGACCGGCCTTCTGGAGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC031204
• Insert Size: 2658 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031204, AAH31204
• RefSeq Size: 2884 bp
• RefSeq ORF: 2657 bp
• Locus ID: 239839
• Cytogenetics: 16 B3
• Gene Summary: Negatively regulates centriole duplication. Negatively regulates CEP63 and CDK2 centrosomal localization.[UniProtKB/Swiss-Prot Function]