Hps1 (NM_019424) Mouse Untagged Clone

CAT#: MC220890

Hps1 (untagged) - Mouse Hermansky-Pudlak syndrome 1 homolog (human) (Hps1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hps1
• Synonyms: 6030422N11Rik; BB405864; ep; Gm21361; Hps
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220890 representing NM_019424
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGCGTGTTGGTGGCCACCGAGGGCGCAGAGGTCCTCTTCTACTGGACAGATGAGGAGTTTGCTG
  AGAGCCTCCGGCTGAAGCTCCAGCAGTCAGAGGATGAGGAAGAGGAGCTCCCTGTGCTGGAGGACCAGCT
  CAGCACCCTCCTGGCCCCAGTCATCATCTCCTCCATGACGATGATGGAGAAGCTCTCGGACACATATACC
  TGCTTCTCAACAGAAAATGACAACCACCTGTATGTCCTGCATCTGTTTGGAGAGTACCTGTTCGTCGCCA
  TCAACGGAGACCACAGTGAGAGCGAGGGGGACCTGCGGAGGAAACTGTGTGTGCTCAAGTATCTATTCGA
  GGTGCACTTCGGGCTGGTGACTGTGGACGGCCAGCTCATCCGGAAGGAGCTTCGTCCACCCGACCTGGAA
  GAGCGTGCCCGGGTGTGGAAGCACTTTCAGAGACTGCTGGGGACCTACAGCTACCTGCGGGACCGGGAGC
  AGAGCTTTGCTGTGGAGGCAGTGGAGCGGCTCATTCACCCCCAGCTCTGCGAACAGAGCATCGAGACGCT
  AGAGCGGCACGTGGTTCAAGCCATCAACGCCAGCCCCGAGCGGGGCGGTGAAGAGGTCCTGCACGCCTTC
  CTGCTGGTGCACTGCAAGCTGTTGGCTTTCTACTCCGGCCATGGTGCGAGCACCCTGCGCCCCGCAGACC
  TCCTGGCGCTCATCCTTCTGGTGCAGGACCTCCAGCCCTCCCCAGGAACCACAGAGGAGGAGGAGGAGGA
  GGAGGATAGCGACAGCCCTCAGAGGAGACCCAAGAGCAGCCAAAACATCCCAGTGCAGCAGGCAAGGAGC
  CAGAGCACCTCAGTCCCCACCAGGAGTTCCAGGGAGACAGACACAGACAGCATCTCCCTCCCTGAAGAGT
  ACTTCACTCCTGCTCCTTCCCCAGGCGATCAGAGTTCAGGTAGCCTCGTCTGGCTGGATGGTGGCACCCC
  GCCCAGCGATGCTCTTCAGATGGCTGAGGACACCCCCGAGGGGCTGGCGTCTCACTCCCCAGAACTTCCC
  AGCCCCAGAAGGATCTTCCTGGATGCCAACATAAAAGAAAACTACTGTCCCTTAGTGCCCCACACCATGT
  ACTGCCTGCCCCTGTGGCCAGGCATCAACATGGTGCTGCTGACCAAGAGCCCCAGCACCCCACTGGCCCT
  GATCCTGTACCAACTGCTGGATGGCTTCTCCCTCCTGGAGAAAAAGCTGAAGGAAGGTCAGGAGGCCGGA
  AGTGCCCTGCGATCCCAGCCCTTTGTTGCAGACCTGCGCCAGAAGATGGACAAGTTTATCAAGAATCGAG
  TTGGGCAGGAGATTCAGAACACCTGGCTGGAGTTTAAGAGCAAAGCCTTCTCTAGAAGTGAGCCAGGATC
  GTCCTGGGAGCTGCTCCAGGTCTGTGGAAAGCTGAAGCGACAGCTGTGTGTCATCTACCGGCTTAGCTTC
  CTGGTCACTGCACCCAGCAGAGGAGGACCGCACCTGCCCCAGCACCTGCAGGACCGAGCCCAGAAACTCA
  TGAAGGAGAGGCTTCTGGACTGGAAGGACTTCCTGTTGGTGAAGAGCCGGAGAAATGTCACCATGGTGTC
  CTACCTGGAGGATTTCCCAGGCCTGGTCCACTTCATCTATGTGGACCGTACAACCGGGCAGATGGTGGCC
  CCCTCTCTCAGCCCCAACGAAAAGATGTCTTCCGAGTTGGGCAAGGGGCCCCTGGCTGCCTTTGTCAAAG
  CCAAGGTCTGGGCTCTGGTCCGACTGGCGCGCAGGTACCTGCAGAAGGGCTGCACCACACTGCTGTTCCA
  GGAAGGGGACTTCCGCTGCTCCTACTTCCTGTGGTTCGAGAATGACATGGGATACAAACTGCAGATGATT
  GAGGTGCCTGTCCTCTCGGATGACTCCGTCCCCATTGGCGTGCTGGGAGGTGACTACTACAGGAAACTTC
  TGCGCTACTACAGCAAGAGCCACCCCTCGGAGCCCGTCAGGTGCTATGAGCTGCTCACGCTGCATCTGTC
  CGTCATCCCCACGGACCTGCTGGTGCAGCAGGCCAGCCAGCTGGCCCGGCGCCTGGGGGAGGCCTCCCGG
  GTGACCCTGCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019424
• Insert Size: 2115 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019424.2, NP_062297.1
• RefSeq Size: 2800 bp
• RefSeq ORF: 2115 bp
• Locus ID: 192236
• UniProt ID: O08983
• Cytogenetics: 19 36.56 cM
• Gene Summary: Component of the BLOC-3 complex, a complex that acts as a guanine exchange factor (GEF) for RAB32 and RAB38, promotes the exchange of GDP to GTP, converting them from an inactive GDP-bound form into an active GTP-bound form. The BLOC-3 complex plays an important role in the control of melanin production and melanosome biogenesis and promotes the membrane localization of RAB32 and RAB38.[UniProtKB/Swiss-Prot Function]